Table 1

ETNP Ca. Scalindua single-cell amplified genome (SAG) statistics

rRNA genesUrease and cyanase-associatedMarker proteins−anammox metabolism
SAGCompContHeterORFsMbpEst Gen (Mbp)a5Sa16Sa23SbUreAbUreBbUreCbUreDbUreEbUreFbUreGcCynScOcta HZOcHzsAcOcta Hao1cOcta Hao2cOcta Hao3cOcta Hao4cNirScAmtB1bAmtB2cAmtB3
B1431.03.550.07000.742.40+++++
B1731.60.00.08620.912.88++++++
B2123.11.750.04510.451.95+++
C1412.31.8100.07450.62NA+++
C819.70.00.07730.512.62+
E1428.20.00.08000.622.18++
F836.50.00.011010.922.53++++++
G1528.63.940.012511.043.65++++++++++6+
H2043.16.770.011040.982.28++++++2++2++
J1120.51.833.35810.432.11+++
K2127.81.9100.012301.063.80+++++++++
L1431.01.10.09510.792.56++
L2026.65.6100.06970.582.17++
L715.81.80.06950.58NA+++
L939.54.433.313211.122.83+++++
M134.20.00.05800.48NA+++++++++++
M733.32.850.09780.792.36+++++++
N130.00.00.02340.19NA++
N1946.21.10.012431.112.41+++++++++++++
N2250.02.833.316371.252.50++++++++
rRNA genesUrease and cyanase-associatedMarker proteins−anammox metabolism
SAGCompContHeterORFsMbpEst Gen (Mbp)a5Sa16Sa23SbUreAbUreBbUreCbUreDbUreEbUreFbUreGcCynScOcta HZOcHzsAcOcta Hao1cOcta Hao2cOcta Hao3cOcta Hao4cNirScAmtB1bAmtB2cAmtB3
B1431.03.550.07000.742.40+++++
B1731.60.00.08620.912.88++++++
B2123.11.750.04510.451.95+++
C1412.31.8100.07450.62NA+++
C819.70.00.07730.512.62+
E1428.20.00.08000.622.18++
F836.50.00.011010.922.53++++++
G1528.63.940.012511.043.65++++++++++6+
H2043.16.770.011040.982.28++++++2++2++
J1120.51.833.35810.432.11+++
K2127.81.9100.012301.063.80+++++++++
L1431.01.10.09510.792.56++
L2026.65.6100.06970.582.17++
L715.81.80.06950.58NA+++
L939.54.433.313211.122.83+++++
M134.20.00.05800.48NA+++++++++++
M733.32.850.09780.792.36+++++++
N130.00.00.02340.19NA++
N1946.21.10.012431.112.41+++++++++++++
N2250.02.833.316371.252.50++++++++

Comp genome completeness (%) estimated by CheckM

Cont genome contamination (%) estimated by CheckM

Heter strain heterogeneity index (range 1–100) estimated by CheckM

ORFs number of protein-coding open reading frames recovered per SAG

Mbp Mbp of contigs recovered per SAG

Est Gen estimated genome size based on estimated completeness and Mbp of SAG; done only for SAGs with completeness >20% and contamination <5%

Ure urease, UreA gamma subunit, UreB beta subunit, ureC alpha subunit

Cyn cyanate hydratase

Ure urease accesory proteins

Octa Octahaem

Hzo hydrazine oxidoreductase/dehydrogenase, S. profunda homolog scal03295

Hzs hydrazine synthase, S. profunda homolog scal01318

Hao hydroxylamine oxidoreductase. S. produnda Hao1 homolog 00421, S. profunda Hao2 homolog 01317, S. profunda Hao 3 homolog 02116, S. profunda homolog 4 04164

Amt ammonia transporter. S. profunda Amt1 homolog 00587, S. profunda Amt2 homolog 00591, S. profunda Amt3 homolog 00594

NirS cytochrome c nitrite reductase, S. profunda nirS homolog 02098

a

top BLASTN match to Brocadiales-affiliated homolog via queries against the SILVA database

b

top BLASTP match to non-Brocadiales via queries against the NCBI-nr database (i.e., homologs not detected in other Brocadiales)

c

top BLASTP match to Brocadiales-affiliated homolog via queries against the NCBI-nr database+publically available Brocadiales databases

