Table 1
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Antibody Table

Peptide/Protein TargetAntigen Sequence (if Known)Name of AntibodyManufacturer, Catalog Number, and/or Name of Individual Providing the AntibodySpecies Raised in; Monoclonal or PolyclonalDilution Used
Amino acids 781–1080 of GLI-1 of human originGLI1sc-20687, Santa Cruz Biotechnology, IncRabbit; polyclonal1:200
Peptide corresponding to human Gli2RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTEGLI2ab26056, Abcam LtdRabbit; polyclonal1:2000
Synthetic peptide conjugated to KLH derived from within residues 1–100 of human Gli3GLI3ab69838, Abcam LtdRabbit; polyclonal1:2000
DYKDDDDK tag (D6W5B)FLAG14793, Cell Signaling TechnologyRabbit; monoclonal1:2000
His-probe antibody (H-15)HISsc-803, Biotechnology, IncRabbit; polyclonal1:1000
Amino acids 261–405 mapping at the C terminus of 11β-HSD2 of human origin11β-HSD2sc-20176, Biotechnology, IncRabbit; polyclonal1:1000
Epitope mapping within the tandem repeat domain of the large subunit of RNA polymerase II (RPB1) of mouse originPOL-IIsc-900, Biotechnology, IncRabbit; polyclonal1:1000
A slightly modified synthetic peptide corresponding to β-cytoplasmic actinβ-Actinsc-69879, Biotechnology, IncMouse; monoclonal1:1000
Peptide/Protein TargetAntigen Sequence (if Known)Name of AntibodyManufacturer, Catalog Number, and/or Name of Individual Providing the AntibodySpecies Raised in; Monoclonal or PolyclonalDilution Used
Amino acids 781–1080 of GLI-1 of human originGLI1sc-20687, Santa Cruz Biotechnology, IncRabbit; polyclonal1:200
Peptide corresponding to human Gli2RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTEGLI2ab26056, Abcam LtdRabbit; polyclonal1:2000
Synthetic peptide conjugated to KLH derived from within residues 1–100 of human Gli3GLI3ab69838, Abcam LtdRabbit; polyclonal1:2000
DYKDDDDK tag (D6W5B)FLAG14793, Cell Signaling TechnologyRabbit; monoclonal1:2000
His-probe antibody (H-15)HISsc-803, Biotechnology, IncRabbit; polyclonal1:1000
Amino acids 261–405 mapping at the C terminus of 11β-HSD2 of human origin11β-HSD2sc-20176, Biotechnology, IncRabbit; polyclonal1:1000
Epitope mapping within the tandem repeat domain of the large subunit of RNA polymerase II (RPB1) of mouse originPOL-IIsc-900, Biotechnology, IncRabbit; polyclonal1:1000
A slightly modified synthetic peptide corresponding to β-cytoplasmic actinβ-Actinsc-69879, Biotechnology, IncMouse; monoclonal1:1000
Table 1
Open in new tab

Antibody Table

Peptide/Protein TargetAntigen Sequence (if Known)Name of AntibodyManufacturer, Catalog Number, and/or Name of Individual Providing the AntibodySpecies Raised in; Monoclonal or PolyclonalDilution Used
Amino acids 781–1080 of GLI-1 of human originGLI1sc-20687, Santa Cruz Biotechnology, IncRabbit; polyclonal1:200
Peptide corresponding to human Gli2RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTEGLI2ab26056, Abcam LtdRabbit; polyclonal1:2000
Synthetic peptide conjugated to KLH derived from within residues 1–100 of human Gli3GLI3ab69838, Abcam LtdRabbit; polyclonal1:2000
DYKDDDDK tag (D6W5B)FLAG14793, Cell Signaling TechnologyRabbit; monoclonal1:2000
His-probe antibody (H-15)HISsc-803, Biotechnology, IncRabbit; polyclonal1:1000
Amino acids 261–405 mapping at the C terminus of 11β-HSD2 of human origin11β-HSD2sc-20176, Biotechnology, IncRabbit; polyclonal1:1000
Epitope mapping within the tandem repeat domain of the large subunit of RNA polymerase II (RPB1) of mouse originPOL-IIsc-900, Biotechnology, IncRabbit; polyclonal1:1000
A slightly modified synthetic peptide corresponding to β-cytoplasmic actinβ-Actinsc-69879, Biotechnology, IncMouse; monoclonal1:1000
Peptide/Protein TargetAntigen Sequence (if Known)Name of AntibodyManufacturer, Catalog Number, and/or Name of Individual Providing the AntibodySpecies Raised in; Monoclonal or PolyclonalDilution Used
Amino acids 781–1080 of GLI-1 of human originGLI1sc-20687, Santa Cruz Biotechnology, IncRabbit; polyclonal1:200
Peptide corresponding to human Gli2RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTEGLI2ab26056, Abcam LtdRabbit; polyclonal1:2000
Synthetic peptide conjugated to KLH derived from within residues 1–100 of human Gli3GLI3ab69838, Abcam LtdRabbit; polyclonal1:2000
DYKDDDDK tag (D6W5B)FLAG14793, Cell Signaling TechnologyRabbit; monoclonal1:2000
His-probe antibody (H-15)HISsc-803, Biotechnology, IncRabbit; polyclonal1:1000
Amino acids 261–405 mapping at the C terminus of 11β-HSD2 of human origin11β-HSD2sc-20176, Biotechnology, IncRabbit; polyclonal1:1000
Epitope mapping within the tandem repeat domain of the large subunit of RNA polymerase II (RPB1) of mouse originPOL-IIsc-900, Biotechnology, IncRabbit; polyclonal1:1000
A slightly modified synthetic peptide corresponding to β-cytoplasmic actinβ-Actinsc-69879, Biotechnology, IncMouse; monoclonal1:1000
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