Table 3 Open in new tab Two-Way ANOVA...

Gene

P Value Treatment

P Value Treatment* Stage

H Transcript Level (SD)

Fold Change

FF Transcript Level (SD)

Fold Change

3wFF Transcript Level (SD)

Fold Change

IGF-II

<.0001

1.56 (0.59)

0.41

1.35 (0.45)

0.26

1.46 (0.52)

0.23

IGFBP-1A2

<.0001

0.13 (0.03)

0.75

0.21 (0.05)

0.66

0.15 (0.06)

0.23

IGFBP-1B2^a

<.0001

0.04 (0.02)

0.18

0.05 (0.03)

0.04

0.09 (0.11)

0.03

IGFBP-2B2^b

<.0001

0.04 (0.02)

0.26

1.63E-03 (0.00)

0.02

0.04 (0.02)

0.82

IGFBP-3B1

<.0001

0.92 (0.29)

0.98

0.91 (0.33)

0.59

0.58 (0.16)

0.30

IGFBP-1B1^a

<.0001

0.06 (0.05)

0.14

0.05 (0.05)

0.02

0.09 (0.08)

0.02

IGFBP-6B2

<.0001

0.48 (0.09)

0.48

0.98 (0.13)

0.39

1.91 (0.61)

0.36

IGFBP-3B2^b

<.0001

.002

0.11 (0.03)

0.68

0.14 (0.02)

0.61

0.06 (0.02)

0.33

IGFBP-6A2^c

<.0001

.002

0.85 (0.70)

13.76

5.86 (2.81)

64.36

0.74 (0.83)

8.25

MyoD1a

<.0001

.03^d

1.63 (0.91)

0.46

0.79 (0.30)

0.24

3.01 (1.02)

0.90

IGFBP-2B1

<.0001

.04^d

0.46 (0.06)

0.72

0.36 (0.15)

0.35

0.64 (0.30)

0.55

IGF-I

<.0001

.04^d

0.37 (0.11)

0.74

0.65 (0.11)

0.59

0.69 (0.16)

0.69

IGFBP-4

<.0001

.10

2.17 (0.51)

0.70

3.38 (0.82)

0.63

2.79 (0.60)

0.56

IGFBP-3A1

<.0001

.13

0.48 (0.14)

0.70

0.70 (0.11)

0.74

0.54 (0.05)

0.53

IGFBP-5A^b

<.0001

.23

2.01 (0.32)

0.73

2.81 (0.37)

0.58

3.77 (0.71)

0.59

MYOG

<.0001

.58

3.01 (1.29)

0.72

1.08 (0.31)

0.42

2.40 (0.88)

0.55

IGFBP-2A

<.0001

.75

6.36 (1.02)

0.77

8.21 (1.48)

0.84

6.27 (1.23)

0.73

IGFBP-3A2^b

<.0001

.75

0.09 (0.04)

0.38

0.25 (0.02)

0.49

0.34 (0.09)

0.47

FBXO32^b

.001

.10

0.17 (0.04)

1.16

1.08 (0.30)

2.48

25.39 (13.35)

2.06

IGFBP-5B1^b

.004^d

.10

2.09 (0.45)

4.11 (0.73)

5.47 (0.51)

IGFBP-5B2

.01^d

.20

2.38 (0.74)

4.06 (0.60)

4.66 (0.99)

IGFBP-1A1^b

.04^d

.54

0.66 (0.55)

3.11 (0.72)

3.22 (2.51)

MYL1

.17

.01^d

241.13 (37.48)

474.44 (15.23)

348.03 (59.98)

TNNI2^b

.25

.72

139.76 (26.72)

507.56 (38.03)

498.94 (283.79)

IGFBP-6B1^a

.65

.57

0.02 (0.02)

0.04 (0.02)

0.10 (0.04)

IGFBP-6A1

Table 3

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Two-Way ANOVA Testing the Effect of VHSv Challenge on Expression of the IGF-IGFBP System and Muscle Growth Genes in Rainbow Trout at Different Developmental Stages

