Table 2

Comparison between settings applied in databases to call m|$^{6}$|A

DatabasesFilteringPeak-callingReported-peaksCustomizable setting of parameters for peak calling?Reported m|$^{6}$|A sites
MeT-DB/
MeT-DBv2.0
exomePeakFDR<0.05, P <0.05,
FC>1 and MAPQ>30
unavailableSearching the RRACH motif
in the identified peaks
RMBase/
RMBasev2.0
exomePeakFDR<0.05, P <0.01,
MAPQ>30 and FC>2
not allowedSearching the RRACH motif
in the identified peaks
CVm6APicard (remove
PCR duplicates)
MeTPeakFDR<0.05, FE>1
and MAPQ>30
not allowedmiCLIP-seq/PA-m|$^{6}$|A-seq
REPICRemoved rRNAs,
FastQ Screen
exomePeak, MeTPeak,
MACS2
FDR<0.05, MAPQ>20,
FE>2
not allowedm|$^{6}$|A-seq and meRIP-seq
m6ASNP/
m6AVar/RMVar
MACS2, MeTPeak,
Meyer’s method
Consensus peak by MSPCnot allowedmiCLIP/MAZTER-seq/m|$^{6}$|A-REF-seq/m|$^{6}$|ACE-seq/
DART-seq/PA-m|$^{6}$|A-Seq/m|$^{6}$|A-Label-seq (High confidence);
meRIP-Seq (Medium confidence);
Transcriptome-wide
prediction (Low confidence)
m6A-Atlas/
m6A-Atlas v2.0
exomePeak2, MACS2
(v2.0), TRESS (v2.0)
Fold-enrichment>1not allowedm|$^{6}$|A-REF-seq/MAZTER-seq/miCLIP-seq/
m|$^{6}$|A-CLIP-seq/PA-m|$^{6}$|A-seq/m|$^{6}$|A-seq
with improved protocol/m|$^{6}$|A-CLIP-seq
m6A-TSHubexomePeak2P <1e-10, contain
at least one DRACH
not allowedmiCLIP/m|$^{6}$|A-CLIP-seq, m|$^{6}$|A-REF-seq
and DART-seq
DatabasesFilteringPeak-callingReported-peaksCustomizable setting of parameters for peak calling?Reported m|$^{6}$|A sites
MeT-DB/
MeT-DBv2.0
exomePeakFDR<0.05, P <0.05,
FC>1 and MAPQ>30
unavailableSearching the RRACH motif
in the identified peaks
RMBase/
RMBasev2.0
exomePeakFDR<0.05, P <0.01,
MAPQ>30 and FC>2
not allowedSearching the RRACH motif
in the identified peaks
CVm6APicard (remove
PCR duplicates)
MeTPeakFDR<0.05, FE>1
and MAPQ>30
not allowedmiCLIP-seq/PA-m|$^{6}$|A-seq
REPICRemoved rRNAs,
FastQ Screen
exomePeak, MeTPeak,
MACS2
FDR<0.05, MAPQ>20,
FE>2
not allowedm|$^{6}$|A-seq and meRIP-seq
m6ASNP/
m6AVar/RMVar
MACS2, MeTPeak,
Meyer’s method
Consensus peak by MSPCnot allowedmiCLIP/MAZTER-seq/m|$^{6}$|A-REF-seq/m|$^{6}$|ACE-seq/
DART-seq/PA-m|$^{6}$|A-Seq/m|$^{6}$|A-Label-seq (High confidence);
meRIP-Seq (Medium confidence);
Transcriptome-wide
prediction (Low confidence)
m6A-Atlas/
m6A-Atlas v2.0
exomePeak2, MACS2
(v2.0), TRESS (v2.0)
Fold-enrichment>1not allowedm|$^{6}$|A-REF-seq/MAZTER-seq/miCLIP-seq/
m|$^{6}$|A-CLIP-seq/PA-m|$^{6}$|A-seq/m|$^{6}$|A-seq
with improved protocol/m|$^{6}$|A-CLIP-seq
m6A-TSHubexomePeak2P <1e-10, contain
at least one DRACH
not allowedmiCLIP/m|$^{6}$|A-CLIP-seq, m|$^{6}$|A-REF-seq
and DART-seq
Table 2

