Table 3.

Proportion of TDV-2, revertants, and DENV-2 in complex mixtures using UMIs.

RNAComplex Mix 1
Complex Mix 2
Complex Mix 3
Complex Mix 4
UMIa (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)b
P123.273511.73NANANANANANANANANA
P357.76507.7610.84100.849.62100.38NANANA
P56.8951.89NANANANANANANANANA
P51NANANA21.56201.5625.12205.12NANANA
P513NANANA37.16402.8464.89c405.11NANANA
wt DENV-2NANANANANANA3065.57759.43
TDV-211.2101.229.59300.41NANANA33.7258.7
RNAComplex Mix 1
Complex Mix 2
Complex Mix 3
Complex Mix 4
UMIa (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)b
P123.273511.73NANANANANANANANANA
P357.76507.7610.84100.849.62100.38NANANA
P56.8951.89NANANANANANANANANA
P51NANANA21.56201.5625.12205.12NANANA
P513NANANA37.16402.8464.89c405.11NANANA
wt DENV-2NANANANANANA3065.57759.43
TDV-211.2101.229.59300.41NANANA33.7258.7
a

Determined by UMI.

b

Absolute value of Expected % − UMI%.

c

P513 and WT DENV-2 have the same nucleotides at positions 57, 2579, and 5270 and were indistinguishable.

NA, not applicable.

Table 3.

Proportion of TDV-2, revertants, and DENV-2 in complex mixtures using UMIs.

RNAComplex Mix 1
Complex Mix 2
Complex Mix 3
Complex Mix 4
UMIa (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)b
P123.273511.73NANANANANANANANANA
P357.76507.7610.84100.849.62100.38NANANA
P56.8951.89NANANANANANANANANA
P51NANANA21.56201.5625.12205.12NANANA
P513NANANA37.16402.8464.89c405.11NANANA
wt DENV-2NANANANANANA3065.57759.43
TDV-211.2101.229.59300.41NANANA33.7258.7
RNAComplex Mix 1
Complex Mix 2
Complex Mix 3
Complex Mix 4
UMIa (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)bUMI (%)Expected (%)Difference (|E-U|)b
P123.273511.73NANANANANANANANANA
P357.76507.7610.84100.849.62100.38NANANA
P56.8951.89NANANANANANANANANA
P51NANANA21.56201.5625.12205.12NANANA
P513NANANA37.16402.8464.89c405.11NANANA
wt DENV-2NANANANANANA3065.57759.43
TDV-211.2101.229.59300.41NANANA33.7258.7
a

Determined by UMI.

b

Absolute value of Expected % − UMI%.

c

P513 and WT DENV-2 have the same nucleotides at positions 57, 2579, and 5270 and were indistinguishable.

NA, not applicable.

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