Table 3 Open in new tab FA compositions...

Materials

Protein content (%)

C16:0 (%)

C18:0 (%)

C18:1 (%)

C18:2 (%)

C18:3 (%)

C20:1 (%)

23.92 ± 1.46

4.86 ± 0.15

2.60 ± 0.50

57.81 ± 1.65

17.84 ± 1.07

8.41 ± 0.77

2.71 ± 0.59

23Ri-L1-7

23.25 ± 0.62

4.85 ± 0.11

2.31 ± 0.13

61.94 ± 0.43**

17.14 ± 0.22

8.86 ± 0.94

2.90 ± 0.14

23Ri-L2-19

22.71 ± 0.29

4.54 ± 0.10**

2.14 ± 0.11

67.85 ± 0.40**

14.69 ± 0.09**

8.09 ± 0.58

2.03 ± 0.75

23Ri-L3-27

24.62 ± 0.48

4.54 ± 0.10**

2.17 ± 0.14

67.09 ± 1.31**

15.13 ± 0.21**

7.91 ± 0.42

2.26 ± 1.02

23Ri-L4-35

22.97 ± 0.24

4.76 ± 0.11

2.22 ± 0.06

62.45 ± 0.52**

16.82 ± 0.65

8.59 ± 0.46

2.24 ± 0.80

23Ri-L5-46

22.92 ± 0.41

4.67 ± 0.13

2.43 ± 0.20

62.16 ± 0.63**

17.47 ± 0.59

8.45 ± 0.23

3.17 ± 0.48

22Ri-L1-53

23.14 ± 0.43

4.67 ± 0.09

2.39 ± 0.15

62.11 ± 0.88**

16.95 ± 0.33

8.37 ± 0.44

2.66 ± 0.39

22Ri-L2-60

22.48 ± 0.80

4.56 ± 0.22

2.24 ± 0.11

67.99 ± 1.20**

14.39 ± 1.06**

8.01 ± 0.56

2.23 ± 1.15

22Ri-L3-66

20.49 ± 0.93*

4.51 ± 0.22

2.08 ± 0.13

67.47 ± 1.40**

15.00 ± 0.41**

8.48 ± 0.68

2.11 ± 1.36

22Ri-L4-72

22.71 ± 0.39

4.67 ± 0.12

2.23 ± 0.17

64.01 ± 1.14**

14.21 ± 0.64**

7.92 ± 0.82

2.41 ± 0.73

22Ri-L5-77

22.22 ± 0.46

4.67 ± 0.09

2.37 ± 0.14

65.39 ± 0.71**

14.35 ± 0.61**

8.52 ± 0.51

2.98 ± 1.25

22Ri-L6-84

21.80 ± 0.94

4.78 ± 0.15

2.30 ± 0.19

64.24 ± 0.43**

14.68 ± 0.71**

8.29 ± 0.14

2.67 ± 1.12

22.98 ± 0.44

4.85 ± 0.22

2.16 ± 0.08

61.60 ± 0.58

17.27 ± 0.46

8.42 ± 0.46

2.36 ± 0.99

23Ri-L1-7-5

21.97 ± 1.26

4.60 ± 0.19

2.30 ± 0.22

68.91 ± 0.71**

14.54 ± 1.07**

7.94 ± 1.07

2.45 ± 0.56

23Ri-L2-19-12

21.18 ± 0.72

4.47 ± 0.07

2.20 ± 0.10

69.69 ± 0.57**

13.76 ± 0.43**

8.36 ± 0.43

2.23 ± 0.23

23Ri-L3-27-17

22.74 ± 0.59

4.46 ± 0.10

2.32 ± 0.14

66.62 ± 0.70**

14.6 ± 0.83**

8.04 ± 0.83

3.35 ± 0.40

23Ri-L4-35-24

22.03 ± 1.29

4.48 ± 0.14

2.12 ± 0.13

69.12 ± 0.75**

13.85 ± 0.75**

8.47 ± 0.75

1.81 ± 0.32

23Ri-L5-46-31

21.61 ± 0.89

4.65 ± 0.17

2.33 ± 0.25

66.31 ± 1.24**

15.23 ± 0.38**

8.35 ± 0.38

2.24 ± 0.14

22Ri-L1-53-33

21.59 ± 0.97

4.54 ± 0.09**

