| Primary antibodies . | Dilution . | Target retrieval . | Secondary antibodies . | Target . |
|---|---|---|---|---|
| CD15 | 1:1.000 | High PH | Polymer | Neutrophils |
| CD163 | 1:500 | High PH | Polymer | M2 Macrophages |
| CD68 | 1:200 | High PH | Polymer | Macrophages |
| CD3 | 1:500 | pH6.1 | Polymer | T-Lymphocytes |
| CD79a | 1:100 | pH6.1 | Polymer | B-Lymphocytes |
| SMA | 1:300 | Citrat | Anti-mouse | Contracting cells |
| Vimentin | 1:500 | Citrat | Anti-mouse | Somatic cells |
| CD138 | 1:200 | High PH | Polymer | Plasma cells |
| CD31 | 1:100 | High PH | Polymer | Endothelial cells |
| CD45 | 1:3000 | pH6.1 | Polymer | Leucocytes |
| Fibrin | 1:500 | Citrat | Anti-mouse | Fibrin |
| CD42b | 1:600 | High PH | Polymer | Platelets |
| Primary antibodies . | Dilution . | Target retrieval . | Secondary antibodies . | Target . |
|---|---|---|---|---|
| CD15 | 1:1.000 | High PH | Polymer | Neutrophils |
| CD163 | 1:500 | High PH | Polymer | M2 Macrophages |
| CD68 | 1:200 | High PH | Polymer | Macrophages |
| CD3 | 1:500 | pH6.1 | Polymer | T-Lymphocytes |
| CD79a | 1:100 | pH6.1 | Polymer | B-Lymphocytes |
| SMA | 1:300 | Citrat | Anti-mouse | Contracting cells |
| Vimentin | 1:500 | Citrat | Anti-mouse | Somatic cells |
| CD138 | 1:200 | High PH | Polymer | Plasma cells |
| CD31 | 1:100 | High PH | Polymer | Endothelial cells |
| CD45 | 1:3000 | pH6.1 | Polymer | Leucocytes |
| Fibrin | 1:500 | Citrat | Anti-mouse | Fibrin |
| CD42b | 1:600 | High PH | Polymer | Platelets |
Primary antibodies: mouse anti-human CD15 (Zytomed, Berlin, Germany) (MSK108-05); mouse anti-human CD163 (BioRad, Hercules, CA, USA) (MCA1853); mouse anti-human CD68 (Dako, Glostrun, Denmark) (M0876); polyclonal rabbit anti-human CD3 (Dako, A0452); mouse anti-human CD79a (Dako, M7050); mouse anti-human smooth muscle actin (SMA) (Dako, M0851); mouse anti-human CD138 clone MI15 (Dako, M0751); mouse anti-human CD31 (Dako, M0823); mouse anti-human CD45 (Cell Marque, 145M-96); mouse anti-human fibrin ß-chain (Biomedica Diagnostics (Windsor, ON, Canada), ref 350); and rabbit anti-human CD42b (Abcam, PLC, Cambridge, United Kingdom, ab227669). Target retrieval: High pH: EnVision Flex Target Retrieval Solution high pH, (Dako, K8004); pH6.1: target retrieval solution pH6,1 (Dako, S1699); Citrat: Target Retrieval Solution Citrat pH 6 (Dako, S2369). Secondary antibodies: polymer: Zytochem plus horseradish peroxidase/polymer system mouse/rabbit (Zytomed, POLHRP-100); anti-mouse: polyclonal rabbit anti-mouse immunoglobulins/horseradish peroxidase (Dako, P0260).
| Primary antibodies . | Dilution . | Target retrieval . | Secondary antibodies . | Target . |
|---|---|---|---|---|
| CD15 | 1:1.000 | High PH | Polymer | Neutrophils |
| CD163 | 1:500 | High PH | Polymer | M2 Macrophages |
| CD68 | 1:200 | High PH | Polymer | Macrophages |
| CD3 | 1:500 | pH6.1 | Polymer | T-Lymphocytes |
| CD79a | 1:100 | pH6.1 | Polymer | B-Lymphocytes |
| SMA | 1:300 | Citrat | Anti-mouse | Contracting cells |
| Vimentin | 1:500 | Citrat | Anti-mouse | Somatic cells |
| CD138 | 1:200 | High PH | Polymer | Plasma cells |
| CD31 | 1:100 | High PH | Polymer | Endothelial cells |
| CD45 | 1:3000 | pH6.1 | Polymer | Leucocytes |
| Fibrin | 1:500 | Citrat | Anti-mouse | Fibrin |
| CD42b | 1:600 | High PH | Polymer | Platelets |
| Primary antibodies . | Dilution . | Target retrieval . | Secondary antibodies . | Target . |
|---|---|---|---|---|
| CD15 | 1:1.000 | High PH | Polymer | Neutrophils |
| CD163 | 1:500 | High PH | Polymer | M2 Macrophages |
| CD68 | 1:200 | High PH | Polymer | Macrophages |
| CD3 | 1:500 | pH6.1 | Polymer | T-Lymphocytes |
| CD79a | 1:100 | pH6.1 | Polymer | B-Lymphocytes |
| SMA | 1:300 | Citrat | Anti-mouse | Contracting cells |
| Vimentin | 1:500 | Citrat | Anti-mouse | Somatic cells |
| CD138 | 1:200 | High PH | Polymer | Plasma cells |
| CD31 | 1:100 | High PH | Polymer | Endothelial cells |
| CD45 | 1:3000 | pH6.1 | Polymer | Leucocytes |
| Fibrin | 1:500 | Citrat | Anti-mouse | Fibrin |
| CD42b | 1:600 | High PH | Polymer | Platelets |
Primary antibodies: mouse anti-human CD15 (Zytomed, Berlin, Germany) (MSK108-05); mouse anti-human CD163 (BioRad, Hercules, CA, USA) (MCA1853); mouse anti-human CD68 (Dako, Glostrun, Denmark) (M0876); polyclonal rabbit anti-human CD3 (Dako, A0452); mouse anti-human CD79a (Dako, M7050); mouse anti-human smooth muscle actin (SMA) (Dako, M0851); mouse anti-human CD138 clone MI15 (Dako, M0751); mouse anti-human CD31 (Dako, M0823); mouse anti-human CD45 (Cell Marque, 145M-96); mouse anti-human fibrin ß-chain (Biomedica Diagnostics (Windsor, ON, Canada), ref 350); and rabbit anti-human CD42b (Abcam, PLC, Cambridge, United Kingdom, ab227669). Target retrieval: High pH: EnVision Flex Target Retrieval Solution high pH, (Dako, K8004); pH6.1: target retrieval solution pH6,1 (Dako, S1699); Citrat: Target Retrieval Solution Citrat pH 6 (Dako, S2369). Secondary antibodies: polymer: Zytochem plus horseradish peroxidase/polymer system mouse/rabbit (Zytomed, POLHRP-100); anti-mouse: polyclonal rabbit anti-mouse immunoglobulins/horseradish peroxidase (Dako, P0260).
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