Table 1. Open in new tab Ct value, copy...

Sample code	Ct value	RT-qPCR SARS-CoV-2 intrepretation result	Copy number concentration (copies/µL)	RT-ddPCR SARS-CoV-2 intrepretation result
21	35.13	35.59	36.20	Positive	1.97	0.26 (P)	0.62 (P)	Positive
22	29.16	29.69	29.67	Positive	8.2	4.1 (P)	1.24 (P)	Positive
23	29.34	28.78	29.12	Positive	0.79	10.74 (P)	7.3 (P)	Positive
24	35.65	37.06	35.74	Positive	0.61	0.09 (N)	0.09 (N)	Negative
25	35.88	34.94	35.82	Positive	N/A*	N/A*	N/A*	No amplification
26	30.44	31.35	30.72	Positive	0.88	4.27 (P)	3.54 (P)	Positive
27	33.30	34.55	34.06	Positive	1.36	0.06 (N)	0.16 (P)	Positive
28	34.56	37.19	35.05	Positive	3.81	0.08 (N)	0.19 (P)	Positive
29	35.76	35.55	35.72	Positive	1.17	0.15 (P)	0.28 (P)	Positive
30	33.01	34.23	33.47	Positive	1.08	0.59 (P)	0.21 (P)	Positive
31	37.25	36.12	38.19	Positive	0	0 (N)	0 (N)	Negative
32	29.20	29.14	29.10	Positive	0.2	5.54 (P)	6.15 (P)	Positive
33	38.42	39.09	–	Positive	3.91	0 (N)	2.01 (P)	Positive
34	22.30	22.86	22.48	Positive	240.61	220.95 (P)	125.77 (P)	Positive
35	–	–	–	Negative	0	0 (N)	0 (N)	Negative
36	30.13	30.51	30.64	Positive	3.9	3.32 (P)	0.9 (P)	Positive
37	32.53	32.59	32.69	Positive	0.27	1.25 (P)	0.69 (P)	Positive
38	32.46	33.08	32.03	Positive	2.43	1.56 (P)	4.29 (P)	Positive
39	–	–	–	Negative	0	0 (N)	0.7 (P)	Negative
40	24.43	24.67	24.62	Positive	39.44	89.96 (P)	54.57 (P)	Positive

Sample code

Ct value

RT-qPCR SARS-CoV-2 intrepretation result

Copy number concentration (copies/µL)

RT-ddPCR SARS-CoV-2 intrepretation result

ORF1ab

N**

E**

35.13

35.59

36.20

Positive

1.97

0.26 (P)

0.62 (P)

Positive

29.16

29.69

29.67

Positive

8.2

4.1 (P)

1.24 (P)

Positive

29.34

28.78

29.12

Positive

0.79

10.74 (P)

7.3 (P)

Positive

35.65

37.06

35.74

Positive

0.61

0.09 (N)

Negative

35.88

34.94

35.82

Positive

N/A*

No amplification

30.44

31.35

30.72

Positive

0.88

4.27 (P)

3.54 (P)

Positive

33.30

34.55

34.06

Positive

1.36

0.06 (N)

0.16 (P)

Positive

34.56

37.19

35.05

Positive

3.81

0.08 (N)

0.19 (P)

Positive

35.76

35.55

35.72

Positive

1.17

0.15 (P)

0.28 (P)

Positive

33.01

34.23

33.47

Positive

1.08

0.59 (P)

0.21 (P)

Positive

37.25

36.12

38.19

Positive

0 (N)

Negative

29.20

29.14

29.10

Positive

0.2

5.54 (P)

6.15 (P)

Positive

38.42

39.09

–

Positive

3.91

0 (N)

2.01 (P)

Positive

22.30

22.86

22.48

Positive

240.61

220.95 (P)

125.77 (P)

Positive

–

Negative

0 (N)

Negative

30.13

30.51

30.64

Positive

3.9

3.32 (P)