2.30 ± 0.02

66.77 ± 0.48**

14.12 ± 0.51**

8.18 ± 0.51

3.62 ± 0.27

22Ri-L2-60-44

21.29 ± 0.51

4.56 ± 0.07

2.29 ± 0.03**

66.86 ± 0.57**

13.92 ± 1.16

8.12 ± 1.16

3.42 ± 0.29

22Ri-L3-66-52

20.54 ± 0.49*

4.39 ± 0.04**

2.27 ± 0.14

64.82 ± 0.81**

14.62 ± 0.91**

8.31 ± 0.91

3.45 ± 0.50

22Ri-L4-72-65

22.22 ± 0.58

4.56 ± 0.04

2.48 ± 0.22

65.23 ± 1.93**

13.43 ± 0.64**

8.24 ± 0.64

3.05 ± 0.14

22Ri-L5-77-72

21.38 ± 0.76

4.51 ± 0.12

2.25 ± 0.15

66.29 ± 0.81**

13.95 ± 0.86**

8.39 ± 0.86

2.63 ± 0.43

22Ri-L6-84-81

22.68 ± 0.83

4.53 ± 0.16

2.32 ± 0.16

65.27 ± 0.64**

14.66 ± 1.02**

8.43 ± 1.02

3.38 ± 0.52

26.62 ± 1.09

3.96 ± 0.09

1.82 ± 0.04

55.83 ± 0.81

19.37 ± 0.63

9.89 ± 0.17

1.16 ± 0.24

MT1

25.45 ± 0.95

3.18 ± 0.12

1.83 ± 0.1

58.3 ± 0.45**

16.79 ± 0.61**

8.06 ± 0.41*

1.49 ± 0.38

MT2

25.14 ± 1.20

3.55 ± 0.17

1.96 ± 0.03

59.17 ± 1.2**

15.67 ± 0.18**

7.91 ± 0.24**

1.31 ± 0.08

MT3

26.15 ± 0.70

3.66 ± 0.04

1.78 ± 0.09

56.75 ± 1.24**

18.76 ± 0.37

7.83 ± 0.52**

1.1 ± 0.02

MT4

25.84 ± 1.23

3.49 ± 0.04

1.8 ± 0.09

58.96 ± 0.98**

18.22 ± 0.6**

6.99 ± 0.21*

1.12 ± 0.05

MT5

24.12 ± 0.33*

3.45 ± 0.06

1.47 ± 0.15

56.58 ± 0.46*

17.6 ± 0.45**

8.09 ± 0.42*

1.64 ± 0.14

MT6

26.33 ± 0.94

3.36 ± 0.05

1.27 ± 0.11

58.22 ± 1.34**

16.98 ± 0.19**

8.22 ± 0.16*

1.61 ± 0.05

25.4 ± 1.12

3.59 ± 0.12

1.8 ± 0.07

57.87 ± 0.52

18.1 ± 0.16

8.91 ± 0.24

1.84 ± 0.17

MT1

24.52 ± 0.98

3.62 ± 0.38

1.57 ± 0.31

60.1 ± 0.35*

17.24 ± 0.23

7.48 ± 0.23*

1.57 ± 0.32

MT2

24.17 ± 0.06

3.73 ± 0.33

1.34 ± 0.11

60.54 ± 0.22*

17.2 ± 0.21*

6.23 ± 0.43*

1.94 ± 0.2

MT3

22.44 ± 0.56**

3.87 ± 0.49

1.57 ± 0.01

62.28 ± 0.32**

16.48 ± 0.15**

6.1 ± 0.3**

2.06 ± 0.27

MT4

24.98 ± 1.23

3.53 ± 0.28

1.66 ± 0.36

62.2 ± 0.3**

16.32 ± 0.12**

6.48 ± 0.34*

1.74 ± 0.34

MT5

25.07 ± 0.78

3.29 ± 0.19

1.47 ± 0.25

60.7 ± 0.35*

15.81 ± 0.51*

5.99 ± 0.48**

1.88 ± 0.18

MT6

25.17 ± 0.49

3.82 ± 0.12

1.76 ± 0.33

61.75 ± 0.24*

17.79 ± 0.14

6.47 ± 0.28*

1.55 ± 0.24

Materials

Protein content (%)

C16:0 (%)

C18:0 (%)

C18:1 (%)

C18:2 (%)

C18:3 (%)

C20:1 (%)

