List of Ala-substituted peptide sequences compared to Myb397–415 and relative peptide-induced thermal stabilization (ΔT1/2) of c-KIT2 G4, assessed by CD melting experiments performed using 2 mol equiv of each peptide with respect to G4
| Name . | Sequence (Nterm → Cterm) . | ΔT1/2 (°C)a . |
|---|---|---|
| Myb397–415 | NHTGNSIRHRFRVYLSKRL | 17.3 (±0.2) |
| MybA397 | AHTGNSIRHRFRVYLSKRL | 18.0 (±0.2) |
| MybA398 | NATGNSIRHRFRVYLSKRL | 19.8 (±0.2) |
| MybA399 | NHAGNSIRHRFRVYLSKRL | 18.5 (±0.2) |
| MybA400 | NHTANSIRHRFRVYLSKRL | 21.1 (±0.4) |
| MybA401 | NHTGASIRHRFRVYLSKRL | 19.7 (±0.2) |
| MybA402 | NHTGNAIRHRFRVYLSKRL | 18.5 (±0.2) |
| MybA403 | NHTGNSARHRFRVYLSKRL | 17.7 (±0.2) |
| MybA404 | NHTGNSIAHRFRVYLSKRL | 16.6 (±0.2) |
| MybA405 | NHTGNSIRARFRVYLSKRL | 16.0 (±0.2) |
| MybA406 | NHTGNSIRHAFRVYLSKRL | 12.1 (±0.3) |
| MybA407 | NHTGNSIRHRARVYLSKRL | 17.8 (±0.2) |
| MybA408 | NHTGNSIRHRFAVYLSKRL | 14.0 (±0.6) |
| MybA409 | NHTGNSIRHRFRAYLSKRL | 14.7 (±0.6) |
| MybA410 | NHTGNSIRHRFRVALSKRL | 13.6 (±0.2) |
| MybA411 | NHTGNSIRHRFRVYASKRL | 19.5 (±0.4) |
| MybA412 | NHTGNSIRHRFRVYLAKRL | 23.3 (±0.2) |
| MybA413 | NHTGNSIRHRFRVYLSARL | 10.7 (±0.6) |
| MybA414 | NHTGNSIRHRFRVYLSKAL | 10.7 (±0.6) |
| MybA415 | NHTGNSIRHRFRVYLSKRA | 17.8 (±0.5) |
| Name . | Sequence (Nterm → Cterm) . | ΔT1/2 (°C)a . |
|---|---|---|
| Myb397–415 | NHTGNSIRHRFRVYLSKRL | 17.3 (±0.2) |
| MybA397 | AHTGNSIRHRFRVYLSKRL | 18.0 (±0.2) |
| MybA398 | NATGNSIRHRFRVYLSKRL | 19.8 (±0.2) |
| MybA399 | NHAGNSIRHRFRVYLSKRL | 18.5 (±0.2) |
| MybA400 | NHTANSIRHRFRVYLSKRL | 21.1 (±0.4) |
| MybA401 | NHTGASIRHRFRVYLSKRL | 19.7 (±0.2) |
| MybA402 | NHTGNAIRHRFRVYLSKRL | 18.5 (±0.2) |
| MybA403 | NHTGNSARHRFRVYLSKRL | 17.7 (±0.2) |
| MybA404 | NHTGNSIAHRFRVYLSKRL | 16.6 (±0.2) |
| MybA405 | NHTGNSIRARFRVYLSKRL | 16.0 (±0.2) |
| MybA406 | NHTGNSIRHAFRVYLSKRL | 12.1 (±0.3) |
| MybA407 | NHTGNSIRHRARVYLSKRL | 17.8 (±0.2) |
| MybA408 | NHTGNSIRHRFAVYLSKRL | 14.0 (±0.6) |
| MybA409 | NHTGNSIRHRFRAYLSKRL | 14.7 (±0.6) |
| MybA410 | NHTGNSIRHRFRVALSKRL | 13.6 (±0.2) |
| MybA411 | NHTGNSIRHRFRVYASKRL | 19.5 (±0.4) |
| MybA412 | NHTGNSIRHRFRVYLAKRL | 23.3 (±0.2) |
| MybA413 | NHTGNSIRHRFRVYLSARL | 10.7 (±0.6) |
| MybA414 | NHTGNSIRHRFRVYLSKAL | 10.7 (±0.6) |
| MybA415 | NHTGNSIRHRFRVYLSKRA | 17.8 (±0.5) |
aΔT1/2 represents the difference in melting temperature [ΔT1/2= T1/2 (DNA + peptide) – T1/2 (DNA)]. T1/2 of c-KIT2 alone = 59.5 (±0.1)°C.
