A table outlining the main changes made to the protocol by Jones (2014) to optimize the protocol for tiny pollen samples on cotton swabs
| Parameter . | Our protocol . | Jones’ (2014) protocol . |
|---|---|---|
| Sample starting material | Pollen on a cotton swab | Pollen in water, in other liquids, in or on pollinators, on cellophane tape, on filter paper, and pollen pellets |
| Pollen sample size | Tiny | Small to very large |
| Number of water rinses | 1 | 3 or more |
| Ethanol rinse (yes/no) | No | Yes |
| Volume of acetolysis mixture needed per sample | 1 ml | 1–5 ml |
| Water bath duration | 10 min | 5–25 min |
| Straining | Not necessary | Necessary if pollinator body parts remain following the water bath step |
| Parameter . | Our protocol . | Jones’ (2014) protocol . |
|---|---|---|
| Sample starting material | Pollen on a cotton swab | Pollen in water, in other liquids, in or on pollinators, on cellophane tape, on filter paper, and pollen pellets |
| Pollen sample size | Tiny | Small to very large |
| Number of water rinses | 1 | 3 or more |
| Ethanol rinse (yes/no) | No | Yes |
| Volume of acetolysis mixture needed per sample | 1 ml | 1–5 ml |
| Water bath duration | 10 min | 5–25 min |
| Straining | Not necessary | Necessary if pollinator body parts remain following the water bath step |
A table outlining the main changes made to the protocol by Jones (2014) to optimize the protocol for tiny pollen samples on cotton swabs
| Parameter . | Our protocol . | Jones’ (2014) protocol . |
|---|---|---|
| Sample starting material | Pollen on a cotton swab | Pollen in water, in other liquids, in or on pollinators, on cellophane tape, on filter paper, and pollen pellets |
| Pollen sample size | Tiny | Small to very large |
| Number of water rinses | 1 | 3 or more |
| Ethanol rinse (yes/no) | No | Yes |
| Volume of acetolysis mixture needed per sample | 1 ml | 1–5 ml |
| Water bath duration | 10 min | 5–25 min |
| Straining | Not necessary | Necessary if pollinator body parts remain following the water bath step |
| Parameter . | Our protocol . | Jones’ (2014) protocol . |
|---|---|---|
| Sample starting material | Pollen on a cotton swab | Pollen in water, in other liquids, in or on pollinators, on cellophane tape, on filter paper, and pollen pellets |
| Pollen sample size | Tiny | Small to very large |
| Number of water rinses | 1 | 3 or more |
| Ethanol rinse (yes/no) | No | Yes |
| Volume of acetolysis mixture needed per sample | 1 ml | 1–5 ml |
| Water bath duration | 10 min | 5–25 min |
| Straining | Not necessary | Necessary if pollinator body parts remain following the water bath step |
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