23.92 ± 1.46

4.86 ± 0.15

2.60 ± 0.50

57.81 ± 1.65

17.84 ± 1.07

8.41 ± 0.77

2.71 ± 0.59

23Ri-L1-7

23.25 ± 0.62

4.85 ± 0.11

2.31 ± 0.13

61.94 ± 0.43**

17.14 ± 0.22

8.86 ± 0.94

2.90 ± 0.14

23Ri-L2-19

22.71 ± 0.29

4.54 ± 0.10**

2.14 ± 0.11

67.85 ± 0.40**

14.69 ± 0.09**

8.09 ± 0.58

2.03 ± 0.75

23Ri-L3-27

24.62 ± 0.48

4.54 ± 0.10**

2.17 ± 0.14

67.09 ± 1.31**

15.13 ± 0.21**

7.91 ± 0.42

2.26 ± 1.02

23Ri-L4-35

22.97 ± 0.24

4.76 ± 0.11

2.22 ± 0.06

62.45 ± 0.52**

16.82 ± 0.65

8.59 ± 0.46

2.24 ± 0.80

23Ri-L5-46

22.92 ± 0.41

4.67 ± 0.13

2.43 ± 0.20

62.16 ± 0.63**

17.47 ± 0.59

8.45 ± 0.23

3.17 ± 0.48

22Ri-L1-53

23.14 ± 0.43

4.67 ± 0.09

2.39 ± 0.15

62.11 ± 0.88**

16.95 ± 0.33

8.37 ± 0.44

2.66 ± 0.39

22Ri-L2-60

22.48 ± 0.80

4.56 ± 0.22

2.24 ± 0.11

67.99 ± 1.20**

14.39 ± 1.06**

8.01 ± 0.56

2.23 ± 1.15

22Ri-L3-66

20.49 ± 0.93*

4.51 ± 0.22

2.08 ± 0.13

67.47 ± 1.40**

15.00 ± 0.41**

8.48 ± 0.68

2.11 ± 1.36

22Ri-L4-72

22.71 ± 0.39

4.67 ± 0.12

2.23 ± 0.17

64.01 ± 1.14**

14.21 ± 0.64**

7.92 ± 0.82

2.41 ± 0.73

22Ri-L5-77

22.22 ± 0.46

4.67 ± 0.09

2.37 ± 0.14

65.39 ± 0.71**

14.35 ± 0.61**

8.52 ± 0.51

2.98 ± 1.25

22Ri-L6-84

21.80 ± 0.94

4.78 ± 0.15

2.30 ± 0.19

64.24 ± 0.43**

14.68 ± 0.71**

8.29 ± 0.14

2.67 ± 1.12

22.98 ± 0.44

4.85 ± 0.22

2.16 ± 0.08

61.60 ± 0.58

17.27 ± 0.46

8.42 ± 0.46

2.36 ± 0.99

23Ri-L1-7-5

21.97 ± 1.26

4.60 ± 0.19

2.30 ± 0.22

68.91 ± 0.71**

14.54 ± 1.07**

7.94 ± 1.07

2.45 ± 0.56

23Ri-L2-19-12

21.18 ± 0.72

4.47 ± 0.07

2.20 ± 0.10

69.69 ± 0.57**

13.76 ± 0.43**

8.36 ± 0.43

2.23 ± 0.23

23Ri-L3-27-17

22.74 ± 0.59

4.46 ± 0.10

2.32 ± 0.14

66.62 ± 0.70**

14.6 ± 0.83**

8.04 ± 0.83

3.35 ± 0.40

23Ri-L4-35-24

22.03 ± 1.29

4.48 ± 0.14

2.12 ± 0.13

69.12 ± 0.75**

13.85 ± 0.75**

8.47 ± 0.75

1.81 ± 0.32

23Ri-L5-46-31

21.61 ± 0.89

4.65 ± 0.17

2.33 ± 0.25

66.31 ± 1.24**

15.23 ± 0.38**

8.35 ± 0.38

2.24 ± 0.14

22Ri-L1-53-33

21.59 ± 0.97

4.54 ± 0.09**

2.30 ± 0.02

66.77 ± 0.48**

14.12 ± 0.51**

8.18 ± 0.51

3.62 ± 0.27

22Ri-L2-60-44

