Quantitative μCT Macro/Microarchitecture, Lacunar Occupancy, and Tissue Mineral Density of the Bone Calluses (New Bone) and Intact Tibias
| Parameter | 2 weeks postoperation | 3 weeks postoperation | 6 weeks postoperation |
| Lacunar and canalicular measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 15 |
| Ca.D (1/μm2) | 0.082 ± 0.021 | 0.10 ± 0.033* | 0.151 ± 0.033***,***** |
| <Lc.V> (μm3) | 501 ± 99 | 366 ± 29*** | 319 ± 27***,**** |
| N.Lc/BV (103/mm3) | 20.4 ± 4.3 | 52.4 ± 11.5*** | 42.9 ± 7.5***,**** |
| Lacunar occupancy (%) | 94 ± 3.5 (n = 5) | 94 ± 2.8 (n = 5) | 94 ± 1.5 (n = 5) |
| Intact tibias | n = 15 | n = 15 | n = 15 |
| Ca.D (1/μm2) | 0.179 ± 0.035 | 0.175 ± 0.034 | 0.182 ± 0.039 |
| <Lc.V > (μm3) | 302 ± 34 | 297 ± 29 | 312 ± 26 |
| N.Lc/BV (103/mm3) | 52.7 ± 13.4 | 44.9 ± 11.7 | 47 ± 11.5 |
| Lacunar occupancy (%) | 93 ± 3.2 (n = 5) | 92 ± 0.9 (n = 5) | 90 ± 3.9 (n = 5) |
| Macroscopic measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 15 |
| BV/TV (%) | 36.5 ± 6.0 | 43.9 ± 8.4* | 37.3 ± 9.6 |
| TMD (HA mg/cm3) | 627 ± 44 | 796 ± 26*** | 920 ± 24***,***** |
| Microarchitectural measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 14 |
| Tb.N (1/mm) | 12.8 ± 1.6 | 7.8 ± 1.5*** | 3.3 ± 0.7***,***** |
| Tb.Th (mm) | 0.034 ± 0.0025 | 0.049 ± 0.0053*** | 0.085 ± 0.0159***,***** |
| Tb.Sp (mm) | 0.072 ± 0.0129 | 0.124 ± 0.0251*** | 0.300 ± 0.0526***,***** |
| Tb.Th.SD (mm) | 0.0121 ± 0.0016 | 0.0196 ± 0.0058* | 0.0386 ± 0.0117***,***** |
| Tb.Sp.SD (mm) | 0.479 ± 0.020 | 0.072 ± 0.024* | 0.130 ± 0.031***,***** |
| DA (−) | 1.059 ± 0.014 | 1.213 ± 0.081*** | 1.543 ± 0.140***,***** |
| Conn.D (1/mm3) | 3753 ± 524 | 1537 ± 378*** | 93 ± 58***,***** |
| SMI (−) | 0.895 ± 0.350 | 0.484 ± 0.659 | 1.870 ± 0.756***,***** |
| Parameter | 2 weeks postoperation | 3 weeks postoperation | 6 weeks postoperation |
| Lacunar and canalicular measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 15 |
| Ca.D (1/μm2) | 0.082 ± 0.021 | 0.10 ± 0.033* | 0.151 ± 0.033***,***** |
| <Lc.V> (μm3) | 501 ± 99 | 366 ± 29*** | 319 ± 27***,**** |
| N.Lc/BV (103/mm3) | 20.4 ± 4.3 | 52.4 ± 11.5*** | 42.9 ± 7.5***,**** |
| Lacunar occupancy (%) | 94 ± 3.5 (n = 5) | 94 ± 2.8 (n = 5) | 94 ± 1.5 (n = 5) |
| Intact tibias | n = 15 | n = 15 | n = 15 |
| Ca.D (1/μm2) | 0.179 ± 0.035 | 0.175 ± 0.034 | 0.182 ± 0.039 |
| <Lc.V > (μm3) | 302 ± 34 | 297 ± 29 | 312 ± 26 |
| N.Lc/BV (103/mm3) | 52.7 ± 13.4 | 44.9 ± 11.7 | 47 ± 11.5 |
| Lacunar occupancy (%) | 93 ± 3.2 (n = 5) | 92 ± 0.9 (n = 5) | 90 ± 3.9 (n = 5) |
| Macroscopic measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 15 |
| BV/TV (%) | 36.5 ± 6.0 | 43.9 ± 8.4* | 37.3 ± 9.6 |
| TMD (HA mg/cm3) | 627 ± 44 | 796 ± 26*** | 920 ± 24***,***** |
| Microarchitectural measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 14 |
