Table 2. Open in new tab Summary results...

Compound (CAS)	Method	S9 Species (induction)	Vehicle	Strain S typhimurium or E coli^*	Result
Methyl(neopentyl)nitrosamine (CAS 31820-22-1)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	+ive	Positive
None³	Methanol	NT	NT	NT	NT	−ive	Negative
None⁴	Methanol	NT	NT	NT	NT	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
Bis(2-methoxyethyl)nitrosoamine (CAS 67856-65-9)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
N-Nitroso-N-methyl-4-fluoroaniline (CAS 937-25-7)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	+ive	−ive	−ive	−ive	+ive	Positive
Hamster	Methanol	+ive	−ive	−ive	−ive	−ive	Positive
Dinitrosoethambutol, (R,R)-(CAS 52322-22-2)¹	Plate	None	Methanol	−ive	−ive	−ive	+ive	−ive	Positive
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	−ive	−ive	+ive	−ive	Positive
N-methyl-N-nitroso-2-propanamine (CAS 30533-08-5)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
N-Nitrosodiisopropylamine (CAS 601-77-4)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
1-Cyclopentyl-4-nitrosopiperazine (CAS 61379-66-6)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat (10%)	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
Hamster (10%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
Rat (30%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
Hamster (30%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
Mononitrosocaffeidine (CAS 145438-96-6, Batch: 170457)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Hamster	Methanol	−ive	−ive	−ive	−ive	−ive	Negative

Compound (CAS)

Method

S9 Species (induction)

Vehicle

Strain S typhimurium or E coli^*

Result

TA98

TA100

TA1537

TA1535

WP2^*

Methyl(neopentyl)nitrosamine (CAS 31820-22-1)²

Plate

None

Methanol

−ive

+ive

Positive

None³

Methanol

−ive

Negative

None⁴

Methanol

−ive

Negative

Pre-Inc

Rat

Methanol

−ive

Negative

Hamster

Methanol

−ive

+ive

−ive

+ive

−ive

Positive

Bis(2-methoxyethyl)nitrosoamine (CAS 67856-65-9)¹

Plate

None

Methanol

−ive

Negative

Pre-Inc

Rat

Methanol

−ive

Negative

Hamster

Methanol

−ive

+ive

−ive

+ive

−ive

Positive

N-Nitroso-N-methyl-4-fluoroaniline (CAS 937-25-7)²

Plate

None

Methanol

−ive

Negative

Pre-Inc

Rat

Methanol

+ive

−ive

+ive

Positive

Hamster

Methanol

+ive

−ive

Positive

Dinitrosoethambutol, (R,R)-(CAS 52322-22-2)¹

Plate

None

Methanol

−ive

+ive

−ive

Positive

Pre-Inc

Rat

Methanol

−ive

Negative

Hamster

Methanol

−ive

+ive

−ive

Positive

N-methyl-N-nitroso-2-propanamine (CAS 30533-08-5)¹

Plate

None

Methanol

−ive

Negative

Pre-Inc

Rat

Methanol

−ive

Negative

Hamster

Methanol

−ive

+ive

−ive

+ive

−ive

Positive

N-Nitrosodiisopropylamine (CAS 601-77-4)²

Plate

None

Methanol

−ive

Negative

Pre-Inc

Rat

Methanol

−ive

Negative

Hamster

Methanol

−ive

Negative

1-Cyclopentyl-4-nitrosopiperazine (CAS 61379-66-6)¹

Plate

None

Methanol

−ive

Negative

Pre-Inc

Rat (10%)

Methanol

−ive

+ive

−ive

+ive

−ive

Positive

Hamster (10%)

Methanol

−ive

+ive

−ive

+ive

Positive

Rat (30%)

Methanol

−ive

+ive

−ive

+ive

Positive

Hamster (30%)

