Table 1.
Open in new tab

Antibodies Used for Immunoblotting and ChIP Assaysa

PurposeAntibodySource (Catalog No.), Dilution
ImmunoblotsActinSanta Cruz (sc-47778), 1:500
DNMT1Upstate (07-688), 1:500
DNMT3AAbcam (ab23565), 1:200
EHMT2Cell Signaling (3306), 1:1000
EZH2Active Motif (39933), 1:1000
HDAC1Santa Cruz (sc-7872), 1:1000
HDAC2Santa Cruz (sc-7899), 1:1000
HDAC3Santa Cruz (sc-11417), 1:1000
KAT2ASanta Cruz (sc-20698), 1:500
Lamin A/CSanta Cruz (sc-20681), 1:1000
SETDB1bUpstate (07-378), 1:500
SUZ12Upstate (07-379), 1:1000
TET1GeneTex (GTX124207), 1:1000
ChIP/histone immunoblotsaHistone H3K4me3Active Motif (39159)
Histone H3K9me2Millipore (07-441)
Histone H3K27me3Active Motif (39155)
Histone H3 acetylMillipore (04-928)
Histone H4 acetylMillipore (06-866)
Histone H3K9 acetylMillipore (07-352)
Histone H4K5 acetylActive Motif (39583)
Normal rabbit serumSanta Cruz (sc-2027)
SecondaryPeroxidase antimouseJackson ImmunoResearch (115-035-174)
Peroxidase antirabbitJackson ImmunoResearch (711-035-152)
PurposeAntibodySource (Catalog No.), Dilution
ImmunoblotsActinSanta Cruz (sc-47778), 1:500
DNMT1Upstate (07-688), 1:500
DNMT3AAbcam (ab23565), 1:200
EHMT2Cell Signaling (3306), 1:1000
EZH2Active Motif (39933), 1:1000
HDAC1Santa Cruz (sc-7872), 1:1000
HDAC2Santa Cruz (sc-7899), 1:1000
HDAC3Santa Cruz (sc-11417), 1:1000
KAT2ASanta Cruz (sc-20698), 1:500
Lamin A/CSanta Cruz (sc-20681), 1:1000
SETDB1bUpstate (07-378), 1:500
SUZ12Upstate (07-379), 1:1000
TET1GeneTex (GTX124207), 1:1000
ChIP/histone immunoblotsaHistone H3K4me3Active Motif (39159)
Histone H3K9me2Millipore (07-441)
Histone H3K27me3Active Motif (39155)
Histone H3 acetylMillipore (04-928)
Histone H4 acetylMillipore (06-866)
Histone H3K9 acetylMillipore (07-352)
Histone H4K5 acetylActive Motif (39583)
Normal rabbit serumSanta Cruz (sc-2027)
SecondaryPeroxidase antimouseJackson ImmunoResearch (115-035-174)
Peroxidase antirabbitJackson ImmunoResearch (711-035-152)
a

All ChIP/histone antibodies were used at 5 μg/sample for ChIP and 1:1000 for immunoblots.

b

SETDB1, SET domain protein, bifurcated 1.

Table 1.
Open in new tab

Antibodies Used for Immunoblotting and ChIP Assaysa

PurposeAntibodySource (Catalog No.), Dilution
ImmunoblotsActinSanta Cruz (sc-47778), 1:500
DNMT1Upstate (07-688), 1:500
DNMT3AAbcam (ab23565), 1:200
EHMT2Cell Signaling (3306), 1:1000
EZH2Active Motif (39933), 1:1000
HDAC1Santa Cruz (sc-7872), 1:1000
HDAC2Santa Cruz (sc-7899), 1:1000
HDAC3Santa Cruz (sc-11417), 1:1000
KAT2ASanta Cruz (sc-20698), 1:500
Lamin A/CSanta Cruz (sc-20681), 1:1000
SETDB1bUpstate (07-378), 1:500
SUZ12Upstate (07-379), 1:1000
TET1GeneTex (GTX124207), 1:1000
ChIP/histone immunoblotsaHistone H3K4me3Active Motif (39159)
Histone H3K9me2Millipore (07-441)
Histone H3K27me3Active Motif (39155)
Histone H3 acetylMillipore (04-928)
Histone H4 acetylMillipore (06-866)
Histone H3K9 acetylMillipore (07-352)
Histone H4K5 acetylActive Motif (39583)
Normal rabbit serumSanta Cruz (sc-2027)
SecondaryPeroxidase antimouseJackson ImmunoResearch (115-035-174)
Peroxidase antirabbitJackson ImmunoResearch (711-035-152)
PurposeAntibodySource (Catalog No.), Dilution
ImmunoblotsActinSanta Cruz (sc-47778), 1:500
DNMT1Upstate (07-688), 1:500
DNMT3AAbcam (ab23565), 1:200
EHMT2Cell Signaling (3306), 1:1000
EZH2Active Motif (39933), 1:1000
HDAC1Santa Cruz (sc-7872), 1:1000
HDAC2Santa Cruz (sc-7899), 1:1000
HDAC3Santa Cruz (sc-11417), 1:1000
KAT2ASanta Cruz (sc-20698), 1:500
Lamin A/CSanta Cruz (sc-20681), 1:1000
SETDB1bUpstate (07-378), 1:500
SUZ12Upstate (07-379), 1:1000
TET1GeneTex (GTX124207), 1:1000
ChIP/histone immunoblotsaHistone H3K4me3Active Motif (39159)
Histone H3K9me2Millipore (07-441)
Histone H3K27me3Active Motif (39155)
Histone H3 acetylMillipore (04-928)
Histone H4 acetylMillipore (06-866)
Histone H3K9 acetylMillipore (07-352)
Histone H4K5 acetylActive Motif (39583)
Normal rabbit serumSanta Cruz (sc-2027)
SecondaryPeroxidase antimouseJackson ImmunoResearch (115-035-174)
Peroxidase antirabbitJackson ImmunoResearch (711-035-152)
a

All ChIP/histone antibodies were used at 5 μg/sample for ChIP and 1:1000 for immunoblots.

b

SETDB1, SET domain protein, bifurcated 1.

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