Weights of Microbiology Culture Supplies and Greenhouse Gas Emissions Generated From Disposal
| . | Urine . | Blood . | ETT . | |||
|---|---|---|---|---|---|---|
| Supplies: Laboratory . | – . | + . | – . | + . | – . | + . |
| Gram stain slide,a g | ||||||
| Laboratory 1b | … | … | … | 9.4 | 9.4 | 9.4 |
| Laboratory 2c | … | … | … | 4.7 | 4.7 | 4.7 |
| Agar plates, g | ||||||
| Laboratory 1d | 56.8 | 85.2 | … | 113.6 | 113.6 | 142 |
| Laboratory 2 b,d | 31.2 | 55.4 | … | 88 | 88 | 117.3 |
| Blood culture bottles, g | ||||||
| Laboratory 1e | … | … | 125.4 | 125.4 | … | … |
| Laboratory 2f | … | … | 131.2 | 131.2 | … | … |
| Vent to enter bottle, g | ||||||
| Laboratory 1g | … | … | … | 3.98 | … | … |
| Laboratory 2f | … | … | … | 4.6 | … | … |
| Urine collection vessel, g | ||||||
| Laboratory 1d | 16.2 | 16.2 | … | … | … | … |
| Laboratory 2h | 17 | 17 | … | … | … | … |
| ETT collection vessel, g | ||||||
| Laboratory 1d | … | … | … | … | 35.4 | 35.4 |
| Laboratory 2d | … | … | … | … | 35.4 | 35.4 |
| Body fluids, 1 mL = 1 g | ||||||
| Laboratory 1 | 10 | 10 | 10 | 10 | 2 | 2 |
| Laboratory 2 | 10 | 10 | 10 | 10 | 2 | 2 |
| Disposable loop | ||||||
| Laboratory 1, No.; weight, gd | 0.92 | 0.92 | 0.92 | 0.92 | 0.92 | 0.92 |
| Laboratory 2, 10 or 100 μL, gc | 1.9 | 2.83 | … | 0.8 | 1.9 | 1.9 |
| Tube for injection | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2 | … | … | … | 10.9 | … | … |
| Tuberculin syringe to enter bottle | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2 | … | … | … | 3.9 | … | … |
| Pipette, g | ||||||
| Laboratory 1i | … | … | … | … | 2.5 | 2.5 |
| Laboratory 2c | … | … | … | … | 2 | 2 |
| Molecular identification kit, g | ||||||
| Laboratory 1j | … | … | … | 30 | … | … |
| Laboratory 2j | … | … | … | 30 | … | … |
| Syringe/tube | ||||||
| Laboratory 1: SafetyGlide syringe, gg | … | … | … | 4.78 | … | … |
| Laboratory 2: Tube for injection | … | … | … | 10.8 | … | … |
| Alcohol prep pad, g | ||||||
| Laboratory 1c | … | … | … | 0.72 | … | … |
| Laboratory 2c | … | … | … | 0.72 | … | … |
| Vitek 2 AST card and packaging | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2f | … | 21 | … | 21 | … | 21 |
| Tube/cap with fluid | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2c,f | … | 5.7 | … | 5.7 | … | 5.7 |
| MicroScan supplies,k g | ||||||
| Laboratory 1 | … | 90 | … | 90 | … | 90 |
| Laboratory 2 | … | … | … | … | … | … |
| Inoculator transfer,k g | ||||||
| Laboratory 1 | … | 26.4 | … | 26.4 | … | 26.4 |
| Laboratory 2 | … | … | … | … | … | … |
| Media, g | ||||||
| Laboratory 1 | … | 50.9 | … | 50.9 | … | 50.9 |
| Laboratory 2 | … | … | … | … | … | … |
| Total weight per culture, g | ||||||
| Laboratory 1 | 83.92 | 279.62 | 136.32 | 466.1 | 163.82 | 359.52 |
| Laboratory 2 | 60.1 | 111.93 | 141.2 | 322.32 | 134 | 190 |
| Greenhouse gas emissions, kg CO2 equivalent | ||||||