Gene	P Value Treatment	P Value Treatment* Stage	H Transcript Level (SD)	Fold Change	FF Transcript Level (SD)	Fold Change	3wFF Transcript Level (SD)	Fold Change
IGF-II	<.0001	<.0001	1.56 (0.59)	0.41	1.35 (0.45)	0.26	1.46 (0.52)	0.23
IGFBP-1A2	<.0001	<.0001	0.13 (0.03)	0.75	0.21 (0.05)	0.66	0.15 (0.06)	0.23
IGFBP-1B2^a	<.0001	<.0001	0.04 (0.02)	0.18	0.05 (0.03)	0.04	0.09 (0.11)	0.03
IGFBP-2B2^b	<.0001	<.0001	0.04 (0.02)	0.26	1.63E-03 (0.00)	0.02	0.04 (0.02)	0.82
IGFBP-3B1	<.0001	<.0001	0.92 (0.29)	0.98	0.91 (0.33)	0.59	0.58 (0.16)	0.30
IGFBP-1B1^a	<.0001	<.0001	0.06 (0.05)	0.14	0.05 (0.05)	0.02	0.09 (0.08)	0.02
IGFBP-6B2	<.0001	<.0001	0.48 (0.09)	0.48	0.98 (0.13)	0.39	1.91 (0.61)	0.36
IGFBP-3B2^b	<.0001	.002	0.11 (0.03)	0.68	0.14 (0.02)	0.61	0.06 (0.02)	0.33
IGFBP-6A2^c	<.0001	.002	0.85 (0.70)	13.76	5.86 (2.81)	64.36	0.74 (0.83)	8.25
MyoD1a	<.0001	.03^d	1.63 (0.91)	0.46	0.79 (0.30)	0.24	3.01 (1.02)	0.90
IGFBP-2B1	<.0001	.04^d	0.46 (0.06)	0.72	0.36 (0.15)	0.35	0.64 (0.30)	0.55
IGF-I	<.0001	.04^d	0.37 (0.11)	0.74	0.65 (0.11)	0.59	0.69 (0.16)	0.69
IGFBP-4	<.0001	.10	2.17 (0.51)	0.70	3.38 (0.82)	0.63	2.79 (0.60)	0.56
IGFBP-3A1	<.0001	.13	0.48 (0.14)	0.70	0.70 (0.11)	0.74	0.54 (0.05)	0.53
IGFBP-5A^b	<.0001	.23	2.01 (0.32)	0.73	2.81 (0.37)	0.58	3.77 (0.71)	0.59
MYOG	<.0001	.58	3.01 (1.29)	0.72	1.08 (0.31)	0.42	2.40 (0.88)	0.55
IGFBP-2A	<.0001	.75	6.36 (1.02)	0.77	8.21 (1.48)	0.84	6.27 (1.23)	0.73
IGFBP-3A2^b	<.0001	.75	0.09 (0.04)	0.38	0.25 (0.02)	0.49	0.34 (0.09)	0.47
FBXO32^b	.001	.10	0.17 (0.04)	1.16	1.08 (0.30)	2.48	25.39 (13.35)	2.06
IGFBP-5B1^b	.004^d	.10	2.09 (0.45)		4.11 (0.73)		5.47 (0.51)
IGFBP-5B2	.01^d	.20	2.38 (0.74)		4.06 (0.60)		4.66 (0.99)
IGFBP-1A1^b	.04^d	.54	0.66 (0.55)		3.11 (0.72)		3.22 (2.51)
MYL1	.17	.01^d	241.13 (37.48)		474.44 (15.23)		348.03 (59.98)
TNNI2^b	.25	.72	139.76 (26.72)		507.56 (38.03)		498.94 (283.79)
IGFBP-6B1^a	.65	.57	0.02 (0.02)		0.04 (0.02)		0.10 (0.04)
IGFBP-6A1	ND	ND	ND		ND		ND

