Table 1. Open in new tab Data collected...

Date	Samples collected	qRT-PCR result	Level of RNA (base on Ct value)
06/SEP/21	60	10 (17%)	1 (2%)	49 (82%)	0 (0%)	5 (8%)	55 (92%)
21/SEP/21	42	1 (2%)	1 (2%)	40 (95%)	0 (0%)	0 (0%)	42 (100%)

Date

Samples collected

qRT-PCR result

Level of RNA (base on Ct value)

Confirmed positive, n (%)

Suspected positive, n (%)

Negative, n (%)

Moderate–high (Ct < 32.0), n (%)

Low (Ct 32.0–34.9), n (%)

Very low–None (Ct > 35.0^a) n (%)

06/SEP/21

10 (17%)

1 (2%)

49 (82%)

0 (0%)

5 (8%)

55 (92%)

21/SEP/21

1 (2%)

40 (95%)

0 (0%)

42 (100%)

Date	Samples collected	qRT-PCR result	Level of RNA (base on Ct value)
06/SEP/21	60	10 (17%)	1 (2%)	49 (82%)	0 (0%)	5 (8%)	55 (92%)
21/SEP/21	42	1 (2%)	1 (2%)	40 (95%)	0 (0%)	0 (0%)	42 (100%)

Positive sample information
Open-plan office (2nd floor) 06/SEP/21	Window handle	33.6	4589.9
Meeting room (1st floor) 06/SEP/21	Air vent	37.6 (suspect^d)	25.1
Meeting room (1st floor) 06/SEP/21	Desk + chair	37.4	7.0
Canteen 06/SEP/21	Table + chair	34.2	35.2
Security office 06/SEP/21	Radio (two-way)	36.7	12.8
Security office 06/SEP/21	Telephone	34.6	583.1
Security office 06/SEP/21	Key press	34.5	148.1
Security office 06/SEP/21	Control panel	34.2	229.4
Security office 06/SEP/21	Telephone	35.1	214.2
Security office 06/SEP/21	Desk + chair	37.7	19.5
Open-plan office (ground floor) 06/SEP/21	Air conditioning unit	37.6	13.1
Canteen 21/SEP/21	Desk + chair	37.6 (suspect^d)	1.1
Canteen 21/SEP/21	Desk + chair	37.2	1.7

Positive sample information

Location
Date

Description

Mean Ct Value^b

Estimated copies per cm²^c

Open-plan office (2nd floor)
06/SEP/21

Window handle

33.6

4589.9

Meeting room (1st floor)
06/SEP/21

Air vent

37.6 (suspect^d)

25.1

Meeting room (1st floor)
06/SEP/21

Desk + chair

37.4

7.0

Canteen
06/SEP/21

Table + chair

34.2

35.2

Security office
06/SEP/21

Radio (two-way)

36.7

12.8

Security office
06/SEP/21

Telephone

34.6

583.1

Security office
06/SEP/21

Key press

34.5

148.1

Security office
06/SEP/21

Control panel

34.2

229.4

Security office
06/SEP/21

Telephone

35.1

214.2

Security office
06/SEP/21

Desk + chair

37.7

19.5

Open-plan office (ground floor) 06/SEP/21

Air conditioning unit

37.6

13.1

Canteen
21/SEP/21

Desk + chair

37.6 (suspect^d)

1.1

Canteen
21/SEP/21

Desk + chair

37.2

1.7

Positive sample information
Open-plan office (2nd floor) 06/SEP/21	Window handle	33.6	4589.9
Meeting room (1st floor) 06/SEP/21	Air vent	37.6 (suspect^d)	25.1
Meeting room (1st floor) 06/SEP/21	Desk + chair	37.4	7.0
Canteen 06/SEP/21	Table + chair	34.2	35.2
Security office 06/SEP/21	Radio (two-way)	36.7	12.8
Security office 06/SEP/21	Telephone	34.6	583.1
Security office 06/SEP/21	Key press	34.5	148.1
Security office 06/SEP/21	Control panel	34.2	229.4
Security office 06/SEP/21	Telephone	35.1	214.2
Security office 06/SEP/21	Desk + chair	37.7	19.5
Open-plan office (ground floor) 06/SEP/21	Air conditioning unit	37.6	13.1
Canteen 21/SEP/21	Desk + chair	37.6 (suspect^d)	1.1
Canteen 21/SEP/21	Desk + chair	37.2	1.7

Table 1.

