| RNA molecule . | Mutant phenotype . | Mutant description . | Energy score (kT units) . | Energy score differencea (kT units) . | Relative translationb (%) . | Data source . |
|---|---|---|---|---|---|---|
| Pre-tRNAIle | – | Wild-type | −95514.9 | – | – | Adams et al., 2004 |
| Pre-tRNAIle | Destabilizing | A190U | −95247.5 | 267.4 | – | Chauhan and Woodson, 2008 |
| Pre-tRNAIle | Destabilizing | A190U/A191G | −95282.7 | 232.2 | – | |
| Pre-tRNAIle | Compensatory | C146A/G164U | −95470.3 | 44.6 | – | Costa and Michel, 1997 |
| Pre-tRNAIle | Compensatory | C146G/G164U | −95614.1 | −99.2 | – | |
| Pre-tRNAIle | Compensatory | C146U/G164A | −95427.4 | 87.5 | – | |
| Pre-tRNAIle | Compensatory | G151A/U160A | −95479.0 | 35.9 | – | |
| Pre-tRNAIle | Compensatory | G151A/U160C | −95417.2 | 97.7 | – | |
| HCV-IIIc-loop-IRES | – | Wild-type | −1998.3 | – | 100 | Rijnbrand et al., 2004 |
| HCV-IIIc-loop-IRES | Increased activity | G3A-C8U | −1967.3 | 31.1 | 115 | |
| HCV-IIIc-loop-IRES | Increased activity | C4U-G7A | −2151.2 | −152.9 | 140 | |
| HCV-IIIc-loop-IRES | Increased activity | C4A-G7U | −2147.9 | −149.6 | 140 | |
| HCV-IIIc-loop-IRES | Increased activity | G5A | −2117.6 | −119.3 | 110 | |
| HCV-IIIc-loop-IRES | Increased activity | U6A | −2266.4 | −268.0 | 150 |
| RNA molecule . | Mutant phenotype . | Mutant description . | Energy score (kT units) . | Energy score differencea (kT units) . | Relative translationb (%) . | Data source . |
|---|---|---|---|---|---|---|
| Pre-tRNAIle | – | Wild-type | −95514.9 | – | – | Adams et al., 2004 |
| Pre-tRNAIle | Destabilizing | A190U | −95247.5 | 267.4 | – | Chauhan and Woodson, 2008 |
| Pre-tRNAIle | Destabilizing | A190U/A191G | −95282.7 | 232.2 | – | |
| Pre-tRNAIle | Compensatory | C146A/G164U | −95470.3 | 44.6 | – | Costa and Michel, 1997 |
| Pre-tRNAIle | Compensatory | C146G/G164U | −95614.1 | −99.2 | – | |
| Pre-tRNAIle | Compensatory | C146U/G164A | −95427.4 | 87.5 | – | |
| Pre-tRNAIle | Compensatory | G151A/U160A | −95479.0 | 35.9 | – | |
| Pre-tRNAIle | Compensatory | G151A/U160C | −95417.2 | 97.7 | – | |
| HCV-IIIc-loop-IRES | – | Wild-type | −1998.3 | – | 100 | Rijnbrand et al., 2004 |
| HCV-IIIc-loop-IRES | Increased activity | G3A-C8U | −1967.3 | 31.1 | 115 | |
| HCV-IIIc-loop-IRES | Increased activity | C4U-G7A | −2151.2 | −152.9 | 140 | |
| HCV-IIIc-loop-IRES | Increased activity | C4A-G7U | −2147.9 | −149.6 | 140 | |
| HCV-IIIc-loop-IRES | Increased activity | G5A | −2117.6 | −119.3 | 110 | |
| HCV-IIIc-loop-IRES | Increased activity | U6A | −2266.4 | −268.0 | 150 |
aThe total RASP-ALL energy score of the wild-type structure was subtracted from the total energy score of the mutant.
bThese values were extracted from Figures 7B, C and D in Rijnbrand et al., 2004.
