Expression of Genes Involved in Inflammation and Tissue Remodeling in Fetal Liver of the Groups LIG, SOP, and C on Gestational Days E19, E20, and E22
| Gene . | Day . | LIG . | SOP . | C . | ANOVA . | LIG-C . | LIG-SOP . | SOP-C . |
|---|---|---|---|---|---|---|---|---|
| Tgfb1 | E19 | 1.85 ± 0.10 | 2.00 ± 0.12 | 1.68 ± 0.16 | 0.226 | — | — | — |
| E20 | 1.50 ± 0.11 | 1.34 ± 0.13 | 1.53 ± 0.10 | 0.470 | — | — | — | |
| E22 | 1.22 ± 0.08 | 1.10 ± 0.08 | 1.00 ± 0.09 | 0.142 | — | — | — | |
| Pai1 | E19 | 0.86 ± 0.09 | 1.10 ± 0.13 | 0.24 ± 0.03 | <0.001 | a | — | a |
| E20 | 1.31 ± 0.23 | 0.45 ± 0.08 | 0.26 ± 0.04 | <0.001 | a | b | ns | |
| E22 | 1.29 ± 0.25 | 0.85 ± 0.11 | 1.00 ± 0.17 | 0.599 | — | — | — | |
| IL10 | E19 | 1.23 ± 0.18 | 0.81 ± 0.04 | 0.69 ± 0.08 | 0.009 | c | c | — |
| E20 | 0.84 ± 0.04 | 0.79 ± 0.04 | 0.74 ± 0.04 | 0.236 | — | — | — | |
| E22 | 1.16 ± 0.07 | 1.15 ± 0.07 | 1.00 ± 0.07 | 0.274 | — | — | — | |
| Mmp2 | E19 | 1.08 ± 0.08 | 1.14 ± 0.10 | 0.99 ± 0.09 | 0.558 | — | — | — |
| E20 | 0.46 ± 0.05 | 0.51 ± 0.07 | 0.64 ± 0.09 | 0.167 | — | — | — | |
| E22 | 1.23 ± 0.08 | 1.26 ± 0.07 | 1.00 ± 0.07 | 0.016 | — | — | — | |
| Mmp9 | E19 | 0.29 ± 0.02 | 0.33 ± 0.03 | 0.32 ± 0.05 | 0.548 | — | — | — |
| E20 | 0.38 ± 0.04 | 0.34 ± 0.05 | 0.34 ± 0.05 | 0.784 | — | — | — | |
| E22 | 1.07 ± 0.10 | 0.87 ± 0.09 | 1.00 ± 0.11 | 0.373 | — | — | — | |
| Timp1 | E19 | 0.82 ± 0.05 | 2.10 ± 0.31 | 0.79 ± 0.06 | <0.001 | — | a | b |
| E20 | 2.79 ± 0.46 | 2.08 ± 0.36 | 2.90 ± 0.34 | 0.349 | — | — | — | |
| E22 | 0.96 ± 0.12 | 1.07 ± 0.17 | 1.00 ± 0.14 | 0.881 | — | — | — | |
| Timp2 | E19 | 0.73 ± 0.07 | 0.87 ± 0.06 | 0.76 ± 0.07 | 0.257 | — | — | — |
| E20 | 0.53 ± 0.05 | 0.60 ± 0.07 | 0.66 ± 0.08 | 0.405 | — | — | — | |
| E22 | 1.10 ± 0.06 | 1.06 ± 0.09 | 1.00 ± 0.10 | 0.745 | — | — | — | |
| Pcna | E19 | 3.90 ± 0.24 | 4.12 ± 0.31 | 3.77 ± 0.28 | 0.709 | — | — | — |
| E20 | 3.59 ± 0.19 | 3.37 ± 0.31 | 2.92 ± 0.30 | 0.237 | — | — | — | |
| E22 | 1.35 ± 0.06 | 1.08 ± 0.11 | 1.00 ± 0.09 | 0.025 | — | — | — |
| Gene . | Day . | LIG . | SOP . | C . | ANOVA . | LIG-C . | LIG-SOP . | SOP-C . |
|---|---|---|---|---|---|---|---|---|
| Tgfb1 | E19 | 1.85 ± 0.10 | 2.00 ± 0.12 | 1.68 ± 0.16 | 0.226 | — | — | — |
