Values of parameters of electrical signals in protonema cells after the application of 0.5 mM H2O2 directly/indirectly to the cell or in the basal part of the gametophyte
| . | Membrane potential before stimulation . | Membrane potential at the peak of the response . | Amplitude . | Duration at half of the peak . | Rate of depolarization . |
|---|---|---|---|---|---|
| . | V(0) (mV) . | V(max) (mV) . | A (mV) . | t1/2 (s) . | Rdep (mV/s) . |
| Direct/indirect stimulation (WT) | −155 ± 8 (n = 8) | −89 ± 9 (n = 8) | 66 ± 6 (n = 8) | 403 ± 105 (n = 7) | 1.6 ± 0.6a,d (n = 8) |
| Basal stimulation (WT) | −155 ± 7d,h (n = 8) | −74 ± 4 (n = 8) | 81 ± 4a (n = 8) | 486 ± 100 (n = 8) | 9.3 ± 2.5a,b (n = 8) |
| Direct/indirect stimulation (PpglrKO) | −146 ± 4 (n = 10) | −84 ± 5 (n = 10) | 63 ± 4a (n = 10) | 300 ± 70 (n = 10) | 1.1 ± 0.3b,c (n = 10) |
| Basal stimulation (Ppglr1KO) | −155 ± 2 (n = 10) | −77 ± 3 (n = 10) | 78 ± 3 (n = 10) | 294 ± 25 (n = 9) | 8.9 ± 3c,d (n = 10) |
| . | Membrane potential before stimulation . | Membrane potential at the peak of the response . | Amplitude . | Duration at half of the peak . | Rate of depolarization . |
|---|---|---|---|---|---|
| . | V(0) (mV) . | V(max) (mV) . | A (mV) . | t1/2 (s) . | Rdep (mV/s) . |
| Direct/indirect stimulation (WT) | −155 ± 8 (n = 8) | −89 ± 9 (n = 8) | 66 ± 6 (n = 8) | 403 ± 105 (n = 7) | 1.6 ± 0.6a,d (n = 8) |
| Basal stimulation (WT) | −155 ± 7d,h (n = 8) | −74 ± 4 (n = 8) | 81 ± 4a (n = 8) | 486 ± 100 (n = 8) | 9.3 ± 2.5a,b (n = 8) |
| Direct/indirect stimulation (PpglrKO) | −146 ± 4 (n = 10) | −84 ± 5 (n = 10) | 63 ± 4a (n = 10) | 300 ± 70 (n = 10) | 1.1 ± 0.3b,c (n = 10) |
| Basal stimulation (Ppglr1KO) | −155 ± 2 (n = 10) | −77 ± 3 (n = 10) | 78 ± 3 (n = 10) | 294 ± 25 (n = 9) | 8.9 ± 3c,d (n = 10) |
Direct stimulation and indirect stimulation correspond to the measuring variants presented in Fig. 4. Letters a-d indicate statistically significant differences. Explanations are provided in Table 1.
Letters a-h indicate statistically significant differences.
Values of parameters of electrical signals in protonema cells after the application of 0.5 mM H2O2 directly/indirectly to the cell or in the basal part of the gametophyte
| . | Membrane potential before stimulation . | Membrane potential at the peak of the response . | Amplitude . | Duration at half of the peak . | Rate of depolarization . |
|---|---|---|---|---|---|
| . | V(0) (mV) . | V(max) (mV) . | A (mV) . | t1/2 (s) . | Rdep (mV/s) . |
| Direct/indirect stimulation (WT) | −155 ± 8 (n = 8) | −89 ± 9 (n = 8) | 66 ± 6 (n = 8) | 403 ± 105 (n = 7) | 1.6 ± 0.6a,d (n = 8) |
| Basal stimulation (WT) | −155 ± 7d,h (n = 8) | −74 ± 4 (n = 8) | 81 ± 4a (n = 8) | 486 ± 100 (n = 8) | 9.3 ± 2.5a,b (n = 8) |
| Direct/indirect stimulation (PpglrKO) | −146 ± 4 (n = 10) | −84 ± 5 (n = 10) | 63 ± 4a (n = 10) | 300 ± 70 (n = 10) | 1.1 ± 0.3b,c (n = 10) |
| Basal stimulation (Ppglr1KO) | −155 ± 2 (n = 10) | −77 ± 3 (n = 10) | 78 ± 3 (n = 10) | 294 ± 25 (n = 9) | 8.9 ± 3c,d (n = 10) |
| . | Membrane potential before stimulation . | Membrane potential at the peak of the response . | Amplitude . | Duration at half of the peak . | Rate of depolarization . |
|---|---|---|---|---|---|
| . | V(0) (mV) . | V(max) (mV) . | A (mV) . | t1/2 (s) . | Rdep (mV/s) . |
| Direct/indirect stimulation (WT) | −155 ± 8 (n = 8) | −89 ± 9 (n = 8) | 66 ± 6 (n = 8) | 403 ± 105 (n = 7) | 1.6 ± 0.6a,d (n = 8) |
| Basal stimulation (WT) | −155 ± 7d,h (n = 8) | −74 ± 4 (n = 8) | 81 ± 4a (n = 8) | 486 ± 100 (n = 8) | 9.3 ± 2.5a,b (n = 8) |
| Direct/indirect stimulation (PpglrKO) | −146 ± 4 (n = 10) | −84 ± 5 (n = 10) | 63 ± 4a (n = 10) | 300 ± 70 (n = 10) | 1.1 ± 0.3b,c (n = 10) |
| Basal stimulation (Ppglr1KO) | −155 ± 2 (n = 10) | −77 ± 3 (n = 10) | 78 ± 3 (n = 10) | 294 ± 25 (n = 9) | 8.9 ± 3c,d (n = 10) |
Direct stimulation and indirect stimulation correspond to the measuring variants presented in Fig. 4. Letters a-d indicate statistically significant differences. Explanations are provided in Table 1.
Letters a-h indicate statistically significant differences.
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