21.29 ± 0.51

4.56 ± 0.07

2.29 ± 0.03**

66.86 ± 0.57**

13.92 ± 1.16

8.12 ± 1.16

3.42 ± 0.29

22Ri-L3-66-52

20.54 ± 0.49*

4.39 ± 0.04**

2.27 ± 0.14

64.82 ± 0.81**

14.62 ± 0.91**

8.31 ± 0.91

3.45 ± 0.50

22Ri-L4-72-65

22.22 ± 0.58

4.56 ± 0.04

2.48 ± 0.22

65.23 ± 1.93**

13.43 ± 0.64**

8.24 ± 0.64

3.05 ± 0.14

22Ri-L5-77-72

21.38 ± 0.76

4.51 ± 0.12

2.25 ± 0.15

66.29 ± 0.81**

13.95 ± 0.86**

8.39 ± 0.86

2.63 ± 0.43

22Ri-L6-84-81

22.68 ± 0.83

4.53 ± 0.16

2.32 ± 0.16

65.27 ± 0.64**

14.66 ± 1.02**

8.43 ± 1.02

3.38 ± 0.52

26.62 ± 1.09

3.96 ± 0.09

1.82 ± 0.04

55.83 ± 0.81

19.37 ± 0.63

9.89 ± 0.17

1.16 ± 0.24

MT1

25.45 ± 0.95

3.18 ± 0.12

1.83 ± 0.1

58.3 ± 0.45**

16.79 ± 0.61**

8.06 ± 0.41*

1.49 ± 0.38

MT2

25.14 ± 1.20

3.55 ± 0.17

1.96 ± 0.03

59.17 ± 1.2**

15.67 ± 0.18**

7.91 ± 0.24**

1.31 ± 0.08

MT3

26.15 ± 0.70

3.66 ± 0.04

1.78 ± 0.09

56.75 ± 1.24**

18.76 ± 0.37

7.83 ± 0.52**

1.1 ± 0.02

MT4

25.84 ± 1.23

3.49 ± 0.04

1.8 ± 0.09

58.96 ± 0.98**

18.22 ± 0.6**

6.99 ± 0.21*

1.12 ± 0.05

MT5

24.12 ± 0.33*

3.45 ± 0.06

1.47 ± 0.15

56.58 ± 0.46*

17.6 ± 0.45**

8.09 ± 0.42*

1.64 ± 0.14

MT6

26.33 ± 0.94

3.36 ± 0.05

1.27 ± 0.11

58.22 ± 1.34**

16.98 ± 0.19**

8.22 ± 0.16*

1.61 ± 0.05

25.4 ± 1.12

3.59 ± 0.12

1.8 ± 0.07

57.87 ± 0.52

18.1 ± 0.16

8.91 ± 0.24

1.84 ± 0.17

MT1

24.52 ± 0.98

3.62 ± 0.38

1.57 ± 0.31

60.1 ± 0.35*

17.24 ± 0.23

7.48 ± 0.23*

1.57 ± 0.32

MT2

24.17 ± 0.06

3.73 ± 0.33

1.34 ± 0.11

60.54 ± 0.22*

17.2 ± 0.21*

6.23 ± 0.43*

1.94 ± 0.2

MT3

22.44 ± 0.56**

3.87 ± 0.49

1.57 ± 0.01

62.28 ± 0.32**

16.48 ± 0.15**

6.1 ± 0.3**

2.06 ± 0.27

MT4

24.98 ± 1.23

3.53 ± 0.28

1.66 ± 0.36

62.2 ± 0.3**

16.32 ± 0.12**

6.48 ± 0.34*

1.74 ± 0.34

MT5

25.07 ± 0.78

3.29 ± 0.19

1.47 ± 0.25

60.7 ± 0.35*

15.81 ± 0.51*

5.99 ± 0.48**

1.88 ± 0.18

MT6

25.17 ± 0.49

3.82 ± 0.12

1.76 ± 0.33

61.75 ± 0.24*

17.79 ± 0.14

6.47 ± 0.28*

1.55 ± 0.24

Table 3

Open in new tab

FA compositions and protein content in mature seeds of gene-silenced and loss-of-function mutant lines

