Table 2 Open in new tab Effect of...

	Control	Black tea	Green tea
β-sheet/%	36.07 ± 0.32^d	43.03 ± 0.70^c	43.95 ± 0.38^b	51.35 ± 0.43^a	50.78 ± 0.44^a	50.87 ± 0.25^a	42.64 ± 0.31^c	42.90 ± 0.53^c	50.48 ± 1.05^a	49.34 ± 1.02^ab	49.01 ± 0.53^b
α-helix/%	25.50 ± 0.18^a	18.49 ± 0.21^b	18.21 ± 0.10^b	16.00 ± 0.20^c	16.05 ± 0.14^c	16.04 ± 0.08^c	18.65 ± 0.08^b	18.53 ± 0.16^b	16.09 ± 0.28^c	16.37 ± 0.29^c	16.49 ± 0.16^c
β-turn/%	24.34 ± 0.83^a	24.84 ± 0.44^a	24.28 ± 0.24^a	19.66 ± 0.45^b	19.63 ± 0.23^b	19.57 ± 0.16^b	25.05 ± 0.19^a	24.94 ± 0.34^a	19.80 ± 0.62^b	20.43 ± 0.50^b	20.65 ± 0.30^b
Random coil/%	13.49 ± 0.01^b	13.65 ± 0.07^a	13.56 ± 0.04^ab	13.61 ± 0.08^ab	13.57 ± 0.08^ab	13.56 ± 0.02^ab	13.66 ± 0.04^ab	13.64 ± 0.04^ab	13.62 ± 0.14^ab	13.86 ± 0.23^a	13.85 ± 0.08^a
SA	28.63 ± 0.29^a	27.16 ± 0.09^bc	26.99 ± 0.25^c	25.00 ± 0.12^d	27.44 ± 0.19^bc	27.52 ± 0.15^b	28.56 ± 0.41^ab	27.67 ± 0.24^b	25.76 ± 0.05^c	28.27 ± 0.01^ab	28.79 ± 0.71^a
SB	20.22 ± 0.24^a	16.73 ± 0.26^b	16.32 ± 0.58^b	14.89 ± 0.12^c	14.92 ± 0.09^c	14.41 ± 0.31^c	18.09 ± 0.25^b	17.84 ± 0.09^b	15.94 ± 0.06^c	15.94 ± 0.13^c	14.66 ± 0.36^d
SC	32.65 ± 0.39^a	26.55 ± 0.60^b	25.93 ± 0.08^bc	25.10 ± 0.02^cd	24.60 ± 0.35^d	24.52 ± 0.43^d	29.72 ± 0.00^b	29.13 ± 0.28^c	28.17 ± 0.05^d	28.64 ± 0.05^cd	28.63 ± 0.11^cd
SD	18.51 ± 0.44^f	28.56 ± 0.42^e	30.75 ± 0.25^c	35.00 ± 0.02^a	33.04 ± 0.26^b	33.55 ± 0.58^b	23.63 ± 0.15^e	25.35 ± 0.44^d	30.13 ± 0.04^a	27.15 ± 0.17^c	27.97 ± 0.39^b

