Sequence lengths per treatment combination. Treatment combinations are sorted from best to worst based on the highest “Storage” values, followed by “Sequence lengths” values. Column “Storage” represents the last time we were able to obtain sequences from the specimen in a condition that enables species determination (censoring time 24 months). We were looking for the highest “Sequence lengths” with any value above 500 considered acceptable. The remaining columns represent species identification success rates
| Fixative fluid . | Storage fluid . | Storage (months) . | Sequence length . | GenBank identity score . | % similarity . | E-value . | ID correct . |
|---|---|---|---|---|---|---|---|
| Acetic alcohol | Acetone | 24 | 794 | 785/794 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Acetone | 24 | 789 | 785/789 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | 80% ethanol | 24 | 788 | 787/788 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Absolute ethanol | 24 | 788 | 783/788 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | Acetone | 24 | 788 | 786/788 | 99 | 0.0 | Yes |
| KAA | Acetone | 24 | 787 | 785/787 | 99 | 0.0 | Yes |
| KAA | 70% ethanol | 24 | 782 | 779/783 | 99 | 0.0 | Yes |
| Absolute ethanol cold | 70% ethanol | 24 | 781 | 779/781 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | 70% ethanol | 24 | 780 | 776/780 | 99 | 0.0 | Yes |
| KAA | 80% ethanol | 24 | 780 | 773/780 | 99 | 0.0 | Yes |
| Acetic alcohol | 80% ethanol | 24 | 774 | 768/774 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | Absolute ethanol | 24 | 772 | 767/772 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | Absolute ethanol | 24 | 763 | 760/763 | 99 | 0.0 | Yes |
| Acetic alcohol | Absolute ethanol | 24 | 675 | 669/675 | 99 | 0.0 | Yes |
| Absolute ethanol cold | 80% ethanol | 24 | 674 | 672/674 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | 70% ethanol | 24 | 604 | 599/604 | 99 | 0.0 | Yes |
| KAA | Absolute ethanol | 24 | 604 | 595/604 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | 80% ethanol | 24 | 575 | 571/575 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | 80% ethanol | 24 | 565 | 562/565 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | Acetone | 24 | 553 | 549/553 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | 70% ethanol | 24 | 430 | 426/430 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Absolute ethanol | 24 | 385 | 380/385 | 99 | 0.0 | Yes |
| KAA | Acetic alcohol | 22 | 792 | 785/789 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Acetic alcohol | 22 | 791 | 791/791 | 100 | 0.0 | Yes |
| HWK 80 °C 5 min | AGA | 22 | 391 | 390/391 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Acetone | 22 | 317 | 315/317 | 99 | 5E-159 | Yes |
| Acetic alcohol | 70% ethanol | 22 | 287 | 283/287 | 99 | 4E-139 | Yes |
| Absolute ethanol boiling | AGA | 22 | 125 | 124/125 | 99 | 5E-55 | Yes |
| Acetic alcohol | Acetic alcohol | 20 | 598 | 596/598 | 99 | 0.0 | Yes |
| Acetic alcohol | AGA | 8 | 803 | 800/803 | 99 | s | Yes |
| Absolute ethanol cold | AGA | 8 | 778 | 776/778 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Acetic alcohol | 8 | 754 | 753/754 | 99 | 0.0 | Yes |
| KAA | AGA | 8 | 216 | 209/216 | 99 | 3E-95 | Yes |
| HWK 80 °C 6 h | AGA | 8 | 171 | 167/169 | 99 | 1E-77 | Yes |
| Absolute ethanol boiling | Acetic alcohol | 8 | 105 | 105/105 | 100 | 1E-45 | Yes |
| HWK 80 °C 6 h | Acetic alcohol | 6 | 808 | 803/808 | 99 | 0.0 | Yes |
| Fixative fluid . | Storage fluid . | Storage (months) . | Sequence length . | GenBank identity score . | % similarity . | E-value . | ID correct . |
|---|---|---|---|---|---|---|---|
| Acetic alcohol | Acetone | 24 | 794 | 785/794 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Acetone | 24 | 789 | 785/789 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | 80% ethanol | 24 | 788 | 787/788 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Absolute ethanol | 24 | 788 | 783/788 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | Acetone | 24 | 788 | 786/788 | 99 | 0.0 | Yes |
| KAA | Acetone | 24 | 787 | 785/787 | 99 | 0.0 | Yes |
| KAA | 70% ethanol | 24 | 782 | 779/783 | 99 | 0.0 | Yes |
| Absolute ethanol cold | 70% ethanol | 24 | 781 | 779/781 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | 70% ethanol | 24 | 780 | 776/780 | 99 | 0.0 | Yes |
