Table 1. Open in new tab Primer...

Gene/Species

Forward/Reverse Primer Sequences

Reference Sequence

Angiotensinogen (mouse/rat)

F: 5′-CCACGCTCTCTGGATTTATC-3′

NM_007428.3

R: 5′-ACAGACACCGAGATGCTGTT-3′

HO-1 (mouse/rat)

F: 5′-CACCAAGTTCAAACAGCTCT-3′

NM_010442.2

R: 5′-CAGGAAACTGAGTGTGAGGA-3′

hnRNP F (mouse/rat)

F: 5′-AATTGTGCCAAACGGGATCA-3′

NM_133834.2

R: 5′-GCACCAGACCTCATCCTATCCA-3′

hnRNP K (mouse/rat)

F: 5′- CAGCTCCCGCTCGAATCTG-3′

NM_001301341.1

R: 5′- ACCCTATCAGGTTTTCCTCCAA-3′

KEAP1 (mouse/rat)

F: 5′-CATCCACCCTAAGGTCATGGA-3′

NM_016679.4

R: 5′-GACAGGTTGAAGAACTCCTCC-3′

Nrf2 (mouse/rat)

F: 5′-CGCCGCCTCACCTCTGCTGCCAGTAG-3′

NM_010902.3

R: 5′-AGCTCATAATCCTTCTGTCG-3′

Nox1 (mouse/rat)

F: 5′-GGTCACTCCCTTTGCTTCCA-3′

NM_172203.2

R: 5′- GGCAAAGGCACCTGTCTCTCT-3′

Nox2 (mouse/rat)

F: 5′-CCCTTTGGTACAGCCAGTGAAGAT-3′

NM_007807.5

R: 5′- CAATCCCGGCTCCCACTAACATCA-3′

Nox4 (mouse/rat)

F: 5′-TGGCCAACGAAGGGGTTAAA-3′

NM_015760.4

R: 5′-GATGAGGCTGCAGTTGAGGT-3′

β-Actin (mouse/rat)

F: 5′-ACGATTTCCCTCTCAGCTT-3′

NM_031144.3

R: 5′-TACAATGAGCTGCGTGTGGC-3′

hnRNP F gene promoter (rat)

F: 5′-AAAGGTACCTTTTTAAAGTCTTAAGCATTTG-3′

NC_005103.4

R: 5′-AAAAAGCTTCAGGGGAAACGCTTTTCG-3′

hnRNP K gene promoter (rat)

F: 5′-AAAGGTACCGGAGGCAACGGCGGACTCGC-3′

NC_005116.4

R: 5′-AAAAAGCTTACCAATTCACCATTGGTTTCGG-3′

Rat Nrf2 promoter

F: 5′-TAATTAGGTACCCTTGCCTCTTGCCCTAGCC-3′

−150

F: 5′-TAATTAGGTACCCCCGAACCACGAGAGGAGG-3′

−400

F: 5′-TAATTAGGTACCTTCGGCAAACAGCTGCTAATC-3′

−537

F: 5′-TAATTAGGTACCAGCGTGGACTCATCCATCTC-3′

−820

R: 5′-AAAAAACTCGAGTGCTGGGACTGTAGTCCTGGC-3′

+111

Rat Nrf2 promoter- hnRNP F/K-RE (N-607/-592)

F: 5′-CGATAGCAGCGCAGGTGTGTTTGCTC-3′

Site-directed mutagenesis primers

R: 5′-GAGCAAACACACCTGCGCTGCTATCG-3′

Rat Nrf2 promoter- hnRNP F/K-RE (N-463/-444)

F: 5′-CAAGGCCTCCTGCTACTTCAGCCCAC-3′

Site-directed mutagenesis primers

R: 5′-GTGGGCTGAAGTAGCAGGAGGCCTTG-3′

Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444)

F: 5′-CTCGCGCCCCGCCCCCGCGGGAC-3′

Biotinylated probe for EMSA

R: 5′-GTCCCGCGGGGGCGGGGCGCGAG-3′

Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444) WT

F: 5′-CTCGCGCCCCGCCCCCGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGGGGGCGGGGCGCGAG-3′

hnRNP F/K-RE (M1)

F: 5′-CTCGCGAAAAGCCCCCGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGGGGGCTTTTCGCGAG-3′

hnRNP F/K-RE (M2)

F: 5′-CTCGCGCCCAAACCCCGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGGGGTTTGGGCGCGAG-3′

hnRNP F/K-RE (M3)