Control

Black tea

Green tea

β-sheet/%

36.07 ± 0.32^d

43.03 ± 0.70^c

43.95 ± 0.38^b

51.35 ± 0.43^a

50.78 ± 0.44^a

50.87 ± 0.25^a

42.64 ± 0.31^c

42.90 ± 0.53^c

50.48 ± 1.05^a

49.34 ± 1.02^ab

49.01 ± 0.53^b

α-helix/%

25.50 ± 0.18^a

18.49 ± 0.21^b

18.21 ± 0.10^b

16.00 ± 0.20^c

16.05 ± 0.14^c

16.04 ± 0.08^c

18.65 ± 0.08^b

18.53 ± 0.16^b

16.09 ± 0.28^c

16.37 ± 0.29^c

16.49 ± 0.16^c

β-turn/%

24.34 ± 0.83^a

24.84 ± 0.44^a

24.28 ± 0.24^a

19.66 ± 0.45^b

19.63 ± 0.23^b

19.57 ± 0.16^b

25.05 ± 0.19^a

24.94 ± 0.34^a

19.80 ± 0.62^b

20.43 ± 0.50^b

20.65 ± 0.30^b

Random coil/%

13.49 ± 0.01^b

13.65 ± 0.07^a

13.56 ± 0.04^ab

13.61 ± 0.08^ab

13.57 ± 0.08^ab

13.56 ± 0.02^ab

13.66 ± 0.04^ab

13.64 ± 0.04^ab

13.62 ± 0.14^ab

13.86 ± 0.23^a

13.85 ± 0.08^a

28.63 ± 0.29^a

27.16 ± 0.09^bc

26.99 ± 0.25^c

25.00 ± 0.12^d

27.44 ± 0.19^bc

27.52 ± 0.15^b

28.56 ± 0.41^ab

27.67 ± 0.24^b

25.76 ± 0.05^c

28.27 ± 0.01^ab

28.79 ± 0.71^a

20.22 ± 0.24^a

16.73 ± 0.26^b

16.32 ± 0.58^b

14.89 ± 0.12^c

14.92 ± 0.09^c

14.41 ± 0.31^c

18.09 ± 0.25^b

17.84 ± 0.09^b

15.94 ± 0.06^c

15.94 ± 0.13^c

14.66 ± 0.36^d

32.65 ± 0.39^a

26.55 ± 0.60^b

25.93 ± 0.08^bc

25.10 ± 0.02^cd

24.60 ± 0.35^d

24.52 ± 0.43^d

29.72 ± 0.00^b

29.13 ± 0.28^c

28.17 ± 0.05^d

28.64 ± 0.05^cd

28.63 ± 0.11^cd

18.51 ± 0.44^f

28.56 ± 0.42^e

30.75 ± 0.25^c

35.00 ± 0.02^a

33.04 ± 0.26^b

33.55 ± 0.58^b

23.63 ± 0.15^e

25.35 ± 0.44^d

30.13 ± 0.04^a

27.15 ± 0.17^c

27.97 ± 0.39^b

	Control	Black tea	Green tea
β-sheet/%	36.07 ± 0.32^d	43.03 ± 0.70^c	43.95 ± 0.38^b	51.35 ± 0.43^a	50.78 ± 0.44^a	50.87 ± 0.25^a	42.64 ± 0.31^c	42.90 ± 0.53^c	50.48 ± 1.05^a	49.34 ± 1.02^ab	49.01 ± 0.53^b
α-helix/%	25.50 ± 0.18^a	18.49 ± 0.21^b	18.21 ± 0.10^b	16.00 ± 0.20^c	16.05 ± 0.14^c	16.04 ± 0.08^c	18.65 ± 0.08^b	18.53 ± 0.16^b	16.09 ± 0.28^c	16.37 ± 0.29^c	16.49 ± 0.16^c
β-turn/%	24.34 ± 0.83^a	24.84 ± 0.44^a	24.28 ± 0.24^a	19.66 ± 0.45^b	19.63 ± 0.23^b	19.57 ± 0.16^b	25.05 ± 0.19^a	24.94 ± 0.34^a	19.80 ± 0.62^b	20.43 ± 0.50^b	20.65 ± 0.30^b
Random coil/%	13.49 ± 0.01^b	13.65 ± 0.07^a	13.56 ± 0.04^ab	13.61 ± 0.08^ab	13.57 ± 0.08^ab	13.56 ± 0.02^ab	13.66 ± 0.04^ab	13.64 ± 0.04^ab	13.62 ± 0.14^ab	13.86 ± 0.23^a	13.85 ± 0.08^a
SA	28.63 ± 0.29^a	27.16 ± 0.09^bc	26.99 ± 0.25^c	25.00 ± 0.12^d	27.44 ± 0.19^bc	27.52 ± 0.15^b	28.56 ± 0.41^ab	27.67 ± 0.24^b	25.76 ± 0.05^c	28.27 ± 0.01^ab	28.79 ± 0.71^a
SB	20.22 ± 0.24^a	16.73 ± 0.26^b	16.32 ± 0.58^b	14.89 ± 0.12^c	14.92 ± 0.09^c	14.41 ± 0.31^c	18.09 ± 0.25^b	17.84 ± 0.09^b	15.94 ± 0.06^c	15.94 ± 0.13^c	14.66 ± 0.36^d
SC	32.65 ± 0.39^a	26.55 ± 0.60^b	25.93 ± 0.08^bc	25.10 ± 0.02^cd	24.60 ± 0.35^d	24.52 ± 0.43^d	29.72 ± 0.00^b	29.13 ± 0.28^c	28.17 ± 0.05^d	28.64 ± 0.05^cd	28.63 ± 0.11^cd
SD	18.51 ± 0.44^f	28.56 ± 0.42^e	30.75 ± 0.25^c	35.00 ± 0.02^a	33.04 ± 0.26^b	33.55 ± 0.58^b	23.63 ± 0.15^e	25.35 ± 0.44^d	30.13 ± 0.04^a	27.15 ± 0.17^c	27.97 ± 0.39^b

