Serum biochemical parameters, aortic calcium content, gene markers of vascular calcification and aortic miRs expression in rats (Phase 2).
| . | . | Reference . | CRF + NP . | CRF + HP . |
|---|---|---|---|---|
| Serum biochemical parameters | Creatinine clearance (mL/min) (mean ± SD) | 2.78 ± 0.55 | 0.99 ± 0.15 *** | 0.48 ± 0.25***## |
| Ca (mg/dL) [median (IQR)] | 10.15 (10.03–10.35) | 10.50 (10.20–10.65) | 9.55 (9.43–9.83) *### | |
| P (mg/dL) [median (IQR)] | 4.10 (4.10–4.25) | 4.80 (4.35–4.85) | 10.30 (6.23–15.58)***# | |
| FGF23 (mg/dL) [median (IQR)] | 274.10 (224.50–362.10) | 427.60 (411.30–463.40) | 1176.30 (1113.70–1200.00)***# | |
| PTH (pg/dL) [median (IQR)] | 647.5 (372.2–1096.8) | 988.0 (842.5–1533.0) | 15 176 (6826–16 869)***## | |
| Aortic osteogenic differentiation | Ca content (µg Ca/mg protein) (median [IQR]) | 8.75 (7.14–10.59) | 36.33 (22.72–60.11) | 155.33 (66.94–396.39)** |
| mRNA Runx2 (RU) [median (IQR)] | 0.97 (0.80–1.18) | 1.97 (1.66–2.31) | 6.69 (3.67–8.35)*** | |
| mRNA Osx (RU) [median (IQR)] | 1.13 (0.79–1.22) | 1.18 (1.04–1.56) | 1.67 (1.61–2.04)** | |
| mRNA α-actin (RU) (mean ± SD) | 1.00 ± 0.37 | 0.28 ± 0.27** | 0.38 ± 0.21** | |
| Aortic miR expression | miR-145/U6 (RU) [median (IQR)] | 0.82 (0.67–1.37) | 0.34 (0.26–0.39)** | 0.31 (0.15–0.36)** |
| miR-122–5p/U6 (RU) [median (IQR)] | 0.83 (0.79–1.12) | 0.03 (0.02–0.10)* | 0.05 (0.05–0.07)* | |
| miR-486/U6 (RU) [median (IQR)] | 1.05 (0.74–1.05) | 0.41 (0.28–0.55)* | 0.16 (0.14–0.18)** | |
| miR-598–3p/U6 (RU) [median (IQR)] | 0.90 (0.57–1.35) | 0.33 (0.16–0.55) | 0.07 (0.01, 0.36)** |
| . | . | Reference . | CRF + NP . | CRF + HP . |
|---|---|---|---|---|
| Serum biochemical parameters | Creatinine clearance (mL/min) (mean ± SD) | 2.78 ± 0.55 | 0.99 ± 0.15 *** | 0.48 ± 0.25***## |
| Ca (mg/dL) [median (IQR)] | 10.15 (10.03–10.35) | 10.50 (10.20–10.65) | 9.55 (9.43–9.83) *### | |
| P (mg/dL) [median (IQR)] | 4.10 (4.10–4.25) | 4.80 (4.35–4.85) | 10.30 (6.23–15.58)***# | |
| FGF23 (mg/dL) [median (IQR)] | 274.10 (224.50–362.10) | 427.60 (411.30–463.40) | 1176.30 (1113.70–1200.00)***# | |
| PTH (pg/dL) [median (IQR)] | 647.5 (372.2–1096.8) | 988.0 (842.5–1533.0) | 15 176 (6826–16 869)***## | |
| Aortic osteogenic differentiation | Ca content (µg Ca/mg protein) (median [IQR]) | 8.75 (7.14–10.59) | 36.33 (22.72–60.11) | 155.33 (66.94–396.39)** |
| mRNA Runx2 (RU) [median (IQR)] | 0.97 (0.80–1.18) | 1.97 (1.66–2.31) | 6.69 (3.67–8.35)*** | |
| mRNA Osx (RU) [median (IQR)] | 1.13 (0.79–1.22) | 1.18 (1.04–1.56) | 1.67 (1.61–2.04)** | |
| mRNA α-actin (RU) (mean ± SD) | 1.00 ± 0.37 | 0.28 ± 0.27** | 0.38 ± 0.21** | |
| Aortic miR expression | miR-145/U6 (RU) [median (IQR)] | 0.82 (0.67–1.37) | 0.34 (0.26–0.39)** | 0.31 (0.15–0.36)** |
| miR-122–5p/U6 (RU) [median (IQR)] | 0.83 (0.79–1.12) | 0.03 (0.02–0.10)* | 0.05 (0.05–0.07)* | |
| miR-486/U6 (RU) [median (IQR)] | 1.05 (0.74–1.05) | 0.41 (0.28–0.55)* | 0.16 (0.14–0.18)** | |
| miR-598–3p/U6 (RU) [median (IQR)] | 0.90 (0.57–1.35) | 0.33 (0.16–0.55) | 0.07 (0.01, 0.36)** |
Reference, sham-operated rats. Data represent median [interquartile range (IQR)] or mean ± standard deviation (SD).
*P < .05, **P < .01, ***P < .001 versus Reference; #P < .05, ##P < .01, ###P < .001 versus CRF + NP.
