Table 2.
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Primers used for genotyping assays

GeneForward primer(s)Reverse primer(s)
Hsd17b3Hsd17b3 forward:
ggagaagctcttcattgctg		Hsd17b3 WT-reverse:
cttacctgcccattgtcccat
Hsd17b3 del-reverse:
cttacctgtcccattgatcg
Hsd17b12Hsd17b12 WT-forward:
acattagaaacctcacgctgct		Hsd17b12 WT-reverse:
ttgccagttttgtagctacaagg
Hsd17b12 mut-forward:
acattagaaacctcacgctgct		Hsd17b12 Mut-forward:
cagttttgtcgcgacgaa
Interleukin-2 (Il2) (Positive control gene)Il2 forward:
ctaggccacagaattgaaagatct		Il2 reverse:
gtaggtggaaattctagcatcatcc
GeneForward primer(s)Reverse primer(s)
Hsd17b3Hsd17b3 forward:
ggagaagctcttcattgctg		Hsd17b3 WT-reverse:
cttacctgcccattgtcccat
Hsd17b3 del-reverse:
cttacctgtcccattgatcg
Hsd17b12Hsd17b12 WT-forward:
acattagaaacctcacgctgct		Hsd17b12 WT-reverse:
ttgccagttttgtagctacaagg
Hsd17b12 mut-forward:
acattagaaacctcacgctgct		Hsd17b12 Mut-forward:
cagttttgtcgcgacgaa
Interleukin-2 (Il2) (Positive control gene)Il2 forward:
ctaggccacagaattgaaagatct		Il2 reverse:
gtaggtggaaattctagcatcatcc
Table 2.
Open in new tab

Primers used for genotyping assays

GeneForward primer(s)Reverse primer(s)
Hsd17b3Hsd17b3 forward:
ggagaagctcttcattgctg		Hsd17b3 WT-reverse:
cttacctgcccattgtcccat
Hsd17b3 del-reverse:
cttacctgtcccattgatcg
Hsd17b12Hsd17b12 WT-forward:
acattagaaacctcacgctgct		Hsd17b12 WT-reverse:
ttgccagttttgtagctacaagg
Hsd17b12 mut-forward:
acattagaaacctcacgctgct		Hsd17b12 Mut-forward:
cagttttgtcgcgacgaa
Interleukin-2 (Il2) (Positive control gene)Il2 forward:
ctaggccacagaattgaaagatct		Il2 reverse:
gtaggtggaaattctagcatcatcc
GeneForward primer(s)Reverse primer(s)
Hsd17b3Hsd17b3 forward:
ggagaagctcttcattgctg		Hsd17b3 WT-reverse:
cttacctgcccattgtcccat
Hsd17b3 del-reverse:
cttacctgtcccattgatcg
Hsd17b12Hsd17b12 WT-forward:
acattagaaacctcacgctgct		Hsd17b12 WT-reverse:
ttgccagttttgtagctacaagg
Hsd17b12 mut-forward:
acattagaaacctcacgctgct		Hsd17b12 Mut-forward:
cagttttgtcgcgacgaa
Interleukin-2 (Il2) (Positive control gene)Il2 forward:
ctaggccacagaattgaaagatct		Il2 reverse:
gtaggtggaaattctagcatcatcc
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