Kinetic (Vmax, Km) and inhibition (KI) parameters for CH4 production from MeOH, H2, and acetate determined in concentrated whole-cell suspension assays of M. barkeri and methanogenic mixed cultures in response to increasing N2O concentrations.a
| Culture . | Substrate . | N2O (μM) . | Vmax (nmol CH4 min−1 mg protein−1) . | Km (μM) . | KI (μM) . |
|---|---|---|---|---|---|
| M. barkeri | MeOH | 0 | 440.4 ± 10.2 | 2.1 (±0.1) × 103 | 130.9 ± 4.7 |
| 100 | 244.1 ± 38.4 | ||||
| 200 | 188.8 ± 32.5 | ||||
| M. barkeri | H2 | 0 | 138.1 ± 6.9 | 51.1 ± 7.2 | 90.6 ± 10.8 |
| 50 | 82.8 ± 29.5 | ||||
| 100 | 59.9 ± 5.9 | ||||
| M. barkeri | Acetate | 0 | 40.8 ± 4.7 | 13.4 (±2.7) × 103 | 24.8 ± 3.1 |
| 20 | 32.1 ± 4.2 | ||||
| 50 | 22.8 ± 2.5 | ||||
| Mixed culture | MeOH | 0 | 209.0 ± 4.4 | 359.3 ± 30.8 | 109.9 ± 6.8 |
| 50 | 130.8 ± 22.6 | ||||
| 100 | 51.4 ± 44.7 | ||||
| Mixed culture | H2 | 0 | 105.8 ± 3.7 | 19.0 ± 2.3 | 62.1 ± 6.4 |
| 30 | 71.6 ± 10.4 | ||||
| 60 | 36.6 ± 13.6 | ||||
| Mixed culture | Acetate | 0 | 22.8 ± 0.7 | 302.2 ± 46.2 | 17.7 ± 1.8 |
| 10 | 14.1 ± 1.8 | ||||
| 30 | 5.7 ± 3.6 |
| Culture . | Substrate . | N2O (μM) . | Vmax (nmol CH4 min−1 mg protein−1) . | Km (μM) . | KI (μM) . |
|---|---|---|---|---|---|
| M. barkeri | MeOH | 0 | 440.4 ± 10.2 | 2.1 (±0.1) × 103 | 130.9 ± 4.7 |
| 100 | 244.1 ± 38.4 | ||||
| 200 | 188.8 ± 32.5 | ||||
| M. barkeri | H2 | 0 | 138.1 ± 6.9 | 51.1 ± 7.2 | 90.6 ± 10.8 |
| 50 | 82.8 ± 29.5 | ||||
| 100 | 59.9 ± 5.9 | ||||
| M. barkeri | Acetate | 0 | 40.8 ± 4.7 | 13.4 (±2.7) × 103 | 24.8 ± 3.1 |
| 20 | 32.1 ± 4.2 | ||||
| 50 | 22.8 ± 2.5 | ||||
| Mixed culture | MeOH | 0 | 209.0 ± 4.4 | 359.3 ± 30.8 | 109.9 ± 6.8 |
| 50 | 130.8 ± 22.6 | ||||
| 100 | 51.4 ± 44.7 | ||||
| Mixed culture | H2 | 0 | 105.8 ± 3.7 | 19.0 ± 2.3 | 62.1 ± 6.4 |
| 30 | 71.6 ± 10.4 | ||||
| 60 | 36.6 ± 13.6 | ||||
| Mixed culture | Acetate | 0 | 22.8 ± 0.7 | 302.2 ± 46.2 | 17.7 ± 1.8 |
| 10 | 14.1 ± 1.8 | ||||
| 30 | 5.7 ± 3.6 |
aData listed show results from the best fit Michaelis-Menten single-substrate-single-inhibitor models. Error values represent 95% confidence intervals.
Kinetic (Vmax, Km) and inhibition (KI) parameters for CH4 production from MeOH, H2, and acetate determined in concentrated whole-cell suspension assays of M. barkeri and methanogenic mixed cultures in response to increasing N2O concentrations.a
| Culture . | Substrate . | N2O (μM) . | Vmax (nmol CH4 min−1 mg protein−1) . | Km (μM) . | KI (μM) . |
|---|---|---|---|---|---|
| M. barkeri | MeOH | 0 | 440.4 ± 10.2 | 2.1 (±0.1) × 103 | 130.9 ± 4.7 |
| 100 | 244.1 ± 38.4 | ||||
| 200 | 188.8 ± 32.5 | ||||
| M. barkeri | H2 | 0 | 138.1 ± 6.9 | 51.1 ± 7.2 | 90.6 ± 10.8 |
| 50 | 82.8 ± 29.5 | ||||
| 100 | 59.9 ± 5.9 | ||||
| M. barkeri | Acetate | 0 | 40.8 ± 4.7 | 13.4 (±2.7) × 103 | 24.8 ± 3.1 |
| 20 | 32.1 ± 4.2 | ||||
| 50 | 22.8 ± 2.5 | ||||
| Mixed culture | MeOH | 0 | 209.0 ± 4.4 | 359.3 ± 30.8 | 109.9 ± 6.8 |
| 50 | 130.8 ± 22.6 | ||||
| 100 | 51.4 ± 44.7 | ||||
| Mixed culture | H2 | 0 | 105.8 ± 3.7 | 19.0 ± 2.3 | 62.1 ± 6.4 |
| 30 | 71.6 ± 10.4 | ||||
| 60 | 36.6 ± 13.6 | ||||
| Mixed culture | Acetate | 0 | 22.8 ± 0.7 | 302.2 ± 46.2 | 17.7 ± 1.8 |
| 10 | 14.1 ± 1.8 | ||||
| 30 | 5.7 ± 3.6 |
| Culture . | Substrate . | N2O (μM) . | Vmax (nmol CH4 min−1 mg protein−1) . | Km (μM) . | KI (μM) . |
|---|---|---|---|---|---|
| M. barkeri | MeOH | 0 | 440.4 ± 10.2 | 2.1 (±0.1) × 103 | 130.9 ± 4.7 |
| 100 | 244.1 ± 38.4 | ||||
| 200 | 188.8 ± 32.5 | ||||
| M. barkeri | H2 | 0 | 138.1 ± 6.9 | 51.1 ± 7.2 | 90.6 ± 10.8 |
| 50 | 82.8 ± 29.5 | ||||
| 100 | 59.9 ± 5.9 | ||||
| M. barkeri | Acetate | 0 | 40.8 ± 4.7 | 13.4 (±2.7) × 103 | 24.8 ± 3.1 |
| 20 | 32.1 ± 4.2 | ||||
| 50 | 22.8 ± 2.5 | ||||
| Mixed culture | MeOH | 0 | 209.0 ± 4.4 | 359.3 ± 30.8 | 109.9 ± 6.8 |
| 50 | 130.8 ± 22.6 | ||||
| 100 | 51.4 ± 44.7 | ||||
| Mixed culture | H2 | 0 | 105.8 ± 3.7 | 19.0 ± 2.3 | 62.1 ± 6.4 |
| 30 | 71.6 ± 10.4 | ||||
| 60 | 36.6 ± 13.6 | ||||
| Mixed culture | Acetate | 0 | 22.8 ± 0.7 | 302.2 ± 46.2 | 17.7 ± 1.8 |
| 10 | 14.1 ± 1.8 | ||||
| 30 | 5.7 ± 3.6 |
aData listed show results from the best fit Michaelis-Menten single-substrate-single-inhibitor models. Error values represent 95% confidence intervals.
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