| . | . | . | . | . | ID-Now results . | BD Veritor results . | Quidel Sofia results . | Sekisui OSOM results . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dilution . | CFU/mL . | Volume of stock/dilution . | Volume of PBS . | Total Volume . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . |
| Stock | 3.7 × 10^7 | NA | NA | NA | Not tested | Pos | Pos | Pos | Not tested | Not tested | ||||||
| Additional dilution for BD | 1.5 × 10^7 | 415 ul of stock | 585 ul | 1000 uL | Not tested | Pos | Pos | Pos | Not tested | Not tested | ||||||
| Dil-1 | 1 × 10^7 | 555 ul of stock | 1445 ul | 2000 ul | Pos | Pos | Pos | Pos | Neg | Neg | Pos | Pos | Pos | Pos | Pos | Pos |
| Dil-2 | 1 × 10^6 | 200 ul of dil-1 | 1800 ul | 2000 ul | Pos | Pos | Pos | Neg | Neg | Neg | Pos | Neg | Pos | Neg | Neg | Neg |
| Dil-3 | 1 × 10^5 | 200 ul of dil-2 | 1800 ul | 2000 ul | Pos | Pos | Pos | Neg | Neg | Neg | ||||||
| Dil-4 | 5 × 10^4 | 900 ul of dil-3 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-5 | 2.5 × 10^4 | 900 ul of dil-4 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-6 | 1.25 × 10^4 | 900 ul of dil-5 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-7 | 6.25 × 10^3 | 900 ul of dil-6 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-8 | 3.125 × 10^3 | 900 ul of dil-7 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-9 | 1.562 × 10^3 | 900 ul of dil-8 | 900 ul | 1800 ul | Neg | Neg | Neg | |||||||||
| . | . | . | . | . | ID-Now results . | BD Veritor results . | Quidel Sofia results . | Sekisui OSOM results . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dilution . | CFU/mL . | Volume of stock/dilution . | Volume of PBS . | Total Volume . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . |
| Stock | 3.7 × 10^7 | NA | NA | NA | Not tested | Pos | Pos | Pos | Not tested | Not tested | ||||||
| Additional dilution for BD | 1.5 × 10^7 | 415 ul of stock | 585 ul | 1000 uL | Not tested | Pos | Pos | Pos | Not tested | Not tested | ||||||
| Dil-1 | 1 × 10^7 | 555 ul of stock | 1445 ul | 2000 ul | Pos | Pos | Pos | Pos | Neg | Neg | Pos | Pos | Pos | Pos | Pos | Pos |
| Dil-2 | 1 × 10^6 | 200 ul of dil-1 | 1800 ul | 2000 ul | Pos | Pos | Pos | Neg | Neg | Neg | Pos | Neg | Pos | Neg | Neg | Neg |
| Dil-3 | 1 × 10^5 | 200 ul of dil-2 | 1800 ul | 2000 ul | Pos | Pos | Pos | Neg | Neg | Neg | ||||||
| Dil-4 | 5 × 10^4 | 900 ul of dil-3 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-5 | 2.5 × 10^4 | 900 ul of dil-4 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-6 | 1.25 × 10^4 | 900 ul of dil-5 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-7 | 6.25 × 10^3 | 900 ul of dil-6 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-8 | 3.125 × 10^3 | 900 ul of dil-7 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-9 | 1.562 × 10^3 | 900 ul of dil-8 | 900 ul | 1800 ul | Neg | Neg | Neg | |||||||||
Bold indicates the lowest dilution where all 3 replicates were detected.
| . | . | . | . | . | ID-Now results . | BD Veritor results . | Quidel Sofia results . | Sekisui OSOM results . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dilution . | CFU/mL . | Volume of stock/dilution . | Volume of PBS . | Total Volume . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . |
| Stock | 3.7 × 10^7 | NA | NA | NA | Not tested | Pos | Pos | Pos | Not tested | Not tested | ||||||
| Additional dilution for BD | 1.5 × 10^7 | 415 ul of stock | 585 ul | 1000 uL | Not tested | Pos | Pos | Pos | Not tested | Not tested | ||||||
| Dil-1 | 1 × 10^7 | 555 ul of stock | 1445 ul | 2000 ul | Pos | Pos | Pos | Pos | Neg | Neg | Pos | Pos | Pos | Pos | Pos | Pos |
| Dil-2 | 1 × 10^6 | 200 ul of dil-1 | 1800 ul | 2000 ul | Pos | Pos | Pos | Neg | Neg | Neg | Pos | Neg | Pos | Neg | Neg | Neg |
| Dil-3 | 1 × 10^5 | 200 ul of dil-2 | 1800 ul | 2000 ul | Pos | Pos | Pos | Neg | Neg | Neg | ||||||
| Dil-4 | 5 × 10^4 | 900 ul of dil-3 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-5 | 2.5 × 10^4 | 900 ul of dil-4 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-6 | 1.25 × 10^4 | 900 ul of dil-5 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-7 | 6.25 × 10^3 | 900 ul of dil-6 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-8 | 3.125 × 10^3 | 900 ul of dil-7 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-9 | 1.562 × 10^3 | 900 ul of dil-8 | 900 ul | 1800 ul | Neg | Neg | Neg | |||||||||
| . | . | . | . | . | ID-Now results . | BD Veritor results . | Quidel Sofia results . | Sekisui OSOM results . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dilution . | CFU/mL . | Volume of stock/dilution . | Volume of PBS . | Total Volume . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . | Replicate-1 . | Replicate-2 . | Replicate-3 . |
| Stock | 3.7 × 10^7 | NA | NA | NA | Not tested | Pos | Pos | Pos | Not tested | Not tested | ||||||
| Additional dilution for BD | 1.5 × 10^7 | 415 ul of stock | 585 ul | 1000 uL | Not tested | Pos | Pos | Pos | Not tested | Not tested | ||||||
| Dil-1 | 1 × 10^7 | 555 ul of stock | 1445 ul | 2000 ul | Pos | Pos | Pos | Pos | Neg | Neg | Pos | Pos | Pos | Pos | Pos | Pos |
| Dil-2 | 1 × 10^6 | 200 ul of dil-1 | 1800 ul | 2000 ul | Pos | Pos | Pos | Neg | Neg | Neg | Pos | Neg | Pos | Neg | Neg | Neg |
| Dil-3 | 1 × 10^5 | 200 ul of dil-2 | 1800 ul | 2000 ul | Pos | Pos | Pos | Neg | Neg | Neg | ||||||
| Dil-4 | 5 × 10^4 | 900 ul of dil-3 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-5 | 2.5 × 10^4 | 900 ul of dil-4 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-6 | 1.25 × 10^4 | 900 ul of dil-5 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-7 | 6.25 × 10^3 | 900 ul of dil-6 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-8 | 3.125 × 10^3 | 900 ul of dil-7 | 900 ul | 1800 ul | Pos | Pos | Pos | |||||||||
| Dil-9 | 1.562 × 10^3 | 900 ul of dil-8 | 900 ul | 1800 ul | Neg | Neg | Neg | |||||||||
Bold indicates the lowest dilution where all 3 replicates were detected.
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