| . | OTO-Cold . | OTO-Warm . | ICSI-Control . | |
|---|---|---|---|---|
| Injected oocytes | 47 | 48 | 70 | |
| Normally fertilized (2PN)—Day 1 | 19/47 (40.4%)*a | 24/48 (50.0%)*b | 59/70 (84.2%) | |
| Abnormally fertilized | 1PN | – | 2/48 (4.1%) | – |
| 3PN | 2/47 (4.2%) | 3/48 (6.2%)*c | 1/70 (1.4%) | |
| Direct cleavage | 1/47 (2.1%) | – | – | |
| 2-cells stage—Day 2 | 15/19 (78.9%) | 21/24 (87.5%) | 47/59 (79.6%) | |
| 4–8 cells stage—Day 3 | 7/19 (36.8%)*d | 14/24 (58.3%) | 42/59 (71.1%) | |
| Blastocyst stage—Day 5 | –*e | 1/24 (4.1%)*f | 15/59 (25.4%) | |
| Blastocyst stage—Day 6 | 1/19 (5.2%) | 1/24 (4.1%) | – | |
| . | OTO-Cold . | OTO-Warm . | ICSI-Control . | |
|---|---|---|---|---|
| Injected oocytes | 47 | 48 | 70 | |
| Normally fertilized (2PN)—Day 1 | 19/47 (40.4%)*a | 24/48 (50.0%)*b | 59/70 (84.2%) | |
| Abnormally fertilized | 1PN | – | 2/48 (4.1%) | – |
| 3PN | 2/47 (4.2%) | 3/48 (6.2%)*c | 1/70 (1.4%) | |
| Direct cleavage | 1/47 (2.1%) | – | – | |
| 2-cells stage—Day 2 | 15/19 (78.9%) | 21/24 (87.5%) | 47/59 (79.6%) | |
| 4–8 cells stage—Day 3 | 7/19 (36.8%)*d | 14/24 (58.3%) | 42/59 (71.1%) | |
| Blastocyst stage—Day 5 | –*e | 1/24 (4.1%)*f | 15/59 (25.4%) | |
| Blastocyst stage—Day 6 | 1/19 (5.2%) | 1/24 (4.1%) | – | |
OTO, ovarian tissue oocytes collected (in cold or warm medium) from transgender men and matured in vitro; control oocytes, immature oocytes or mature oocytes (with aggregates of smooth endoplasmic reticulum) collected from ovarian stimulated women undergoing IVF; PN, pronuclei.
Fertilization was performed using ICSI with a single sperm cell. Blastocyst rates were calculated based on the number of normally fertilized zygotes. Percentages were compared using the chi-square test and P < 0.05 was considered significant. Asterisks represent a P value <0.05 between respective groups and ICSI-Control oocytes.
P < 0.001.
P < 0.001.
P = 0.029.
P = 0.007.
P = 0.014.
P = 0.026.
| . | OTO-Cold . | OTO-Warm . | ICSI-Control . | |
|---|---|---|---|---|
| Injected oocytes | 47 | 48 | 70 | |
| Normally fertilized (2PN)—Day 1 | 19/47 (40.4%)*a | 24/48 (50.0%)*b | 59/70 (84.2%) | |
| Abnormally fertilized | 1PN | – | 2/48 (4.1%) | – |
| 3PN | 2/47 (4.2%) | 3/48 (6.2%)*c | 1/70 (1.4%) | |
| Direct cleavage | 1/47 (2.1%) | – | – | |
| 2-cells stage—Day 2 | 15/19 (78.9%) | 21/24 (87.5%) | 47/59 (79.6%) | |
| 4–8 cells stage—Day 3 | 7/19 (36.8%)*d | 14/24 (58.3%) | 42/59 (71.1%) | |
| Blastocyst stage—Day 5 | –*e | 1/24 (4.1%)*f | 15/59 (25.4%) | |
| Blastocyst stage—Day 6 | 1/19 (5.2%) | 1/24 (4.1%) | – | |
| . | OTO-Cold . | OTO-Warm . | ICSI-Control . | |
|---|---|---|---|---|
| Injected oocytes | 47 | 48 | 70 | |
| Normally fertilized (2PN)—Day 1 | 19/47 (40.4%)*a | 24/48 (50.0%)*b | 59/70 (84.2%) | |
| Abnormally fertilized | 1PN | – | 2/48 (4.1%) | – |
| 3PN | 2/47 (4.2%) | 3/48 (6.2%)*c | 1/70 (1.4%) | |
| Direct cleavage | 1/47 (2.1%) | – | – | |
| 2-cells stage—Day 2 | 15/19 (78.9%) | 21/24 (87.5%) | 47/59 (79.6%) | |
| 4–8 cells stage—Day 3 | 7/19 (36.8%)*d | 14/24 (58.3%) | 42/59 (71.1%) | |
| Blastocyst stage—Day 5 | –*e | 1/24 (4.1%)*f | 15/59 (25.4%) | |
| Blastocyst stage—Day 6 | 1/19 (5.2%) | 1/24 (4.1%) | – | |
OTO, ovarian tissue oocytes collected (in cold or warm medium) from transgender men and matured in vitro; control oocytes, immature oocytes or mature oocytes (with aggregates of smooth endoplasmic reticulum) collected from ovarian stimulated women undergoing IVF; PN, pronuclei.
Fertilization was performed using ICSI with a single sperm cell. Blastocyst rates were calculated based on the number of normally fertilized zygotes. Percentages were compared using the chi-square test and P < 0.05 was considered significant. Asterisks represent a P value <0.05 between respective groups and ICSI-Control oocytes.
P < 0.001.
P < 0.001.
P = 0.029.
P = 0.007.
P = 0.014.
P = 0.026.
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