Table 2. Open in new tab Metal...

		Metals present in the strain-specific MultiTox metal mix
CH34	No addition	4.8 ± 3.5	27 ± 10	20 ± 12	19 ± 10	83 ± 10	bQ	bQ	bQ
CH34	EDTA	bQ	8 ± 4	5 ± 3	7 ± 3	50 ± 12	bQ	bQ	bQ
CH34	MetalMix	75.6 ± 29.2	727 ± 278	1343 ± 338	1587 ± 543	3558 ± 2283	3746 ± 1 380	85 ± 53	27 ± 6
Chal. Conc., (µM)^b	19	347	761	241	461	1503	15	0.37
AE104	no addition	bQ	27 ± 2	7 ± 3	10 ± 2	70 ± 16	bQ	bQ	bQ
AE104	EDTA	bQ	18 ± 2	7 ± 3	11 ± 5	59 ± 12	bQ	bQ	bQ
AE104	MetalMix	37.9 ± 8.7	61 ± 16	59 ± 18	2035 ± 2251	92 ± 9	2034 ± 1 933	236 ± 233	21 ± 11
Chal. Conc., (µM)^b	7	7	9	120	5	849	3	0.18
		Metals not present in the MultiTox metal mix, but present in the mineral salts medium used (TMM)
Strain	condition metal	10⁶ Mg	10³ Ca	Mn	10³ Fe	Mo
CH34	No addition	10.7 ± 1.2	212 ± 92	531 ± 157	735 ± 80	2488 ± 359
CH34	EDTA	9.6 ± 0.3	117 ± 23	680 ± 295	636 ± 60	2117 ± 100
CH34	MetalMix	9.0 ± 0.5	213 ± 75	833 ± 311	609 ± 61	922 ± 41
AE104	No addition	10.0 ± 0.9	164 ± 17	464 ± 175	740 ± 36	2670 ± 272
AE104	EDTA	9.4 ± 0.4	154 ± 21	328 ± 134	675 ± 48	2378 ± 100
AE104	MetalMix	10.0 ± 0.5	153 ± 41	491 ± 340	652 ± 55	2222 ± 48

Metals present in the strain-specific MultiTox metal mix

Strain

condition Metal/cell

10³ Cr

10³ Co

10³ Ni

10³ Cu

10³ Zn

10³ As

10³ Cd

10³ Hg

CH34

No addition

4.8 ± 3.5

27 ± 10

20 ± 12

19 ± 10

83 ± 10

CH34

EDTA

8 ± 4

5 ± 3

7 ± 3

50 ± 12

CH34

MetalMix

75.6 ± 29.2

727 ± 278

1343 ± 338

1587 ± 543

3558 ± 2283

3746 ± 1 380

85 ± 53

27 ± 6

Chal. Conc., (µM)^b

347

761

241

461

1503

0.37

AE104

no addition

27 ± 2

7 ± 3

10 ± 2

70 ± 16

AE104

EDTA

18 ± 2

7 ± 3

11 ± 5

59 ± 12

AE104

MetalMix

37.9 ± 8.7

61 ± 16

59 ± 18

2035 ± 2251

92 ± 9

2034 ± 1 933

236 ± 233

21 ± 11

Chal. Conc., (µM)^b

120

849

0.18

Metals not present in the MultiTox metal mix, but present in the mineral salts medium used (TMM)

Strain

condition metal

10⁶ Mg

10³ Ca

10³ Fe

CH34

No addition

10.7 ± 1.2

212 ± 92

531 ± 157

735 ± 80

2488 ± 359

CH34

EDTA

9.6 ± 0.3

117 ± 23

680 ± 295

636 ± 60

2117 ± 100

CH34

MetalMix

9.0 ± 0.5

213 ± 75

833 ± 311

609 ± 61

922 ± 41

AE104

No addition

10.0 ± 0.9

164 ± 17

464 ± 175

740 ± 36

2670 ± 272

AE104

EDTA

9.4 ± 0.4

154 ± 21

328 ± 134

675 ± 48

2378 ± 100

AE104

MetalMix

10.0 ± 0.5

153 ± 41

491 ± 340

652 ± 55

2222 ± 48

		Metals present in the strain-specific MultiTox metal mix
CH34	No addition	4.8 ± 3.5	27 ± 10	20 ± 12	19 ± 10	83 ± 10	bQ	bQ	bQ
CH34	EDTA	bQ	8 ± 4	5 ± 3	7 ± 3	50 ± 12	bQ	bQ	bQ
CH34	MetalMix	75.6 ± 29.2	727 ± 278	1343 ± 338	1587 ± 543	3558 ± 2283	3746 ± 1 380	85 ± 53	27 ± 6
Chal. Conc., (µM)^b	19	347	761	241	461	1503	15	0.37
AE104	no addition	bQ	27 ± 2	7 ± 3	10 ± 2	70 ± 16	bQ	bQ	bQ
AE104	EDTA	bQ	18 ± 2	7 ± 3	11 ± 5	59 ± 12	bQ	bQ	bQ
AE104	MetalMix	37.9 ± 8.7	61 ± 16	59 ± 18	2035 ± 2251	92 ± 9	2034 ± 1 933	236 ± 233	21 ± 11
Chal. Conc., (µM)^b	7	7	9	120	5	849	3	0.18
		Metals not present in the MultiTox metal mix, but present in the mineral salts medium used (TMM)
Strain	condition metal	10⁶ Mg	10³ Ca	Mn	10³ Fe	Mo
CH34	No addition	10.7 ± 1.2	212 ± 92	531 ± 157	735 ± 80	2488 ± 359
CH34	EDTA	9.6 ± 0.3	117 ± 23	680 ± 295	636 ± 60	2117 ± 100
CH34	MetalMix	9.0 ± 0.5	213 ± 75	833 ± 311	609 ± 61	922 ± 41
AE104	No addition	10.0 ± 0.9	164 ± 17	464 ± 175	740 ± 36	2670 ± 272
AE104	EDTA	9.4 ± 0.4	154 ± 21	328 ± 134	675 ± 48	2378 ± 100
AE104	MetalMix	10.0 ± 0.5	153 ± 41	491 ± 340	652 ± 55	2222 ± 48

Table 2.