Serum biochemical parameters, aortic calcium content, gene markers of vascular calcification and aortic miRs expression in rats (Phase 2).
| . | . | Reference . | CRF + NP . | CRF + HP . |
|---|---|---|---|---|
| Serum biochemical parameters | Creatinine clearance (mL/min) (mean ± SD) | 2.78 ± 0.55 | 0.99 ± 0.15 *** | 0.48 ± 0.25***## |
| Ca (mg/dL) [median (IQR)] | 10.15 (10.03–10.35) | 10.50 (10.20–10.65) | 9.55 (9.43–9.83) *### | |
| P (mg/dL) [median (IQR)] | 4.10 (4.10–4.25) | 4.80 (4.35–4.85) | 10.30 (6.23–15.58)***# | |
| FGF23 (mg/dL) [median (IQR)] | 274.10 (224.50–362.10) | 427.60 (411.30–463.40) | 1176.30 (1113.70–1200.00)***# | |
| PTH (pg/dL) [median (IQR)] | 647.5 (372.2–1096.8) | 988.0 (842.5–1533.0) | 15 176 (6826–16 869)***## | |
| Aortic osteogenic differentiation | Ca content (µg Ca/mg protein) (median [IQR]) | 8.75 (7.14–10.59) | 36.33 (22.72–60.11) | 155.33 (66.94–396.39)** |
| mRNA Runx2 (RU) [median (IQR)] | 0.97 (0.80–1.18) | 1.97 (1.66–2.31) | 6.69 (3.67–8.35)*** | |
| mRNA Osx (RU) [median (IQR)] | 1.13 (0.79–1.22) | 1.18 (1.04–1.56) | 1.67 (1.61–2.04)** | |
| mRNA α-actin (RU) (mean ± SD) | 1.00 ± 0.37 | 0.28 ± 0.27** | 0.38 ± 0.21** | |
| Aortic miR expression | miR-145/U6 (RU) [median (IQR)] | 0.82 (0.67–1.37) | 0.34 (0.26–0.39)** | 0.31 (0.15–0.36)** |
| miR-122–5p/U6 (RU) [median (IQR)] | 0.83 (0.79–1.12) | 0.03 (0.02–0.10)* | 0.05 (0.05–0.07)* | |
| miR-486/U6 (RU) [median (IQR)] | 1.05 (0.74–1.05) | 0.41 (0.28–0.55)* | 0.16 (0.14–0.18)** | |
| miR-598–3p/U6 (RU) [median (IQR)] | 0.90 (0.57–1.35) | 0.33 (0.16–0.55) | 0.07 (0.01, 0.36)** |
| . | . | Reference . | CRF + NP . | CRF + HP . |
|---|---|---|---|---|
| Serum biochemical parameters | Creatinine clearance (mL/min) (mean ± SD) | 2.78 ± 0.55 | 0.99 ± 0.15 *** | 0.48 ± 0.25***## |
| Ca (mg/dL) [median (IQR)] | 10.15 (10.03–10.35) | 10.50 (10.20–10.65) | 9.55 (9.43–9.83) *### | |
| P (mg/dL) [median (IQR)] | 4.10 (4.10–4.25) | 4.80 (4.35–4.85) | 10.30 (6.23–15.58)***# | |
| FGF23 (mg/dL) [median (IQR)] | 274.10 (224.50–362.10) | 427.60 (411.30–463.40) | 1176.30 (1113.70–1200.00)***# | |
| PTH (pg/dL) [median (IQR)] | 647.5 (372.2–1096.8) | 988.0 (842.5–1533.0) | 15 176 (6826–16 869)***## | |
| Aortic osteogenic differentiation | Ca content (µg Ca/mg protein) (median [IQR]) | 8.75 (7.14–10.59) | 36.33 (22.72–60.11) | 155.33 (66.94–396.39)** |
| mRNA Runx2 (RU) [median (IQR)] | 0.97 (0.80–1.18) | 1.97 (1.66–2.31) | 6.69 (3.67–8.35)*** | |
| mRNA Osx (RU) [median (IQR)] | 1.13 (0.79–1.22) | 1.18 (1.04–1.56) | 1.67 (1.61–2.04)** | |
| mRNA α-actin (RU) (mean ± SD) | 1.00 ± 0.37 | 0.28 ± 0.27** | 0.38 ± 0.21** | |
| Aortic miR expression | miR-145/U6 (RU) [median (IQR)] | 0.82 (0.67–1.37) | 0.34 (0.26–0.39)** | 0.31 (0.15–0.36)** |
| miR-122–5p/U6 (RU) [median (IQR)] | 0.83 (0.79–1.12) | 0.03 (0.02–0.10)* | 0.05 (0.05–0.07)* | |
| miR-486/U6 (RU) [median (IQR)] | 1.05 (0.74–1.05) | 0.41 (0.28–0.55)* | 0.16 (0.14–0.18)** | |
| miR-598–3p/U6 (RU) [median (IQR)] | 0.90 (0.57–1.35) | 0.33 (0.16–0.55) | 0.07 (0.01, 0.36)** |
Reference, sham-operated rats. Data represent median [interquartile range (IQR)] or mean ± standard deviation (SD).
*P < .05, **P < .01, ***P < .001 versus Reference; #P < .05, ##P < .01, ###P < .001 versus CRF + NP.
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