Percent infection by CLas in different Diaphorina citri tissues as measured by qPCR, and the mean number of RNAseq reads that aligned to the CLas genome (psy62) from each dataset
| Dataset . | Mean CLas reads 4 . | % Infection5 . | |
|---|---|---|---|
| Midgut1 | CLas (−) | 1 | 0 |
| CLas (+) | 212 | 82 | |
| Salivary gland2 | CLas (−) | 0.5 | 0 |
| CLas (+) | 1,965.5 | 73 | |
| Bacteriome3 | CLas (−) | 0.8 | 0 |
| CLas (+) | 3.4 | 85 | |
| Head3 | CLas (−) | 174.6 | 0 |
| CLas (+) | 2,681.8 | 85 | |
| Dataset . | Mean CLas reads 4 . | % Infection5 . | |
|---|---|---|---|
| Midgut1 | CLas (−) | 1 | 0 |
| CLas (+) | 212 | 82 | |
| Salivary gland2 | CLas (−) | 0.5 | 0 |
| CLas (+) | 1,965.5 | 73 | |
| Bacteriome3 | CLas (−) | 0.8 | 0 |
| CLas (+) | 3.4 | 85 | |
| Head3 | CLas (−) | 174.6 | 0 |
| CLas (+) | 2,681.8 | 85 | |
qPCR Cq data from Kruse et al. [16]; reads aligning to CLas are from our own alignments.
Salivary glands from a colony with a high (>90%) infection rate.
Bacteriomes and heads were taken from the same insects, and whole insects were used for qPCR of CLas titer, so the mean Cq value is the same for both datasets.
Low read counts may represent sequences from contaminating CLas sequences remaining within the D. citri genome (which need to be removed), or sequences transferred to D. citri, or found in common in other bacterial symbionts present.
Cq values of 40 translate to 0 titer of the target bacterium. Cq values are calculated using 20–30 whole-body individuals from each parent colony of each dataset. All Cq <40 are counted for percent infection.
Percent infection by CLas in different Diaphorina citri tissues as measured by qPCR, and the mean number of RNAseq reads that aligned to the CLas genome (psy62) from each dataset
| Dataset . | Mean CLas reads 4 . | % Infection5 . | |
|---|---|---|---|
| Midgut1 | CLas (−) | 1 | 0 |
| CLas (+) | 212 | 82 | |
| Salivary gland2 | CLas (−) | 0.5 | 0 |
| CLas (+) | 1,965.5 | 73 | |
| Bacteriome3 | CLas (−) | 0.8 | 0 |
| CLas (+) | 3.4 | 85 | |
| Head3 | CLas (−) | 174.6 | 0 |
| CLas (+) | 2,681.8 | 85 | |
| Dataset . | Mean CLas reads 4 . | % Infection5 . | |
|---|---|---|---|
| Midgut1 | CLas (−) | 1 | 0 |
| CLas (+) | 212 | 82 | |
| Salivary gland2 | CLas (−) | 0.5 | 0 |
| CLas (+) | 1,965.5 | 73 | |
| Bacteriome3 | CLas (−) | 0.8 | 0 |
| CLas (+) | 3.4 | 85 | |
| Head3 | CLas (−) | 174.6 | 0 |
| CLas (+) | 2,681.8 | 85 | |
qPCR Cq data from Kruse et al. [16]; reads aligning to CLas are from our own alignments.
Salivary glands from a colony with a high (>90%) infection rate.
Bacteriomes and heads were taken from the same insects, and whole insects were used for qPCR of CLas titer, so the mean Cq value is the same for both datasets.
Low read counts may represent sequences from contaminating CLas sequences remaining within the D. citri genome (which need to be removed), or sequences transferred to D. citri, or found in common in other bacterial symbionts present.
Cq values of 40 translate to 0 titer of the target bacterium. Cq values are calculated using 20–30 whole-body individuals from each parent colony of each dataset. All Cq <40 are counted for percent infection.
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