0.9 (P)

Positive

32.53

32.59

32.69

Positive

0.27

1.25 (P)

0.69 (P)

Positive

32.46

33.08

32.03

Positive

2.43

1.56 (P)

4.29 (P)

Positive

–

Negative

0 (N)

0.7 (P)

Negative

24.43

24.67

24.62

Positive

39.44

89.96 (P)

54.57 (P)

Positive

Sample code	Ct value	RT-qPCR SARS-CoV-2 intrepretation result	Copy number concentration (copies/µL)	RT-ddPCR SARS-CoV-2 intrepretation result
21	35.13	35.59	36.20	Positive	1.97	0.26 (P)	0.62 (P)	Positive
22	29.16	29.69	29.67	Positive	8.2	4.1 (P)	1.24 (P)	Positive
23	29.34	28.78	29.12	Positive	0.79	10.74 (P)	7.3 (P)	Positive
24	35.65	37.06	35.74	Positive	0.61	0.09 (N)	0.09 (N)	Negative
25	35.88	34.94	35.82	Positive	N/A*	N/A*	N/A*	No amplification
26	30.44	31.35	30.72	Positive	0.88	4.27 (P)	3.54 (P)	Positive
27	33.30	34.55	34.06	Positive	1.36	0.06 (N)	0.16 (P)	Positive
28	34.56	37.19	35.05	Positive	3.81	0.08 (N)	0.19 (P)	Positive
29	35.76	35.55	35.72	Positive	1.17	0.15 (P)	0.28 (P)	Positive
30	33.01	34.23	33.47	Positive	1.08	0.59 (P)	0.21 (P)	Positive
31	37.25	36.12	38.19	Positive	0	0 (N)	0 (N)	Negative
32	29.20	29.14	29.10	Positive	0.2	5.54 (P)	6.15 (P)	Positive
33	38.42	39.09	–	Positive	3.91	0 (N)	2.01 (P)	Positive
34	22.30	22.86	22.48	Positive	240.61	220.95 (P)	125.77 (P)	Positive
35	–	–	–	Negative	0	0 (N)	0 (N)	Negative
36	30.13	30.51	30.64	Positive	3.9	3.32 (P)	0.9 (P)	Positive
37	32.53	32.59	32.69	Positive	0.27	1.25 (P)	0.69 (P)	Positive
38	32.46	33.08	32.03	Positive	2.43	1.56 (P)	4.29 (P)	Positive
39	–	–	–	Negative	0	0 (N)	0.7 (P)	Negative
40	24.43	24.67	24.62	Positive	39.44	89.96 (P)	54.57 (P)	Positive

Table 1.

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Ct value, copy number concentration, and interpretation result of positive SARS-CoV-2 saliva specimen collection.