Comparison between settings applied in databases to call m|$^{6}$|A

DatabasesFilteringPeak-callingReported-peaksCustomizable setting of parameters for peak calling?Reported m|$^{6}$|A sites
MeT-DB/
MeT-DBv2.0
exomePeakFDR<0.05, P <0.05,
FC>1 and MAPQ>30
unavailableSearching the RRACH motif
in the identified peaks
RMBase/
RMBasev2.0
exomePeakFDR<0.05, P <0.01,
MAPQ>30 and FC>2
not allowedSearching the RRACH motif
in the identified peaks
CVm6APicard (remove
PCR duplicates)
MeTPeakFDR<0.05, FE>1
and MAPQ>30
not allowedmiCLIP-seq/PA-m|$^{6}$|A-seq
REPICRemoved rRNAs,
FastQ Screen
exomePeak, MeTPeak,
MACS2
FDR<0.05, MAPQ>20,
FE>2
not allowedm|$^{6}$|A-seq and meRIP-seq
m6ASNP/
m6AVar/RMVar
MACS2, MeTPeak,
Meyer’s method
Consensus peak by MSPCnot allowedmiCLIP/MAZTER-seq/m|$^{6}$|A-REF-seq/m|$^{6}$|ACE-seq/
DART-seq/PA-m|$^{6}$|A-Seq/m|$^{6}$|A-Label-seq (High confidence);
meRIP-Seq (Medium confidence);
Transcriptome-wide
prediction (Low confidence)
m6A-Atlas/
m6A-Atlas v2.0
exomePeak2, MACS2
(v2.0), TRESS (v2.0)
Fold-enrichment>1not allowedm|$^{6}$|A-REF-seq/MAZTER-seq/miCLIP-seq/
m|$^{6}$|A-CLIP-seq/PA-m|$^{6}$|A-seq/m|$^{6}$|A-seq
with improved protocol/m|$^{6}$|A-CLIP-seq
m6A-TSHubexomePeak2P <1e-10, contain
at least one DRACH
not allowedmiCLIP/m|$^{6}$|A-CLIP-seq, m|$^{6}$|A-REF-seq
and DART-seq
DatabasesFilteringPeak-callingReported-peaksCustomizable setting of parameters for peak calling?Reported m|$^{6}$|A sites
MeT-DB/
MeT-DBv2.0
exomePeakFDR<0.05, P <0.05,
FC>1 and MAPQ>30
unavailableSearching the RRACH motif
in the identified peaks
RMBase/
RMBasev2.0
exomePeakFDR<0.05, P <0.01,
MAPQ>30 and FC>2
not allowedSearching the RRACH motif
in the identified peaks
CVm6APicard (remove
PCR duplicates)
MeTPeakFDR<0.05, FE>1
and MAPQ>30
not allowedmiCLIP-seq/PA-m|$^{6}$|A-seq
REPICRemoved rRNAs,
FastQ Screen
exomePeak, MeTPeak,
MACS2
FDR<0.05, MAPQ>20,
FE>2
not allowedm|$^{6}$|A-seq and meRIP-seq
m6ASNP/
m6AVar/RMVar
MACS2, MeTPeak,
Meyer’s method
Consensus peak by MSPCnot allowedmiCLIP/MAZTER-seq/m|$^{6}$|A-REF-seq/m|$^{6}$|ACE-seq/
DART-seq/PA-m|$^{6}$|A-Seq/m|$^{6}$|A-Label-seq (High confidence);
meRIP-Seq (Medium confidence);
Transcriptome-wide
prediction (Low confidence)
m6A-Atlas/
m6A-Atlas v2.0
exomePeak2, MACS2
(v2.0), TRESS (v2.0)
Fold-enrichment>1not allowedm|$^{6}$|A-REF-seq/MAZTER-seq/miCLIP-seq/
m|$^{6}$|A-CLIP-seq/PA-m|$^{6}$|A-seq/m|$^{6}$|A-seq
with improved protocol/m|$^{6}$|A-CLIP-seq
m6A-TSHubexomePeak2P <1e-10, contain
at least one DRACH
not allowedmiCLIP/m|$^{6}$|A-CLIP-seq, m|$^{6}$|A-REF-seq
and DART-seq
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