| Laboratory 1 | 0.05 | 0.16 | 0.08 | 0.26 | 0.09 | 0.2 |
| Laboratory 2 | 0.03 | 0.06 | 0.08 | 0.18 | 0.08 | 0.11 |
| Miles driven equivalent in gas-powered vehicle per culture | ||||||
| Laboratory 1 | 0.12 | 0.40 | 0.20 | 0.67 | 0.24 | 0.52 |
| Laboratory 2 | 0.09 | 0.16 | 0.20 | 0.46 | 0.19 | 0.27 |
| . | Urine . | Blood . | ETT . | |||
|---|---|---|---|---|---|---|
| Supplies: Laboratory . | – . | + . | – . | + . | – . | + . |
| Gram stain slide,a g | ||||||
| Laboratory 1b | … | … | … | 9.4 | 9.4 | 9.4 |
| Laboratory 2c | … | … | … | 4.7 | 4.7 | 4.7 |
| Agar plates, g | ||||||
| Laboratory 1d | 56.8 | 85.2 | … | 113.6 | 113.6 | 142 |
| Laboratory 2 b,d | 31.2 | 55.4 | … | 88 | 88 | 117.3 |
| Blood culture bottles, g | ||||||
| Laboratory 1e | … | … | 125.4 | 125.4 | … | … |
| Laboratory 2f | … | … | 131.2 | 131.2 | … | … |
| Vent to enter bottle, g | ||||||
| Laboratory 1g | … | … | … | 3.98 | … | … |
| Laboratory 2f | … | … | … | 4.6 | … | … |
| Urine collection vessel, g | ||||||
| Laboratory 1d | 16.2 | 16.2 | … | … | … | … |
| Laboratory 2h | 17 | 17 | … | … | … | … |
| ETT collection vessel, g | ||||||
| Laboratory 1d | … | … | … | … | 35.4 | 35.4 |
| Laboratory 2d | … | … | … | … | 35.4 | 35.4 |
| Body fluids, 1 mL = 1 g | ||||||
| Laboratory 1 | 10 | 10 | 10 | 10 | 2 | 2 |
| Laboratory 2 | 10 | 10 | 10 | 10 | 2 | 2 |
| Disposable loop | ||||||
| Laboratory 1, No.; weight, gd | 0.92 | 0.92 | 0.92 | 0.92 | 0.92 | 0.92 |
| Laboratory 2, 10 or 100 μL, gc | 1.9 | 2.83 | … | 0.8 | 1.9 | 1.9 |
| Tube for injection | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2 | … | … | … | 10.9 | … | … |
| Tuberculin syringe to enter bottle | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2 | … | … | … | 3.9 | … | … |
| Pipette, g | ||||||
| Laboratory 1i | … | … | … | … | 2.5 | 2.5 |
| Laboratory 2c | … | … | … | … | 2 | 2 |
| Molecular identification kit, g | ||||||
| Laboratory 1j | … | … | … | 30 | … | … |
| Laboratory 2j | … | … | … | 30 | … | … |
| Syringe/tube | ||||||
| Laboratory 1: SafetyGlide syringe, gg | … | … | … | 4.78 | … | … |
| Laboratory 2: Tube for injection | … | … | … | 10.8 | … | … |
| Alcohol prep pad, g | ||||||
| Laboratory 1c | … | … | … | 0.72 | … | … |
| Laboratory 2c | … | … | … | 0.72 | … | … |
| Vitek 2 AST card and packaging | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2f | … | 21 | … | 21 | … | 21 |
| Tube/cap with fluid | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2c,f | … | 5.7 | … | 5.7 | … | 5.7 |
| MicroScan supplies,k g | ||||||
| Laboratory 1 | … | 90 | … | 90 | … | 90 |
| Laboratory 2 | … | … | … | … | … | … |
| Inoculator transfer,k g | ||||||
| Laboratory 1 | … | 26.4 | … | 26.4 | … | 26.4 |
| Laboratory 2 | … | … | … | … | … | … |
| Media, g | ||||||
| Laboratory 1 | … | 50.9 | … | 50.9 | … | 50.9 |
| Laboratory 2 | … | … | … | … | … | … |
