Table 2 Open in new tab GSEA analysis...

KEGG pathway	P-value	FDR	NES	Top genes
Upregulated
Extracellular matrix receptor interaction	0.0001	0.107	1.82	ITGA5, LAMA3, COL6A6
Adherens junction	0.0001	0.072	1.79	IGF1R, LMO7, FER
Neuroactive ligand receptor interaction	0.0001	0.083	1.72	CHRNG, CYSLTR1, P2RX7, CHRND
P53 signalling pathway	0.0001	0.094	1.67	SESN3, MDM4, CCNE2
Viral myocarditis	0.016	0.208	1.54	HLA-DMB, ICAM1, HLA-DMA
Cell adhesion molecules	0.023	0.463	1.39	SIGLEC1, HLA-DMB, ICAM1
Nod-like receptor signalling pathway	0.030	0.476	1.36	NOD1, CARD8, NAIP
Axon guidance	0.049	0.462	1.31	NFATC4, SEMA6A, SEMA3G
Focal adhesion	0.0001	0.531	1.23	IGF1R, HGF, MYLK
Downregulated
Ribosome	0.0001	0.0001	−3.11	RPS15A, RPS26, FAU
Oxidative phosphorylation	0.0001	0.0001	−2.87	NDUFB3, COX7B, ATP5MC1
Proteasome	0.0001	0.0001	−2.13	POMP, PSMF1, PSMB6
Citric acid cycle	0.0001	0.0001	−2.01	SDHB, SDHD, SUCLG1
RNA polymerase	0.0001	0.0001	−2.00	POLR3K, POLR2K, POL2RL
Glycine, serine and threonine metabolism	0.0001	0.001	−1.91	PHGDH, SHMT1, CTH
Gluthatione metabolism	0.0001	0.001	−1.90	GSTM5, GPX4, IDH2
Glycolysis–gluconeogenesis	0.0001	0.002	−1.89	ENO3, BPGM, ALDOA
Drug metabolism cytochrome P450	0.001	0.003	−1.85	GSTM5, GSTO1, GSTM4
Pyruvate metabolism	0.001	0.003	−1.84	LDHD, PC, MDH2
Lysosome	0.0001	0.003	−1.83	ATP6V0B, LAPTM4A, DNASE2
Valine, leucine and isoleucine degradation	0.001	0.004	−1.80	ECHS1, HSD17B10, BCKDHA
Glycosilphosphatidylinositol biosynthesis	0.01	0.016	−1.68	PIGP, DPM2, PIGZ
Protein export	0.004	0.022	−1.64	SPCS1, SRP14, SRP9
Cysteine metabolism	0.04	0.086	−1.49	GOT2, ADI1, CTH

