Table 2.

Products of the plasmid-encoded metal-resistance determinantsa

Locus tagGeneCH34_0CH34_MCH34_EDescription
Plasmid pMOL28:
pMOL28 mer: not found merR, merT, merD, and merE 
Rmet_6346merPNF3 935 ± 175748Periplasmic mercury-binding protein
Rmet_6183merANF11 249 ± 2 250NFA6UXG5 Mercuric reductase
chr: not found chrZ, chrP, chrF1, chrA1, and chrI 
Rmet_6195chrYNF263 ± 152NFQ1L9 X 2 Putative uncharacterized protein
Rmet_6197chrNNF129 ± 77NFQ1L9 X 0 Putative uncharacterized protein
Rmet_6198chrONF727 ± 23917Q1L9W9 Putative uncharacterized protein
Rmet_6200chrENF314 ± 199NFQ5NUZ8 Superoxide dismutase SodM
Rmet_6201chrC1041 318 ± 1 154 (12.7; 1.1)NFP17550 Superoxide dismutase (Fe)
Rmet_6203chrB1NF649 ± 516NFP17552 Protein ChrB
cnr: not found cnrY, cnrC, and cnrA 
Rmet_6206cnrX44388 ± 176NFP37975 Nickel sensor of the antisigmafactor complex
Rmet_6207cnrH67NFNFP37978 RNA polymerase sigma factor CnrH
Rmet_6209cnrB2071 235 ± 272NFP37973 Nickel and cobalt-resistance protein CnrB
Rmet_6211cnrT11NFNFQ9L3G0 CnrT protein
Plasmid pMOL30: neither ncc nor sil products found
pbr: not found pbrU, pbrR, pbrA, pbrB/C, and pbrD 
Rmet_5945pbrT206 ± 156223 ± 47 (1.1; 0.1)847 ± 353 (4.1; 1.3)Q58AJ4 PbrT protein (iron permease FTR1)
czc region: not found flgB, ompP, czcJ, czcD, czcI, czcN, and czcM
Rmet_5970czcP31 ± 1976NFQ1LAJ7 Heavy metal translocating P-type ATPase
Rmet_5976czcENF96NFQ1LAJ1 Putative uncharacterized protein
Rmet_5977czcSNF313 ± 270NFQ44007 Sensor protein CzcS
Rmet_5978czcR67566 ± 539 (8.5; 0.9)89 ± 52 (1.3; 0.4)Q44006 Transcriptional activator protein CzcR
Rmet_5980czcA95854 ± 489 (9.0; 1.4)75 ± 50 (0.8; 0.2)P13511 Cobalt–zinc–cadmium-resistance protein CzcA
Rmet_5981czcB146 ± 1041 509 ± 607 (10.4; 1.9)113 ± 26 (0.8; 0.2)P13510 Cobalt–zinc–cadmium-resistance protein CzcB
Rmet_5982czcC17391 ± 207 (22.7; 1.8)NFP13509 Cobalt–zinc–cadmium-resistance protein CzcC
cop1: not found copV, copT, copM, copK, copD1, copJ, copG, copL, copQ, copE, and copW
Rmet_6109copNNF112 ± 25NFQ1LA58 Putative uncharacterized protein
Rmet_6110copS1NF467 ± 471NFQ58AD4 Sensor protein
Rmet_6111copR121 ± 9322 ± 12522 ± 8 (1.0; 0.1)Q58AD5 Two-component regulator
Rmet_6112copA1NF1 054 ± 623NFQ58AD6 Copper-resistance protein CopA
Rmet_6113copB1NF1 559 ± 870NFQ58AD7 CopB protein (copper-resistance B)
Rmet_6114copC1NF704 ± 120NFQ1LA53 Copper-resistance protein CopC
Rmet_6116copINF2 274 ± 982NFQ58AE0 Putative oxydoreductase
Rmet_6119copF195 ± 116768 ± 701 (3.9; 0.7)46 ± 21 (0.2; 1.1)Q58AE3 Heavy metal translocating P-type ATPase
Rmet_6122copHNF5 301 ± 2 83736Q58AE5 CopH protein
pMOL30 mer1: no products found 
pMOL30 mer2: not found merT, merD, and merE 
Rmet_6171merR117 ± 68151 ± 102 (1.3; 0.2)146 ± 38 (1.3; 0.3)P69413 Mercuric-resistance operon regulatory protein
Rmet_6173merPNF3 935 ± 1 75748Q58AI1 Periplasmic mercuric ion-binding protein