| KAA | 80% ethanol | 24 | 780 | 773/780 | 99 | 0.0 | Yes |
| Acetic alcohol | 80% ethanol | 24 | 774 | 768/774 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | Absolute ethanol | 24 | 772 | 767/772 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | Absolute ethanol | 24 | 763 | 760/763 | 99 | 0.0 | Yes |
| Acetic alcohol | Absolute ethanol | 24 | 675 | 669/675 | 99 | 0.0 | Yes |
| Absolute ethanol cold | 80% ethanol | 24 | 674 | 672/674 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | 70% ethanol | 24 | 604 | 599/604 | 99 | 0.0 | Yes |
| KAA | Absolute ethanol | 24 | 604 | 595/604 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | 80% ethanol | 24 | 575 | 571/575 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | 80% ethanol | 24 | 565 | 562/565 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | Acetone | 24 | 553 | 549/553 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | 70% ethanol | 24 | 430 | 426/430 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Absolute ethanol | 24 | 385 | 380/385 | 99 | 0.0 | Yes |
| KAA | Acetic alcohol | 22 | 792 | 785/789 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Acetic alcohol | 22 | 791 | 791/791 | 100 | 0.0 | Yes |
| HWK 80 °C 5 min | AGA | 22 | 391 | 390/391 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Acetone | 22 | 317 | 315/317 | 99 | 5E-159 | Yes |
| Acetic alcohol | 70% ethanol | 22 | 287 | 283/287 | 99 | 4E-139 | Yes |
| Absolute ethanol boiling | AGA | 22 | 125 | 124/125 | 99 | 5E-55 | Yes |
| Acetic alcohol | Acetic alcohol | 20 | 598 | 596/598 | 99 | 0.0 | Yes |
| Acetic alcohol | AGA | 8 | 803 | 800/803 | 99 | s | Yes |
| Absolute ethanol cold | AGA | 8 | 778 | 776/778 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Acetic alcohol | 8 | 754 | 753/754 | 99 | 0.0 | Yes |
| KAA | AGA | 8 | 216 | 209/216 | 99 | 3E-95 | Yes |
| HWK 80 °C 6 h | AGA | 8 | 171 | 167/169 | 99 | 1E-77 | Yes |
| Absolute ethanol boiling | Acetic alcohol | 8 | 105 | 105/105 | 100 | 1E-45 | Yes |
| HWK 80 °C 6 h | Acetic alcohol | 6 | 808 | 803/808 | 99 | 0.0 | Yes |
Sequence lengths per treatment combination. Treatment combinations are sorted from best to worst based on the highest “Storage” values, followed by “Sequence lengths” values. Column “Storage” represents the last time we were able to obtain sequences from the specimen in a condition that enables species determination (censoring time 24 months). We were looking for the highest “Sequence lengths” with any value above 500 considered acceptable. The remaining columns represent species identification success rates
| Fixative fluid . | Storage fluid . | Storage (months) . | Sequence length . | GenBank identity score . | % similarity . | E-value . | ID correct . |
|---|---|---|---|---|---|---|---|
| Acetic alcohol | Acetone | 24 | 794 | 785/794 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Acetone | 24 | 789 | 785/789 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | 80% ethanol | 24 | 788 | 787/788 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Absolute ethanol | 24 | 788 | 783/788 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | Acetone | 24 | 788 | 786/788 | 99 | 0.0 | Yes |
| KAA | Acetone | 24 | 787 | 785/787 | 99 | 0.0 | Yes |
| KAA | 70% ethanol | 24 | 782 | 779/783 | 99 | 0.0 | Yes |
| Absolute ethanol cold | 70% ethanol | 24 | 781 | 779/781 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | 70% ethanol | 24 | 780 | 776/780 | 99 | 0.0 | Yes |
| KAA | 80% ethanol | 24 | 780 | 773/780 | 99 | 0.0 | Yes |
| Acetic alcohol | 80% ethanol | 24 | 774 | 768/774 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | Absolute ethanol | 24 | 772 | 767/772 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | Absolute ethanol | 24 | 763 | 760/763 | 99 | 0.0 | Yes |
| Acetic alcohol | Absolute ethanol | 24 | 675 | 669/675 | 99 | 0.0 | Yes |
| Absolute ethanol cold | 80% ethanol | 24 | 674 | 672/674 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | 70% ethanol | 24 | 604 | 599/604 | 99 | 0.0 | Yes |
| KAA | Absolute ethanol | 24 | 604 | 595/604 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | 80% ethanol | 24 | 575 | 571/575 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | 80% ethanol | 24 | 565 | 562/565 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | Acetone | 24 | 553 | 549/553 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | 70% ethanol | 24 | 430 | 426/430 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Absolute ethanol | 24 | 385 | 380/385 | 99 | 0.0 | Yes |