	Materials	Protein content (%)	C16:0 (%)	C18:0 (%)	C18:1 (%)	C18:2 (%)	C18:3 (%)	C20:1 (%)
T1	WT	23.92 ± 1.46	4.86 ± 0.15	2.60 ± 0.50	57.81 ± 1.65	17.84 ± 1.07	8.41 ± 0.77	2.71 ± 0.59
	23Ri-L1-7	23.25 ± 0.62	4.85 ± 0.11	2.31 ± 0.13	61.94 ± 0.43**	17.14 ± 0.22	8.86 ± 0.94	2.90 ± 0.14
	23Ri-L2-19	22.71 ± 0.29	4.54 ± 0.10**	2.14 ± 0.11	67.85 ± 0.40**	14.69 ± 0.09**	8.09 ± 0.58	2.03 ± 0.75
	23Ri-L3-27	24.62 ± 0.48	4.54 ± 0.10**	2.17 ± 0.14	67.09 ± 1.31**	15.13 ± 0.21**	7.91 ± 0.42	2.26 ± 1.02
	23Ri-L4-35	22.97 ± 0.24	4.76 ± 0.11	2.22 ± 0.06	62.45 ± 0.52**	16.82 ± 0.65	8.59 ± 0.46	2.24 ± 0.80
	23Ri-L5-46	22.92 ± 0.41	4.67 ± 0.13	2.43 ± 0.20	62.16 ± 0.63**	17.47 ± 0.59	8.45 ± 0.23	3.17 ± 0.48
	22Ri-L1-53	23.14 ± 0.43	4.67 ± 0.09	2.39 ± 0.15	62.11 ± 0.88**	16.95 ± 0.33	8.37 ± 0.44	2.66 ± 0.39
	22Ri-L2-60	22.48 ± 0.80	4.56 ± 0.22	2.24 ± 0.11	67.99 ± 1.20**	14.39 ± 1.06**	8.01 ± 0.56	2.23 ± 1.15
	22Ri-L3-66	20.49 ± 0.93*	4.51 ± 0.22	2.08 ± 0.13	67.47 ± 1.40**	15.00 ± 0.41**	8.48 ± 0.68	2.11 ± 1.36
	22Ri-L4-72	22.71 ± 0.39	4.67 ± 0.12	2.23 ± 0.17	64.01 ± 1.14**	14.21 ± 0.64**	7.92 ± 0.82	2.41 ± 0.73
	22Ri-L5-77	22.22 ± 0.46	4.67 ± 0.09	2.37 ± 0.14	65.39 ± 0.71**	14.35 ± 0.61**	8.52 ± 0.51	2.98 ± 1.25
	22Ri-L6-84	21.80 ± 0.94	4.78 ± 0.15	2.30 ± 0.19	64.24 ± 0.43**	14.68 ± 0.71**	8.29 ± 0.14	2.67 ± 1.12
T2	WT	22.98 ± 0.44	4.85 ± 0.22	2.16 ± 0.08	61.60 ± 0.58	17.27 ± 0.46	8.42 ± 0.46	2.36 ± 0.99
	23Ri-L1-7-5	21.97 ± 1.26	4.60 ± 0.19	2.30 ± 0.22	68.91 ± 0.71**	14.54 ± 1.07**	7.94 ± 1.07	2.45 ± 0.56
	23Ri-L2-19-12	21.18 ± 0.72	4.47 ± 0.07	2.20 ± 0.10	69.69 ± 0.57**	13.76 ± 0.43**	8.36 ± 0.43	2.23 ± 0.23
	23Ri-L3-27-17	22.74 ± 0.59	4.46 ± 0.10	2.32 ± 0.14	66.62 ± 0.70**	14.6 ± 0.83**	8.04 ± 0.83	3.35 ± 0.40
	23Ri-L4-35-24	22.03 ± 1.29	4.48 ± 0.14	2.12 ± 0.13	69.12 ± 0.75**	13.85 ± 0.75**	8.47 ± 0.75	1.81 ± 0.32
	23Ri-L5-46-31	21.61 ± 0.89	4.65 ± 0.17	2.33 ± 0.25	66.31 ± 1.24**	15.23 ± 0.38**	8.35 ± 0.38	2.24 ± 0.14
