| Method . | Generation . | Read length (bp) . | Single pass error rate (%) . | No. of reads per run . | Time per run . | Cost per million bases (USD) . | Refs. . |
|---|---|---|---|---|---|---|---|
| Sanger ABI 3730×l | 1st | 600–1000 | 0.001 | 96 | 0.5–3 h | 500 | [14,18–21] |
| Ion Torrent | 2nd | 200 | 1 | 8.2 × 107 | 2–4 h | 0.1 | [15,25] |
| 454 (Roche) GS FLX+ | 2nd | 700 | 1 | 1 × 106 | 23 h | 8.57 | [14,17,27] |
| Illumina HiSeq 2500 (High Output) | 2nd | 2 × 125 | 0.1 | 8 × 109 (paired) | 7–60 h | 0.03 | [9,16,26] |
| Illumina HiSeq 2500 (Rapid Run) | 2nd | 2 × 250 | 0.1 | 1.2 × 109 (paired) | 1–6 days | 0.04 | [9,16,26] |
| SOLiD 5500×l | 2nd | 2 × 60 | 5 | 8 × 108 | 6 days | 0.11 | [14,24] |
| PacBio RS II: P6-C4 | 3rd | 1.0–1.5 × 104 on average | 13 | 3.5–7.5 × 104 | 0.5–4 h | 0.40–0.80 | [5,12,15] |
| Oxford Nanopore MinION | 3rd | 2–5 × 103 on average | 38 | 1.1–4.7 × 104 | 50 h | 6.44–17.90 | [22,23] |
| Method . | Generation . | Read length (bp) . | Single pass error rate (%) . | No. of reads per run . | Time per run . | Cost per million bases (USD) . | Refs. . |
|---|---|---|---|---|---|---|---|
| Sanger ABI 3730×l | 1st | 600–1000 | 0.001 | 96 | 0.5–3 h | 500 | [14,18–21] |
| Ion Torrent | 2nd | 200 | 1 | 8.2 × 107 | 2–4 h | 0.1 | [15,25] |
| 454 (Roche) GS FLX+ | 2nd | 700 | 1 | 1 × 106 | 23 h | 8.57 | [14,17,27] |
| Illumina HiSeq 2500 (High Output) | 2nd | 2 × 125 | 0.1 | 8 × 109 (paired) | 7–60 h | 0.03 | [9,16,26] |
| Illumina HiSeq 2500 (Rapid Run) | 2nd | 2 × 250 | 0.1 | 1.2 × 109 (paired) | 1–6 days | 0.04 | [9,16,26] |
| SOLiD 5500×l | 2nd | 2 × 60 | 5 | 8 × 108 | 6 days | 0.11 | [14,24] |
| PacBio RS II: P6-C4 | 3rd | 1.0–1.5 × 104 on average | 13 | 3.5–7.5 × 104 | 0.5–4 h | 0.40–0.80 | [5,12,15] |
| Oxford Nanopore MinION | 3rd | 2–5 × 103 on average | 38 | 1.1–4.7 × 104 | 50 h | 6.44–17.90 | [22,23] |
| Method . | Generation . | Read length (bp) . | Single pass error rate (%) . | No. of reads per run . | Time per run . | Cost per million bases (USD) . | Refs. . |
|---|---|---|---|---|---|---|---|
| Sanger ABI 3730×l | 1st | 600–1000 | 0.001 | 96 | 0.5–3 h | 500 | [14,18–21] |
| Ion Torrent | 2nd | 200 | 1 | 8.2 × 107 | 2–4 h | 0.1 | [15,25] |
| 454 (Roche) GS FLX+ | 2nd | 700 | 1 | 1 × 106 | 23 h | 8.57 | [14,17,27] |
| Illumina HiSeq 2500 (High Output) | 2nd | 2 × 125 | 0.1 | 8 × 109 (paired) | 7–60 h | 0.03 | [9,16,26] |
| Illumina HiSeq 2500 (Rapid Run) | 2nd | 2 × 250 | 0.1 | 1.2 × 109 (paired) | 1–6 days | 0.04 | [9,16,26] |
| SOLiD 5500×l | 2nd | 2 × 60 | 5 | 8 × 108 | 6 days | 0.11 | [14,24] |
| PacBio RS II: P6-C4 | 3rd | 1.0–1.5 × 104 on average | 13 | 3.5–7.5 × 104 | 0.5–4 h | 0.40–0.80 | [5,12,15] |
| Oxford Nanopore MinION | 3rd | 2–5 × 103 on average | 38 | 1.1–4.7 × 104 | 50 h | 6.44–17.90 | [22,23] |
| Method . | Generation . | Read length (bp) . | Single pass error rate (%) . | No. of reads per run . | Time per run . | Cost per million bases (USD) . | Refs. . |
|---|---|---|---|---|---|---|---|
| Sanger ABI 3730×l | 1st | 600–1000 | 0.001 | 96 | 0.5–3 h | 500 | [14,18–21] |
| Ion Torrent | 2nd | 200 | 1 | 8.2 × 107 | 2–4 h | 0.1 | [15,25] |
| 454 (Roche) GS FLX+ | 2nd | 700 | 1 | 1 × 106 | 23 h | 8.57 | [14,17,27] |
| Illumina HiSeq 2500 (High Output) | 2nd | 2 × 125 | 0.1 | 8 × 109 (paired) | 7–60 h | 0.03 | [9,16,26] |
| Illumina HiSeq 2500 (Rapid Run) | 2nd | 2 × 250 | 0.1 | 1.2 × 109 (paired) | 1–6 days | 0.04 | [9,16,26] |
| SOLiD 5500×l | 2nd | 2 × 60 | 5 | 8 × 108 | 6 days | 0.11 | [14,24] |
| PacBio RS II: P6-C4 | 3rd | 1.0–1.5 × 104 on average | 13 | 3.5–7.5 × 104 | 0.5–4 h | 0.40–0.80 | [5,12,15] |
| Oxford Nanopore MinION | 3rd | 2–5 × 103 on average | 38 | 1.1–4.7 × 104 | 50 h | 6.44–17.90 | [22,23] |
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