Effect of supplementing PGCs with PL, AS-3, and HEPES on granulocyte and monocyte functions during room-temperature storage.
| Diluent . | Cells type . | Viability (%) . | Phagocytosis (MFI) . | Intracellular ROS (MFI) . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | ||
| None | Granulocyte | 70.5 (5.4) | 59.5 (5.1) | 40.4 (7.8) | 43.0 (4.8) | 23,644 (1,111) | 34,283 (3,624) | 30,428 (3,731) | 25,316 (3,591) | 1.23 (0.19) | 1.43 (0.22) | 1.33 (0.22) | 1.47 (0.13) |
| Monocyte | 50.1 (8.2) | 24.7 (6.9) | 13.5 (2.3)a | 22.5 (4.0) | 20,645 (1,318) | 28,060 (3,909) | 25,261 (3,934) | 18,186 (2,917) | 1.17 (0.03) | 1.10 (0.06) | 1.17 (0.15) | 1.27 (0.09) | |
| AS-3⊕ | Granulocyte | … | … | 37.9 (7.0) | 35.4 (3.3)a | … | … | 26,887 (1,966) | 19,909 (3,004)b | … | … | 1.50 (0.06) | 1.37 (0.15) |
| Monocyte | … | … | 15.0 (4.2)a | 21.4 (3.1) | … | … | 20,883 (5,116) | 11,716 (1,450)b | … | … | 1.30 (0.06) | 1.13 (0.09) | |
| AS-3⊕ + 2.5% HEPES | Granulocyte | … | … | 48.1 (7.6) | 44.5 (2.6) | … | … | 27,249 (2,273) | 24,543 (3,767) | … | … | 1.60 (0.12) | 1.57 (0.19) |
| Monocyte | … | … | 19.5 (4.8) | 23.3 (1.5) | … | … | 22,624 (4,708) | 15,012 (1,999) | … | … | 1.30 (0.06) | 1.33 (0.18) | |
| AS-3⊕ + 7.5% HEPES | Granulocyte | … | … | 43.0 (6.1) | 41.6 (4.0)a | … | … | 25,457 (1,614) | 20,503 (3,455)b | … | … | 1.60 (0.10) | 1.60 (0.15) |
| Monocyte | … | … | 14.3 (3.3)a | 21.7 (3.2) | … | … | 15,372 (2,130)b | 12,015 (1,770)b | … | … | 1.43 (0.12) | 1.40 (0.10) | |
| Diluent . | Cells type . | Viability (%) . | Phagocytosis (MFI) . | Intracellular ROS (MFI) . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | ||
| None | Granulocyte | 70.5 (5.4) | 59.5 (5.1) | 40.4 (7.8) | 43.0 (4.8) | 23,644 (1,111) | 34,283 (3,624) | 30,428 (3,731) | 25,316 (3,591) | 1.23 (0.19) | 1.43 (0.22) | 1.33 (0.22) | 1.47 (0.13) |
| Monocyte | 50.1 (8.2) | 24.7 (6.9) | 13.5 (2.3)a | 22.5 (4.0) | 20,645 (1,318) | 28,060 (3,909) | 25,261 (3,934) | 18,186 (2,917) | 1.17 (0.03) | 1.10 (0.06) | 1.17 (0.15) | 1.27 (0.09) | |
| AS-3⊕ | Granulocyte | … | … | 37.9 (7.0) | 35.4 (3.3)a | … | … | 26,887 (1,966) | 19,909 (3,004)b | … | … | 1.50 (0.06) | 1.37 (0.15) |
| Monocyte | … | … | 15.0 (4.2)a | 21.4 (3.1) | … | … | 20,883 (5,116) | 11,716 (1,450)b | … | … | 1.30 (0.06) | 1.13 (0.09) | |
| AS-3⊕ + 2.5% HEPES | Granulocyte | … | … | 48.1 (7.6) | 44.5 (2.6) | … | … | 27,249 (2,273) | 24,543 (3,767) | … | … | 1.60 (0.12) | 1.57 (0.19) |
| Monocyte | … | … | 19.5 (4.8) | 23.3 (1.5) | … | … | 22,624 (4,708) | 15,012 (1,999) | … | … | 1.30 (0.06) | 1.33 (0.18) | |
| AS-3⊕ + 7.5% HEPES | Granulocyte | … | … | 43.0 (6.1) | 41.6 (4.0)a | … | … | 25,457 (1,614) | 20,503 (3,455)b | … | … | 1.60 (0.10) | 1.60 (0.15) |
| Monocyte | … | … | 14.3 (3.3)a | 21.7 (3.2) | … | … | 15,372 (2,130)b | 12,015 (1,770)b | … | … | 1.43 (0.12) | 1.40 (0.10) | |
AS-3⊕ = AS-3 + Plasma-Lyte + Alburex.
Data are expressed as mean (SEM).
aP < 0.05 represents the comparison to values at 0 h in whole blood.
bP < 0.05 represents the comparison to values at 16 to 24 h without diluent.
N = 3 to 4 pool of 5 donors.