	22Ri-L1-53-33	21.59 ± 0.97	4.54 ± 0.09**	2.30 ± 0.02	66.77 ± 0.48**	14.12 ± 0.51**	8.18 ± 0.51	3.62 ± 0.27
	22Ri-L2-60-44	21.29 ± 0.51	4.56 ± 0.07	2.29 ± 0.03**	66.86 ± 0.57**	13.92 ± 1.16	8.12 ± 1.16	3.42 ± 0.29
	22Ri-L3-66-52	20.54 ± 0.49*	4.39 ± 0.04**	2.27 ± 0.14	64.82 ± 0.81**	14.62 ± 0.91**	8.31 ± 0.91	3.45 ± 0.50
	22Ri-L4-72-65	22.22 ± 0.58	4.56 ± 0.04	2.48 ± 0.22	65.23 ± 1.93**	13.43 ± 0.64**	8.24 ± 0.64	3.05 ± 0.14
	22Ri-L5-77-72	21.38 ± 0.76	4.51 ± 0.12	2.25 ± 0.15	66.29 ± 0.81**	13.95 ± 0.86**	8.39 ± 0.86	2.63 ± 0.43
	22Ri-L6-84-81	22.68 ± 0.83	4.53 ± 0.16	2.32 ± 0.16	65.27 ± 0.64**	14.66 ± 1.02**	8.43 ± 1.02	3.38 ± 0.52
T2	WT	26.62 ± 1.09	3.96 ± 0.09	1.82 ± 0.04	55.83 ± 0.81	19.37 ± 0.63	9.89 ± 0.17	1.16 ± 0.24
	MT1	25.45 ± 0.95	3.18 ± 0.12	1.83 ± 0.1	58.3 ± 0.45**	16.79 ± 0.61**	8.06 ± 0.41*	1.49 ± 0.38
	MT2	25.14 ± 1.20	3.55 ± 0.17	1.96 ± 0.03	59.17 ± 1.2**	15.67 ± 0.18**	7.91 ± 0.24**	1.31 ± 0.08
	MT3	26.15 ± 0.70	3.66 ± 0.04	1.78 ± 0.09	56.75 ± 1.24**	18.76 ± 0.37	7.83 ± 0.52**	1.1 ± 0.02
	MT4	25.84 ± 1.23	3.49 ± 0.04	1.8 ± 0.09	58.96 ± 0.98**	18.22 ± 0.6**	6.99 ± 0.21*	1.12 ± 0.05
	MT5	24.12 ± 0.33*	3.45 ± 0.06	1.47 ± 0.15	56.58 ± 0.46*	17.6 ± 0.45**	8.09 ± 0.42*	1.64 ± 0.14
	MT6	26.33 ± 0.94	3.36 ± 0.05	1.27 ± 0.11	58.22 ± 1.34**	16.98 ± 0.19**	8.22 ± 0.16*	1.61 ± 0.05
T3	WT	25.4 ± 1.12	3.59 ± 0.12	1.8 ± 0.07	57.87 ± 0.52	18.1 ± 0.16	8.91 ± 0.24	1.84 ± 0.17
	MT1	24.52 ± 0.98	3.62 ± 0.38	1.57 ± 0.31	60.1 ± 0.35*	17.24 ± 0.23	7.48 ± 0.23*	1.57 ± 0.32
	MT2	24.17 ± 0.06	3.73 ± 0.33	1.34 ± 0.11	60.54 ± 0.22*	17.2 ± 0.21*	6.23 ± 0.43*	1.94 ± 0.2
	MT3	22.44 ± 0.56**	3.87 ± 0.49	1.57 ± 0.01	62.28 ± 0.32**	16.48 ± 0.15**	6.1 ± 0.3**	2.06 ± 0.27
	MT4	24.98 ± 1.23	3.53 ± 0.28	1.66 ± 0.36	62.2 ± 0.3**	16.32 ± 0.12**	6.48 ± 0.34*	1.74 ± 0.34
	MT5	25.07 ± 0.78	3.29 ± 0.19	1.47 ± 0.25	60.7 ± 0.35*	15.81 ± 0.51*	5.99 ± 0.48**	1.88 ± 0.18
	MT6	25.17 ± 0.49	3.82 ± 0.12	1.76 ± 0.33	61.75 ± 0.24*	17.79 ± 0.14	6.47 ± 0.28*	1.55 ± 0.24

