Table 1.
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Temperature stability of purified stabilized HA.

HASubstitutionsTonset (°C)aTm50 (°C)
HeadHingeNeckStemRepulsive cluster
BenchmarkK209TG68YQ64YH22FE113W64.271.7 ± 0.12
Stable_HA1HA2K209TG68YQ64YH22F, S40A, S110R, H114FE113W71.874.8 ± 0.24
AWQFK209TG68YQ64YH22F, S40A, S110W, H114FE113Q69.371.5 ± 0.19
HASubstitutionsTonset (°C)aTm50 (°C)
HeadHingeNeckStemRepulsive cluster
BenchmarkK209TG68YQ64YH22FE113W64.271.7 ± 0.12
Stable_HA1HA2K209TG68YQ64YH22F, S40A, S110R, H114FE113W71.874.8 ± 0.24
AWQFK209TG68YQ64YH22F, S40A, S110W, H114FE113Q69.371.5 ± 0.19

aMean Tm50 values for benchmark HA stabilized by five substitutions in five regions of instability and two variants with additional stem substitutions (Stable_HA1HA2 and AWQF). All substitutions are in HA2 except for K209T.

Table 1.
Open in new tab

Temperature stability of purified stabilized HA.

HASubstitutionsTonset (°C)aTm50 (°C)
HeadHingeNeckStemRepulsive cluster
BenchmarkK209TG68YQ64YH22FE113W64.271.7 ± 0.12
Stable_HA1HA2K209TG68YQ64YH22F, S40A, S110R, H114FE113W71.874.8 ± 0.24
AWQFK209TG68YQ64YH22F, S40A, S110W, H114FE113Q69.371.5 ± 0.19
HASubstitutionsTonset (°C)aTm50 (°C)
HeadHingeNeckStemRepulsive cluster
BenchmarkK209TG68YQ64YH22FE113W64.271.7 ± 0.12
Stable_HA1HA2K209TG68YQ64YH22F, S40A, S110R, H114FE113W71.874.8 ± 0.24
AWQFK209TG68YQ64YH22F, S40A, S110W, H114FE113Q69.371.5 ± 0.19

aMean Tm50 values for benchmark HA stabilized by five substitutions in five regions of instability and two variants with additional stem substitutions (Stable_HA1HA2 and AWQF). All substitutions are in HA2 except for K209T.

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