Sample code	Ct value			RT-qPCR SARS-CoV-2 intrepretation result	Copy number concentration (copies/µL)			RT-ddPCR SARS-CoV-2 intrepretation result
Sample code	ORF1ab	N	E	RT-qPCR SARS-CoV-2 intrepretation result	ORF1ab	N**	E**	RT-ddPCR SARS-CoV-2 intrepretation result
21	35.13	35.59	36.20	Positive	1.97	0.26 (P)	0.62 (P)	Positive
22	29.16	29.69	29.67	Positive	8.2	4.1 (P)	1.24 (P)	Positive
23	29.34	28.78	29.12	Positive	0.79	10.74 (P)	7.3 (P)	Positive
24	35.65	37.06	35.74	Positive	0.61	0.09 (N)	0.09 (N)	Negative
25	35.88	34.94	35.82	Positive	N/A*	N/A*	N/A*	No amplification
26	30.44	31.35	30.72	Positive	0.88	4.27 (P)	3.54 (P)	Positive
27	33.30	34.55	34.06	Positive	1.36	0.06 (N)	0.16 (P)	Positive
28	34.56	37.19	35.05	Positive	3.81	0.08 (N)	0.19 (P)	Positive
29	35.76	35.55	35.72	Positive	1.17	0.15 (P)	0.28 (P)	Positive
30	33.01	34.23	33.47	Positive	1.08	0.59 (P)	0.21 (P)	Positive
31	37.25	36.12	38.19	Positive	0	0 (N)	0 (N)	Negative
32	29.20	29.14	29.10	Positive	0.2	5.54 (P)	6.15 (P)	Positive
33	38.42	39.09	–	Positive	3.91	0 (N)	2.01 (P)	Positive
34	22.30	22.86	22.48	Positive	240.61	220.95 (P)	125.77 (P)	Positive
35	–	–	–	Negative	0	0 (N)	0 (N)	Negative
36	30.13	30.51	30.64	Positive	3.9	3.32 (P)	0.9 (P)	Positive
37	32.53	32.59	32.69	Positive	0.27	1.25 (P)	0.69 (P)	Positive
38	32.46	33.08	32.03	Positive	2.43	1.56 (P)	4.29 (P)	Positive
39	–	–	–	Negative	0	0 (N)	0.7 (P)	Negative
40	24.43	24.67	24.62	Positive	39.44	89.96 (P)	54.57 (P)	Positive

Sample code	Ct value			RT-qPCR SARS-CoV-2 intrepretation result	Copy number concentration (copies/µL)			RT-ddPCR SARS-CoV-2 intrepretation result
Sample code	ORF1ab	N	E	RT-qPCR SARS-CoV-2 intrepretation result	ORF1ab	N**	E**	RT-ddPCR SARS-CoV-2 intrepretation result
21	35.13	35.59	36.20	Positive	1.97	0.26 (P)	0.62 (P)	Positive
22	29.16	29.69	29.67	Positive	8.2	4.1 (P)	1.24 (P)	Positive
23	29.34	28.78	29.12	Positive	0.79	10.74 (P)	7.3 (P)	Positive
24	35.65	37.06	35.74	Positive	0.61	0.09 (N)	0.09 (N)	Negative
25	35.88	34.94	35.82	Positive	N/A*	N/A*	N/A*	No amplification
26	30.44	31.35	30.72	Positive	0.88	4.27 (P)	3.54 (P)	Positive
27	33.30	34.55	34.06	Positive	1.36	0.06 (N)	0.16 (P)	Positive
28	34.56	37.19	35.05	Positive	3.81	0.08 (N)	0.19 (P)	Positive
29	35.76	35.55	35.72	Positive	1.17	0.15 (P)	0.28 (P)	Positive
30	33.01	34.23	33.47	Positive	1.08	0.59 (P)	0.21 (P)	Positive
31	37.25	36.12	38.19	Positive	0	0 (N)	0 (N)	Negative
32	29.20	29.14	29.10	Positive	0.2	5.54 (P)	6.15 (P)	Positive
33	38.42	39.09	–	Positive	3.91	0 (N)	2.01 (P)	Positive
34	22.30	22.86	22.48	Positive	240.61	220.95 (P)	125.77 (P)	Positive
35	–	–	–	Negative	0	0 (N)	0 (N)	Negative
36	30.13	30.51	30.64	Positive	3.9	3.32 (P)	0.9 (P)	Positive
37	32.53	32.59	32.69	Positive	0.27	1.25 (P)	0.69 (P)	Positive
38	32.46	33.08	32.03	Positive	2.43	1.56 (P)	4.29 (P)	Positive
39	–	–	–	Negative	0	0 (N)	0.7 (P)	Negative
40	24.43	24.67	24.62	Positive	39.44	89.96 (P)	54.57 (P)	Positive

N/A: No amplification, droplet counts below 10 000.

Individual interpretations of positive (P) and negative (N) for the N and E genes are displayed alongside the copy number concentration values.

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