	Materials	Protein content (%)	C16:0 (%)	C18:0 (%)	C18:1 (%)	C18:2 (%)	C18:3 (%)	C20:1 (%)
T1	WT	23.92 ± 1.46	4.86 ± 0.15	2.60 ± 0.50	57.81 ± 1.65	17.84 ± 1.07	8.41 ± 0.77	2.71 ± 0.59
	23Ri-L1-7	23.25 ± 0.62	4.85 ± 0.11	2.31 ± 0.13	61.94 ± 0.43**	17.14 ± 0.22	8.86 ± 0.94	2.90 ± 0.14
	23Ri-L2-19	22.71 ± 0.29	4.54 ± 0.10**	2.14 ± 0.11	67.85 ± 0.40**	14.69 ± 0.09**	8.09 ± 0.58	2.03 ± 0.75
	23Ri-L3-27	24.62 ± 0.48	4.54 ± 0.10**	2.17 ± 0.14	67.09 ± 1.31**	15.13 ± 0.21**	7.91 ± 0.42	2.26 ± 1.02
	23Ri-L4-35	22.97 ± 0.24	4.76 ± 0.11	2.22 ± 0.06	62.45 ± 0.52**	16.82 ± 0.65	8.59 ± 0.46	2.24 ± 0.80
	23Ri-L5-46	22.92 ± 0.41	4.67 ± 0.13	2.43 ± 0.20	62.16 ± 0.63**	17.47 ± 0.59	8.45 ± 0.23	3.17 ± 0.48
	22Ri-L1-53	23.14 ± 0.43	4.67 ± 0.09	2.39 ± 0.15	62.11 ± 0.88**	16.95 ± 0.33	8.37 ± 0.44	2.66 ± 0.39
	22Ri-L2-60	22.48 ± 0.80	4.56 ± 0.22	2.24 ± 0.11	67.99 ± 1.20**	14.39 ± 1.06**	8.01 ± 0.56	2.23 ± 1.15
	22Ri-L3-66	20.49 ± 0.93*	4.51 ± 0.22	2.08 ± 0.13	67.47 ± 1.40**	15.00 ± 0.41**	8.48 ± 0.68	2.11 ± 1.36
	22Ri-L4-72	22.71 ± 0.39	4.67 ± 0.12	2.23 ± 0.17	64.01 ± 1.14**	14.21 ± 0.64**	7.92 ± 0.82	2.41 ± 0.73
	22Ri-L5-77	22.22 ± 0.46	4.67 ± 0.09	2.37 ± 0.14	65.39 ± 0.71**	14.35 ± 0.61**	8.52 ± 0.51	2.98 ± 1.25
	22Ri-L6-84	21.80 ± 0.94	4.78 ± 0.15	2.30 ± 0.19	64.24 ± 0.43**	14.68 ± 0.71**	8.29 ± 0.14	2.67 ± 1.12
T2	WT	22.98 ± 0.44	4.85 ± 0.22	2.16 ± 0.08	61.60 ± 0.58	17.27 ± 0.46	8.42 ± 0.46	2.36 ± 0.99
	23Ri-L1-7-5	21.97 ± 1.26	4.60 ± 0.19	2.30 ± 0.22	68.91 ± 0.71**	14.54 ± 1.07**	7.94 ± 1.07	2.45 ± 0.56
	23Ri-L2-19-12	21.18 ± 0.72	4.47 ± 0.07	2.20 ± 0.10	69.69 ± 0.57**	13.76 ± 0.43**	8.36 ± 0.43	2.23 ± 0.23
	23Ri-L3-27-17	22.74 ± 0.59	4.46 ± 0.10	2.32 ± 0.14	66.62 ± 0.70**	14.6 ± 0.83**	8.04 ± 0.83	3.35 ± 0.40
	23Ri-L4-35-24	22.03 ± 1.29	4.48 ± 0.14	2.12 ± 0.13	69.12 ± 0.75**	13.85 ± 0.75**	8.47 ± 0.75	1.81 ± 0.32
	23Ri-L5-46-31	21.61 ± 0.89	4.65 ± 0.17	2.33 ± 0.25	66.31 ± 1.24**	15.23 ± 0.38**	8.35 ± 0.38	2.24 ± 0.14
	22Ri-L1-53-33	21.59 ± 0.97	4.54 ± 0.09**	2.30 ± 0.02	66.77 ± 0.48**	14.12 ± 0.51**	8.18 ± 0.51	3.62 ± 0.27
	22Ri-L2-60-44	21.29 ± 0.51	4.56 ± 0.07	2.29 ± 0.03**	66.86 ± 0.57**	13.92 ± 1.16	8.12 ± 1.16	3.42 ± 0.29
	22Ri-L3-66-52	20.54 ± 0.49*	4.39 ± 0.04**	2.27 ± 0.14	64.82 ± 0.81**	14.62 ± 0.91**	8.31 ± 0.91	3.45 ± 0.50