| KAA | Acetic alcohol | 22 | 792 | 785/789 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Acetic alcohol | 22 | 791 | 791/791 | 100 | 0.0 | Yes |
| HWK 80 °C 5 min | AGA | 22 | 391 | 390/391 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Acetone | 22 | 317 | 315/317 | 99 | 5E-159 | Yes |
| Acetic alcohol | 70% ethanol | 22 | 287 | 283/287 | 99 | 4E-139 | Yes |
| Absolute ethanol boiling | AGA | 22 | 125 | 124/125 | 99 | 5E-55 | Yes |
| Acetic alcohol | Acetic alcohol | 20 | 598 | 596/598 | 99 | 0.0 | Yes |
| Acetic alcohol | AGA | 8 | 803 | 800/803 | 99 | s | Yes |
| Absolute ethanol cold | AGA | 8 | 778 | 776/778 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Acetic alcohol | 8 | 754 | 753/754 | 99 | 0.0 | Yes |
| KAA | AGA | 8 | 216 | 209/216 | 99 | 3E-95 | Yes |
| HWK 80 °C 6 h | AGA | 8 | 171 | 167/169 | 99 | 1E-77 | Yes |
| Absolute ethanol boiling | Acetic alcohol | 8 | 105 | 105/105 | 100 | 1E-45 | Yes |
| HWK 80 °C 6 h | Acetic alcohol | 6 | 808 | 803/808 | 99 | 0.0 | Yes |
| Fixative fluid . | Storage fluid . | Storage (months) . | Sequence length . | GenBank identity score . | % similarity . | E-value . | ID correct . |
|---|---|---|---|---|---|---|---|
| Acetic alcohol | Acetone | 24 | 794 | 785/794 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Acetone | 24 | 789 | 785/789 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | 80% ethanol | 24 | 788 | 787/788 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Absolute ethanol | 24 | 788 | 783/788 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | Acetone | 24 | 788 | 786/788 | 99 | 0.0 | Yes |
| KAA | Acetone | 24 | 787 | 785/787 | 99 | 0.0 | Yes |
| KAA | 70% ethanol | 24 | 782 | 779/783 | 99 | 0.0 | Yes |
| Absolute ethanol cold | 70% ethanol | 24 | 781 | 779/781 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | 70% ethanol | 24 | 780 | 776/780 | 99 | 0.0 | Yes |
| KAA | 80% ethanol | 24 | 780 | 773/780 | 99 | 0.0 | Yes |
| Acetic alcohol | 80% ethanol | 24 | 774 | 768/774 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | Absolute ethanol | 24 | 772 | 767/772 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | Absolute ethanol | 24 | 763 | 760/763 | 99 | 0.0 | Yes |
| Acetic alcohol | Absolute ethanol | 24 | 675 | 669/675 | 99 | 0.0 | Yes |
| Absolute ethanol cold | 80% ethanol | 24 | 674 | 672/674 | 99 | 0.0 | Yes |
| Absolute ethanol boiling | 70% ethanol | 24 | 604 | 599/604 | 99 | 0.0 | Yes |
| KAA | Absolute ethanol | 24 | 604 | 595/604 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | 80% ethanol | 24 | 575 | 571/575 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | 80% ethanol | 24 | 565 | 562/565 | 99 | 0.0 | Yes |
| HWK 80 °C 6 h | Acetone | 24 | 553 | 549/553 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | 70% ethanol | 24 | 430 | 426/430 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Absolute ethanol | 24 | 385 | 380/385 | 99 | 0.0 | Yes |
| KAA | Acetic alcohol | 22 | 792 | 785/789 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Acetic alcohol | 22 | 791 | 791/791 | 100 | 0.0 | Yes |
| HWK 80 °C 5 min | AGA | 22 | 391 | 390/391 | 99 | 0.0 | Yes |
| HWK 80 °C 5 min | Acetone | 22 | 317 | 315/317 | 99 | 5E-159 | Yes |
| Acetic alcohol | 70% ethanol | 22 | 287 | 283/287 | 99 | 4E-139 | Yes |
| Absolute ethanol boiling | AGA | 22 | 125 | 124/125 | 99 | 5E-55 | Yes |
| Acetic alcohol | Acetic alcohol | 20 | 598 | 596/598 | 99 | 0.0 | Yes |
| Acetic alcohol | AGA | 8 | 803 | 800/803 | 99 | s | Yes |
| Absolute ethanol cold | AGA | 8 | 778 | 776/778 | 99 | 0.0 | Yes |
| Absolute ethanol cold | Acetic alcohol | 8 | 754 | 753/754 | 99 | 0.0 | Yes |
| KAA | AGA | 8 | 216 | 209/216 | 99 | 3E-95 | Yes |
| HWK 80 °C 6 h | AGA | 8 | 171 | 167/169 | 99 | 1E-77 | Yes |
| Absolute ethanol boiling | Acetic alcohol | 8 | 105 | 105/105 | 100 | 1E-45 | Yes |
| HWK 80 °C 6 h | Acetic alcohol | 6 | 808 | 803/808 | 99 | 0.0 | Yes |
This PDF is available to Subscribers Only
View Article Abstract & Purchase OptionsFor full access to this pdf, sign in to an existing account, or purchase an annual subscription.
