Table 3 Open in new tab Detailed data...

Sample	HBCC	HBCS
30.0 °C Water + CUR	33 ± 2.35^a	19 ± 1.87^bc	15 ± 1.79^c	149 ± 2.18^bc	116 ± 6.04^c	115 ± 6.59^d
40.0 °C Water + CUR	26 ± 1.92^b	21 ± 1.64^b	17 ± 1.92^bc	156 ± 6.49^ab	118 ± 4.15^bc	130 ± 6.54^c
50.0 °C Water + CUR	19 ± 1.79^c	17 ± 1.50^c	16 ± 2.17^c	163 ± 5.96^a	126 ± 6.50^ab	150 ± 7.51^ab
60.0 °C Water + CUR	20 ± 2.35^c	17 ± 2.28^bc	19 ± 2.05^bc	147 ± 4.21^c	130 ± 7.15^a	159 ± 3.54^a
70.0 °C Water + CUR	26 ± 3.11^b	26 ± 1.70^a	25 ± 3.09^a	138 ± 2.68^d	130 ± 5.02^a	141 ± 6.84^b
80.0 °C Water + CUR	21 ± 1.48^c	13 ± 2.36^d	21 ± 1.64^b	137 ± 5.93^d	131 ± 2.45^a	151 ± 4.32^ab
90.0 °C Water + CUR	17 ± 1.58^c	17 ± 2.49^c	17 ± 2.74^bc	132 ± 3.81^d	132 ± 6.02^a	156 ± 6.34^a

Sample	HBCC	HBCS
30.0 °C Water + CUR	33 ± 2.35^a	19 ± 1.87^bc	15 ± 1.79^c	149 ± 2.18^bc	116 ± 6.04^c	115 ± 6.59^d
40.0 °C Water + CUR	26 ± 1.92^b	21 ± 1.64^b	17 ± 1.92^bc	156 ± 6.49^ab	118 ± 4.15^bc	130 ± 6.54^c
50.0 °C Water + CUR	19 ± 1.79^c	17 ± 1.50^c	16 ± 2.17^c	163 ± 5.96^a	126 ± 6.50^ab	150 ± 7.51^ab
60.0 °C Water + CUR	20 ± 2.35^c	17 ± 2.28^bc	19 ± 2.05^bc	147 ± 4.21^c	130 ± 7.15^a	159 ± 3.54^a
70.0 °C Water + CUR	26 ± 3.11^b	26 ± 1.70^a	25 ± 3.09^a	138 ± 2.68^d	130 ± 5.02^a	141 ± 6.84^b
80.0 °C Water + CUR	21 ± 1.48^c	13 ± 2.36^d	21 ± 1.64^b	137 ± 5.93^d	131 ± 2.45^a	151 ± 4.32^ab
90.0 °C Water + CUR	17 ± 1.58^c	17 ± 2.49^c	17 ± 2.74^bc	132 ± 3.81^d	132 ± 6.02^a	156 ± 6.34^a

Table 3

Detailed data for the strong, medium, and weak hydrogen bonds of HBCC and HBCS

Sample	HBCC			HBCS
Sample	Strong	Medium	Weak	Strong	Medium	Weak
30.0 °C Water + CUR	33 ± 2.35^a	19 ± 1.87^bc	15 ± 1.79^c	149 ± 2.18^bc	116 ± 6.04^c	115 ± 6.59^d
40.0 °C Water + CUR	26 ± 1.92^b	21 ± 1.64^b	17 ± 1.92^bc	156 ± 6.49^ab	118 ± 4.15^bc	130 ± 6.54^c
50.0 °C Water + CUR	19 ± 1.79^c	17 ± 1.50^c	16 ± 2.17^c	163 ± 5.96^a	126 ± 6.50^ab	150 ± 7.51^ab
60.0 °C Water + CUR	20 ± 2.35^c	17 ± 2.28^bc	19 ± 2.05^bc	147 ± 4.21^c	130 ± 7.15^a	159 ± 3.54^a
70.0 °C Water + CUR	26 ± 3.11^b	26 ± 1.70^a	25 ± 3.09^a	138 ± 2.68^d	130 ± 5.02^a	141 ± 6.84^b
80.0 °C Water + CUR	21 ± 1.48^c	13 ± 2.36^d	21 ± 1.64^b	137 ± 5.93^d	131 ± 2.45^a	151 ± 4.32^ab
90.0 °C Water + CUR	17 ± 1.58^c	17 ± 2.49^c	17 ± 2.74^bc	132 ± 3.81^d	132 ± 6.02^a	156 ± 6.34^a

Sample	HBCC			HBCS
Sample	Strong	Medium	Weak	Strong	Medium	Weak
30.0 °C Water + CUR	33 ± 2.35^a	19 ± 1.87^bc	15 ± 1.79^c	149 ± 2.18^bc	116 ± 6.04^c	115 ± 6.59^d
40.0 °C Water + CUR	26 ± 1.92^b	21 ± 1.64^b	17 ± 1.92^bc	156 ± 6.49^ab	118 ± 4.15^bc	130 ± 6.54^c
50.0 °C Water + CUR	19 ± 1.79^c	17 ± 1.50^c	16 ± 2.17^c	163 ± 5.96^a	126 ± 6.50^ab	150 ± 7.51^ab
60.0 °C Water + CUR	20 ± 2.35^c	17 ± 2.28^bc	19 ± 2.05^bc	147 ± 4.21^c	130 ± 7.15^a	159 ± 3.54^a
70.0 °C Water + CUR	26 ± 3.11^b	26 ± 1.70^a	25 ± 3.09^a	138 ± 2.68^d	130 ± 5.02^a	141 ± 6.84^b
80.0 °C Water + CUR	21 ± 1.48^c	13 ± 2.36^d	21 ± 1.64^b	137 ± 5.93^d	131 ± 2.45^a	151 ± 4.32^ab
90.0 °C Water + CUR	17 ± 1.58^c	17 ± 2.49^c	17 ± 2.74^bc	132 ± 3.81^d	132 ± 6.02^a	156 ± 6.34^a

HBCC, hydrogen bonds between curdlan; HBCS, hydrogen bonds between curdlan and water; Medium, RDF truncation distance 1.875–2.235 Å; Strong, RDF truncation distance 1.385–1.875 Å; Water + CUR, simulation system determined by binding energy; Weak, RDF truncation distance 2.235–2.995 Å. The means (n > 3) of the superscript letters (a–d) in a given column are found to be statistically different (P < 0.05).

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