Metadata on each of the four datasets used in this study, specifically highlighting the ways each dataset differs from the next; following sequencing, all data were treated to the same methods
| Tissue type . | No. biological replicates . | Time at −80 °C . | No. psyllids pooled/replicate . | Colony location . | RNA extraction . |
|---|---|---|---|---|---|
| Bacteriome | 5 CLas (+), 5 CLas (−) | 6 months | 120, 120 | Ithaca, NY | Qiagen RNeasy |
| Head | 5 CLas (+), 5 CLas (−) | 6 months | 120, 120 | Ithaca, NY | Qiagen RNeasy |
| Salivary gland | 4 CLas (+), 4 CLas (−) | Replicates 1–3, 1 yearReplicates 4, 2 year | 150, 150 | Fort Pierce, FL | TRIzol |
| Midgut [16] | 3 CLas (+), 3 CLas (−) | <1 month | 250, 250 | Fort Pierce, FL | TRIzol |
| Tissue type . | No. biological replicates . | Time at −80 °C . | No. psyllids pooled/replicate . | Colony location . | RNA extraction . |
|---|---|---|---|---|---|
| Bacteriome | 5 CLas (+), 5 CLas (−) | 6 months | 120, 120 | Ithaca, NY | Qiagen RNeasy |
| Head | 5 CLas (+), 5 CLas (−) | 6 months | 120, 120 | Ithaca, NY | Qiagen RNeasy |
| Salivary gland | 4 CLas (+), 4 CLas (−) | Replicates 1–3, 1 yearReplicates 4, 2 year | 150, 150 | Fort Pierce, FL | TRIzol |
| Midgut [16] | 3 CLas (+), 3 CLas (−) | <1 month | 250, 250 | Fort Pierce, FL | TRIzol |
Metadata on each of the four datasets used in this study, specifically highlighting the ways each dataset differs from the next; following sequencing, all data were treated to the same methods
| Tissue type . | No. biological replicates . | Time at −80 °C . | No. psyllids pooled/replicate . | Colony location . | RNA extraction . |
|---|---|---|---|---|---|
| Bacteriome | 5 CLas (+), 5 CLas (−) | 6 months | 120, 120 | Ithaca, NY | Qiagen RNeasy |
| Head | 5 CLas (+), 5 CLas (−) | 6 months | 120, 120 | Ithaca, NY | Qiagen RNeasy |
| Salivary gland | 4 CLas (+), 4 CLas (−) | Replicates 1–3, 1 yearReplicates 4, 2 year | 150, 150 | Fort Pierce, FL | TRIzol |
| Midgut [16] | 3 CLas (+), 3 CLas (−) | <1 month | 250, 250 | Fort Pierce, FL | TRIzol |
| Tissue type . | No. biological replicates . | Time at −80 °C . | No. psyllids pooled/replicate . | Colony location . | RNA extraction . |
|---|---|---|---|---|---|
| Bacteriome | 5 CLas (+), 5 CLas (−) | 6 months | 120, 120 | Ithaca, NY | Qiagen RNeasy |
| Head | 5 CLas (+), 5 CLas (−) | 6 months | 120, 120 | Ithaca, NY | Qiagen RNeasy |
| Salivary gland | 4 CLas (+), 4 CLas (−) | Replicates 1–3, 1 yearReplicates 4, 2 year | 150, 150 | Fort Pierce, FL | TRIzol |
| Midgut [16] | 3 CLas (+), 3 CLas (−) | <1 month | 250, 250 | Fort Pierce, FL | TRIzol |
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