	22Ri-L4-72-65	22.22 ± 0.58	4.56 ± 0.04	2.48 ± 0.22	65.23 ± 1.93**	13.43 ± 0.64**	8.24 ± 0.64	3.05 ± 0.14
	22Ri-L5-77-72	21.38 ± 0.76	4.51 ± 0.12	2.25 ± 0.15	66.29 ± 0.81**	13.95 ± 0.86**	8.39 ± 0.86	2.63 ± 0.43
	22Ri-L6-84-81	22.68 ± 0.83	4.53 ± 0.16	2.32 ± 0.16	65.27 ± 0.64**	14.66 ± 1.02**	8.43 ± 1.02	3.38 ± 0.52
T2	WT	26.62 ± 1.09	3.96 ± 0.09	1.82 ± 0.04	55.83 ± 0.81	19.37 ± 0.63	9.89 ± 0.17	1.16 ± 0.24
	MT1	25.45 ± 0.95	3.18 ± 0.12	1.83 ± 0.1	58.3 ± 0.45**	16.79 ± 0.61**	8.06 ± 0.41*	1.49 ± 0.38
	MT2	25.14 ± 1.20	3.55 ± 0.17	1.96 ± 0.03	59.17 ± 1.2**	15.67 ± 0.18**	7.91 ± 0.24**	1.31 ± 0.08
	MT3	26.15 ± 0.70	3.66 ± 0.04	1.78 ± 0.09	56.75 ± 1.24**	18.76 ± 0.37	7.83 ± 0.52**	1.1 ± 0.02
	MT4	25.84 ± 1.23	3.49 ± 0.04	1.8 ± 0.09	58.96 ± 0.98**	18.22 ± 0.6**	6.99 ± 0.21*	1.12 ± 0.05
	MT5	24.12 ± 0.33*	3.45 ± 0.06	1.47 ± 0.15	56.58 ± 0.46*	17.6 ± 0.45**	8.09 ± 0.42*	1.64 ± 0.14
	MT6	26.33 ± 0.94	3.36 ± 0.05	1.27 ± 0.11	58.22 ± 1.34**	16.98 ± 0.19**	8.22 ± 0.16*	1.61 ± 0.05
T3	WT	25.4 ± 1.12	3.59 ± 0.12	1.8 ± 0.07	57.87 ± 0.52	18.1 ± 0.16	8.91 ± 0.24	1.84 ± 0.17
	MT1	24.52 ± 0.98	3.62 ± 0.38	1.57 ± 0.31	60.1 ± 0.35*	17.24 ± 0.23	7.48 ± 0.23*	1.57 ± 0.32
	MT2	24.17 ± 0.06	3.73 ± 0.33	1.34 ± 0.11	60.54 ± 0.22*	17.2 ± 0.21*	6.23 ± 0.43*	1.94 ± 0.2
	MT3	22.44 ± 0.56**	3.87 ± 0.49	1.57 ± 0.01	62.28 ± 0.32**	16.48 ± 0.15**	6.1 ± 0.3**	2.06 ± 0.27
	MT4	24.98 ± 1.23	3.53 ± 0.28	1.66 ± 0.36	62.2 ± 0.3**	16.32 ± 0.12**	6.48 ± 0.34*	1.74 ± 0.34
	MT5	25.07 ± 0.78	3.29 ± 0.19	1.47 ± 0.25	60.7 ± 0.35*	15.81 ± 0.51*	5.99 ± 0.48**	1.88 ± 0.18
	MT6	25.17 ± 0.49	3.82 ± 0.12	1.76 ± 0.33	61.75 ± 0.24*	17.79 ± 0.14	6.47 ± 0.28*	1.55 ± 0.24

23Ri and 22Ri, gene-silenced lines (RNAi lines); MT, the independent lines of Cas9-free loss-of-function mutants lines (bnacipk9 lines); WT, wild-type plant lines (Westar). Six independent lines were separately selected from the RNAi and bnacipk9 lines. The FA composition and protein content were measured using gas chromatography and the NIRS-System, respectively. Data are represented as the means ± SE corresponding to 5–7 inbred lines in each independent line. Asterisks denote significant differences (* P = 0.05 and ** P = 0.01) between the RNAi or bnacipk9 lines and WT plants based on the analysis of variance by the least-significant difference test.

This Feature Is Available To Subscribers Only