Table 1.
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Primers and Probes Used for Quantitative Polymerase Chain Reaction to Detect Typhoidal Salmonella

OligoSequence (5′ to 3′) and FluorophoreTargetReference
oriC-probeFAM-TGATCTTCAGTGTTTCCCCAACCTGTTTTG-QSYSalmonella enterica (all serovars)[21]
oriC-FAGCCAAATCTCCGCTGGATSalmonella enterica (all serovars)[21]
oriC-RCGGAACTGAAAGGCGCTGSalmonella enterica (all serovars)[21]
ST-probeVIC-CATTTGTTCTGGAGCAGGCTGACGG-QSYSalmonella Typhi staG (STY0201)[19]
ST-FrtCGCGAAGTCAGAGTCGACATAGSalmonella Typhi staG (STY0201)[19]
ST-RrtAAGACCTCAACGCCGATCACSalmonella Typhi staG (STY0201)[19]
PhHV-probeABY-TTTTTATGTGTCCGCCACCATCTGGATC-QSYRecombinant pCR TOPO 2.1 gB[19]
PhHV-FrtGGGCGAATCACAGATTGAATCRecombinant pCR TOPO 2.1 gB[19]
PhHV-RrtGCGGTTCCAAACGTACCAARecombinant pCR TOPO 2.1 gB[19]
OligoSequence (5′ to 3′) and FluorophoreTargetReference
oriC-probeFAM-TGATCTTCAGTGTTTCCCCAACCTGTTTTG-QSYSalmonella enterica (all serovars)[21]
oriC-FAGCCAAATCTCCGCTGGATSalmonella enterica (all serovars)[21]
oriC-RCGGAACTGAAAGGCGCTGSalmonella enterica (all serovars)[21]
ST-probeVIC-CATTTGTTCTGGAGCAGGCTGACGG-QSYSalmonella Typhi staG (STY0201)[19]
ST-FrtCGCGAAGTCAGAGTCGACATAGSalmonella Typhi staG (STY0201)[19]
ST-RrtAAGACCTCAACGCCGATCACSalmonella Typhi staG (STY0201)[19]
PhHV-probeABY-TTTTTATGTGTCCGCCACCATCTGGATC-QSYRecombinant pCR TOPO 2.1 gB[19]
PhHV-FrtGGGCGAATCACAGATTGAATCRecombinant pCR TOPO 2.1 gB[19]
PhHV-RrtGCGGTTCCAAACGTACCAARecombinant pCR TOPO 2.1 gB[19]

Abbreviations: PhHV, phocine herpesvirus.

Table 1.
Open in new tab

Primers and Probes Used for Quantitative Polymerase Chain Reaction to Detect Typhoidal Salmonella

OligoSequence (5′ to 3′) and FluorophoreTargetReference
oriC-probeFAM-TGATCTTCAGTGTTTCCCCAACCTGTTTTG-QSYSalmonella enterica (all serovars)[21]
oriC-FAGCCAAATCTCCGCTGGATSalmonella enterica (all serovars)[21]
oriC-RCGGAACTGAAAGGCGCTGSalmonella enterica (all serovars)[21]
ST-probeVIC-CATTTGTTCTGGAGCAGGCTGACGG-QSYSalmonella Typhi staG (STY0201)[19]
ST-FrtCGCGAAGTCAGAGTCGACATAGSalmonella Typhi staG (STY0201)[19]
ST-RrtAAGACCTCAACGCCGATCACSalmonella Typhi staG (STY0201)[19]
PhHV-probeABY-TTTTTATGTGTCCGCCACCATCTGGATC-QSYRecombinant pCR TOPO 2.1 gB[19]
PhHV-FrtGGGCGAATCACAGATTGAATCRecombinant pCR TOPO 2.1 gB[19]
PhHV-RrtGCGGTTCCAAACGTACCAARecombinant pCR TOPO 2.1 gB[19]
OligoSequence (5′ to 3′) and FluorophoreTargetReference
oriC-probeFAM-TGATCTTCAGTGTTTCCCCAACCTGTTTTG-QSYSalmonella enterica (all serovars)[21]
oriC-FAGCCAAATCTCCGCTGGATSalmonella enterica (all serovars)[21]
oriC-RCGGAACTGAAAGGCGCTGSalmonella enterica (all serovars)[21]
ST-probeVIC-CATTTGTTCTGGAGCAGGCTGACGG-QSYSalmonella Typhi staG (STY0201)[19]
ST-FrtCGCGAAGTCAGAGTCGACATAGSalmonella Typhi staG (STY0201)[19]
ST-RrtAAGACCTCAACGCCGATCACSalmonella Typhi staG (STY0201)[19]
PhHV-probeABY-TTTTTATGTGTCCGCCACCATCTGGATC-QSYRecombinant pCR TOPO 2.1 gB[19]
PhHV-FrtGGGCGAATCACAGATTGAATCRecombinant pCR TOPO 2.1 gB[19]
PhHV-RrtGCGGTTCCAAACGTACCAARecombinant pCR TOPO 2.1 gB[19]

Abbreviations: PhHV, phocine herpesvirus.

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