Table 2 Open in new tab Bacterial taxa...

	Number of MOTUs from:	Number of pyrosequences from:
Actinobacteria	25	1	161		48	8	155	3	350	2	1685		84	106	578	15
Armatimonadetes					3		1	1					6		1	1
Bacteroidetes	8		36		39	5	66	3	108		208		2483	60	403	8
Verrucomicrobia					2								5
Chloroflexi	1		6				9		4		44				24
Deinococcus-Thermus			7		5		13				26		7		40
Acidobacteria	4		1		1		3	2	112		55		1		30	67
Firmicutes	11		24	1	11	5	24	2	157		250	2	16	30	109	10
Fusobacteria	1		1				2		1		1				2
Gemmatimonadetes					2								2
Nitrospirae					1		1						1		1
Planctomycetes			1		2		2				1		2		4
Proteobacteria
Alpha	41	2	100	8	186	41	168	16	606	11	1154	52	7335	1058	2478	121
Beta	9		22		39	14	46	2	100		464		879	296	525	42
Delta	1		1		3		2		36		5		6			5
Gamma	19	2	26	2	67	23	35	5	357	3	231	6	4784	479	1094	239
Unidentified					9	2	4							155	2	31
Spirochetes	1								1
Unidentified division
SC4				3			4				23				6
TM7			1		1						2		1
WPS-2					2								2
Unclassified bacteria	4		32	5	26	10	36	8	8		112	27	145	165	103	25
Total	122	4	425	17	447	108	571	42	1783	14	4318	89	15914	2196	5434	528

Number of MOTUs from:

Number of pyrosequences from:

Taxa

SAF cleanroom floor (GI-36-4)

SAF–GSE (GI-36-3)

Bldg 144 cleanroom floor (GI-42-1)

Bldg 144 GSE (GI-42-2)

SAF cleanroom floor (GI-36-4)

SAF–GSE (GI-36-3)

Bldg 144 cleanroom floor (GI-42-1)

Bldg 144 GSE (GI-42-2)

As is

PMA

As is

PMA

As is

PMA

As is

PMA

As is

PMA

As is

PMA

As is

PMA

As is

PMA

Actinobacteria

161

155

350

1685

106

578

Armatimonadetes

Bacteroidetes

108

208

2483

403

Verrucomicrobia

Chloroflexi

Deinococcus-Thermus

Acidobacteria

112

Firmicutes

157

250

109

Fusobacteria

Gemmatimonadetes

Nitrospirae

Planctomycetes

Proteobacteria

Alpha

100

186

168

606

1154

7335

1058

2478

121

Beta

100

464

879

296

525

Delta

Gamma

357

231

4784

479

1094

239

Unidentified

155

Spirochetes

Unidentified division

SC4

TM7

WPS-2

Unclassified bacteria

112

145

165

103

Total

122

425

447

108

571

1783

4318

15914

2196

5434

528

	Number of MOTUs from:	Number of pyrosequences from:
Actinobacteria	25	1	161		48	8	155	3	350	2	1685		84	106	578	15
Armatimonadetes					3		1	1					6		1	1
Bacteroidetes	8		36		39	5	66	3	108		208		2483	60	403	8
Verrucomicrobia					2								5
Chloroflexi	1		6				9		4		44				24
Deinococcus-Thermus			7		5		13				26		7		40
Acidobacteria	4		1		1		3	2	112		55		1		30	67
Firmicutes	11		24	1	11	5	24	2	157		250	2	16	30	109	10
Fusobacteria	1		1				2		1		1				2
Gemmatimonadetes					2								2
Nitrospirae					1		1						1		1
Planctomycetes			1		2		2				1		2		4
Proteobacteria
Alpha	41	2	100	8	186	41	168	16	606	11	1154	52	7335	1058	2478	121
Beta	9		22		39	14	46	2	100		464		879	296	525	42
Delta	1		1		3		2		36		5		6			5
Gamma	19	2	26	2	67	23	35	5	357	3	231	6	4784	479	1094	239
Unidentified					9	2	4							155	2	31
Spirochetes	1								1
Unidentified division
SC4				3			4				23				6
TM7			1		1						2		1
WPS-2					2								2
Unclassified bacteria	4		32	5	26	10	36	8	8		112	27	145	165	103	25
Total	122	4	425	17	447	108	571	42	1783	14	4318	89	15914	2196	5434	528