List of Ala-substituted peptide sequences compared to Myb397–415 and relative peptide-induced thermal stabilization (ΔT1/2) of c-KIT2 G4, assessed by CD melting experiments performed using 2 mol equiv of each peptide with respect to G4
| Name . | Sequence (Nterm → Cterm) . | ΔT1/2 (°C)a . |
|---|---|---|
| Myb397–415 | NHTGNSIRHRFRVYLSKRL | 17.3 (±0.2) |
| MybA397 | AHTGNSIRHRFRVYLSKRL | 18.0 (±0.2) |
| MybA398 | NATGNSIRHRFRVYLSKRL | 19.8 (±0.2) |
| MybA399 | NHAGNSIRHRFRVYLSKRL | 18.5 (±0.2) |
| MybA400 | NHTANSIRHRFRVYLSKRL | 21.1 (±0.4) |
| MybA401 | NHTGASIRHRFRVYLSKRL | 19.7 (±0.2) |
| MybA402 | NHTGNAIRHRFRVYLSKRL | 18.5 (±0.2) |
| MybA403 | NHTGNSARHRFRVYLSKRL | 17.7 (±0.2) |
| MybA404 | NHTGNSIAHRFRVYLSKRL | 16.6 (±0.2) |
| MybA405 | NHTGNSIRARFRVYLSKRL | 16.0 (±0.2) |
| MybA406 | NHTGNSIRHAFRVYLSKRL | 12.1 (±0.3) |
| MybA407 | NHTGNSIRHRARVYLSKRL | 17.8 (±0.2) |
| MybA408 | NHTGNSIRHRFAVYLSKRL | 14.0 (±0.6) |
| MybA409 | NHTGNSIRHRFRAYLSKRL | 14.7 (±0.6) |
| MybA410 | NHTGNSIRHRFRVALSKRL | 13.6 (±0.2) |
| MybA411 | NHTGNSIRHRFRVYASKRL | 19.5 (±0.4) |
| MybA412 | NHTGNSIRHRFRVYLAKRL | 23.3 (±0.2) |
| MybA413 | NHTGNSIRHRFRVYLSARL | 10.7 (±0.6) |
| MybA414 | NHTGNSIRHRFRVYLSKAL | 10.7 (±0.6) |
| MybA415 | NHTGNSIRHRFRVYLSKRA | 17.8 (±0.5) |
| Name . | Sequence (Nterm → Cterm) . | ΔT1/2 (°C)a . |
|---|---|---|
| Myb397–415 | NHTGNSIRHRFRVYLSKRL | 17.3 (±0.2) |
| MybA397 | AHTGNSIRHRFRVYLSKRL | 18.0 (±0.2) |
| MybA398 | NATGNSIRHRFRVYLSKRL | 19.8 (±0.2) |
| MybA399 | NHAGNSIRHRFRVYLSKRL | 18.5 (±0.2) |
| MybA400 | NHTANSIRHRFRVYLSKRL | 21.1 (±0.4) |
| MybA401 | NHTGASIRHRFRVYLSKRL | 19.7 (±0.2) |
| MybA402 | NHTGNAIRHRFRVYLSKRL | 18.5 (±0.2) |
| MybA403 | NHTGNSARHRFRVYLSKRL | 17.7 (±0.2) |
| MybA404 | NHTGNSIAHRFRVYLSKRL | 16.6 (±0.2) |
| MybA405 | NHTGNSIRARFRVYLSKRL | 16.0 (±0.2) |
| MybA406 | NHTGNSIRHAFRVYLSKRL | 12.1 (±0.3) |
| MybA407 | NHTGNSIRHRARVYLSKRL | 17.8 (±0.2) |
| MybA408 | NHTGNSIRHRFAVYLSKRL | 14.0 (±0.6) |
| MybA409 | NHTGNSIRHRFRAYLSKRL | 14.7 (±0.6) |
| MybA410 | NHTGNSIRHRFRVALSKRL | 13.6 (±0.2) |
| MybA411 | NHTGNSIRHRFRVYASKRL | 19.5 (±0.4) |
| MybA412 | NHTGNSIRHRFRVYLAKRL | 23.3 (±0.2) |
| MybA413 | NHTGNSIRHRFRVYLSARL | 10.7 (±0.6) |
| MybA414 | NHTGNSIRHRFRVYLSKAL | 10.7 (±0.6) |
| MybA415 | NHTGNSIRHRFRVYLSKRA | 17.8 (±0.5) |
aΔT1/2 represents the difference in melting temperature [ΔT1/2= T1/2 (DNA + peptide) – T1/2 (DNA)]. T1/2 of c-KIT2 alone = 59.5 (±0.1)°C.
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