Open in new tab

Metal composition of the challenged cells as determined by ICP-MS^a.

		Metals present in the strain-specific MultiTox metal mix
Strain	condition Metal/cell	10³ Cr	10³ Co	10³ Ni	10³ Cu	10³ Zn	10³ As	10³ Cd	10³ Hg
CH34	No addition	4.8 ± 3.5	27 ± 10	20 ± 12	19 ± 10	83 ± 10	bQ	bQ	bQ
CH34	EDTA	bQ	8 ± 4	5 ± 3	7 ± 3	50 ± 12	bQ	bQ	bQ
CH34	MetalMix	75.6 ± 29.2	727 ± 278	1343 ± 338	1587 ± 543	3558 ± 2283	3746 ± 1 380	85 ± 53	27 ± 6
Chal. Conc., (µM)^b		19	347	761	241	461	1503	15	0.37
AE104	no addition	bQ	27 ± 2	7 ± 3	10 ± 2	70 ± 16	bQ	bQ	bQ
AE104	EDTA	bQ	18 ± 2	7 ± 3	11 ± 5	59 ± 12	bQ	bQ	bQ
AE104	MetalMix	37.9 ± 8.7	61 ± 16	59 ± 18	2035 ± 2251	92 ± 9	2034 ± 1 933	236 ± 233	21 ± 11
Chal. Conc., (µM)^b		7	7	9	120	5	849	3	0.18
		Metals not present in the MultiTox metal mix, but present in the mineral salts medium used (TMM)
Strain	condition metal	10⁶ Mg	10³ Ca	Mn	10³ Fe	Mo
CH34	No addition	10.7 ± 1.2	212 ± 92	531 ± 157	735 ± 80	2488 ± 359
CH34	EDTA	9.6 ± 0.3	117 ± 23	680 ± 295	636 ± 60	2117 ± 100
CH34	MetalMix	9.0 ± 0.5	213 ± 75	833 ± 311	609 ± 61	922 ± 41
AE104	No addition	10.0 ± 0.9	164 ± 17	464 ± 175	740 ± 36	2670 ± 272
AE104	EDTA	9.4 ± 0.4	154 ± 21	328 ± 134	675 ± 48	2378 ± 100
AE104	MetalMix	10.0 ± 0.5	153 ± 41	491 ± 340	652 ± 55	2222 ± 48

		Metals present in the strain-specific MultiTox metal mix
Strain	condition Metal/cell	10³ Cr	10³ Co	10³ Ni	10³ Cu	10³ Zn	10³ As	10³ Cd	10³ Hg
CH34	No addition	4.8 ± 3.5	27 ± 10	20 ± 12	19 ± 10	83 ± 10	bQ	bQ	bQ
CH34	EDTA	bQ	8 ± 4	5 ± 3	7 ± 3	50 ± 12	bQ	bQ	bQ
CH34	MetalMix	75.6 ± 29.2	727 ± 278	1343 ± 338	1587 ± 543	3558 ± 2283	3746 ± 1 380	85 ± 53	27 ± 6
Chal. Conc., (µM)^b		19	347	761	241	461	1503	15	0.37
AE104	no addition	bQ	27 ± 2	7 ± 3	10 ± 2	70 ± 16	bQ	bQ	bQ
AE104	EDTA	bQ	18 ± 2	7 ± 3	11 ± 5	59 ± 12	bQ	bQ	bQ
AE104	MetalMix	37.9 ± 8.7	61 ± 16	59 ± 18	2035 ± 2251	92 ± 9	2034 ± 1 933	236 ± 233	21 ± 11
Chal. Conc., (µM)^b		7	7	9	120	5	849	3	0.18
		Metals not present in the MultiTox metal mix, but present in the mineral salts medium used (TMM)
Strain	condition metal	10⁶ Mg	10³ Ca	Mn	10³ Fe	Mo
CH34	No addition	10.7 ± 1.2	212 ± 92	531 ± 157	735 ± 80	2488 ± 359
CH34	EDTA	9.6 ± 0.3	117 ± 23	680 ± 295	636 ± 60	2117 ± 100
CH34	MetalMix	9.0 ± 0.5	213 ± 75	833 ± 311	609 ± 61	922 ± 41
AE104	No addition	10.0 ± 0.9	164 ± 17	464 ± 175	740 ± 36	2670 ± 272
AE104	EDTA	9.4 ± 0.4	154 ± 21	328 ± 134	675 ± 48	2378 ± 100
AE104	MetalMix	10.0 ± 0.5	153 ± 41	491 ± 340	652 ± 55	2222 ± 48

^aCupriavidus metallidurans strains CH34 and AE104 were challenged with 50 µM EDTA, 3.35 mM of the CH34-specific, or 1 mM of the AE104-specific MultiTox metal mix. The metal content of the cells was determined by ICP-MS (n > 3). Bold faced letters indicate a significant (D > 1) up- and letters in italics a significant down-regulation compared to the unchallenged cells of the same strain; bQ, below limit of quantification (LOQ). ^bConcentration of an individual metal ion in the strain-specific metal mix. Please refer to the methods for details.

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