Table 2.

The description of TransBorrow’s parameters.

ParametersDescription
—ref_gtf/-r <string>Combined transcriptome assembled by different tools in GTF format (just combine the different assemblies into a GTF file, combine.gtf)
—ref_genome/-g <string>Reference genome in FASTA format;
—bam/-b <string>BAM file;
—strand/-s <string>
  • Strand-specific RNA-Seq reads orientation.

  • If reads are paired:

  • 1) Use <unstranded> to indicate RNA-seq reads are nonstrand-specific;

  • 2) Use <first> to indicate fr-first-stranded RNA-seq read;

  • 3) Use <second> to indicate fr-second-stranded RNA-seq reads;

  • If reads are single:

  • 1) Use <single_unstranded> to indicate RNA-seq reads are nonstrand-specific;

  • 2) Use <single_forward> to indicate RNA-seq reads forward;

  • 3) Use <single_reverse> to indicate RNA-seq reads reverse;

—min_cov/-c <float>Min coverage of recovered transcripts, default: 1;
—output/-o <string>Output path, default: ./TransBorrow_results/TransBorrow.gtf;
—min_trans_len/-l <int>Min length of recovered transcripts, default: 200;
—cre_num/-n <int>Credible sub_paths assembled by at least this number of tools, default: 2;
—min_seed/-d <float>Min seed coverage for extension, default: 0;
—temp_dir/-T <string>Directory storing temporary files, default: ./TransBorrow_tmp;
—threads/-t <int>Number of threads to launch, default: 1;
—version/-vShow current version of TransBorrow;
—help/-hhelp infomation;
ParametersDescription
—ref_gtf/-r <string>Combined transcriptome assembled by different tools in GTF format (just combine the different assemblies into a GTF file, combine.gtf)
—ref_genome/-g <string>Reference genome in FASTA format;
—bam/-b <string>BAM file;
—strand/-s <string>
  • Strand-specific RNA-Seq reads orientation.

  • If reads are paired:

  • 1) Use <unstranded> to indicate RNA-seq reads are nonstrand-specific;

  • 2) Use <first> to indicate fr-first-stranded RNA-seq read;

  • 3) Use <second> to indicate fr-second-stranded RNA-seq reads;

  • If reads are single:

  • 1) Use <single_unstranded> to indicate RNA-seq reads are nonstrand-specific;

  • 2) Use <single_forward> to indicate RNA-seq reads forward;

  • 3) Use <single_reverse> to indicate RNA-seq reads reverse;

—min_cov/-c <float>Min coverage of recovered transcripts, default: 1;
—output/-o <string>Output path, default: ./TransBorrow_results/TransBorrow.gtf;
—min_trans_len/-l <int>Min length of recovered transcripts, default: 200;
—cre_num/-n <int>Credible sub_paths assembled by at least this number of tools, default: 2;
—min_seed/-d <float>Min seed coverage for extension, default: 0;
—temp_dir/-T <string>Directory storing temporary files, default: ./TransBorrow_tmp;
—threads/-t <int>Number of threads to launch, default: 1;
—version/-vShow current version of TransBorrow;
—help/-hhelp infomation;
Table 2.

The description of TransBorrow’s parameters.

ParametersDescription
—ref_gtf/-r <string>Combined transcriptome assembled by different tools in GTF format (just combine the different assemblies into a GTF file, combine.gtf)
—ref_genome/-g <string>Reference genome in FASTA format;
—bam/-b <string>BAM file;
—strand/-s <string>
  • Strand-specific RNA-Seq reads orientation.

  • If reads are paired:

  • 1) Use <unstranded> to indicate RNA-seq reads are nonstrand-specific;

  • 2) Use <first> to indicate fr-first-stranded RNA-seq read;

  • 3) Use <second> to indicate fr-second-stranded RNA-seq reads;

  • If reads are single:

  • 1) Use <single_unstranded> to indicate RNA-seq reads are nonstrand-specific;

  • 2) Use <single_forward> to indicate RNA-seq reads forward;

  • 3) Use <single_reverse> to indicate RNA-seq reads reverse;

—min_cov/-c <float>Min coverage of recovered transcripts, default: 1;
—output/-o <string>Output path, default: ./TransBorrow_results/TransBorrow.gtf;
—min_trans_len/-l <int>Min length of recovered transcripts, default: 200;
—cre_num/-n <int>Credible sub_paths assembled by at least this number of tools, default: 2;
—min_seed/-d <float>Min seed coverage for extension, default: 0;
—temp_dir/-T <string>Directory storing temporary files, default: ./TransBorrow_tmp;
—threads/-t <int>Number of threads to launch, default: 1;
—version/-vShow current version of TransBorrow;
—help/-hhelp infomation;
ParametersDescription
—ref_gtf/-r <string>Combined transcriptome assembled by different tools in GTF format (just combine the different assemblies into a GTF file, combine.gtf)
—ref_genome/-g <string>Reference genome in FASTA format;
—bam/-b <string>BAM file;
—strand/-s <string>
  • Strand-specific RNA-Seq reads orientation.

  • If reads are paired:

  • 1) Use <unstranded> to indicate RNA-seq reads are nonstrand-specific;

  • 2) Use <first> to indicate fr-first-stranded RNA-seq read;

  • 3) Use <second> to indicate fr-second-stranded RNA-seq reads;

  • If reads are single:

  • 1) Use <single_unstranded> to indicate RNA-seq reads are nonstrand-specific;

  • 2) Use <single_forward> to indicate RNA-seq reads forward;

  • 3) Use <single_reverse> to indicate RNA-seq reads reverse;

—min_cov/-c <float>Min coverage of recovered transcripts, default: 1;
—output/-o <string>Output path, default: ./TransBorrow_results/TransBorrow.gtf;
—min_trans_len/-l <int>Min length of recovered transcripts, default: 200;
—cre_num/-n <int>Credible sub_paths assembled by at least this number of tools, default: 2;
—min_seed/-d <float>Min seed coverage for extension, default: 0;
—temp_dir/-T <string>Directory storing temporary files, default: ./TransBorrow_tmp;
—threads/-t <int>Number of threads to launch, default: 1;
—version/-vShow current version of TransBorrow;
—help/-hhelp infomation;
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