Methanol

−ive

+ive

−ive

+ive

Positive

Mononitrosocaffeidine (CAS 145438-96-6, Batch: 170457)¹

Plate

None

Methanol

−ive

Negative

Pre-Inc

Rat

Methanol

−ive

Negative

Hamster

Methanol

−ive

Negative

Compound (CAS)	Method	S9 Species (induction)	Vehicle	Strain S typhimurium or E coli^*	Result
Methyl(neopentyl)nitrosamine (CAS 31820-22-1)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	+ive	Positive
None³	Methanol	NT	NT	NT	NT	−ive	Negative
None⁴	Methanol	NT	NT	NT	NT	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
Bis(2-methoxyethyl)nitrosoamine (CAS 67856-65-9)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
N-Nitroso-N-methyl-4-fluoroaniline (CAS 937-25-7)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	+ive	−ive	−ive	−ive	+ive	Positive
Hamster	Methanol	+ive	−ive	−ive	−ive	−ive	Positive
Dinitrosoethambutol, (R,R)-(CAS 52322-22-2)¹	Plate	None	Methanol	−ive	−ive	−ive	+ive	−ive	Positive
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	−ive	−ive	+ive	−ive	Positive
N-methyl-N-nitroso-2-propanamine (CAS 30533-08-5)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
N-Nitrosodiisopropylamine (CAS 601-77-4)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Hamster	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
1-Cyclopentyl-4-nitrosopiperazine (CAS 61379-66-6)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat (10%)	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
Hamster (10%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
Rat (30%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
Hamster (30%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
Mononitrosocaffeidine (CAS 145438-96-6, Batch: 170457)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Hamster	Methanol	−ive	−ive	−ive	−ive	−ive	Negative

Table 2.

Open in new tab

Summary results of historically discordant nitrosamines tested (methanol).

Compound (CAS)	Method	S9 Species (induction)	Vehicle	Strain S typhimurium or E coli^*					Result
Compound (CAS)	Method	S9 Species (induction)	Vehicle	TA98	TA100	TA1537	TA1535	WP2^*	Result
Methyl(neopentyl)nitrosamine (CAS 31820-22-1)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	+ive	Positive
		None³	Methanol	NT	NT	NT	NT	−ive	Negative
		None⁴	Methanol	NT	NT	NT	NT	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
Bis(2-methoxyethyl)nitrosoamine (CAS 67856-65-9)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
N-Nitroso-N-methyl-4-fluoroaniline (CAS 937-25-7)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	+ive	−ive	−ive	−ive	+ive	Positive
	Pre-Inc	Hamster	Methanol	+ive	−ive	−ive	−ive	−ive	Positive
Dinitrosoethambutol, (R,R)-(CAS 52322-22-2)¹	Plate	None	Methanol	−ive	−ive	−ive	+ive	−ive	Positive
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	−ive	−ive	+ive	−ive	Positive
N-methyl-N-nitroso-2-propanamine (CAS 30533-08-5)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
N-Nitrosodiisopropylamine (CAS 601-77-4)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
1-Cyclopentyl-4-nitrosopiperazine (CAS 61379-66-6)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat (10%)	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
		Hamster (10%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
		Rat (30%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
		Hamster (30%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
Mononitrosocaffeidine (CAS 145438-96-6, Batch: 170457)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
		Hamster	Methanol	−ive	−ive	−ive	−ive	−ive	Negative

Compound (CAS)	Method	S9 Species (induction)	Vehicle	Strain S typhimurium or E coli^*					Result
Compound (CAS)	Method	S9 Species (induction)	Vehicle	TA98	TA100	TA1537	TA1535	WP2^*	Result
Methyl(neopentyl)nitrosamine (CAS 31820-22-1)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	+ive	Positive
		None³	Methanol	NT	NT	NT	NT	−ive	Negative
		None⁴	Methanol	NT	NT	NT	NT	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
Bis(2-methoxyethyl)nitrosoamine (CAS 67856-65-9)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
N-Nitroso-N-methyl-4-fluoroaniline (CAS 937-25-7)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	+ive	−ive	−ive	−ive	+ive	Positive
	Pre-Inc	Hamster	Methanol	+ive	−ive	−ive	−ive	−ive	Positive
Dinitrosoethambutol, (R,R)-(CAS 52322-22-2)¹	Plate	None	Methanol	−ive	−ive	−ive	+ive	−ive	Positive
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	−ive	−ive	+ive	−ive	Positive
N-methyl-N-nitroso-2-propanamine (CAS 30533-08-5)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
N-Nitrosodiisopropylamine (CAS 601-77-4)²	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Hamster	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
1-Cyclopentyl-4-nitrosopiperazine (CAS 61379-66-6)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat (10%)	Methanol	−ive	+ive	−ive	+ive	−ive	Positive
		Hamster (10%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
		Rat (30%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
		Hamster (30%)	Methanol	−ive	+ive	−ive	+ive	+ive	Positive
Mononitrosocaffeidine (CAS 145438-96-6, Batch: 170457)¹	Plate	None	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
	Pre-Inc	Rat	Methanol	−ive	−ive	−ive	−ive	−ive	Negative
		Hamster	Methanol	−ive	−ive	−ive	−ive	−ive	Negative

¹The maximum concentration tested was 5000 μg/plate, the maximum concentration in accordance with current guidelines.

²The maximum concentrations analysed were limited by toxicity or to 5000 μg/plate (the maximum concentration in accordance with current guidelines) as appropriate in each strain, in the presence and absence of S9-mix.

³Test 2 to clarify result.

⁴Test 3 to confirm result.

NT—not tested.

n.b. SAR for all compounds is solely N-Nitro or N-Nitroso structure.

^*E. coli strain WP2uvrA (pkM101).

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