| Tb.N (1/mm) | 12.8 ± 1.6 | 7.8 ± 1.5*** | 3.3 ± 0.7***,***** |
| Tb.Th (mm) | 0.034 ± 0.0025 | 0.049 ± 0.0053*** | 0.085 ± 0.0159***,***** |
| Tb.Sp (mm) | 0.072 ± 0.0129 | 0.124 ± 0.0251*** | 0.300 ± 0.0526***,***** |
| Tb.Th.SD (mm) | 0.0121 ± 0.0016 | 0.0196 ± 0.0058* | 0.0386 ± 0.0117***,***** |
| Tb.Sp.SD (mm) | 0.479 ± 0.020 | 0.072 ± 0.024* | 0.130 ± 0.031***,***** |
| DA (−) | 1.059 ± 0.014 | 1.213 ± 0.081*** | 1.543 ± 0.140***,***** |
| Conn.D (1/mm3) | 3753 ± 524 | 1537 ± 378*** | 93 ± 58***,***** |
| SMI (−) | 0.895 ± 0.350 | 0.484 ± 0.659 | 1.870 ± 0.756***,***** |
μCT = micro–computed tomography; <Lc.V> = mean lacuna volume; BV/TV = bone volume fraction; Ca.D = canalicular number density; Conn.D = connectivity density; DA = degree of anisotropy; HA, hydroxyapatite; N.Lc/BV = lacunar number density; SD = standard deviation; SMI = structure model index; Tb.N = trabecular number; Tb.Sp.SD = standard deviation of Tb.Sp; Tb.Sp = trabecular separation; Tb.Th.SD = standard deviation of Tb.Th; Tb.Th = trabecular thickness; TMD = tissue mineral density.
p < 0.05 when compared to 2 weeks postoperation.
p < 0.01 when compared to 2 weeks postoperation.
p < 0.001 when compared to 2 weeks postoperation.
p < 0.05 when compared to 3 weeks postoperation.
p < 0.001 when compared to 3 weeks postoperation.
Quantitative μCT Macro/Microarchitecture, Lacunar Occupancy, and Tissue Mineral Density of the Bone Calluses (New Bone) and Intact Tibias
| Parameter | 2 weeks postoperation | 3 weeks postoperation | 6 weeks postoperation |
| Lacunar and canalicular measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 15 |
| Ca.D (1/μm2) | 0.082 ± 0.021 | 0.10 ± 0.033* | 0.151 ± 0.033***,***** |
| <Lc.V> (μm3) | 501 ± 99 | 366 ± 29*** | 319 ± 27***,**** |
| N.Lc/BV (103/mm3) | 20.4 ± 4.3 | 52.4 ± 11.5*** | 42.9 ± 7.5***,**** |
| Lacunar occupancy (%) | 94 ± 3.5 (n = 5) | 94 ± 2.8 (n = 5) | 94 ± 1.5 (n = 5) |
| Intact tibias | n = 15 | n = 15 | n = 15 |
| Ca.D (1/μm2) | 0.179 ± 0.035 | 0.175 ± 0.034 | 0.182 ± 0.039 |
| <Lc.V > (μm3) | 302 ± 34 | 297 ± 29 | 312 ± 26 |
| N.Lc/BV (103/mm3) | 52.7 ± 13.4 | 44.9 ± 11.7 | 47 ± 11.5 |
| Lacunar occupancy (%) | 93 ± 3.2 (n = 5) | 92 ± 0.9 (n = 5) | 90 ± 3.9 (n = 5) |
| Macroscopic measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 15 |
| BV/TV (%) | 36.5 ± 6.0 | 43.9 ± 8.4* | 37.3 ± 9.6 |
| TMD (HA mg/cm3) | 627 ± 44 | 796 ± 26*** | 920 ± 24***,***** |
| Microarchitectural measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 14 |
| Tb.N (1/mm) | 12.8 ± 1.6 | 7.8 ± 1.5*** | 3.3 ± 0.7***,***** |
| Tb.Th (mm) | 0.034 ± 0.0025 | 0.049 ± 0.0053*** | 0.085 ± 0.0159***,***** |
| Tb.Sp (mm) | 0.072 ± 0.0129 | 0.124 ± 0.0251*** | 0.300 ± 0.0526***,***** |
| Tb.Th.SD (mm) | 0.0121 ± 0.0016 | 0.0196 ± 0.0058* | 0.0386 ± 0.0117***,***** |