| Total weight per culture, g | ||||||
| Laboratory 1 | 83.92 | 279.62 | 136.32 | 466.1 | 163.82 | 359.52 |
| Laboratory 2 | 60.1 | 111.93 | 141.2 | 322.32 | 134 | 190 |
| Greenhouse gas emissions, kg CO2 equivalent | ||||||
| Laboratory 1 | 0.05 | 0.16 | 0.08 | 0.26 | 0.09 | 0.2 |
| Laboratory 2 | 0.03 | 0.06 | 0.08 | 0.18 | 0.08 | 0.11 |
| Miles driven equivalent in gas-powered vehicle per culture | ||||||
| Laboratory 1 | 0.12 | 0.40 | 0.20 | 0.67 | 0.24 | 0.52 |
| Laboratory 2 | 0.09 | 0.16 | 0.20 | 0.46 | 0.19 | 0.27 |
Laboratory 1, Children's Healthcare of Atlanta; laboratory 2, Nationwide Children's Hospital (NCH). For urine at NCH, a biplate agar was used (31.25 g/plate), but for blood and endotracheal tube cultures, a single agar was used (29.3 g/plate). Cells without values indicate that the material was not used or needed for this type of culture.
Abbreviation: ETT, endotracheal tube.
aLaboratory 1 uses slides in duplicate, and laboratory 2 uses single slides.
bRemel.
cFisher Scientific.
dCardinal Health and 28.4 g per agar plate.
eBACTEC (Plus Aerobic/F and Lytic/10 Anaerobic/F plastic bottles; Becton, Dickinson and Company).
fbioMérieux.
gBecton, Dickinson and Company.
hMedline.
iCopan Diagnostics.
jBioFire Diagnostics. Aluminum canister was recycled in both locations and thus not included in the final weight.
kIncludes additional disposable MicroScan supplies (Beckman Coulter).
Weights of Microbiology Culture Supplies and Greenhouse Gas Emissions Generated From Disposal
| . | Urine . | Blood . | ETT . | |||
|---|---|---|---|---|---|---|
| Supplies: Laboratory . | – . | + . | – . | + . | – . | + . |
| Gram stain slide,a g | ||||||
| Laboratory 1b | … | … | … | 9.4 | 9.4 | 9.4 |
| Laboratory 2c | … | … | … | 4.7 | 4.7 | 4.7 |
| Agar plates, g | ||||||
| Laboratory 1d | 56.8 | 85.2 | … | 113.6 | 113.6 | 142 |
| Laboratory 2 b,d | 31.2 | 55.4 | … | 88 | 88 | 117.3 |
| Blood culture bottles, g | ||||||
| Laboratory 1e | … | … | 125.4 | 125.4 | … | … |
| Laboratory 2f | … | … | 131.2 | 131.2 | … | … |
| Vent to enter bottle, g | ||||||
| Laboratory 1g | … | … | … | 3.98 | … | … |
| Laboratory 2f | … | … | … | 4.6 | … | … |
| Urine collection vessel, g | ||||||
| Laboratory 1d | 16.2 | 16.2 | … | … | … | … |
| Laboratory 2h | 17 | 17 | … | … | … | … |
| ETT collection vessel, g | ||||||
| Laboratory 1d | … | … | … | … | 35.4 | 35.4 |
| Laboratory 2d | … | … | … | … | 35.4 | 35.4 |
| Body fluids, 1 mL = 1 g | ||||||
| Laboratory 1 | 10 | 10 | 10 | 10 | 2 | 2 |
| Laboratory 2 | 10 | 10 | 10 | 10 | 2 | 2 |
| Disposable loop | ||||||
| Laboratory 1, No.; weight, gd | 0.92 | 0.92 | 0.92 | 0.92 | 0.92 | 0.92 |
| Laboratory 2, 10 or 100 μL, gc | 1.9 | 2.83 | … | 0.8 | 1.9 | 1.9 |
| Tube for injection | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2 | … | … | … | 10.9 | … | … |