Table 1

ETNP Ca. Scalindua single-cell amplified genome (SAG) statistics

rRNA genesUrease and cyanase-associatedMarker proteins−anammox metabolism
SAGCompContHeterORFsMbpEst Gen (Mbp)a5Sa16Sa23SbUreAbUreBbUreCbUreDbUreEbUreFbUreGcCynScOcta HZOcHzsAcOcta Hao1cOcta Hao2cOcta Hao3cOcta Hao4cNirScAmtB1bAmtB2cAmtB3
B1431.03.550.07000.742.40+++++
B1731.60.00.08620.912.88++++++
B2123.11.750.04510.451.95+++
C1412.31.8100.07450.62NA+++
C819.70.00.07730.512.62+
E1428.20.00.08000.622.18++
F836.50.00.011010.922.53++++++
G1528.63.940.012511.043.65++++++++++6+
H2043.16.770.011040.982.28++++++2++2++
J1120.51.833.35810.432.11+++
K2127.81.9100.012301.063.80+++++++++
L1431.01.10.09510.792.56++
L2026.65.6100.06970.582.17++
L715.81.80.06950.58NA+++
L939.54.433.313211.122.83+++++
M134.20.00.05800.48NA+++++++++++
M733.32.850.09780.792.36+++++++
N130.00.00.02340.19NA++
N1946.21.10.012431.112.41+++++++++++++
N2250.02.833.316371.252.50++++++++
rRNA genesUrease and cyanase-associatedMarker proteins−anammox metabolism
SAGCompContHeterORFsMbpEst Gen (Mbp)a5Sa16Sa23SbUreAbUreBbUreCbUreDbUreEbUreFbUreGcCynScOcta HZOcHzsAcOcta Hao1cOcta Hao2cOcta Hao3cOcta Hao4cNirScAmtB1bAmtB2cAmtB3
B1431.03.550.07000.742.40+++++
B1731.60.00.08620.912.88++++++
B2123.11.750.04510.451.95+++
C1412.31.8100.07450.62NA+++
C819.70.00.07730.512.62+
E1428.20.00.08000.622.18++
F836.50.00.011010.922.53++++++
G1528.63.940.012511.043.65++++++++++6+
H2043.16.770.011040.982.28++++++2++2++
J1120.51.833.35810.432.11+++
K2127.81.9100.012301.063.80+++++++++
L1431.01.10.09510.792.56++
L2026.65.6100.06970.582.17++
L715.81.80.06950.58NA+++
L939.54.433.313211.122.83+++++
M134.20.00.05800.48NA+++++++++++
M733.32.850.09780.792.36+++++++
N130.00.00.02340.19NA++
N1946.21.10.012431.112.41+++++++++++++
N2250.02.833.316371.252.50++++++++

Comp genome completeness (%) estimated by CheckM

Cont genome contamination (%) estimated by CheckM

Heter strain heterogeneity index (range 1–100) estimated by CheckM

ORFs number of protein-coding open reading frames recovered per SAG

Mbp Mbp of contigs recovered per SAG

Est Gen estimated genome size based on estimated completeness and Mbp of SAG; done only for SAGs with completeness >20% and contamination <5%

Ure urease, UreA gamma subunit, UreB beta subunit, ureC alpha subunit

Cyn cyanate hydratase

Ure urease accesory proteins

Octa Octahaem

Hzo hydrazine oxidoreductase/dehydrogenase, S. profunda homolog scal03295

Hzs hydrazine synthase, S. profunda homolog scal01318

Hao hydroxylamine oxidoreductase. S. produnda Hao1 homolog 00421, S. profunda Hao2 homolog 01317, S. profunda Hao 3 homolog 02116, S. profunda homolog 4 04164

Amt ammonia transporter. S. profunda Amt1 homolog 00587, S. profunda Amt2 homolog 00591, S. profunda Amt3 homolog 00594

NirS cytochrome c nitrite reductase, S. profunda nirS homolog 02098

a

top BLASTN match to Brocadiales-affiliated homolog via queries against the SILVA database

b

top BLASTP match to non-Brocadiales via queries against the NCBI-nr database (i.e., homologs not detected in other Brocadiales)

c

top BLASTP match to Brocadiales-affiliated homolog via queries against the NCBI-nr database+publically available Brocadiales databases

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