F: 5′-CTCGCGCCCCGAAAACGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGTTTTCGGGGCGCGAG-3′

hnRNP F/K-RE (M4)

F: 5′-CTCGCGAAAAGAAAACGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGTTTTCTTTTCGCGAG-3′

Gene/Species

Forward/Reverse Primer Sequences

Reference Sequence

Angiotensinogen (mouse/rat)

F: 5′-CCACGCTCTCTGGATTTATC-3′

NM_007428.3

R: 5′-ACAGACACCGAGATGCTGTT-3′

HO-1 (mouse/rat)

F: 5′-CACCAAGTTCAAACAGCTCT-3′

NM_010442.2

R: 5′-CAGGAAACTGAGTGTGAGGA-3′

hnRNP F (mouse/rat)

F: 5′-AATTGTGCCAAACGGGATCA-3′

NM_133834.2

R: 5′-GCACCAGACCTCATCCTATCCA-3′

hnRNP K (mouse/rat)

F: 5′- CAGCTCCCGCTCGAATCTG-3′

NM_001301341.1

R: 5′- ACCCTATCAGGTTTTCCTCCAA-3′

KEAP1 (mouse/rat)

F: 5′-CATCCACCCTAAGGTCATGGA-3′

NM_016679.4

R: 5′-GACAGGTTGAAGAACTCCTCC-3′

Nrf2 (mouse/rat)

F: 5′-CGCCGCCTCACCTCTGCTGCCAGTAG-3′

NM_010902.3

R: 5′-AGCTCATAATCCTTCTGTCG-3′

Nox1 (mouse/rat)

F: 5′-GGTCACTCCCTTTGCTTCCA-3′

NM_172203.2

R: 5′- GGCAAAGGCACCTGTCTCTCT-3′

Nox2 (mouse/rat)

F: 5′-CCCTTTGGTACAGCCAGTGAAGAT-3′

NM_007807.5

R: 5′- CAATCCCGGCTCCCACTAACATCA-3′

Nox4 (mouse/rat)

F: 5′-TGGCCAACGAAGGGGTTAAA-3′

NM_015760.4

R: 5′-GATGAGGCTGCAGTTGAGGT-3′

β-Actin (mouse/rat)

F: 5′-ACGATTTCCCTCTCAGCTT-3′

NM_031144.3

R: 5′-TACAATGAGCTGCGTGTGGC-3′

hnRNP F gene promoter (rat)

F: 5′-AAAGGTACCTTTTTAAAGTCTTAAGCATTTG-3′

NC_005103.4

R: 5′-AAAAAGCTTCAGGGGAAACGCTTTTCG-3′

hnRNP K gene promoter (rat)

F: 5′-AAAGGTACCGGAGGCAACGGCGGACTCGC-3′

NC_005116.4

R: 5′-AAAAAGCTTACCAATTCACCATTGGTTTCGG-3′

Rat Nrf2 promoter

F: 5′-TAATTAGGTACCCTTGCCTCTTGCCCTAGCC-3′

−150

F: 5′-TAATTAGGTACCCCCGAACCACGAGAGGAGG-3′

−400

F: 5′-TAATTAGGTACCTTCGGCAAACAGCTGCTAATC-3′

−537

F: 5′-TAATTAGGTACCAGCGTGGACTCATCCATCTC-3′

−820

R: 5′-AAAAAACTCGAGTGCTGGGACTGTAGTCCTGGC-3′

+111

Rat Nrf2 promoter- hnRNP F/K-RE (N-607/-592)

F: 5′-CGATAGCAGCGCAGGTGTGTTTGCTC-3′

Site-directed mutagenesis primers

R: 5′-GAGCAAACACACCTGCGCTGCTATCG-3′

Rat Nrf2 promoter- hnRNP F/K-RE (N-463/-444)

F: 5′-CAAGGCCTCCTGCTACTTCAGCCCAC-3′

Site-directed mutagenesis primers

R: 5′-GTGGGCTGAAGTAGCAGGAGGCCTTG-3′

Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444)

F: 5′-CTCGCGCCCCGCCCCCGCGGGAC-3′

Biotinylated probe for EMSA

R: 5′-GTCCCGCGGGGGCGGGGCGCGAG-3′

Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444) WT

F: 5′-CTCGCGCCCCGCCCCCGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGGGGGCGGGGCGCGAG-3′

hnRNP F/K-RE (M1)

F: 5′-CTCGCGAAAAGCCCCCGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGGGGGCTTTTCGCGAG-3′

hnRNP F/K-RE (M2)

F: 5′-CTCGCGCCCAAACCCCGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGGGGTTTGGGCGCGAG-3′

hnRNP F/K-RE (M3)

F: 5′-CTCGCGCCCCGAAAACGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGTTTTCGGGGCGCGAG-3′

hnRNP F/K-RE (M4)

F: 5′-CTCGCGAAAAGAAAACGCGGGAC-3′

Competitor

R: 5′-GTCCCGCGTTTTCTTTTCGCGAG-3′

Table 1.