| Tuberculin syringe to enter bottle | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2 | … | … | … | 3.9 | … | … |
| Pipette, g | ||||||
| Laboratory 1i | … | … | … | … | 2.5 | 2.5 |
| Laboratory 2c | … | … | … | … | 2 | 2 |
| Molecular identification kit, g | ||||||
| Laboratory 1j | … | … | … | 30 | … | … |
| Laboratory 2j | … | … | … | 30 | … | … |
| Syringe/tube | ||||||
| Laboratory 1: SafetyGlide syringe, gg | … | … | … | 4.78 | … | … |
| Laboratory 2: Tube for injection | … | … | … | 10.8 | … | … |
| Alcohol prep pad, g | ||||||
| Laboratory 1c | … | … | … | 0.72 | … | … |
| Laboratory 2c | … | … | … | 0.72 | … | … |
| Vitek 2 AST card and packaging | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2f | … | 21 | … | 21 | … | 21 |
| Tube/cap with fluid | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2c,f | … | 5.7 | … | 5.7 | … | 5.7 |
| MicroScan supplies,k g | ||||||
| Laboratory 1 | … | 90 | … | 90 | … | 90 |
| Laboratory 2 | … | … | … | … | … | … |
| Inoculator transfer,k g | ||||||
| Laboratory 1 | … | 26.4 | … | 26.4 | … | 26.4 |
| Laboratory 2 | … | … | … | … | … | … |
| Media, g | ||||||
| Laboratory 1 | … | 50.9 | … | 50.9 | … | 50.9 |
| Laboratory 2 | … | … | … | … | … | … |
| Total weight per culture, g | ||||||
| Laboratory 1 | 83.92 | 279.62 | 136.32 | 466.1 | 163.82 | 359.52 |
| Laboratory 2 | 60.1 | 111.93 | 141.2 | 322.32 | 134 | 190 |
| Greenhouse gas emissions, kg CO2 equivalent | ||||||
| Laboratory 1 | 0.05 | 0.16 | 0.08 | 0.26 | 0.09 | 0.2 |
| Laboratory 2 | 0.03 | 0.06 | 0.08 | 0.18 | 0.08 | 0.11 |
| Miles driven equivalent in gas-powered vehicle per culture | ||||||
| Laboratory 1 | 0.12 | 0.40 | 0.20 | 0.67 | 0.24 | 0.52 |
| Laboratory 2 | 0.09 | 0.16 | 0.20 | 0.46 | 0.19 | 0.27 |
| . | Urine . | Blood . | ETT . | |||
|---|---|---|---|---|---|---|
| Supplies: Laboratory . | – . | + . | – . | + . | – . | + . |
| Gram stain slide,a g | ||||||
| Laboratory 1b | … | … | … | 9.4 | 9.4 | 9.4 |
| Laboratory 2c | … | … | … | 4.7 | 4.7 | 4.7 |
| Agar plates, g | ||||||
| Laboratory 1d | 56.8 | 85.2 | … | 113.6 | 113.6 | 142 |
| Laboratory 2 b,d | 31.2 | 55.4 | … | 88 | 88 | 117.3 |
| Blood culture bottles, g | ||||||
| Laboratory 1e | … | … | 125.4 | 125.4 | … | … |
| Laboratory 2f | … | … | 131.2 | 131.2 | … | … |
| Vent to enter bottle, g | ||||||
| Laboratory 1g | … | … | … | 3.98 | … | … |
| Laboratory 2f | … | … | … | 4.6 | … | … |
| Urine collection vessel, g | ||||||
| Laboratory 1d | 16.2 | 16.2 | … | … | … | … |
| Laboratory 2h | 17 | 17 | … | … | … | … |
| ETT collection vessel, g | ||||||
| Laboratory 1d | … | … | … | … | 35.4 | 35.4 |
| Laboratory 2d | … | … | … | … | 35.4 | 35.4 |
| Body fluids, 1 mL = 1 g | ||||||
| Laboratory 1 | 10 | 10 | 10 | 10 | 2 | 2 |
| Laboratory 2 | 10 | 10 | 10 | 10 | 2 | 2 |
| Disposable loop | ||||||
| Laboratory 1, No.; weight, gd | 0.92 | 0.92 | 0.92 | 0.92 | 0.92 | 0.92 |