| E20 | 1.50 ± 0.11 | 1.34 ± 0.13 | 1.53 ± 0.10 | 0.470 | — | — | — | |
| E22 | 1.22 ± 0.08 | 1.10 ± 0.08 | 1.00 ± 0.09 | 0.142 | — | — | — | |
| Pai1 | E19 | 0.86 ± 0.09 | 1.10 ± 0.13 | 0.24 ± 0.03 | <0.001 | a | — | a |
| E20 | 1.31 ± 0.23 | 0.45 ± 0.08 | 0.26 ± 0.04 | <0.001 | a | b | ns | |
| E22 | 1.29 ± 0.25 | 0.85 ± 0.11 | 1.00 ± 0.17 | 0.599 | — | — | — | |
| IL10 | E19 | 1.23 ± 0.18 | 0.81 ± 0.04 | 0.69 ± 0.08 | 0.009 | c | c | — |
| E20 | 0.84 ± 0.04 | 0.79 ± 0.04 | 0.74 ± 0.04 | 0.236 | — | — | — | |
| E22 | 1.16 ± 0.07 | 1.15 ± 0.07 | 1.00 ± 0.07 | 0.274 | — | — | — | |
| Mmp2 | E19 | 1.08 ± 0.08 | 1.14 ± 0.10 | 0.99 ± 0.09 | 0.558 | — | — | — |
| E20 | 0.46 ± 0.05 | 0.51 ± 0.07 | 0.64 ± 0.09 | 0.167 | — | — | — | |
| E22 | 1.23 ± 0.08 | 1.26 ± 0.07 | 1.00 ± 0.07 | 0.016 | — | — | — | |
| Mmp9 | E19 | 0.29 ± 0.02 | 0.33 ± 0.03 | 0.32 ± 0.05 | 0.548 | — | — | — |
| E20 | 0.38 ± 0.04 | 0.34 ± 0.05 | 0.34 ± 0.05 | 0.784 | — | — | — | |
| E22 | 1.07 ± 0.10 | 0.87 ± 0.09 | 1.00 ± 0.11 | 0.373 | — | — | — | |
| Timp1 | E19 | 0.82 ± 0.05 | 2.10 ± 0.31 | 0.79 ± 0.06 | <0.001 | — | a | b |
| E20 | 2.79 ± 0.46 | 2.08 ± 0.36 | 2.90 ± 0.34 | 0.349 | — | — | — | |
| E22 | 0.96 ± 0.12 | 1.07 ± 0.17 | 1.00 ± 0.14 | 0.881 | — | — | — | |
| Timp2 | E19 | 0.73 ± 0.07 | 0.87 ± 0.06 | 0.76 ± 0.07 | 0.257 | — | — | — |
| E20 | 0.53 ± 0.05 | 0.60 ± 0.07 | 0.66 ± 0.08 | 0.405 | — | — | — | |
| E22 | 1.10 ± 0.06 | 1.06 ± 0.09 | 1.00 ± 0.10 | 0.745 | — | — | — | |
| Pcna | E19 | 3.90 ± 0.24 | 4.12 ± 0.31 | 3.77 ± 0.28 | 0.709 | — | — | — |
| E20 | 3.59 ± 0.19 | 3.37 ± 0.31 | 2.92 ± 0.30 | 0.237 | — | — | — | |
| E22 | 1.35 ± 0.06 | 1.08 ± 0.11 | 1.00 ± 0.09 | 0.025 | — | — | — |
Abbreviation: ns, not significant. For gene abbreviations see Quantitative RT-PCR in Materials and Methods. Data are displayed as relative units (RU). Expression data of each gene were normalized to the E22 mean value of group C. Overall P value of a one-way ANOVA or Kruskal-Wallis-test is indicated. Dashes indicate that a posttest was not done because of a less than 1.5-fold change. Bold font highlights values which were both changed more than 1.5-fold and significantly different compared to the C group.
Significant posttest (P < .001).
Significant posttest (P < .01).
Significant posttest (P < .05).