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Data collected from two rounds of surface sampling in the COVID-OUT investigation of an outbreak of SARS-CoV-2 in an office-based workplace, England, August to September 2021

Date	Samples collected	qRT-PCR result			Level of RNA (base on Ct value)
Date	Samples collected	Confirmed positive, n (%)	Suspected positive, n (%)	Negative, n (%)	Moderate–high (Ct < 32.0), n (%)	Low (Ct 32.0–34.9), n (%)	Very low–None (Ct > 35.0^a) n (%)
06/SEP/21	60	10 (17%)	1 (2%)	49 (82%)	0 (0%)	5 (8%)	55 (92%)
21/SEP/21	42	1 (2%)	1 (2%)	40 (95%)	0 (0%)	0 (0%)	42 (100%)

Date	Samples collected	qRT-PCR result			Level of RNA (base on Ct value)
Date	Samples collected	Confirmed positive, n (%)	Suspected positive, n (%)	Negative, n (%)	Moderate–high (Ct < 32.0), n (%)	Low (Ct 32.0–34.9), n (%)	Very low–None (Ct > 35.0^a) n (%)
06/SEP/21	60	10 (17%)	1 (2%)	49 (82%)	0 (0%)	5 (8%)	55 (92%)
21/SEP/21	42	1 (2%)	1 (2%)	40 (95%)	0 (0%)	0 (0%)	42 (100%)

Positive sample information
Location Date	Description	Mean Ct Value^b	Estimated copies per cm²^c
Open-plan office (2nd floor) 06/SEP/21	Window handle	33.6	4589.9
Meeting room (1st floor) 06/SEP/21	Air vent	37.6 (suspect^d)	25.1
Meeting room (1st floor) 06/SEP/21	Desk + chair	37.4	7.0
Canteen 06/SEP/21	Table + chair	34.2	35.2
Security office 06/SEP/21	Radio (two-way)	36.7	12.8
Security office 06/SEP/21	Telephone	34.6	583.1
Security office 06/SEP/21	Key press	34.5	148.1
Security office 06/SEP/21	Control panel	34.2	229.4
Security office 06/SEP/21	Telephone	35.1	214.2
Security office 06/SEP/21	Desk + chair	37.7	19.5
Open-plan office (ground floor) 06/SEP/21	Air conditioning unit	37.6	13.1
Canteen 21/SEP/21	Desk + chair	37.6 (suspect^d)	1.1
Canteen 21/SEP/21	Desk + chair	37.2	1.7

Positive sample information
Location Date	Description	Mean Ct Value^b	Estimated copies per cm²^c
Open-plan office (2nd floor) 06/SEP/21	Window handle	33.6	4589.9
Meeting room (1st floor) 06/SEP/21	Air vent	37.6 (suspect^d)	25.1
Meeting room (1st floor) 06/SEP/21	Desk + chair	37.4	7.0
Canteen 06/SEP/21	Table + chair	34.2	35.2
Security office 06/SEP/21	Radio (two-way)	36.7	12.8
Security office 06/SEP/21	Telephone	34.6	583.1
Security office 06/SEP/21	Key press	34.5	148.1
Security office 06/SEP/21	Control panel	34.2	229.4
Security office 06/SEP/21	Telephone	35.1	214.2
Security office 06/SEP/21	Desk + chair	37.7	19.5
Open-plan office (ground floor) 06/SEP/21	Air conditioning unit	37.6	13.1
Canteen 21/SEP/21	Desk + chair	37.6 (suspect^d)	1.1
Canteen 21/SEP/21	Desk + chair	37.2	1.7

RNA, ribonucleic acid.

^aIncludes samples with no SARS-CoV-2 RNA detected.

^bMean Ct value for the N gene.

^cExtrapolation from copies per reaction to copies per sample collected based on the dilution factor, then divided by recorded sampling area.

^dA ‘suspected positive’ is one where only a single replicate is positive in at least one target.

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