| Laboratory 2, 10 or 100 μL, gc | 1.9 | 2.83 | … | 0.8 | 1.9 | 1.9 |
| Tube for injection | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2 | … | … | … | 10.9 | … | … |
| Tuberculin syringe to enter bottle | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2 | … | … | … | 3.9 | … | … |
| Pipette, g | ||||||
| Laboratory 1i | … | … | … | … | 2.5 | 2.5 |
| Laboratory 2c | … | … | … | … | 2 | 2 |
| Molecular identification kit, g | ||||||
| Laboratory 1j | … | … | … | 30 | … | … |
| Laboratory 2j | … | … | … | 30 | … | … |
| Syringe/tube | ||||||
| Laboratory 1: SafetyGlide syringe, gg | … | … | … | 4.78 | … | … |
| Laboratory 2: Tube for injection | … | … | … | 10.8 | … | … |
| Alcohol prep pad, g | ||||||
| Laboratory 1c | … | … | … | 0.72 | … | … |
| Laboratory 2c | … | … | … | 0.72 | … | … |
| Vitek 2 AST card and packaging | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2f | … | 21 | … | 21 | … | 21 |
| Tube/cap with fluid | ||||||
| Laboratory 1 | … | … | … | … | … | … |
| Laboratory 2c,f | … | 5.7 | … | 5.7 | … | 5.7 |
| MicroScan supplies,k g | ||||||
| Laboratory 1 | … | 90 | … | 90 | … | 90 |
| Laboratory 2 | … | … | … | … | … | … |
| Inoculator transfer,k g | ||||||
| Laboratory 1 | … | 26.4 | … | 26.4 | … | 26.4 |
| Laboratory 2 | … | … | … | … | … | … |
| Media, g | ||||||
| Laboratory 1 | … | 50.9 | … | 50.9 | … | 50.9 |
| Laboratory 2 | … | … | … | … | … | … |
| Total weight per culture, g | ||||||
| Laboratory 1 | 83.92 | 279.62 | 136.32 | 466.1 | 163.82 | 359.52 |
| Laboratory 2 | 60.1 | 111.93 | 141.2 | 322.32 | 134 | 190 |
| Greenhouse gas emissions, kg CO2 equivalent | ||||||
| Laboratory 1 | 0.05 | 0.16 | 0.08 | 0.26 | 0.09 | 0.2 |
| Laboratory 2 | 0.03 | 0.06 | 0.08 | 0.18 | 0.08 | 0.11 |
| Miles driven equivalent in gas-powered vehicle per culture | ||||||
| Laboratory 1 | 0.12 | 0.40 | 0.20 | 0.67 | 0.24 | 0.52 |
| Laboratory 2 | 0.09 | 0.16 | 0.20 | 0.46 | 0.19 | 0.27 |
Laboratory 1, Children's Healthcare of Atlanta; laboratory 2, Nationwide Children's Hospital (NCH). For urine at NCH, a biplate agar was used (31.25 g/plate), but for blood and endotracheal tube cultures, a single agar was used (29.3 g/plate). Cells without values indicate that the material was not used or needed for this type of culture.
Abbreviation: ETT, endotracheal tube.
aLaboratory 1 uses slides in duplicate, and laboratory 2 uses single slides.
bRemel.
cFisher Scientific.
dCardinal Health and 28.4 g per agar plate.
eBACTEC (Plus Aerobic/F and Lytic/10 Anaerobic/F plastic bottles; Becton, Dickinson and Company).
fbioMérieux.
gBecton, Dickinson and Company.
hMedline.
iCopan Diagnostics.
jBioFire Diagnostics. Aluminum canister was recycled in both locations and thus not included in the final weight.
kIncludes additional disposable MicroScan supplies (Beckman Coulter).
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