Expression of Genes Involved in Inflammation and Tissue Remodeling in Fetal Liver of the Groups LIG, SOP, and C on Gestational Days E19, E20, and E22
| Gene . | Day . | LIG . | SOP . | C . | ANOVA . | LIG-C . | LIG-SOP . | SOP-C . |
|---|---|---|---|---|---|---|---|---|
| Tgfb1 | E19 | 1.85 ± 0.10 | 2.00 ± 0.12 | 1.68 ± 0.16 | 0.226 | — | — | — |
| E20 | 1.50 ± 0.11 | 1.34 ± 0.13 | 1.53 ± 0.10 | 0.470 | — | — | — | |
| E22 | 1.22 ± 0.08 | 1.10 ± 0.08 | 1.00 ± 0.09 | 0.142 | — | — | — | |
| Pai1 | E19 | 0.86 ± 0.09 | 1.10 ± 0.13 | 0.24 ± 0.03 | <0.001 | a | — | a |
| E20 | 1.31 ± 0.23 | 0.45 ± 0.08 | 0.26 ± 0.04 | <0.001 | a | b | ns | |
| E22 | 1.29 ± 0.25 | 0.85 ± 0.11 | 1.00 ± 0.17 | 0.599 | — | — | — | |
| IL10 | E19 | 1.23 ± 0.18 | 0.81 ± 0.04 | 0.69 ± 0.08 | 0.009 | c | c | — |
| E20 | 0.84 ± 0.04 | 0.79 ± 0.04 | 0.74 ± 0.04 | 0.236 | — | — | — | |
| E22 | 1.16 ± 0.07 | 1.15 ± 0.07 | 1.00 ± 0.07 | 0.274 | — | — | — | |
| Mmp2 | E19 | 1.08 ± 0.08 | 1.14 ± 0.10 | 0.99 ± 0.09 | 0.558 | — | — | — |
| E20 | 0.46 ± 0.05 | 0.51 ± 0.07 | 0.64 ± 0.09 | 0.167 | — | — | — | |
| E22 | 1.23 ± 0.08 | 1.26 ± 0.07 | 1.00 ± 0.07 | 0.016 | — | — | — | |
| Mmp9 | E19 | 0.29 ± 0.02 | 0.33 ± 0.03 | 0.32 ± 0.05 | 0.548 | — | — | — |
| E20 | 0.38 ± 0.04 | 0.34 ± 0.05 | 0.34 ± 0.05 | 0.784 | — | — | — | |
| E22 | 1.07 ± 0.10 | 0.87 ± 0.09 | 1.00 ± 0.11 | 0.373 | — | — | — | |
| Timp1 | E19 | 0.82 ± 0.05 | 2.10 ± 0.31 | 0.79 ± 0.06 | <0.001 | — | a | b |
| E20 | 2.79 ± 0.46 | 2.08 ± 0.36 | 2.90 ± 0.34 | 0.349 | — | — | — | |
| E22 | 0.96 ± 0.12 | 1.07 ± 0.17 | 1.00 ± 0.14 | 0.881 | — | — | — | |
| Timp2 | E19 | 0.73 ± 0.07 | 0.87 ± 0.06 | 0.76 ± 0.07 | 0.257 | — | — | — |
| E20 | 0.53 ± 0.05 | 0.60 ± 0.07 | 0.66 ± 0.08 | 0.405 | — | — | — | |
| E22 | 1.10 ± 0.06 | 1.06 ± 0.09 | 1.00 ± 0.10 | 0.745 | — | — | — | |
| Pcna | E19 | 3.90 ± 0.24 | 4.12 ± 0.31 | 3.77 ± 0.28 | 0.709 | — | — | — |
| E20 | 3.59 ± 0.19 | 3.37 ± 0.31 | 2.92 ± 0.30 | 0.237 | — | — | — | |
| E22 | 1.35 ± 0.06 | 1.08 ± 0.11 | 1.00 ± 0.09 | 0.025 | — | — | — |
| Gene . | Day . | LIG . | SOP . | C . | ANOVA . | LIG-C . | LIG-SOP . | SOP-C . |
|---|---|---|---|---|---|---|---|---|
| Tgfb1 | E19 | 1.85 ± 0.10 | 2.00 ± 0.12 | 1.68 ± 0.16 | 0.226 | — | — | — |
| E20 | 1.50 ± 0.11 | 1.34 ± 0.13 | 1.53 ± 0.10 | 0.470 | — | — | — | |