| Tb.Sp.SD (mm) | 0.479 ± 0.020 | 0.072 ± 0.024* | 0.130 ± 0.031***,***** |
| DA (−) | 1.059 ± 0.014 | 1.213 ± 0.081*** | 1.543 ± 0.140***,***** |
| Conn.D (1/mm3) | 3753 ± 524 | 1537 ± 378*** | 93 ± 58***,***** |
| SMI (−) | 0.895 ± 0.350 | 0.484 ± 0.659 | 1.870 ± 0.756***,***** |
| Parameter | 2 weeks postoperation | 3 weeks postoperation | 6 weeks postoperation |
| Lacunar and canalicular measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 15 |
| Ca.D (1/μm2) | 0.082 ± 0.021 | 0.10 ± 0.033* | 0.151 ± 0.033***,***** |
| <Lc.V> (μm3) | 501 ± 99 | 366 ± 29*** | 319 ± 27***,**** |
| N.Lc/BV (103/mm3) | 20.4 ± 4.3 | 52.4 ± 11.5*** | 42.9 ± 7.5***,**** |
| Lacunar occupancy (%) | 94 ± 3.5 (n = 5) | 94 ± 2.8 (n = 5) | 94 ± 1.5 (n = 5) |
| Intact tibias | n = 15 | n = 15 | n = 15 |
| Ca.D (1/μm2) | 0.179 ± 0.035 | 0.175 ± 0.034 | 0.182 ± 0.039 |
| <Lc.V > (μm3) | 302 ± 34 | 297 ± 29 | 312 ± 26 |
| N.Lc/BV (103/mm3) | 52.7 ± 13.4 | 44.9 ± 11.7 | 47 ± 11.5 |
| Lacunar occupancy (%) | 93 ± 3.2 (n = 5) | 92 ± 0.9 (n = 5) | 90 ± 3.9 (n = 5) |
| Macroscopic measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 15 |
| BV/TV (%) | 36.5 ± 6.0 | 43.9 ± 8.4* | 37.3 ± 9.6 |
| TMD (HA mg/cm3) | 627 ± 44 | 796 ± 26*** | 920 ± 24***,***** |
| Microarchitectural measures, mean ± SD | |||
| Bone calluses | n = 15 | n = 15 | n = 14 |
| Tb.N (1/mm) | 12.8 ± 1.6 | 7.8 ± 1.5*** | 3.3 ± 0.7***,***** |
| Tb.Th (mm) | 0.034 ± 0.0025 | 0.049 ± 0.0053*** | 0.085 ± 0.0159***,***** |
| Tb.Sp (mm) | 0.072 ± 0.0129 | 0.124 ± 0.0251*** | 0.300 ± 0.0526***,***** |
| Tb.Th.SD (mm) | 0.0121 ± 0.0016 | 0.0196 ± 0.0058* | 0.0386 ± 0.0117***,***** |
| Tb.Sp.SD (mm) | 0.479 ± 0.020 | 0.072 ± 0.024* | 0.130 ± 0.031***,***** |
| DA (−) | 1.059 ± 0.014 | 1.213 ± 0.081*** | 1.543 ± 0.140***,***** |
| Conn.D (1/mm3) | 3753 ± 524 | 1537 ± 378*** | 93 ± 58***,***** |
| SMI (−) | 0.895 ± 0.350 | 0.484 ± 0.659 | 1.870 ± 0.756***,***** |
μCT = micro–computed tomography; <Lc.V> = mean lacuna volume; BV/TV = bone volume fraction; Ca.D = canalicular number density; Conn.D = connectivity density; DA = degree of anisotropy; HA, hydroxyapatite; N.Lc/BV = lacunar number density; SD = standard deviation; SMI = structure model index; Tb.N = trabecular number; Tb.Sp.SD = standard deviation of Tb.Sp; Tb.Sp = trabecular separation; Tb.Th.SD = standard deviation of Tb.Th; Tb.Th = trabecular thickness; TMD = tissue mineral density.
p < 0.05 when compared to 2 weeks postoperation.
p < 0.01 when compared to 2 weeks postoperation.
p < 0.001 when compared to 2 weeks postoperation.
p < 0.05 when compared to 3 weeks postoperation.
p < 0.001 when compared to 3 weeks postoperation.
This PDF is available to Subscribers Only
View Article Abstract & Purchase OptionsFor full access to this pdf, sign in to an existing account, or purchase an annual subscription.
