Table 1.

LoD determination of ATCC 12344.

     ID-Now resultsBD Veritor resultsQuidel Sofia resultsSekisui OSOM results
DilutionCFU/mLVolume of stock/dilutionVolume of PBSTotal VolumeReplicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3
Dil-11 × 10^7800 ul of stock1200 ul2000 ulPosPosPosPosPosPosPosPosPosPosPosPos
Dil-21 × 10^6200 ul of dil-11800 ul2000 ulPosPosPosNegNegNegPosPosPosNegNegNeg
Dil-31 × 10^5200 ul of dil-21800 ul2000 ulPosPosPosNegNegNeg
Dil-45 × 10^4900 ul of dil-3900 ul1800 ulPosPosPos
Dil-52.5 × 10^4900 ul of dil-4900 ul1800 ulPosPosPos
Dil-61.25 × 10^4900 ul of dil-5900 ul1800 ulPosPosPos
Dil-76.25 × 10^3900 ul of dil-6900 ul1800 ulPosPosPos
Dil-83.125 × 10^3900 ul of dil-7900 ul1800 ulNegPosPos
Dil-91.562 × 10^3900 ul of dil-8900 ul1800 ulNegPosNeg
     ID-Now resultsBD Veritor resultsQuidel Sofia resultsSekisui OSOM results
DilutionCFU/mLVolume of stock/dilutionVolume of PBSTotal VolumeReplicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3
Dil-11 × 10^7800 ul of stock1200 ul2000 ulPosPosPosPosPosPosPosPosPosPosPosPos
Dil-21 × 10^6200 ul of dil-11800 ul2000 ulPosPosPosNegNegNegPosPosPosNegNegNeg
Dil-31 × 10^5200 ul of dil-21800 ul2000 ulPosPosPosNegNegNeg
Dil-45 × 10^4900 ul of dil-3900 ul1800 ulPosPosPos
Dil-52.5 × 10^4900 ul of dil-4900 ul1800 ulPosPosPos
Dil-61.25 × 10^4900 ul of dil-5900 ul1800 ulPosPosPos
Dil-76.25 × 10^3900 ul of dil-6900 ul1800 ulPosPosPos
Dil-83.125 × 10^3900 ul of dil-7900 ul1800 ulNegPosPos
Dil-91.562 × 10^3900 ul of dil-8900 ul1800 ulNegPosNeg

Bold indicates the lowest dilution where all 3 replicates were detected.

Table 1.

LoD determination of ATCC 12344.

     ID-Now resultsBD Veritor resultsQuidel Sofia resultsSekisui OSOM results
DilutionCFU/mLVolume of stock/dilutionVolume of PBSTotal VolumeReplicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3
Dil-11 × 10^7800 ul of stock1200 ul2000 ulPosPosPosPosPosPosPosPosPosPosPosPos
Dil-21 × 10^6200 ul of dil-11800 ul2000 ulPosPosPosNegNegNegPosPosPosNegNegNeg
Dil-31 × 10^5200 ul of dil-21800 ul2000 ulPosPosPosNegNegNeg
Dil-45 × 10^4900 ul of dil-3900 ul1800 ulPosPosPos
Dil-52.5 × 10^4900 ul of dil-4900 ul1800 ulPosPosPos
Dil-61.25 × 10^4900 ul of dil-5900 ul1800 ulPosPosPos
Dil-76.25 × 10^3900 ul of dil-6900 ul1800 ulPosPosPos
Dil-83.125 × 10^3900 ul of dil-7900 ul1800 ulNegPosPos
Dil-91.562 × 10^3900 ul of dil-8900 ul1800 ulNegPosNeg
     ID-Now resultsBD Veritor resultsQuidel Sofia resultsSekisui OSOM results
DilutionCFU/mLVolume of stock/dilutionVolume of PBSTotal VolumeReplicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3Replicate-1Replicate-2Replicate-3
Dil-11 × 10^7800 ul of stock1200 ul2000 ulPosPosPosPosPosPosPosPosPosPosPosPos
Dil-21 × 10^6200 ul of dil-11800 ul2000 ulPosPosPosNegNegNegPosPosPosNegNegNeg
Dil-31 × 10^5200 ul of dil-21800 ul2000 ulPosPosPosNegNegNeg
Dil-45 × 10^4900 ul of dil-3900 ul1800 ulPosPosPos
Dil-52.5 × 10^4900 ul of dil-4900 ul1800 ulPosPosPos
Dil-61.25 × 10^4900 ul of dil-5900 ul1800 ulPosPosPos
Dil-76.25 × 10^3900 ul of dil-6900 ul1800 ulPosPosPos
Dil-83.125 × 10^3900 ul of dil-7900 ul1800 ulNegPosPos
Dil-91.562 × 10^3900 ul of dil-8900 ul1800 ulNegPosNeg

Bold indicates the lowest dilution where all 3 replicates were detected.

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