Table 2. Open in new tab Determination... | Oxford Academic

Table 2.

Open in new tab

Determination of the sensitivity by seven serials 10-fold dilutions.

	CT values (SYBR Green)			Ct values (Taq Path kit, ORF1ab probe)
Concentration of RNA Dilution Factor 1/10	Spike del 143-145	N del 31-33	S control
50 ng/μl	19.51 ± 0.354	19.43 ± 0.489	19.07 ± 0.518	17.73 ± 0.154
5 ng/μl	21.89 ± 0.612	21.59 ± 0.342	21.42 ± 0.485	20.79 ± 0.267
0.5 ng/μl	24.99 ± 0.3004	25 ± 0.189	24.65 ± 0.461	24.05 ± 0.251
0.05 ng/µl	28.58 ± 0.436	28.69 ± 0.325	28.34 ± 0.297	27.89 ± 0.153
0.005 ng/ul	30.94 ± 0.640	32.1 ± 0.425	33.52 ± 0.543	31.61 ± 0.075
0.0005 ng/ul	35.56 ± 0.338	35.4 ± 0.120	34.32 ± 0.33	34.05 ± 0.250
0.00005 ng/ul	ND	ND	ND	ND

	CT values (SYBR Green)			Ct values (Taq Path kit, ORF1ab probe)
Concentration of RNA Dilution Factor 1/10	Spike del 143-145	N del 31-33	S control
50 ng/μl	19.51 ± 0.354	19.43 ± 0.489	19.07 ± 0.518	17.73 ± 0.154
5 ng/μl	21.89 ± 0.612	21.59 ± 0.342	21.42 ± 0.485	20.79 ± 0.267
0.5 ng/μl	24.99 ± 0.3004	25 ± 0.189	24.65 ± 0.461	24.05 ± 0.251
0.05 ng/µl	28.58 ± 0.436	28.69 ± 0.325	28.34 ± 0.297	27.89 ± 0.153
0.005 ng/ul	30.94 ± 0.640	32.1 ± 0.425	33.52 ± 0.543	31.61 ± 0.075
0.0005 ng/ul	35.56 ± 0.338	35.4 ± 0.120	34.32 ± 0.33	34.05 ± 0.250
0.00005 ng/ul	ND	ND	ND	ND

Diluted samples were tested by our SYBR Green-based protocol and by TaqMan RT-qPCR (TaqPath kit). ND: not detected.

Table 2.

Open in new tab

Determination of the sensitivity by seven serials 10-fold dilutions.

	CT values (SYBR Green)			Ct values (Taq Path kit, ORF1ab probe)
Concentration of RNA Dilution Factor 1/10	Spike del 143-145	N del 31-33	S control
50 ng/μl	19.51 ± 0.354	19.43 ± 0.489	19.07 ± 0.518	17.73 ± 0.154
5 ng/μl	21.89 ± 0.612	21.59 ± 0.342	21.42 ± 0.485	20.79 ± 0.267
0.5 ng/μl	24.99 ± 0.3004	25 ± 0.189	24.65 ± 0.461	24.05 ± 0.251
0.05 ng/µl	28.58 ± 0.436	28.69 ± 0.325	28.34 ± 0.297	27.89 ± 0.153
0.005 ng/ul	30.94 ± 0.640	32.1 ± 0.425	33.52 ± 0.543	31.61 ± 0.075
0.0005 ng/ul	35.56 ± 0.338	35.4 ± 0.120	34.32 ± 0.33	34.05 ± 0.250
0.00005 ng/ul	ND	ND	ND	ND

	CT values (SYBR Green)			Ct values (Taq Path kit, ORF1ab probe)
Concentration of RNA Dilution Factor 1/10	Spike del 143-145	N del 31-33	S control
50 ng/μl	19.51 ± 0.354	19.43 ± 0.489	19.07 ± 0.518	17.73 ± 0.154
5 ng/μl	21.89 ± 0.612	21.59 ± 0.342	21.42 ± 0.485	20.79 ± 0.267
0.5 ng/μl	24.99 ± 0.3004	25 ± 0.189	24.65 ± 0.461	24.05 ± 0.251
0.05 ng/µl	28.58 ± 0.436	28.69 ± 0.325	28.34 ± 0.297	27.89 ± 0.153
0.005 ng/ul	30.94 ± 0.640	32.1 ± 0.425	33.52 ± 0.543	31.61 ± 0.075
0.0005 ng/ul	35.56 ± 0.338	35.4 ± 0.120	34.32 ± 0.33	34.05 ± 0.250
0.00005 ng/ul	ND	ND	ND	ND

Diluted samples were tested by our SYBR Green-based protocol and by TaqMan RT-qPCR (TaqPath kit). ND: not detected.