| Protein . | l-DHFR . | cat-DHFR . |
|---|---|---|
| Km (μM) | 9.4 ± 0.8 | 44.4 ± 3.7 |
| kcat (s−1) | 6.8 ± 0.3 | 2.6 ± 0.1 |
| kcat/Km (M−1 s−1) | (7.1 ± 1.0) × 105 | (5.9 ± 0.8) × 104 |
| KD, DHF (μM) | 2.60 ± 0.63 | 451 ± 189 |
| KD, NADPH (μM) | 1.91 ± 0.21 | 27.4 ± 0.7 |
| KD, THF (μM) | 102 ± 106 | 368 ± 313 |
| Protein . | l-DHFR . | cat-DHFR . |
|---|---|---|
| Km (μM) | 9.4 ± 0.8 | 44.4 ± 3.7 |
| kcat (s−1) | 6.8 ± 0.3 | 2.6 ± 0.1 |
| kcat/Km (M−1 s−1) | (7.1 ± 1.0) × 105 | (5.9 ± 0.8) × 104 |
| KD, DHF (μM) | 2.60 ± 0.63 | 451 ± 189 |
| KD, NADPH (μM) | 1.91 ± 0.21 | 27.4 ± 0.7 |
| KD, THF (μM) | 102 ± 106 | 368 ± 313 |
| Protein . | l-DHFR . | cat-DHFR . |
|---|---|---|
| Km (μM) | 9.4 ± 0.8 | 44.4 ± 3.7 |
| kcat (s−1) | 6.8 ± 0.3 | 2.6 ± 0.1 |
| kcat/Km (M−1 s−1) | (7.1 ± 1.0) × 105 | (5.9 ± 0.8) × 104 |
| KD, DHF (μM) | 2.60 ± 0.63 | 451 ± 189 |
| KD, NADPH (μM) | 1.91 ± 0.21 | 27.4 ± 0.7 |
| KD, THF (μM) | 102 ± 106 | 368 ± 313 |
| Protein . | l-DHFR . | cat-DHFR . |
|---|---|---|
| Km (μM) | 9.4 ± 0.8 | 44.4 ± 3.7 |
| kcat (s−1) | 6.8 ± 0.3 | 2.6 ± 0.1 |
| kcat/Km (M−1 s−1) | (7.1 ± 1.0) × 105 | (5.9 ± 0.8) × 104 |
| KD, DHF (μM) | 2.60 ± 0.63 | 451 ± 189 |
| KD, NADPH (μM) | 1.91 ± 0.21 | 27.4 ± 0.7 |
| KD, THF (μM) | 102 ± 106 | 368 ± 313 |
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