KEGG pathway

P-value

FDR

NES

Top genes

Upregulated

Extracellular matrix receptor interaction

0.0001

0.107

1.82

ITGA5, LAMA3, COL6A6

Adherens junction

0.0001

0.072

1.79

IGF1R, LMO7, FER

Neuroactive ligand receptor interaction

0.0001

0.083

1.72

CHRNG, CYSLTR1, P2RX7, CHRND

P53 signalling pathway

0.0001

0.094

1.67

SESN3, MDM4, CCNE2

Viral myocarditis

0.016

0.208

1.54

HLA-DMB, ICAM1, HLA-DMA

Cell adhesion molecules

0.023

0.463

1.39

SIGLEC1, HLA-DMB, ICAM1

Nod-like receptor signalling pathway

0.030

0.476

1.36

NOD1, CARD8, NAIP

Axon guidance

0.049

0.462

1.31

NFATC4, SEMA6A, SEMA3G

Focal adhesion

0.0001

0.531

1.23

IGF1R, HGF, MYLK

Downregulated

Ribosome

0.0001

−3.11

RPS15A, RPS26, FAU

Oxidative phosphorylation

0.0001

−2.87

NDUFB3, COX7B, ATP5MC1

Proteasome

0.0001

−2.13

POMP, PSMF1, PSMB6

Citric acid cycle

0.0001

−2.01

SDHB, SDHD, SUCLG1

RNA polymerase

0.0001

−2.00

POLR3K, POLR2K, POL2RL

Glycine, serine and threonine metabolism

0.0001

0.001

−1.91

PHGDH, SHMT1, CTH

Gluthatione metabolism

0.0001

0.001

−1.90

GSTM5, GPX4, IDH2

Glycolysis–gluconeogenesis

0.0001

0.002

−1.89

ENO3, BPGM, ALDOA

Drug metabolism cytochrome P450

0.001

0.003

−1.85

GSTM5, GSTO1, GSTM4

Pyruvate metabolism

0.001

0.003

−1.84

LDHD, PC, MDH2

Lysosome

0.0001

0.003

−1.83

ATP6V0B, LAPTM4A, DNASE2

Valine, leucine and isoleucine degradation

0.001

0.004

−1.80

ECHS1, HSD17B10, BCKDHA

Glycosilphosphatidylinositol biosynthesis

0.01

0.016

−1.68

PIGP, DPM2, PIGZ

Protein export

0.004

0.022

−1.64

SPCS1, SRP14, SRP9

Cysteine metabolism

0.04

0.086

−1.49

GOT2, ADI1, CTH

KEGG pathway	P-value	FDR	NES	Top genes
Upregulated
Extracellular matrix receptor interaction	0.0001	0.107	1.82	ITGA5, LAMA3, COL6A6
Adherens junction	0.0001	0.072	1.79	IGF1R, LMO7, FER
Neuroactive ligand receptor interaction	0.0001	0.083	1.72	CHRNG, CYSLTR1, P2RX7, CHRND
P53 signalling pathway	0.0001	0.094	1.67	SESN3, MDM4, CCNE2
Viral myocarditis	0.016	0.208	1.54	HLA-DMB, ICAM1, HLA-DMA
Cell adhesion molecules	0.023	0.463	1.39	SIGLEC1, HLA-DMB, ICAM1
Nod-like receptor signalling pathway	0.030	0.476	1.36	NOD1, CARD8, NAIP
Axon guidance	0.049	0.462	1.31	NFATC4, SEMA6A, SEMA3G
Focal adhesion	0.0001	0.531	1.23	IGF1R, HGF, MYLK
Downregulated
Ribosome	0.0001	0.0001	−3.11	RPS15A, RPS26, FAU
Oxidative phosphorylation	0.0001	0.0001	−2.87	NDUFB3, COX7B, ATP5MC1
Proteasome	0.0001	0.0001	−2.13	POMP, PSMF1, PSMB6
Citric acid cycle	0.0001	0.0001	−2.01	SDHB, SDHD, SUCLG1
RNA polymerase	0.0001	0.0001	−2.00	POLR3K, POLR2K, POL2RL
Glycine, serine and threonine metabolism	0.0001	0.001	−1.91	PHGDH, SHMT1, CTH
Gluthatione metabolism	0.0001	0.001	−1.90	GSTM5, GPX4, IDH2
Glycolysis–gluconeogenesis	0.0001	0.002	−1.89	ENO3, BPGM, ALDOA
Drug metabolism cytochrome P450	0.001	0.003	−1.85	GSTM5, GSTO1, GSTM4
Pyruvate metabolism	0.001	0.003	−1.84	LDHD, PC, MDH2
Lysosome	0.0001	0.003	−1.83	ATP6V0B, LAPTM4A, DNASE2
Valine, leucine and isoleucine degradation	0.001	0.004	−1.80	ECHS1, HSD17B10, BCKDHA
Glycosilphosphatidylinositol biosynthesis	0.01	0.016	−1.68	PIGP, DPM2, PIGZ
Protein export	0.004	0.022	−1.64	SPCS1, SRP14, SRP9
Cysteine metabolism	0.04	0.086	−1.49	GOT2, ADI1, CTH