Table 2

Open in new tab

Effect of alkali-induced tea water extract on the secondary structure and protein interaction force of EWGs

	Control	Black tea					Green tea
	Control	0	6	12	24	48	0	6	12	24	48
β-sheet/%	36.07 ± 0.32^d	43.03 ± 0.70^c	43.95 ± 0.38^b	51.35 ± 0.43^a	50.78 ± 0.44^a	50.87 ± 0.25^a	42.64 ± 0.31^c	42.90 ± 0.53^c	50.48 ± 1.05^a	49.34 ± 1.02^ab	49.01 ± 0.53^b
α-helix/%	25.50 ± 0.18^a	18.49 ± 0.21^b	18.21 ± 0.10^b	16.00 ± 0.20^c	16.05 ± 0.14^c	16.04 ± 0.08^c	18.65 ± 0.08^b	18.53 ± 0.16^b	16.09 ± 0.28^c	16.37 ± 0.29^c	16.49 ± 0.16^c
β-turn/%	24.34 ± 0.83^a	24.84 ± 0.44^a	24.28 ± 0.24^a	19.66 ± 0.45^b	19.63 ± 0.23^b	19.57 ± 0.16^b	25.05 ± 0.19^a	24.94 ± 0.34^a	19.80 ± 0.62^b	20.43 ± 0.50^b	20.65 ± 0.30^b
Random coil/%	13.49 ± 0.01^b	13.65 ± 0.07^a	13.56 ± 0.04^ab	13.61 ± 0.08^ab	13.57 ± 0.08^ab	13.56 ± 0.02^ab	13.66 ± 0.04^ab	13.64 ± 0.04^ab	13.62 ± 0.14^ab	13.86 ± 0.23^a	13.85 ± 0.08^a
SA	28.63 ± 0.29^a	27.16 ± 0.09^bc	26.99 ± 0.25^c	25.00 ± 0.12^d	27.44 ± 0.19^bc	27.52 ± 0.15^b	28.56 ± 0.41^ab	27.67 ± 0.24^b	25.76 ± 0.05^c	28.27 ± 0.01^ab	28.79 ± 0.71^a
SB	20.22 ± 0.24^a	16.73 ± 0.26^b	16.32 ± 0.58^b	14.89 ± 0.12^c	14.92 ± 0.09^c	14.41 ± 0.31^c	18.09 ± 0.25^b	17.84 ± 0.09^b	15.94 ± 0.06^c	15.94 ± 0.13^c	14.66 ± 0.36^d
SC	32.65 ± 0.39^a	26.55 ± 0.60^b	25.93 ± 0.08^bc	25.10 ± 0.02^cd	24.60 ± 0.35^d	24.52 ± 0.43^d	29.72 ± 0.00^b	29.13 ± 0.28^c	28.17 ± 0.05^d	28.64 ± 0.05^cd	28.63 ± 0.11^cd
SD	18.51 ± 0.44^f	28.56 ± 0.42^e	30.75 ± 0.25^c	35.00 ± 0.02^a	33.04 ± 0.26^b	33.55 ± 0.58^b	23.63 ± 0.15^e	25.35 ± 0.44^d	30.13 ± 0.04^a	27.15 ± 0.17^c	27.97 ± 0.39^b

