Relative (%) and Absolute Cell Yield of SVF and MSCs After Enzymatic Digestion and Staining for Flow Cytometry Analysis of Emulsified (E) and Emulsified + Filtered (E + F) Samples
. | SVF percentage (%) . | SVF total cell yield (cells/mL) . | MSC percentage (%) . | MSC total cell yield (cells/mL) . |
---|---|---|---|---|
E | 7.67 [0.61] | 1.18 [0.12] × 104 | 0.28 [0.03] | 4.26 [0.50] × 102 |
E + F | 5.93 [0.55] | 0.69 [0.09] × 104 | 0.18 [0.02] | 2.04 [0.03] × 102 |
P-value | .012 | .0009 | .0087 | .0009 |
. | SVF percentage (%) . | SVF total cell yield (cells/mL) . | MSC percentage (%) . | MSC total cell yield (cells/mL) . |
---|---|---|---|---|
E | 7.67 [0.61] | 1.18 [0.12] × 104 | 0.28 [0.03] | 4.26 [0.50] × 102 |
E + F | 5.93 [0.55] | 0.69 [0.09] × 104 | 0.18 [0.02] | 2.04 [0.03] × 102 |
P-value | .012 | .0009 | .0087 | .0009 |
P-values are shown for each outcome for the comparison between E and E + F samples. Data are presented as mean [standard error of mean]. MSC, mesenchymal stem cell; SVF, stromal vascular fraction.
Relative (%) and Absolute Cell Yield of SVF and MSCs After Enzymatic Digestion and Staining for Flow Cytometry Analysis of Emulsified (E) and Emulsified + Filtered (E + F) Samples
. | SVF percentage (%) . | SVF total cell yield (cells/mL) . | MSC percentage (%) . | MSC total cell yield (cells/mL) . |
---|---|---|---|---|
E | 7.67 [0.61] | 1.18 [0.12] × 104 | 0.28 [0.03] | 4.26 [0.50] × 102 |
E + F | 5.93 [0.55] | 0.69 [0.09] × 104 | 0.18 [0.02] | 2.04 [0.03] × 102 |
P-value | .012 | .0009 | .0087 | .0009 |
. | SVF percentage (%) . | SVF total cell yield (cells/mL) . | MSC percentage (%) . | MSC total cell yield (cells/mL) . |
---|---|---|---|---|
E | 7.67 [0.61] | 1.18 [0.12] × 104 | 0.28 [0.03] | 4.26 [0.50] × 102 |
E + F | 5.93 [0.55] | 0.69 [0.09] × 104 | 0.18 [0.02] | 2.04 [0.03] × 102 |
P-value | .012 | .0009 | .0087 | .0009 |
P-values are shown for each outcome for the comparison between E and E + F samples. Data are presented as mean [standard error of mean]. MSC, mesenchymal stem cell; SVF, stromal vascular fraction.
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