| . | ST-OTO . | ST-Control . | ICSI-Control . | |
|---|---|---|---|---|
| Injected oocytes | 46 | 39 | 70 | |
| Normally fertilized (2PN)—Day 1 | 29/46 (63%)*a | 24/39 (61.5%)*b | 59/70 (84.2%) | |
| Abnormally fertilized | 3PN | 1/46 (2.1%) | 4/39 (10.2%) | 1/70 (1.4%) |
| 4PN | – | 1/39 (2.5%)*c | – | |
| Direct cleavage | 3/46 (6.5%) | 3/39 (7.6%) | – | |
| 2-cells stage—Day 2 | 19/29 (65.5%) | 17/24 (70.8%) | 47/59 (79.6%) | |
| 4–8 cells stage—Day 3 | 13/29 (44.8%)*d | 16/24 (66.6%) | 42/59 (71.1%) | |
| Blastocyst stage—Day 5 | 6/29 (20.6%) | 7/24 (29.1%) | 15/59 (25.4%) | |
| . | ST-OTO . | ST-Control . | ICSI-Control . | |
|---|---|---|---|---|
| Injected oocytes | 46 | 39 | 70 | |
| Normally fertilized (2PN)—Day 1 | 29/46 (63%)*a | 24/39 (61.5%)*b | 59/70 (84.2%) | |
| Abnormally fertilized | 3PN | 1/46 (2.1%) | 4/39 (10.2%) | 1/70 (1.4%) |
| 4PN | – | 1/39 (2.5%)*c | – | |
| Direct cleavage | 3/46 (6.5%) | 3/39 (7.6%) | – | |
| 2-cells stage—Day 2 | 19/29 (65.5%) | 17/24 (70.8%) | 47/59 (79.6%) | |
| 4–8 cells stage—Day 3 | 13/29 (44.8%)*d | 16/24 (66.6%) | 42/59 (71.1%) | |
| Blastocyst stage—Day 5 | 6/29 (20.6%) | 7/24 (29.1%) | 15/59 (25.4%) | |
OTO, ovarian tissue oocytes collected (in cold medium) from transgender men and matured in vitro; control oocytes, immature oocytes or mature oocytes (with aggregates of smooth endoplasmic reticulum) collected from ovarian stimulated women undergoing IVF; ST, spindle transfer; PN, pronuclei.
Fertilization was performed using ICSI with a single sperm cell. Blastocyst rates were calculated based on the number of normally fertilized zygotes. Percentages were compared using the chi-square test and P < 0.05 was considered significant. Asterisks represent a P value <0.05 between ST-OTO, Control-ST, and ICSI-Control oocytes.
P = 0.009.
P = 0.008.
P < 0.01.
P = 0.016.
| . | ST-OTO . | ST-Control . | ICSI-Control . | |
|---|---|---|---|---|
| Injected oocytes | 46 | 39 | 70 | |
| Normally fertilized (2PN)—Day 1 | 29/46 (63%)*a | 24/39 (61.5%)*b | 59/70 (84.2%) | |
| Abnormally fertilized | 3PN | 1/46 (2.1%) | 4/39 (10.2%) | 1/70 (1.4%) |
| 4PN | – | 1/39 (2.5%)*c | – | |
| Direct cleavage | 3/46 (6.5%) | 3/39 (7.6%) | – | |
| 2-cells stage—Day 2 | 19/29 (65.5%) | 17/24 (70.8%) | 47/59 (79.6%) | |
| 4–8 cells stage—Day 3 | 13/29 (44.8%)*d | 16/24 (66.6%) | 42/59 (71.1%) | |
| Blastocyst stage—Day 5 | 6/29 (20.6%) | 7/24 (29.1%) | 15/59 (25.4%) | |
| . | ST-OTO . | ST-Control . | ICSI-Control . | |
|---|---|---|---|---|
| Injected oocytes | 46 | 39 | 70 | |
| Normally fertilized (2PN)—Day 1 | 29/46 (63%)*a | 24/39 (61.5%)*b | 59/70 (84.2%) | |
| Abnormally fertilized | 3PN | 1/46 (2.1%) | 4/39 (10.2%) | 1/70 (1.4%) |
| 4PN | – | 1/39 (2.5%)*c | – | |
| Direct cleavage | 3/46 (6.5%) | 3/39 (7.6%) | – | |
| 2-cells stage—Day 2 | 19/29 (65.5%) | 17/24 (70.8%) | 47/59 (79.6%) | |
| 4–8 cells stage—Day 3 | 13/29 (44.8%)*d | 16/24 (66.6%) | 42/59 (71.1%) | |
| Blastocyst stage—Day 5 | 6/29 (20.6%) | 7/24 (29.1%) | 15/59 (25.4%) | |
OTO, ovarian tissue oocytes collected (in cold medium) from transgender men and matured in vitro; control oocytes, immature oocytes or mature oocytes (with aggregates of smooth endoplasmic reticulum) collected from ovarian stimulated women undergoing IVF; ST, spindle transfer; PN, pronuclei.
Fertilization was performed using ICSI with a single sperm cell. Blastocyst rates were calculated based on the number of normally fertilized zygotes. Percentages were compared using the chi-square test and P < 0.05 was considered significant. Asterisks represent a P value <0.05 between ST-OTO, Control-ST, and ICSI-Control oocytes.
P = 0.009.
P = 0.008.
P < 0.01.
P = 0.016.
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