Rmet_6174merANF249 ± 147NFQ1L9Z3 Mercuric reductase MerA
Locus tagGeneCH34_0CH34_MCH34_EDescription
Plasmid pMOL28:
pMOL28 mer: not found merR, merT, merD, and merE 
Rmet_6346merPNF3 935 ± 175748Periplasmic mercury-binding protein
Rmet_6183merANF11 249 ± 2 250NFA6UXG5 Mercuric reductase
chr: not found chrZ, chrP, chrF1, chrA1, and chrI 
Rmet_6195chrYNF263 ± 152NFQ1L9 X 2 Putative uncharacterized protein
Rmet_6197chrNNF129 ± 77NFQ1L9 X 0 Putative uncharacterized protein
Rmet_6198chrONF727 ± 23917Q1L9W9 Putative uncharacterized protein
Rmet_6200chrENF314 ± 199NFQ5NUZ8 Superoxide dismutase SodM
Rmet_6201chrC1041 318 ± 1 154 (12.7; 1.1)NFP17550 Superoxide dismutase (Fe)
Rmet_6203chrB1NF649 ± 516NFP17552 Protein ChrB
cnr: not found cnrY, cnrC, and cnrA 
Rmet_6206cnrX44388 ± 176NFP37975 Nickel sensor of the antisigmafactor complex
Rmet_6207cnrH67NFNFP37978 RNA polymerase sigma factor CnrH
Rmet_6209cnrB2071 235 ± 272NFP37973 Nickel and cobalt-resistance protein CnrB
Rmet_6211cnrT11NFNFQ9L3G0 CnrT protein
Plasmid pMOL30: neither ncc nor sil products found
pbr: not found pbrU, pbrR, pbrA, pbrB/C, and pbrD 
Rmet_5945pbrT206 ± 156223 ± 47 (1.1; 0.1)847 ± 353 (4.1; 1.3)Q58AJ4 PbrT protein (iron permease FTR1)
czc region: not found flgB, ompP, czcJ, czcD, czcI, czcN, and czcM
Rmet_5970czcP31 ± 1976NFQ1LAJ7 Heavy metal translocating P-type ATPase
Rmet_5976czcENF96NFQ1LAJ1 Putative uncharacterized protein
Rmet_5977czcSNF313 ± 270NFQ44007 Sensor protein CzcS
Rmet_5978czcR67566 ± 539 (8.5; 0.9)89 ± 52 (1.3; 0.4)Q44006 Transcriptional activator protein CzcR
Rmet_5980czcA95854 ± 489 (9.0; 1.4)75 ± 50 (0.8; 0.2)P13511 Cobalt–zinc–cadmium-resistance protein CzcA
Rmet_5981czcB146 ± 1041 509 ± 607 (10.4; 1.9)113 ± 26 (0.8; 0.2)P13510 Cobalt–zinc–cadmium-resistance protein CzcB
Rmet_5982czcC17391 ± 207 (22.7; 1.8)NFP13509 Cobalt–zinc–cadmium-resistance protein CzcC
cop1: not found copV, copT, copM, copK, copD1, copJ, copG, copL, copQ, copE, and copW
Rmet_6109copNNF112 ± 25NFQ1LA58 Putative uncharacterized protein
Rmet_6110copS1NF467 ± 471NFQ58AD4 Sensor protein
Rmet_6111copR121 ± 9322 ± 12522 ± 8 (1.0; 0.1)Q58AD5 Two-component regulator
Rmet_6112copA1NF1 054 ± 623NFQ58AD6 Copper-resistance protein CopA
Rmet_6113copB1NF1 559 ± 870NFQ58AD7 CopB protein (copper-resistance B)
Rmet_6114copC1NF704 ± 120NFQ1LA53 Copper-resistance protein CopC
Rmet_6116copINF2 274 ± 982NFQ58AE0 Putative oxydoreductase
Rmet_6119copF195 ± 116768 ± 701 (3.9; 0.7)46 ± 21 (0.2; 1.1)Q58AE3 Heavy metal translocating P-type ATPase
Rmet_6122copHNF5 301 ± 2 83736Q58AE5 CopH protein
pMOL30 mer1: no products found 
pMOL30 mer2: not found merT, merD, and merE 
Rmet_6171merR117 ± 68151 ± 102 (1.3; 0.2)146 ± 38 (1.3; 0.3)P69413 Mercuric-resistance operon regulatory protein
Rmet_6173merPNF3 935 ± 1 75748Q58AI1 Periplasmic mercuric ion-binding protein
Rmet_6174merANF249 ± 147NFQ1L9Z3 Mercuric reductase MerA