| RNA molecule . | Mutant phenotype . | Mutant description . | Energy score (kT units) . | Energy score differencea (kT units) . | Relative translationb (%) . | Data source . |
|---|---|---|---|---|---|---|
| Pre-tRNAIle | – | Wild-type | −95514.9 | – | – | Adams et al., 2004 |
| Pre-tRNAIle | Destabilizing | A190U | −95247.5 | 267.4 | – | Chauhan and Woodson, 2008 |
| Pre-tRNAIle | Destabilizing | A190U/A191G | −95282.7 | 232.2 | – | |
| Pre-tRNAIle | Compensatory | C146A/G164U | −95470.3 | 44.6 | – | Costa and Michel, 1997 |
| Pre-tRNAIle | Compensatory | C146G/G164U | −95614.1 | −99.2 | – | |
| Pre-tRNAIle | Compensatory | C146U/G164A | −95427.4 | 87.5 | – | |
| Pre-tRNAIle | Compensatory | G151A/U160A | −95479.0 | 35.9 | – | |
| Pre-tRNAIle | Compensatory | G151A/U160C | −95417.2 | 97.7 | – | |
| HCV-IIIc-loop-IRES | – | Wild-type | −1998.3 | – | 100 | Rijnbrand et al., 2004 |
| HCV-IIIc-loop-IRES | Increased activity | G3A-C8U | −1967.3 | 31.1 | 115 | |
| HCV-IIIc-loop-IRES | Increased activity | C4U-G7A | −2151.2 | −152.9 | 140 | |
| HCV-IIIc-loop-IRES | Increased activity | C4A-G7U | −2147.9 | −149.6 | 140 | |
| HCV-IIIc-loop-IRES | Increased activity | G5A | −2117.6 | −119.3 | 110 | |
| HCV-IIIc-loop-IRES | Increased activity | U6A | −2266.4 | −268.0 | 150 |
| RNA molecule . | Mutant phenotype . | Mutant description . | Energy score (kT units) . | Energy score differencea (kT units) . | Relative translationb (%) . | Data source . |
|---|---|---|---|---|---|---|
| Pre-tRNAIle | – | Wild-type | −95514.9 | – | – | Adams et al., 2004 |
| Pre-tRNAIle | Destabilizing | A190U | −95247.5 | 267.4 | – | Chauhan and Woodson, 2008 |
| Pre-tRNAIle | Destabilizing | A190U/A191G | −95282.7 | 232.2 | – | |
| Pre-tRNAIle | Compensatory | C146A/G164U | −95470.3 | 44.6 | – | Costa and Michel, 1997 |
| Pre-tRNAIle | Compensatory | C146G/G164U | −95614.1 | −99.2 | – | |
| Pre-tRNAIle | Compensatory | C146U/G164A | −95427.4 | 87.5 | – | |
| Pre-tRNAIle | Compensatory | G151A/U160A | −95479.0 | 35.9 | – | |
| Pre-tRNAIle | Compensatory | G151A/U160C | −95417.2 | 97.7 | – | |
| HCV-IIIc-loop-IRES | – | Wild-type | −1998.3 | – | 100 | Rijnbrand et al., 2004 |
| HCV-IIIc-loop-IRES | Increased activity | G3A-C8U | −1967.3 | 31.1 | 115 | |
| HCV-IIIc-loop-IRES | Increased activity | C4U-G7A | −2151.2 | −152.9 | 140 | |
| HCV-IIIc-loop-IRES | Increased activity | C4A-G7U | −2147.9 | −149.6 | 140 | |
| HCV-IIIc-loop-IRES | Increased activity | G5A | −2117.6 | −119.3 | 110 | |
| HCV-IIIc-loop-IRES | Increased activity | U6A | −2266.4 | −268.0 | 150 |
aThe total RASP-ALL energy score of the wild-type structure was subtracted from the total energy score of the mutant.
bThese values were extracted from Figures 7B, C and D in Rijnbrand et al., 2004.
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