Values of electrical signal parameters obtained in leaf cells after the application of 0.5 mM H2O2 in the basal part of the gametophyte
| . | Membrane potential before stimulation . | Membrane potential at the peak of the response . | Amplitude . | Duration at half of the peak . | Rate of depolarization . |
|---|---|---|---|---|---|
| . | V(0) (mV) . | V(max) (mV) . | A (mV) . | t1/2 (s) . | Rdep (mV/s) . |
| Standard (WT) | −154 ± 3a,b,c (n = 19) | −86 ± 3a,b,c (n = 19) | 68 ± 3a,b,c,d (n = 19) | 236 ± 44 (n = 17) | 14.5 ± 1.6a,b,c,d (n = 18) |
| 2 mM La3+ | −130 ± 3b (n = 19) | −128 ± 5a,d (n = 9) | 9 ± 2a,e (n = 9) | 205 ± 25 (n = 3) | 0.5 ± 0.2a,e (n = 8) |
| After washout of 2 mM La3+ | −130 ± 4c (n = 19) | −104 ± 5 (n = 5) | 27 ± 4d (n = 4) | 224 ± 34 (n = 15) | 3.6 ± 0.8c (n = 14) |
| 0.5 mM EDTA | −122 ± 5a,d,e (n = 18) | −115 ± 7c (n = 9) | 13 ± 4b (n = 9) | 126 ± 32 (n = 6) | 1.4 ± 0.7b,f (n = 7) |
| After washout of 0.5 mM | −144 ± 4e (n = 18) | −121 ± 7b (n = 8) | 24 ± 6c (n = 8) | 63 ± 14 (n = 7) | 2.5 ± 0.7d (n = 7) |
| Standard (Ppglr1KO) | −146 ± 3d (n = 26) | −100 ± 4d (n = 26) | 46 ± 3e (n = 26) | 194 ± 21 (n = 25) | 9.6 ± 1.2e,f (n = 24) |
| . | Membrane potential before stimulation . | Membrane potential at the peak of the response . | Amplitude . | Duration at half of the peak . | Rate of depolarization . |
|---|---|---|---|---|---|
| . | V(0) (mV) . | V(max) (mV) . | A (mV) . | t1/2 (s) . | Rdep (mV/s) . |
| Standard (WT) | −154 ± 3a,b,c (n = 19) | −86 ± 3a,b,c (n = 19) | 68 ± 3a,b,c,d (n = 19) | 236 ± 44 (n = 17) | 14.5 ± 1.6a,b,c,d (n = 18) |
| 2 mM La3+ | −130 ± 3b (n = 19) | −128 ± 5a,d (n = 9) | 9 ± 2a,e (n = 9) | 205 ± 25 (n = 3) | 0.5 ± 0.2a,e (n = 8) |
| After washout of 2 mM La3+ | −130 ± 4c (n = 19) | −104 ± 5 (n = 5) | 27 ± 4d (n = 4) | 224 ± 34 (n = 15) | 3.6 ± 0.8c (n = 14) |
| 0.5 mM EDTA | −122 ± 5a,d,e (n = 18) | −115 ± 7c (n = 9) | 13 ± 4b (n = 9) | 126 ± 32 (n = 6) | 1.4 ± 0.7b,f (n = 7) |
| After washout of 0.5 mM | −144 ± 4e (n = 18) | −121 ± 7b (n = 8) | 24 ± 6c (n = 8) | 63 ± 14 (n = 7) | 2.5 ± 0.7d (n = 7) |
| Standard (Ppglr1KO) | −146 ± 3d (n = 26) | −100 ± 4d (n = 26) | 46 ± 3e (n = 26) | 194 ± 21 (n = 25) | 9.6 ± 1.2e,f (n = 24) |
The responses recorded in the WT were obtained in the standard solution or the standard solution supplemented with 2 mM LaCl3 or 0.5 mM EDTA. The P. patens knock-out mutants (Ppglr1KO) were tested in the standard solution. Explanations are provided in Table 1.
Letters a-f indicate statistically significant differences.
