| Treatment . | Polyester . | Cotton . | ||||||
|---|---|---|---|---|---|---|---|---|
| Clean Condition . | Dirty Condition . | Clean Condition . | Dirty Condition . | |||||
| Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | |
| Virus titer | 4.5 ± 0.1 | … | 4.6 ± 0.2 | … | 4.0 ± 0.0 | … | 4.2 ± 0.1 | … |
| Water at 22°C | 3.3 ± 0.1 | 1.2 | 2.8 ± 0.2 | 1.9 | 2.6 ± 0.1 | 1.3 | 2.7 ± 0.0 | 1.5 |
| Water at 70°C | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Sodium hypochlorite 0.05% | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Liquid disinfectant | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Powder detergent 1 | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Powder detergent 2 | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Treatment . | Polyester . | Cotton . | ||||||
|---|---|---|---|---|---|---|---|---|
| Clean Condition . | Dirty Condition . | Clean Condition . | Dirty Condition . | |||||
| Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | |
| Virus titer | 4.5 ± 0.1 | … | 4.6 ± 0.2 | … | 4.0 ± 0.0 | … | 4.2 ± 0.1 | … |
| Water at 22°C | 3.3 ± 0.1 | 1.2 | 2.8 ± 0.2 | 1.9 | 2.6 ± 0.1 | 1.3 | 2.7 ± 0.0 | 1.5 |
| Water at 70°C | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Sodium hypochlorite 0.05% | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Liquid disinfectant | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Powder detergent 1 | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Powder detergent 2 | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
Data shows mean values (± SD) of 3 independent replicates. The limit of detection of the assay was 10 PFU/mL (1 log10 PFU/mL).
Abbreviations: LRV, log reduction value; PFU, plaque-forming unit.
The data presents the average log10 virus concentration and LRV of mpox virus in fabric (100% cotton and 100% polyester) with and without the washing intervention. Two conditions were assessed for each fabric: dirty (bovine serum albumin-containing media) and clean (phosphate-buffered saline-containing media). The evaluated conditions included 2 powder detergents (Ariel and OMO), a liquid sanitizer (Dettol), a 0.05% sodium hypochlorite solution, and water at room temperature and at 70°C.
| Treatment . | Polyester . | Cotton . | ||||||
|---|---|---|---|---|---|---|---|---|
| Clean Condition . | Dirty Condition . | Clean Condition . | Dirty Condition . | |||||
| Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | |
| Virus titer | 4.5 ± 0.1 | … | 4.6 ± 0.2 | … | 4.0 ± 0.0 | … | 4.2 ± 0.1 | … |
| Water at 22°C | 3.3 ± 0.1 | 1.2 | 2.8 ± 0.2 | 1.9 | 2.6 ± 0.1 | 1.3 | 2.7 ± 0.0 | 1.5 |
| Water at 70°C | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Sodium hypochlorite 0.05% | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Liquid disinfectant | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Powder detergent 1 | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Powder detergent 2 | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Treatment . | Polyester . | Cotton . | ||||||
|---|---|---|---|---|---|---|---|---|
| Clean Condition . | Dirty Condition . | Clean Condition . | Dirty Condition . | |||||
| Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | Concentration, log10 PFU . | LRV . | |
| Virus titer | 4.5 ± 0.1 | … | 4.6 ± 0.2 | … | 4.0 ± 0.0 | … | 4.2 ± 0.1 | … |
| Water at 22°C | 3.3 ± 0.1 | 1.2 | 2.8 ± 0.2 | 1.9 | 2.6 ± 0.1 | 1.3 | 2.7 ± 0.0 | 1.5 |
| Water at 70°C | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Sodium hypochlorite 0.05% | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Liquid disinfectant | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Powder detergent 1 | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
| Powder detergent 2 | ≤1.0 ± 0.0 | ≥3.5 | ≤1.0 ± 0.0 | ≥3.6 | ≤1.0 ± 0.0 | ≥3.0 | ≤1.0 ± 0.0 | ≥3.2 |
Data shows mean values (± SD) of 3 independent replicates. The limit of detection of the assay was 10 PFU/mL (1 log10 PFU/mL).
Abbreviations: LRV, log reduction value; PFU, plaque-forming unit.
The data presents the average log10 virus concentration and LRV of mpox virus in fabric (100% cotton and 100% polyester) with and without the washing intervention. Two conditions were assessed for each fabric: dirty (bovine serum albumin-containing media) and clean (phosphate-buffered saline-containing media). The evaluated conditions included 2 powder detergents (Ariel and OMO), a liquid sanitizer (Dettol), a 0.05% sodium hypochlorite solution, and water at room temperature and at 70°C.
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