Change of LF secondary structure content in binary and ternary systems after adding AA/EPA and RU.
| System . | β-Sheet (%) . | Random coil (%) . | α-Helix (%) . | β-Turn (%) . | Antiparallel β-sheet (%) . |
|---|---|---|---|---|---|
| LF | 24.3482 ± 0.0772a | 17.506 ± 0.0608d | 18.6359 ± 0.0066c | 28.0471 ± 0.137e | 11.4606 ± 0.0052a |
| AA-LF | 23.3316 ± 0.0040d | 18.1073 ± 0.0065b | 19.6814 ± 0.0054b | 28.3241 ± 0.0080c | 10.5555 ± 0.0159c |
| EPA-LF | 23.4781 ± 0.0448c | 18.2012 ± 0.0196a | 19.7648 ± 0.0034b | 28.2451 ± 0.0072cd | 10.3107 ± 0.0214d |
| RU-LF | 23.2391 ± 0.0247d | 17.8746 ± 0.0130c | 20.0627 ± 0.0039a | 28.1123 ± 0.0878de | 10.7114 ± 0.0103b |
| RU-AA-LF | 23.9658 ± 0.0711b | 15.2511 ± 0.0097e | 15.8879 ± 0.0705e | 34.957 ± 0.0037b | 9.9381 ± 0.0054e |
| RU-EPA-LF | 21.6913 ± 0.0394e | 14.8432 ± 0.0103f | 16.6556 ± 0.0456d | 36.1336 ± 0.0434a | 10.6762 ± 0.0393b |
| System . | β-Sheet (%) . | Random coil (%) . | α-Helix (%) . | β-Turn (%) . | Antiparallel β-sheet (%) . |
|---|---|---|---|---|---|
| LF | 24.3482 ± 0.0772a | 17.506 ± 0.0608d | 18.6359 ± 0.0066c | 28.0471 ± 0.137e | 11.4606 ± 0.0052a |
| AA-LF | 23.3316 ± 0.0040d | 18.1073 ± 0.0065b | 19.6814 ± 0.0054b | 28.3241 ± 0.0080c | 10.5555 ± 0.0159c |
| EPA-LF | 23.4781 ± 0.0448c | 18.2012 ± 0.0196a | 19.7648 ± 0.0034b | 28.2451 ± 0.0072cd | 10.3107 ± 0.0214d |
| RU-LF | 23.2391 ± 0.0247d | 17.8746 ± 0.0130c | 20.0627 ± 0.0039a | 28.1123 ± 0.0878de | 10.7114 ± 0.0103b |
| RU-AA-LF | 23.9658 ± 0.0711b | 15.2511 ± 0.0097e | 15.8879 ± 0.0705e | 34.957 ± 0.0037b | 9.9381 ± 0.0054e |
| RU-EPA-LF | 21.6913 ± 0.0394e | 14.8432 ± 0.0103f | 16.6556 ± 0.0456d | 36.1336 ± 0.0434a | 10.6762 ± 0.0393b |
Note. AA = arachidonic acid; EPA = eicosapentaenoic acid; LF = lactoferrin; RU = rutin. Means with different letters (a–f) differ significantly (P < 0.05).
Change of LF secondary structure content in binary and ternary systems after adding AA/EPA and RU.
| System . | β-Sheet (%) . | Random coil (%) . | α-Helix (%) . | β-Turn (%) . | Antiparallel β-sheet (%) . |
|---|---|---|---|---|---|
| LF | 24.3482 ± 0.0772a | 17.506 ± 0.0608d | 18.6359 ± 0.0066c | 28.0471 ± 0.137e | 11.4606 ± 0.0052a |
| AA-LF | 23.3316 ± 0.0040d | 18.1073 ± 0.0065b | 19.6814 ± 0.0054b | 28.3241 ± 0.0080c | 10.5555 ± 0.0159c |
| EPA-LF | 23.4781 ± 0.0448c | 18.2012 ± 0.0196a | 19.7648 ± 0.0034b | 28.2451 ± 0.0072cd | 10.3107 ± 0.0214d |
| RU-LF | 23.2391 ± 0.0247d | 17.8746 ± 0.0130c | 20.0627 ± 0.0039a | 28.1123 ± 0.0878de | 10.7114 ± 0.0103b |
| RU-AA-LF | 23.9658 ± 0.0711b | 15.2511 ± 0.0097e | 15.8879 ± 0.0705e | 34.957 ± 0.0037b | 9.9381 ± 0.0054e |
| RU-EPA-LF | 21.6913 ± 0.0394e | 14.8432 ± 0.0103f | 16.6556 ± 0.0456d | 36.1336 ± 0.0434a | 10.6762 ± 0.0393b |
| System . | β-Sheet (%) . | Random coil (%) . | α-Helix (%) . | β-Turn (%) . | Antiparallel β-sheet (%) . |
|---|---|---|---|---|---|
| LF | 24.3482 ± 0.0772a | 17.506 ± 0.0608d | 18.6359 ± 0.0066c | 28.0471 ± 0.137e | 11.4606 ± 0.0052a |
| AA-LF | 23.3316 ± 0.0040d | 18.1073 ± 0.0065b | 19.6814 ± 0.0054b | 28.3241 ± 0.0080c | 10.5555 ± 0.0159c |
| EPA-LF | 23.4781 ± 0.0448c | 18.2012 ± 0.0196a | 19.7648 ± 0.0034b | 28.2451 ± 0.0072cd | 10.3107 ± 0.0214d |
| RU-LF | 23.2391 ± 0.0247d | 17.8746 ± 0.0130c | 20.0627 ± 0.0039a | 28.1123 ± 0.0878de | 10.7114 ± 0.0103b |
| RU-AA-LF | 23.9658 ± 0.0711b | 15.2511 ± 0.0097e | 15.8879 ± 0.0705e | 34.957 ± 0.0037b | 9.9381 ± 0.0054e |
| RU-EPA-LF | 21.6913 ± 0.0394e | 14.8432 ± 0.0103f | 16.6556 ± 0.0456d | 36.1336 ± 0.0434a | 10.6762 ± 0.0393b |
Note. AA = arachidonic acid; EPA = eicosapentaenoic acid; LF = lactoferrin; RU = rutin. Means with different letters (a–f) differ significantly (P < 0.05).
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