Open in new tab

Primer Sequences for RT-qPCR, Subcloning, and EMSA

Gene/Species	Forward/Reverse Primer Sequences	Reference Sequence
Angiotensinogen (mouse/rat)	F: 5′-CCACGCTCTCTGGATTTATC-3′	NM_007428.3
Angiotensinogen (mouse/rat)	R: 5′-ACAGACACCGAGATGCTGTT-3′	NM_007428.3
HO-1 (mouse/rat)	F: 5′-CACCAAGTTCAAACAGCTCT-3′	NM_010442.2
HO-1 (mouse/rat)	R: 5′-CAGGAAACTGAGTGTGAGGA-3′	NM_010442.2
hnRNP F (mouse/rat)	F: 5′-AATTGTGCCAAACGGGATCA-3′	NM_133834.2
hnRNP F (mouse/rat)	R: 5′-GCACCAGACCTCATCCTATCCA-3′	NM_133834.2
hnRNP K (mouse/rat)	F: 5′- CAGCTCCCGCTCGAATCTG-3′	NM_001301341.1
hnRNP K (mouse/rat)	R: 5′- ACCCTATCAGGTTTTCCTCCAA-3′	NM_001301341.1
KEAP1 (mouse/rat)	F: 5′-CATCCACCCTAAGGTCATGGA-3′	NM_016679.4
KEAP1 (mouse/rat)	R: 5′-GACAGGTTGAAGAACTCCTCC-3′	NM_016679.4
Nrf2 (mouse/rat)	F: 5′-CGCCGCCTCACCTCTGCTGCCAGTAG-3′	NM_010902.3
Nrf2 (mouse/rat)	R: 5′-AGCTCATAATCCTTCTGTCG-3′	NM_010902.3
Nox1 (mouse/rat)	F: 5′-GGTCACTCCCTTTGCTTCCA-3′	NM_172203.2
Nox1 (mouse/rat)	R: 5′- GGCAAAGGCACCTGTCTCTCT-3′	NM_172203.2
Nox2 (mouse/rat)	F: 5′-CCCTTTGGTACAGCCAGTGAAGAT-3′	NM_007807.5
Nox2 (mouse/rat)	R: 5′- CAATCCCGGCTCCCACTAACATCA-3′	NM_007807.5
Nox4 (mouse/rat)	F: 5′-TGGCCAACGAAGGGGTTAAA-3′	NM_015760.4
Nox4 (mouse/rat)	R: 5′-GATGAGGCTGCAGTTGAGGT-3′	NM_015760.4
β-Actin (mouse/rat)	F: 5′-ACGATTTCCCTCTCAGCTT-3′	NM_031144.3
β-Actin (mouse/rat)	R: 5′-TACAATGAGCTGCGTGTGGC-3′	NM_031144.3
hnRNP F gene promoter (rat)	F: 5′-AAAGGTACCTTTTTAAAGTCTTAAGCATTTG-3′	NC_005103.4
hnRNP F gene promoter (rat)	R: 5′-AAAAAGCTTCAGGGGAAACGCTTTTCG-3′	NC_005103.4
hnRNP K gene promoter (rat)	F: 5′-AAAGGTACCGGAGGCAACGGCGGACTCGC-3′	NC_005116.4
hnRNP K gene promoter (rat)	R: 5′-AAAAAGCTTACCAATTCACCATTGGTTTCGG-3′	NC_005116.4
Rat Nrf2 promoter	F: 5′-TAATTAGGTACCCTTGCCTCTTGCCCTAGCC-3′	−150
	F: 5′-TAATTAGGTACCCCCGAACCACGAGAGGAGG-3′	−400
	F: 5′-TAATTAGGTACCTTCGGCAAACAGCTGCTAATC-3′	−537
	F: 5′-TAATTAGGTACCAGCGTGGACTCATCCATCTC-3′	−820
	R: 5′-AAAAAACTCGAGTGCTGGGACTGTAGTCCTGGC-3′	+111
Rat Nrf2 promoter- hnRNP F/K-RE (N-607/-592)	F: 5′-CGATAGCAGCGCAGGTGTGTTTGCTC-3′	Site-directed mutagenesis primers
Rat Nrf2 promoter- hnRNP F/K-RE (N-607/-592)	R: 5′-GAGCAAACACACCTGCGCTGCTATCG-3′	Site-directed mutagenesis primers
Rat Nrf2 promoter- hnRNP F/K-RE (N-463/-444)	F: 5′-CAAGGCCTCCTGCTACTTCAGCCCAC-3′	Site-directed mutagenesis primers
Rat Nrf2 promoter- hnRNP F/K-RE (N-463/-444)	R: 5′-GTGGGCTGAAGTAGCAGGAGGCCTTG-3′	Site-directed mutagenesis primers
Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444)	F: 5′-CTCGCGCCCCGCCCCCGCGGGAC-3′	Biotinylated probe for EMSA
Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444)	R: 5′-GTCCCGCGGGGGCGGGGCGCGAG-3′	Biotinylated probe for EMSA
Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444) WT	F: 5′-CTCGCGCCCCGCCCCCGCGGGAC-3′	Competitor
Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444) WT	R: 5′-GTCCCGCGGGGGCGGGGCGCGAG-3′	Competitor
hnRNP F/K-RE (M1)	F: 5′-CTCGCGAAAAGCCCCCGCGGGAC-3′	Competitor
hnRNP F/K-RE (M1)	R: 5′-GTCCCGCGGGGGCTTTTCGCGAG-3′	Competitor
hnRNP F/K-RE (M2)	F: 5′-CTCGCGCCCAAACCCCGCGGGAC-3′	Competitor
hnRNP F/K-RE (M2)	R: 5′-GTCCCGCGGGGTTTGGGCGCGAG-3′	Competitor
hnRNP F/K-RE (M3)	F: 5′-CTCGCGCCCCGAAAACGCGGGAC-3′	Competitor
hnRNP F/K-RE (M3)	R: 5′-GTCCCGCGTTTTCGGGGCGCGAG-3′	Competitor
hnRNP F/K-RE (M4)	F: 5′-CTCGCGAAAAGAAAACGCGGGAC-3′	Competitor
hnRNP F/K-RE (M4)	R: 5′-GTCCCGCGTTTTCTTTTCGCGAG-3′	Competitor