aThe copy numbers per cell of the products of plasmid-encoded metal-resistance determinants are given for C. metallidurans strain CH34 cultivated without added substance (CH34_0), metal-shocked (CH34_M) and metal-starvation conditions (CH34_E) with the mean values and deviations. Numbers without deviations indicate proteins determined just once in the respective triplicate determination. The copy numbers are followed by the ratios Q and the distance value D for the comparison of CH34_M and of CH34_E with CH34_0. These values were not provided when the respective protein could not be measured in CH34_0. NF, not found.

Table 2.

Products of the plasmid-encoded metal-resistance determinantsa

Locus tagGeneCH34_0CH34_MCH34_EDescription
Plasmid pMOL28:
pMOL28 mer: not found merR, merT, merD, and merE 
Rmet_6346merPNF3 935 ± 175748Periplasmic mercury-binding protein
Rmet_6183merANF11 249 ± 2 250NFA6UXG5 Mercuric reductase
chr: not found chrZ, chrP, chrF1, chrA1, and chrI 
Rmet_6195chrYNF263 ± 152NFQ1L9 X 2 Putative uncharacterized protein
Rmet_6197chrNNF129 ± 77NFQ1L9 X 0 Putative uncharacterized protein
Rmet_6198chrONF727 ± 23917Q1L9W9 Putative uncharacterized protein
Rmet_6200chrENF314 ± 199NFQ5NUZ8 Superoxide dismutase SodM
Rmet_6201chrC1041 318 ± 1 154 (12.7; 1.1)NFP17550 Superoxide dismutase (Fe)
Rmet_6203chrB1NF649 ± 516NFP17552 Protein ChrB
cnr: not found cnrY, cnrC, and cnrA 
Rmet_6206cnrX44388 ± 176NFP37975 Nickel sensor of the antisigmafactor complex
Rmet_6207cnrH67NFNFP37978 RNA polymerase sigma factor CnrH
Rmet_6209cnrB2071 235 ± 272NFP37973 Nickel and cobalt-resistance protein CnrB
Rmet_6211cnrT11NFNFQ9L3G0 CnrT protein
Plasmid pMOL30: neither ncc nor sil products found
pbr: not found pbrU, pbrR, pbrA, pbrB/C, and pbrD 
Rmet_5945pbrT206 ± 156223 ± 47 (1.1; 0.1)847 ± 353 (4.1; 1.3)Q58AJ4 PbrT protein (iron permease FTR1)
czc region: not found flgB, ompP, czcJ, czcD, czcI, czcN, and czcM
Rmet_5970czcP31 ± 1976NFQ1LAJ7 Heavy metal translocating P-type ATPase
Rmet_5976czcENF96NFQ1LAJ1 Putative uncharacterized protein
Rmet_5977czcSNF313 ± 270NFQ44007 Sensor protein CzcS
Rmet_5978czcR67566 ± 539 (8.5; 0.9)89 ± 52 (1.3; 0.4)Q44006 Transcriptional activator protein CzcR
Rmet_5980czcA95854 ± 489 (9.0; 1.4)75 ± 50 (0.8; 0.2)P13511 Cobalt–zinc–cadmium-resistance protein CzcA
Rmet_5981czcB146 ± 1041 509 ± 607 (10.4; 1.9)113 ± 26 (0.8; 0.2)P13510 Cobalt–zinc–cadmium-resistance protein CzcB
Rmet_5982czcC17391 ± 207 (22.7; 1.8)NFP13509 Cobalt–zinc–cadmium-resistance protein CzcC
cop1: not found copV, copT, copM, copK, copD1, copJ, copG, copL, copQ, copE, and copW
Rmet_6109copNNF112 ± 25NFQ1LA58 Putative uncharacterized protein
Rmet_6110copS1NF467 ± 471NFQ58AD4 Sensor protein
Rmet_6111copR121 ± 9322 ± 12522 ± 8 (1.0; 0.1)Q58AD5 Two-component regulator
Rmet_6112copA1NF1 054 ± 623NFQ58AD6 Copper-resistance protein CopA
Rmet_6113copB1NF1 559 ± 870NFQ58AD7 CopB protein (copper-resistance B)
Rmet_6114copC1NF704 ± 120NFQ1LA53 Copper-resistance protein CopC
Rmet_6116copINF2 274 ± 982NFQ58AE0 Putative oxydoreductase
Rmet_6119copF195 ± 116768 ± 701 (3.9; 0.7)46 ± 21 (0.2; 1.1)Q58AE3 Heavy metal translocating P-type ATPase
Rmet_6122copHNF5 301 ± 2 83736Q58AE5 CopH protein
pMOL30 mer1: no products found 
pMOL30 mer2: not found merT, merD, and merE 
Rmet_6171merR117 ± 68151 ± 102 (1.3; 0.2)146 ± 38 (1.3; 0.3)P69413 Mercuric-resistance operon regulatory protein
Rmet_6173merPNF3 935 ± 1 75748Q58AI1 Periplasmic mercuric ion-binding protein
Rmet_6174merANF249 ± 147NFQ1L9Z3 Mercuric reductase MerA