Table 2

Open in new tab

Bacterial taxa present in various cleanroom samples as determined by pyrosequencing method

	Number of MOTUs from:								Number of pyrosequences from:
Taxa	SAF cleanroom floor (GI-36-4)		SAF–GSE (GI-36-3)		Bldg 144 cleanroom floor (GI-42-1)		Bldg 144 GSE (GI-42-2)		SAF cleanroom floor (GI-36-4)		SAF–GSE (GI-36-3)		Bldg 144 cleanroom floor (GI-42-1)		Bldg 144 GSE (GI-42-2)
	As is	PMA	As is	PMA	As is	PMA	As is	PMA	As is	PMA	As is	PMA	As is	PMA	As is	PMA
Actinobacteria	25	1	161		48	8	155	3	350	2	1685		84	106	578	15
Armatimonadetes					3		1	1					6		1	1
Bacteroidetes	8		36		39	5	66	3	108		208		2483	60	403	8
Verrucomicrobia					2								5
Chloroflexi	1		6				9		4		44				24
Deinococcus-Thermus			7		5		13				26		7		40
Acidobacteria	4		1		1		3	2	112		55		1		30	67
Firmicutes	11		24	1	11	5	24	2	157		250	2	16	30	109	10
Fusobacteria	1		1				2		1		1				2
Gemmatimonadetes					2								2
Nitrospirae					1		1						1		1
Planctomycetes			1		2		2				1		2		4
Proteobacteria
Alpha	41	2	100	8	186	41	168	16	606	11	1154	52	7335	1058	2478	121
Beta	9		22		39	14	46	2	100		464		879	296	525	42
Delta	1		1		3		2		36		5		6			5
Gamma	19	2	26	2	67	23	35	5	357	3	231	6	4784	479	1094	239
Unidentified					9	2	4							155	2	31
Spirochetes	1								1
Unidentified division
SC4				3			4				23				6
TM7			1		1						2		1
WPS-2					2								2
Unclassified bacteria	4		32	5	26	10	36	8	8		112	27	145	165	103	25
Total	122	4	425	17	447	108	571	42	1783	14	4318	89	15914	2196	5434	528

	Number of MOTUs from:								Number of pyrosequences from:
Taxa	SAF cleanroom floor (GI-36-4)		SAF–GSE (GI-36-3)		Bldg 144 cleanroom floor (GI-42-1)		Bldg 144 GSE (GI-42-2)		SAF cleanroom floor (GI-36-4)		SAF–GSE (GI-36-3)		Bldg 144 cleanroom floor (GI-42-1)		Bldg 144 GSE (GI-42-2)
	As is	PMA	As is	PMA	As is	PMA	As is	PMA	As is	PMA	As is	PMA	As is	PMA	As is	PMA
Actinobacteria	25	1	161		48	8	155	3	350	2	1685		84	106	578	15
Armatimonadetes					3		1	1					6		1	1
Bacteroidetes	8		36		39	5	66	3	108		208		2483	60	403	8
Verrucomicrobia					2								5
Chloroflexi	1		6				9		4		44				24
Deinococcus-Thermus			7		5		13				26		7		40
Acidobacteria	4		1		1		3	2	112		55		1		30	67
Firmicutes	11		24	1	11	5	24	2	157		250	2	16	30	109	10
Fusobacteria	1		1				2		1		1				2
Gemmatimonadetes					2								2
Nitrospirae					1		1						1		1
Planctomycetes			1		2		2				1		2		4
Proteobacteria
Alpha	41	2	100	8	186	41	168	16	606	11	1154	52	7335	1058	2478	121
Beta	9		22		39	14	46	2	100		464		879	296	525	42
Delta	1		1		3		2		36		5		6			5
Gamma	19	2	26	2	67	23	35	5	357	3	231	6	4784	479	1094	239
Unidentified					9	2	4							155	2	31
Spirochetes	1								1
Unidentified division
SC4				3			4				23				6
TM7			1		1						2		1
WPS-2					2								2
Unclassified bacteria	4		32	5	26	10	36	8	8		112	27	145	165	103	25
Total	122	4	425	17	447	108	571	42	1783	14	4318	89	15914	2196	5434	528

Abbreviations: Bldg, building; GSE, ground support equipment; MOTU, molecular operational taxonomic unit; PMA, propidium monoazide; SAF, spacecraft assembly facility.

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