Gene	P Value Treatment	P Value Treatment* Stage	H Transcript Level (SD)	Fold Change	FF Transcript Level (SD)	Fold Change	3wFF Transcript Level (SD)	Fold Change
IGF-II	<.0001	<.0001	1.56 (0.59)	0.41	1.35 (0.45)	0.26	1.46 (0.52)	0.23
IGFBP-1A2	<.0001	<.0001	0.13 (0.03)	0.75	0.21 (0.05)	0.66	0.15 (0.06)	0.23
IGFBP-1B2^a	<.0001	<.0001	0.04 (0.02)	0.18	0.05 (0.03)	0.04	0.09 (0.11)	0.03
IGFBP-2B2^b	<.0001	<.0001	0.04 (0.02)	0.26	1.63E-03 (0.00)	0.02	0.04 (0.02)	0.82
IGFBP-3B1	<.0001	<.0001	0.92 (0.29)	0.98	0.91 (0.33)	0.59	0.58 (0.16)	0.30
IGFBP-1B1^a	<.0001	<.0001	0.06 (0.05)	0.14	0.05 (0.05)	0.02	0.09 (0.08)	0.02
IGFBP-6B2	<.0001	<.0001	0.48 (0.09)	0.48	0.98 (0.13)	0.39	1.91 (0.61)	0.36
IGFBP-3B2^b	<.0001	.002	0.11 (0.03)	0.68	0.14 (0.02)	0.61	0.06 (0.02)	0.33
IGFBP-6A2^c	<.0001	.002	0.85 (0.70)	13.76	5.86 (2.81)	64.36	0.74 (0.83)	8.25
MyoD1a	<.0001	.03^d	1.63 (0.91)	0.46	0.79 (0.30)	0.24	3.01 (1.02)	0.90
IGFBP-2B1	<.0001	.04^d	0.46 (0.06)	0.72	0.36 (0.15)	0.35	0.64 (0.30)	0.55
IGF-I	<.0001	.04^d	0.37 (0.11)	0.74	0.65 (0.11)	0.59	0.69 (0.16)	0.69
IGFBP-4	<.0001	.10	2.17 (0.51)	0.70	3.38 (0.82)	0.63	2.79 (0.60)	0.56
IGFBP-3A1	<.0001	.13	0.48 (0.14)	0.70	0.70 (0.11)	0.74	0.54 (0.05)	0.53
IGFBP-5A^b	<.0001	.23	2.01 (0.32)	0.73	2.81 (0.37)	0.58	3.77 (0.71)	0.59
MYOG	<.0001	.58	3.01 (1.29)	0.72	1.08 (0.31)	0.42	2.40 (0.88)	0.55
IGFBP-2A	<.0001	.75	6.36 (1.02)	0.77	8.21 (1.48)	0.84	6.27 (1.23)	0.73
IGFBP-3A2^b	<.0001	.75	0.09 (0.04)	0.38	0.25 (0.02)	0.49	0.34 (0.09)	0.47
FBXO32^b	.001	.10	0.17 (0.04)	1.16	1.08 (0.30)	2.48	25.39 (13.35)	2.06
IGFBP-5B1^b	.004^d	.10	2.09 (0.45)		4.11 (0.73)		5.47 (0.51)
IGFBP-5B2	.01^d	.20	2.38 (0.74)		4.06 (0.60)		4.66 (0.99)
IGFBP-1A1^b	.04^d	.54	0.66 (0.55)		3.11 (0.72)		3.22 (2.51)
MYL1	.17	.01^d	241.13 (37.48)		474.44 (15.23)		348.03 (59.98)
TNNI2^b	.25	.72	139.76 (26.72)		507.56 (38.03)		498.94 (283.79)
IGFBP-6B1^a	.65	.57	0.02 (0.02)		0.04 (0.02)		0.10 (0.04)
IGFBP-6A1	ND	ND	ND		ND		ND

Transcript levels shown are for infected animals. ND, no expression detected; fold change, mean transcript level at VHSv challenge/control.

Data transformed by square root.

Data transformed by natural logarithm.

Data transformed by double square root.

Nonsignificant differences after Bonferroni correction.

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