Locus tagGeneCH34_0CH34_MCH34_EDescription
Plasmid pMOL28:
pMOL28 mer: not found merR, merT, merD, and merE 
Rmet_6346merPNF3 935 ± 175748Periplasmic mercury-binding protein
Rmet_6183merANF11 249 ± 2 250NFA6UXG5 Mercuric reductase
chr: not found chrZ, chrP, chrF1, chrA1, and chrI 
Rmet_6195chrYNF263 ± 152NFQ1L9 X 2 Putative uncharacterized protein
Rmet_6197chrNNF129 ± 77NFQ1L9 X 0 Putative uncharacterized protein
Rmet_6198chrONF727 ± 23917Q1L9W9 Putative uncharacterized protein
Rmet_6200chrENF314 ± 199NFQ5NUZ8 Superoxide dismutase SodM
Rmet_6201chrC1041 318 ± 1 154 (12.7; 1.1)NFP17550 Superoxide dismutase (Fe)
Rmet_6203chrB1NF649 ± 516NFP17552 Protein ChrB
cnr: not found cnrY, cnrC, and cnrA 
Rmet_6206cnrX44388 ± 176NFP37975 Nickel sensor of the antisigmafactor complex
Rmet_6207cnrH67NFNFP37978 RNA polymerase sigma factor CnrH
Rmet_6209cnrB2071 235 ± 272NFP37973 Nickel and cobalt-resistance protein CnrB
Rmet_6211cnrT11NFNFQ9L3G0 CnrT protein
Plasmid pMOL30: neither ncc nor sil products found
pbr: not found pbrU, pbrR, pbrA, pbrB/C, and pbrD 
Rmet_5945pbrT206 ± 156223 ± 47 (1.1; 0.1)847 ± 353 (4.1; 1.3)Q58AJ4 PbrT protein (iron permease FTR1)
czc region: not found flgB, ompP, czcJ, czcD, czcI, czcN, and czcM
Rmet_5970czcP31 ± 1976NFQ1LAJ7 Heavy metal translocating P-type ATPase
Rmet_5976czcENF96NFQ1LAJ1 Putative uncharacterized protein
Rmet_5977czcSNF313 ± 270NFQ44007 Sensor protein CzcS
Rmet_5978czcR67566 ± 539 (8.5; 0.9)89 ± 52 (1.3; 0.4)Q44006 Transcriptional activator protein CzcR
Rmet_5980czcA95854 ± 489 (9.0; 1.4)75 ± 50 (0.8; 0.2)P13511 Cobalt–zinc–cadmium-resistance protein CzcA
Rmet_5981czcB146 ± 1041 509 ± 607 (10.4; 1.9)113 ± 26 (0.8; 0.2)P13510 Cobalt–zinc–cadmium-resistance protein CzcB
Rmet_5982czcC17391 ± 207 (22.7; 1.8)NFP13509 Cobalt–zinc–cadmium-resistance protein CzcC
cop1: not found copV, copT, copM, copK, copD1, copJ, copG, copL, copQ, copE, and copW
Rmet_6109copNNF112 ± 25NFQ1LA58 Putative uncharacterized protein
Rmet_6110copS1NF467 ± 471NFQ58AD4 Sensor protein
Rmet_6111copR121 ± 9322 ± 12522 ± 8 (1.0; 0.1)Q58AD5 Two-component regulator
Rmet_6112copA1NF1 054 ± 623NFQ58AD6 Copper-resistance protein CopA
Rmet_6113copB1NF1 559 ± 870NFQ58AD7 CopB protein (copper-resistance B)
Rmet_6114copC1NF704 ± 120NFQ1LA53 Copper-resistance protein CopC
Rmet_6116copINF2 274 ± 982NFQ58AE0 Putative oxydoreductase
Rmet_6119copF195 ± 116768 ± 701 (3.9; 0.7)46 ± 21 (0.2; 1.1)Q58AE3 Heavy metal translocating P-type ATPase
Rmet_6122copHNF5 301 ± 2 83736Q58AE5 CopH protein
pMOL30 mer1: no products found 
pMOL30 mer2: not found merT, merD, and merE 
Rmet_6171merR117 ± 68151 ± 102 (1.3; 0.2)146 ± 38 (1.3; 0.3)P69413 Mercuric-resistance operon regulatory protein
Rmet_6173merPNF3 935 ± 1 75748Q58AI1 Periplasmic mercuric ion-binding protein
Rmet_6174merANF249 ± 147NFQ1L9Z3 Mercuric reductase MerA

aThe copy numbers per cell of the products of plasmid-encoded metal-resistance determinants are given for C. metallidurans strain CH34 cultivated without added substance (CH34_0), metal-shocked (CH34_M) and metal-starvation conditions (CH34_E) with the mean values and deviations. Numbers without deviations indicate proteins determined just once in the respective triplicate determination. The copy numbers are followed by the ratios Q and the distance value D for the comparison of CH34_M and of CH34_E with CH34_0. These values were not provided when the respective protein could not be measured in CH34_0. NF, not found.

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