| E22 | 1.22 ± 0.08 | 1.10 ± 0.08 | 1.00 ± 0.09 | 0.142 | — | — | — | |
| Pai1 | E19 | 0.86 ± 0.09 | 1.10 ± 0.13 | 0.24 ± 0.03 | <0.001 | a | — | a |
| E20 | 1.31 ± 0.23 | 0.45 ± 0.08 | 0.26 ± 0.04 | <0.001 | a | b | ns | |
| E22 | 1.29 ± 0.25 | 0.85 ± 0.11 | 1.00 ± 0.17 | 0.599 | — | — | — | |
| IL10 | E19 | 1.23 ± 0.18 | 0.81 ± 0.04 | 0.69 ± 0.08 | 0.009 | c | c | — |
| E20 | 0.84 ± 0.04 | 0.79 ± 0.04 | 0.74 ± 0.04 | 0.236 | — | — | — | |
| E22 | 1.16 ± 0.07 | 1.15 ± 0.07 | 1.00 ± 0.07 | 0.274 | — | — | — | |
| Mmp2 | E19 | 1.08 ± 0.08 | 1.14 ± 0.10 | 0.99 ± 0.09 | 0.558 | — | — | — |
| E20 | 0.46 ± 0.05 | 0.51 ± 0.07 | 0.64 ± 0.09 | 0.167 | — | — | — | |
| E22 | 1.23 ± 0.08 | 1.26 ± 0.07 | 1.00 ± 0.07 | 0.016 | — | — | — | |
| Mmp9 | E19 | 0.29 ± 0.02 | 0.33 ± 0.03 | 0.32 ± 0.05 | 0.548 | — | — | — |
| E20 | 0.38 ± 0.04 | 0.34 ± 0.05 | 0.34 ± 0.05 | 0.784 | — | — | — | |
| E22 | 1.07 ± 0.10 | 0.87 ± 0.09 | 1.00 ± 0.11 | 0.373 | — | — | — | |
| Timp1 | E19 | 0.82 ± 0.05 | 2.10 ± 0.31 | 0.79 ± 0.06 | <0.001 | — | a | b |
| E20 | 2.79 ± 0.46 | 2.08 ± 0.36 | 2.90 ± 0.34 | 0.349 | — | — | — | |
| E22 | 0.96 ± 0.12 | 1.07 ± 0.17 | 1.00 ± 0.14 | 0.881 | — | — | — | |
| Timp2 | E19 | 0.73 ± 0.07 | 0.87 ± 0.06 | 0.76 ± 0.07 | 0.257 | — | — | — |
| E20 | 0.53 ± 0.05 | 0.60 ± 0.07 | 0.66 ± 0.08 | 0.405 | — | — | — | |
| E22 | 1.10 ± 0.06 | 1.06 ± 0.09 | 1.00 ± 0.10 | 0.745 | — | — | — | |
| Pcna | E19 | 3.90 ± 0.24 | 4.12 ± 0.31 | 3.77 ± 0.28 | 0.709 | — | — | — |
| E20 | 3.59 ± 0.19 | 3.37 ± 0.31 | 2.92 ± 0.30 | 0.237 | — | — | — | |
| E22 | 1.35 ± 0.06 | 1.08 ± 0.11 | 1.00 ± 0.09 | 0.025 | — | — | — |
Abbreviation: ns, not significant. For gene abbreviations see Quantitative RT-PCR in Materials and Methods. Data are displayed as relative units (RU). Expression data of each gene were normalized to the E22 mean value of group C. Overall P value of a one-way ANOVA or Kruskal-Wallis-test is indicated. Dashes indicate that a posttest was not done because of a less than 1.5-fold change. Bold font highlights values which were both changed more than 1.5-fold and significantly different compared to the C group.
Significant posttest (P < .001).
Significant posttest (P < .01).
Significant posttest (P < .05).
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