	Control	Black tea					Green tea
	Control	0	6	12	24	48	0	6	12	24	48
β-sheet/%	36.07 ± 0.32^d	43.03 ± 0.70^c	43.95 ± 0.38^b	51.35 ± 0.43^a	50.78 ± 0.44^a	50.87 ± 0.25^a	42.64 ± 0.31^c	42.90 ± 0.53^c	50.48 ± 1.05^a	49.34 ± 1.02^ab	49.01 ± 0.53^b
α-helix/%	25.50 ± 0.18^a	18.49 ± 0.21^b	18.21 ± 0.10^b	16.00 ± 0.20^c	16.05 ± 0.14^c	16.04 ± 0.08^c	18.65 ± 0.08^b	18.53 ± 0.16^b	16.09 ± 0.28^c	16.37 ± 0.29^c	16.49 ± 0.16^c
β-turn/%	24.34 ± 0.83^a	24.84 ± 0.44^a	24.28 ± 0.24^a	19.66 ± 0.45^b	19.63 ± 0.23^b	19.57 ± 0.16^b	25.05 ± 0.19^a	24.94 ± 0.34^a	19.80 ± 0.62^b	20.43 ± 0.50^b	20.65 ± 0.30^b
Random coil/%	13.49 ± 0.01^b	13.65 ± 0.07^a	13.56 ± 0.04^ab	13.61 ± 0.08^ab	13.57 ± 0.08^ab	13.56 ± 0.02^ab	13.66 ± 0.04^ab	13.64 ± 0.04^ab	13.62 ± 0.14^ab	13.86 ± 0.23^a	13.85 ± 0.08^a
SA	28.63 ± 0.29^a	27.16 ± 0.09^bc	26.99 ± 0.25^c	25.00 ± 0.12^d	27.44 ± 0.19^bc	27.52 ± 0.15^b	28.56 ± 0.41^ab	27.67 ± 0.24^b	25.76 ± 0.05^c	28.27 ± 0.01^ab	28.79 ± 0.71^a
SB	20.22 ± 0.24^a	16.73 ± 0.26^b	16.32 ± 0.58^b	14.89 ± 0.12^c	14.92 ± 0.09^c	14.41 ± 0.31^c	18.09 ± 0.25^b	17.84 ± 0.09^b	15.94 ± 0.06^c	15.94 ± 0.13^c	14.66 ± 0.36^d
SC	32.65 ± 0.39^a	26.55 ± 0.60^b	25.93 ± 0.08^bc	25.10 ± 0.02^cd	24.60 ± 0.35^d	24.52 ± 0.43^d	29.72 ± 0.00^b	29.13 ± 0.28^c	28.17 ± 0.05^d	28.64 ± 0.05^cd	28.63 ± 0.11^cd
SD	18.51 ± 0.44^f	28.56 ± 0.42^e	30.75 ± 0.25^c	35.00 ± 0.02^a	33.04 ± 0.26^b	33.55 ± 0.58^b	23.63 ± 0.15^e	25.35 ± 0.44^d	30.13 ± 0.04^a	27.15 ± 0.17^c	27.97 ± 0.39^b

Lowercase letters indicate significant differences between the same and control samples (P < 0.05).

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