Values of electrical signal parameters obtained in leaf cells after the application of 0.5 mM H2O2 in the basal part of the gametophyte
| . | Membrane potential before stimulation . | Membrane potential at the peak of the response . | Amplitude . | Duration at half of the peak . | Rate of depolarization . |
|---|---|---|---|---|---|
| . | V(0) (mV) . | V(max) (mV) . | A (mV) . | t1/2 (s) . | Rdep (mV/s) . |
| Standard (WT) | −154 ± 3a,b,c (n = 19) | −86 ± 3a,b,c (n = 19) | 68 ± 3a,b,c,d (n = 19) | 236 ± 44 (n = 17) | 14.5 ± 1.6a,b,c,d (n = 18) |
| 2 mM La3+ | −130 ± 3b (n = 19) | −128 ± 5a,d (n = 9) | 9 ± 2a,e (n = 9) | 205 ± 25 (n = 3) | 0.5 ± 0.2a,e (n = 8) |
| After washout of 2 mM La3+ | −130 ± 4c (n = 19) | −104 ± 5 (n = 5) | 27 ± 4d (n = 4) | 224 ± 34 (n = 15) | 3.6 ± 0.8c (n = 14) |
| 0.5 mM EDTA | −122 ± 5a,d,e (n = 18) | −115 ± 7c (n = 9) | 13 ± 4b (n = 9) | 126 ± 32 (n = 6) | 1.4 ± 0.7b,f (n = 7) |
| After washout of 0.5 mM | −144 ± 4e (n = 18) | −121 ± 7b (n = 8) | 24 ± 6c (n = 8) | 63 ± 14 (n = 7) | 2.5 ± 0.7d (n = 7) |
| Standard (Ppglr1KO) | −146 ± 3d (n = 26) | −100 ± 4d (n = 26) | 46 ± 3e (n = 26) | 194 ± 21 (n = 25) | 9.6 ± 1.2e,f (n = 24) |
| . | Membrane potential before stimulation . | Membrane potential at the peak of the response . | Amplitude . | Duration at half of the peak . | Rate of depolarization . |
|---|---|---|---|---|---|
| . | V(0) (mV) . | V(max) (mV) . | A (mV) . | t1/2 (s) . | Rdep (mV/s) . |
| Standard (WT) | −154 ± 3a,b,c (n = 19) | −86 ± 3a,b,c (n = 19) | 68 ± 3a,b,c,d (n = 19) | 236 ± 44 (n = 17) | 14.5 ± 1.6a,b,c,d (n = 18) |
| 2 mM La3+ | −130 ± 3b (n = 19) | −128 ± 5a,d (n = 9) | 9 ± 2a,e (n = 9) | 205 ± 25 (n = 3) | 0.5 ± 0.2a,e (n = 8) |
| After washout of 2 mM La3+ | −130 ± 4c (n = 19) | −104 ± 5 (n = 5) | 27 ± 4d (n = 4) | 224 ± 34 (n = 15) | 3.6 ± 0.8c (n = 14) |
| 0.5 mM EDTA | −122 ± 5a,d,e (n = 18) | −115 ± 7c (n = 9) | 13 ± 4b (n = 9) | 126 ± 32 (n = 6) | 1.4 ± 0.7b,f (n = 7) |
| After washout of 0.5 mM | −144 ± 4e (n = 18) | −121 ± 7b (n = 8) | 24 ± 6c (n = 8) | 63 ± 14 (n = 7) | 2.5 ± 0.7d (n = 7) |
| Standard (Ppglr1KO) | −146 ± 3d (n = 26) | −100 ± 4d (n = 26) | 46 ± 3e (n = 26) | 194 ± 21 (n = 25) | 9.6 ± 1.2e,f (n = 24) |
The responses recorded in the WT were obtained in the standard solution or the standard solution supplemented with 2 mM LaCl3 or 0.5 mM EDTA. The P. patens knock-out mutants (Ppglr1KO) were tested in the standard solution. Explanations are provided in Table 1.
Letters a-f indicate statistically significant differences.
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