Gene/Species	Forward/Reverse Primer Sequences	Reference Sequence
Angiotensinogen (mouse/rat)	F: 5′-CCACGCTCTCTGGATTTATC-3′	NM_007428.3
Angiotensinogen (mouse/rat)	R: 5′-ACAGACACCGAGATGCTGTT-3′	NM_007428.3
HO-1 (mouse/rat)	F: 5′-CACCAAGTTCAAACAGCTCT-3′	NM_010442.2
HO-1 (mouse/rat)	R: 5′-CAGGAAACTGAGTGTGAGGA-3′	NM_010442.2
hnRNP F (mouse/rat)	F: 5′-AATTGTGCCAAACGGGATCA-3′	NM_133834.2
hnRNP F (mouse/rat)	R: 5′-GCACCAGACCTCATCCTATCCA-3′	NM_133834.2
hnRNP K (mouse/rat)	F: 5′- CAGCTCCCGCTCGAATCTG-3′	NM_001301341.1
hnRNP K (mouse/rat)	R: 5′- ACCCTATCAGGTTTTCCTCCAA-3′	NM_001301341.1
KEAP1 (mouse/rat)	F: 5′-CATCCACCCTAAGGTCATGGA-3′	NM_016679.4
KEAP1 (mouse/rat)	R: 5′-GACAGGTTGAAGAACTCCTCC-3′	NM_016679.4
Nrf2 (mouse/rat)	F: 5′-CGCCGCCTCACCTCTGCTGCCAGTAG-3′	NM_010902.3
Nrf2 (mouse/rat)	R: 5′-AGCTCATAATCCTTCTGTCG-3′	NM_010902.3
Nox1 (mouse/rat)	F: 5′-GGTCACTCCCTTTGCTTCCA-3′	NM_172203.2
Nox1 (mouse/rat)	R: 5′- GGCAAAGGCACCTGTCTCTCT-3′	NM_172203.2
Nox2 (mouse/rat)	F: 5′-CCCTTTGGTACAGCCAGTGAAGAT-3′	NM_007807.5
Nox2 (mouse/rat)	R: 5′- CAATCCCGGCTCCCACTAACATCA-3′	NM_007807.5
Nox4 (mouse/rat)	F: 5′-TGGCCAACGAAGGGGTTAAA-3′	NM_015760.4
Nox4 (mouse/rat)	R: 5′-GATGAGGCTGCAGTTGAGGT-3′	NM_015760.4
β-Actin (mouse/rat)	F: 5′-ACGATTTCCCTCTCAGCTT-3′	NM_031144.3
β-Actin (mouse/rat)	R: 5′-TACAATGAGCTGCGTGTGGC-3′	NM_031144.3
hnRNP F gene promoter (rat)	F: 5′-AAAGGTACCTTTTTAAAGTCTTAAGCATTTG-3′	NC_005103.4
hnRNP F gene promoter (rat)	R: 5′-AAAAAGCTTCAGGGGAAACGCTTTTCG-3′	NC_005103.4
hnRNP K gene promoter (rat)	F: 5′-AAAGGTACCGGAGGCAACGGCGGACTCGC-3′	NC_005116.4
hnRNP K gene promoter (rat)	R: 5′-AAAAAGCTTACCAATTCACCATTGGTTTCGG-3′	NC_005116.4
Rat Nrf2 promoter	F: 5′-TAATTAGGTACCCTTGCCTCTTGCCCTAGCC-3′	−150
	F: 5′-TAATTAGGTACCCCCGAACCACGAGAGGAGG-3′	−400
	F: 5′-TAATTAGGTACCTTCGGCAAACAGCTGCTAATC-3′	−537
