Reported measured fluxes in E.coli under anaerobic conditions (Monk et al. 2016) and GAM values (Monk et al. 2017) together with the calculated adjustments of fluxes and of GAM.
| . | Measurement (std. dev.)/model value . | A: QP flux adjustment . | B: QP flux + GAM adjustment . | C: QP for adjustment of fluxes including separate GAM reaction . |
|---|---|---|---|---|
| Glucose uptake | 16.69 (0.24) | 16.71 | 16.70 | 16.73 |
| Ethanol excretion | 11.22 (0.6) | 11.25 | 11.23 | 11.27 |
| Acetate excretion | 11.71 (1.14) | Unchanged | Unchanged | Unchanged |
| Lactate excretion | 0 (0) | Unchanged | Unchanged | Unchanged |
| Formate excretion | 22.17 (1.69) | 22.25 | 22.20 | 22.31 |
| Succinate excretion | 1.86 (0.04) | 1.862 | 1.861 | 1.863 |
| Growth rate μ | 0.46 (0.02) | 0.261 | Fixed | 0.363 |
| GAM in biomass | 75.38 | Fixed | 29.43 | Not applicable |
| Flux through GAM reactiona (for scenario C only) | 0.46 (0.02) (same as µ) | Not applicable | Not applicable | 0.220 |
| OV | 1.998 | 0.846 | 3.373 |
| . | Measurement (std. dev.)/model value . | A: QP flux adjustment . | B: QP flux + GAM adjustment . | C: QP for adjustment of fluxes including separate GAM reaction . |
|---|---|---|---|---|
| Glucose uptake | 16.69 (0.24) | 16.71 | 16.70 | 16.73 |
| Ethanol excretion | 11.22 (0.6) | 11.25 | 11.23 | 11.27 |
| Acetate excretion | 11.71 (1.14) | Unchanged | Unchanged | Unchanged |
| Lactate excretion | 0 (0) | Unchanged | Unchanged | Unchanged |
| Formate excretion | 22.17 (1.69) | 22.25 | 22.20 | 22.31 |
| Succinate excretion | 1.86 (0.04) | 1.862 | 1.861 | 1.863 |
| Growth rate μ | 0.46 (0.02) | 0.261 | Fixed | 0.363 |
| GAM in biomass | 75.38 | Fixed | 29.43 | Not applicable |
| Flux through GAM reactiona (for scenario C only) | 0.46 (0.02) (same as µ) | Not applicable | Not applicable | 0.220 |
| OV | 1.998 | 0.846 | 3.373 |
Three different scenarios (A, B, and C) were considered for adjusting the measured rates or/and GAM values to make the system feasible. Flux rates are in [mmol/(gDW h)], the growth rate in (1/h) and GAM in (mmol/gDW). OV: objective value of the minimization.
The GAM reaction represents an artificial reaction added in scenario C to represent the GAM demand via a mass-balanced ATP hydrolysis reaction. For further explanation, see text.
Reported measured fluxes in E.coli under anaerobic conditions (Monk et al. 2016) and GAM values (Monk et al. 2017) together with the calculated adjustments of fluxes and of GAM.
| . | Measurement (std. dev.)/model value . | A: QP flux adjustment . | B: QP flux + GAM adjustment . | C: QP for adjustment of fluxes including separate GAM reaction . |
|---|---|---|---|---|
| Glucose uptake | 16.69 (0.24) | 16.71 | 16.70 | 16.73 |
| Ethanol excretion | 11.22 (0.6) | 11.25 | 11.23 | 11.27 |
| Acetate excretion | 11.71 (1.14) | Unchanged | Unchanged | Unchanged |
| Lactate excretion | 0 (0) | Unchanged | Unchanged | Unchanged |
| Formate excretion | 22.17 (1.69) | 22.25 | 22.20 | 22.31 |
| Succinate excretion | 1.86 (0.04) | 1.862 | 1.861 | 1.863 |
| Growth rate μ | 0.46 (0.02) | 0.261 | Fixed | 0.363 |
| GAM in biomass | 75.38 | Fixed | 29.43 | Not applicable |
| Flux through GAM reactiona (for scenario C only) | 0.46 (0.02) (same as µ) | Not applicable | Not applicable | 0.220 |
| OV | 1.998 | 0.846 | 3.373 |
| . | Measurement (std. dev.)/model value . | A: QP flux adjustment . | B: QP flux + GAM adjustment . | C: QP for adjustment of fluxes including separate GAM reaction . |
|---|---|---|---|---|
| Glucose uptake | 16.69 (0.24) | 16.71 | 16.70 | 16.73 |
| Ethanol excretion | 11.22 (0.6) | 11.25 | 11.23 | 11.27 |
| Acetate excretion | 11.71 (1.14) | Unchanged | Unchanged | Unchanged |
| Lactate excretion | 0 (0) | Unchanged | Unchanged | Unchanged |
| Formate excretion | 22.17 (1.69) | 22.25 | 22.20 | 22.31 |
| Succinate excretion | 1.86 (0.04) | 1.862 | 1.861 | 1.863 |
| Growth rate μ | 0.46 (0.02) | 0.261 | Fixed | 0.363 |
| GAM in biomass | 75.38 | Fixed | 29.43 | Not applicable |
| Flux through GAM reactiona (for scenario C only) | 0.46 (0.02) (same as µ) | Not applicable | Not applicable | 0.220 |
| OV | 1.998 | 0.846 | 3.373 |
Three different scenarios (A, B, and C) were considered for adjusting the measured rates or/and GAM values to make the system feasible. Flux rates are in [mmol/(gDW h)], the growth rate in (1/h) and GAM in (mmol/gDW). OV: objective value of the minimization.
The GAM reaction represents an artificial reaction added in scenario C to represent the GAM demand via a mass-balanced ATP hydrolysis reaction. For further explanation, see text.
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