Table 2

Open in new tab

GSEA analysis using KEGG pathway in fast and slow myonuclei

KEGG pathway	P-value	FDR	NES	Top genes
Upregulated
Extracellular matrix receptor interaction	0.0001	0.107	1.82	ITGA5, LAMA3, COL6A6
Adherens junction	0.0001	0.072	1.79	IGF1R, LMO7, FER
Neuroactive ligand receptor interaction	0.0001	0.083	1.72	CHRNG, CYSLTR1, P2RX7, CHRND
P53 signalling pathway	0.0001	0.094	1.67	SESN3, MDM4, CCNE2
Viral myocarditis	0.016	0.208	1.54	HLA-DMB, ICAM1, HLA-DMA
Cell adhesion molecules	0.023	0.463	1.39	SIGLEC1, HLA-DMB, ICAM1
Nod-like receptor signalling pathway	0.030	0.476	1.36	NOD1, CARD8, NAIP
Axon guidance	0.049	0.462	1.31	NFATC4, SEMA6A, SEMA3G
Focal adhesion	0.0001	0.531	1.23	IGF1R, HGF, MYLK
Downregulated
Ribosome	0.0001	0.0001	−3.11	RPS15A, RPS26, FAU
Oxidative phosphorylation	0.0001	0.0001	−2.87	NDUFB3, COX7B, ATP5MC1
Proteasome	0.0001	0.0001	−2.13	POMP, PSMF1, PSMB6
Citric acid cycle	0.0001	0.0001	−2.01	SDHB, SDHD, SUCLG1
RNA polymerase	0.0001	0.0001	−2.00	POLR3K, POLR2K, POL2RL
Glycine, serine and threonine metabolism	0.0001	0.001	−1.91	PHGDH, SHMT1, CTH
Gluthatione metabolism	0.0001	0.001	−1.90	GSTM5, GPX4, IDH2
Glycolysis–gluconeogenesis	0.0001	0.002	−1.89	ENO3, BPGM, ALDOA
Drug metabolism cytochrome P450	0.001	0.003	−1.85	GSTM5, GSTO1, GSTM4
Pyruvate metabolism	0.001	0.003	−1.84	LDHD, PC, MDH2
Lysosome	0.0001	0.003	−1.83	ATP6V0B, LAPTM4A, DNASE2
Valine, leucine and isoleucine degradation	0.001	0.004	−1.80	ECHS1, HSD17B10, BCKDHA
Glycosilphosphatidylinositol biosynthesis	0.01	0.016	−1.68	PIGP, DPM2, PIGZ
Protein export	0.004	0.022	−1.64	SPCS1, SRP14, SRP9
Cysteine metabolism	0.04	0.086	−1.49	GOT2, ADI1, CTH

KEGG pathway	P-value	FDR	NES	Top genes
Upregulated
Extracellular matrix receptor interaction	0.0001	0.107	1.82	ITGA5, LAMA3, COL6A6
Adherens junction	0.0001	0.072	1.79	IGF1R, LMO7, FER
Neuroactive ligand receptor interaction	0.0001	0.083	1.72	CHRNG, CYSLTR1, P2RX7, CHRND
P53 signalling pathway	0.0001	0.094	1.67	SESN3, MDM4, CCNE2
Viral myocarditis	0.016	0.208	1.54	HLA-DMB, ICAM1, HLA-DMA
Cell adhesion molecules	0.023	0.463	1.39	SIGLEC1, HLA-DMB, ICAM1
Nod-like receptor signalling pathway	0.030	0.476	1.36	NOD1, CARD8, NAIP
Axon guidance	0.049	0.462	1.31	NFATC4, SEMA6A, SEMA3G
Focal adhesion	0.0001	0.531	1.23	IGF1R, HGF, MYLK
Downregulated
Ribosome	0.0001	0.0001	−3.11	RPS15A, RPS26, FAU
Oxidative phosphorylation	0.0001	0.0001	−2.87	NDUFB3, COX7B, ATP5MC1
Proteasome	0.0001	0.0001	−2.13	POMP, PSMF1, PSMB6
Citric acid cycle	0.0001	0.0001	−2.01	SDHB, SDHD, SUCLG1
RNA polymerase	0.0001	0.0001	−2.00	POLR3K, POLR2K, POL2RL
Glycine, serine and threonine metabolism	0.0001	0.001	−1.91	PHGDH, SHMT1, CTH
Gluthatione metabolism	0.0001	0.001	−1.90	GSTM5, GPX4, IDH2
Glycolysis–gluconeogenesis	0.0001	0.002	−1.89	ENO3, BPGM, ALDOA
Drug metabolism cytochrome P450	0.001	0.003	−1.85	GSTM5, GSTO1, GSTM4
Pyruvate metabolism	0.001	0.003	−1.84	LDHD, PC, MDH2
Lysosome	0.0001	0.003	−1.83	ATP6V0B, LAPTM4A, DNASE2
Valine, leucine and isoleucine degradation	0.001	0.004	−1.80	ECHS1, HSD17B10, BCKDHA
Glycosilphosphatidylinositol biosynthesis	0.01	0.016	−1.68	PIGP, DPM2, PIGZ
Protein export	0.004	0.022	−1.64	SPCS1, SRP14, SRP9
Cysteine metabolism	0.04	0.086	−1.49	GOT2, ADI1, CTH

FDR = false discovery rate; GSEA = gene set enrichment analysis; NES = normalized enrichment score.

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