	F: 5′-TAATTAGGTACCAGCGTGGACTCATCCATCTC-3′	−820
	R: 5′-AAAAAACTCGAGTGCTGGGACTGTAGTCCTGGC-3′	+111
Rat Nrf2 promoter- hnRNP F/K-RE (N-607/-592)	F: 5′-CGATAGCAGCGCAGGTGTGTTTGCTC-3′	Site-directed mutagenesis primers
Rat Nrf2 promoter- hnRNP F/K-RE (N-607/-592)	R: 5′-GAGCAAACACACCTGCGCTGCTATCG-3′	Site-directed mutagenesis primers
Rat Nrf2 promoter- hnRNP F/K-RE (N-463/-444)	F: 5′-CAAGGCCTCCTGCTACTTCAGCCCAC-3′	Site-directed mutagenesis primers
Rat Nrf2 promoter- hnRNP F/K-RE (N-463/-444)	R: 5′-GTGGGCTGAAGTAGCAGGAGGCCTTG-3′	Site-directed mutagenesis primers
Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444)	F: 5′-CTCGCGCCCCGCCCCCGCGGGAC-3′	Biotinylated probe for EMSA
Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444)	R: 5′-GTCCCGCGGGGGCGGGGCGCGAG-3′	Biotinylated probe for EMSA
Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444) WT	F: 5′-CTCGCGCCCCGCCCCCGCGGGAC-3′	Competitor
Rat Nrf2 promoter hnRNP F/K-RE (N-463/-444) WT	R: 5′-GTCCCGCGGGGGCGGGGCGCGAG-3′	Competitor
hnRNP F/K-RE (M1)	F: 5′-CTCGCGAAAAGCCCCCGCGGGAC-3′	Competitor
hnRNP F/K-RE (M1)	R: 5′-GTCCCGCGGGGGCTTTTCGCGAG-3′	Competitor
hnRNP F/K-RE (M2)	F: 5′-CTCGCGCCCAAACCCCGCGGGAC-3′	Competitor
hnRNP F/K-RE (M2)	R: 5′-GTCCCGCGGGGTTTGGGCGCGAG-3′	Competitor
hnRNP F/K-RE (M3)	F: 5′-CTCGCGCCCCGAAAACGCGGGAC-3′	Competitor
hnRNP F/K-RE (M3)	R: 5′-GTCCCGCGTTTTCGGGGCGCGAG-3′	Competitor
hnRNP F/K-RE (M4)	F: 5′-CTCGCGAAAAGAAAACGCGGGAC-3′	Competitor
hnRNP F/K-RE (M4)	R: 5′-GTCCCGCGTTTTCTTTTCGCGAG-3′	Competitor

Boldface letters indicate the nucleotides replacing the nucleotides in WT hnRNP F/K-RE.

Abbreviations: HO-1, heme oxygenase-1; Keap1, Kelch-like ECH-associated protein 1; RE, responsive element; RT-qPCR, real-time quantitative polymerase chain reaction.

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