Effect of supplementing PGCs with PL, AS-3, and HEPES on granulocyte and monocyte functions during room-temperature storage.
| Diluent . | Cells type . | Viability (%) . | Phagocytosis (MFI) . | Intracellular ROS (MFI) . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | ||
| None | Granulocyte | 70.5 (5.4) | 59.5 (5.1) | 40.4 (7.8) | 43.0 (4.8) | 23,644 (1,111) | 34,283 (3,624) | 30,428 (3,731) | 25,316 (3,591) | 1.23 (0.19) | 1.43 (0.22) | 1.33 (0.22) | 1.47 (0.13) |
| Monocyte | 50.1 (8.2) | 24.7 (6.9) | 13.5 (2.3)a | 22.5 (4.0) | 20,645 (1,318) | 28,060 (3,909) | 25,261 (3,934) | 18,186 (2,917) | 1.17 (0.03) | 1.10 (0.06) | 1.17 (0.15) | 1.27 (0.09) | |
| AS-3⊕ | Granulocyte | … | … | 37.9 (7.0) | 35.4 (3.3)a | … | … | 26,887 (1,966) | 19,909 (3,004)b | … | … | 1.50 (0.06) | 1.37 (0.15) |
| Monocyte | … | … | 15.0 (4.2)a | 21.4 (3.1) | … | … | 20,883 (5,116) | 11,716 (1,450)b | … | … | 1.30 (0.06) | 1.13 (0.09) | |
| AS-3⊕ + 2.5% HEPES | Granulocyte | … | … | 48.1 (7.6) | 44.5 (2.6) | … | … | 27,249 (2,273) | 24,543 (3,767) | … | … | 1.60 (0.12) | 1.57 (0.19) |
| Monocyte | … | … | 19.5 (4.8) | 23.3 (1.5) | … | … | 22,624 (4,708) | 15,012 (1,999) | … | … | 1.30 (0.06) | 1.33 (0.18) | |
| AS-3⊕ + 7.5% HEPES | Granulocyte | … | … | 43.0 (6.1) | 41.6 (4.0)a | … | … | 25,457 (1,614) | 20,503 (3,455)b | … | … | 1.60 (0.10) | 1.60 (0.15) |
| Monocyte | … | … | 14.3 (3.3)a | 21.7 (3.2) | … | … | 15,372 (2,130)b | 12,015 (1,770)b | … | … | 1.43 (0.12) | 1.40 (0.10) | |
| Diluent . | Cells type . | Viability (%) . | Phagocytosis (MFI) . | Intracellular ROS (MFI) . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | 0 h . | 16 to 24 h . | 40 to 48 h . | 64 to 72 h . | ||
| None | Granulocyte | 70.5 (5.4) | 59.5 (5.1) | 40.4 (7.8) | 43.0 (4.8) | 23,644 (1,111) | 34,283 (3,624) | 30,428 (3,731) | 25,316 (3,591) | 1.23 (0.19) | 1.43 (0.22) | 1.33 (0.22) | 1.47 (0.13) |
| Monocyte | 50.1 (8.2) | 24.7 (6.9) | 13.5 (2.3)a | 22.5 (4.0) | 20,645 (1,318) | 28,060 (3,909) | 25,261 (3,934) | 18,186 (2,917) | 1.17 (0.03) | 1.10 (0.06) | 1.17 (0.15) | 1.27 (0.09) | |
| AS-3⊕ | Granulocyte | … | … | 37.9 (7.0) | 35.4 (3.3)a | … | … | 26,887 (1,966) | 19,909 (3,004)b | … | … | 1.50 (0.06) | 1.37 (0.15) |
| Monocyte | … | … | 15.0 (4.2)a | 21.4 (3.1) | … | … | 20,883 (5,116) | 11,716 (1,450)b | … | … | 1.30 (0.06) | 1.13 (0.09) | |
| AS-3⊕ + 2.5% HEPES | Granulocyte | … | … | 48.1 (7.6) | 44.5 (2.6) | … | … | 27,249 (2,273) | 24,543 (3,767) | … | … | 1.60 (0.12) | 1.57 (0.19) |
| Monocyte | … | … | 19.5 (4.8) | 23.3 (1.5) | … | … | 22,624 (4,708) | 15,012 (1,999) | … | … | 1.30 (0.06) | 1.33 (0.18) | |
| AS-3⊕ + 7.5% HEPES | Granulocyte | … | … | 43.0 (6.1) | 41.6 (4.0)a | … | … | 25,457 (1,614) | 20,503 (3,455)b | … | … | 1.60 (0.10) | 1.60 (0.15) |
| Monocyte | … | … | 14.3 (3.3)a | 21.7 (3.2) | … | … | 15,372 (2,130)b | 12,015 (1,770)b | … | … | 1.43 (0.12) | 1.40 (0.10) | |
AS-3⊕ = AS-3 + Plasma-Lyte + Alburex.
Data are expressed as mean (SEM).
aP < 0.05 represents the comparison to values at 0 h in whole blood.
bP < 0.05 represents the comparison to values at 16 to 24 h without diluent.
N = 3 to 4 pool of 5 donors.
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