Table 1

The editing formats and frequencies of the two SgRNAs in rapeseed T0 plants

GenotypeEditing format
InsertionMutationChimeraDeletion
Sg1-AABB7A 3C 2T 1G2A-T 1C-G2I 1M 6Vm>3 bp 1
Sg2-AABB9A 3C 4T 1G4A-T 3C-G3I 3M 6Vm≤3 bp 1; >3 bp 1
Sg1-CC8A 4C 4T 3G2A-T 1C-G3I 3T 7Vm≤3 bp 1; >3 bp 1
Sg2-DD6A 3C 4T 1G2A-T 2C-G2I 2M 6Vm≤3 bp 1; >3 bp 1
GenotypeEditing format
InsertionMutationChimeraDeletion
Sg1-AABB7A 3C 2T 1G2A-T 1C-G2I 1M 6Vm>3 bp 1
Sg2-AABB9A 3C 4T 1G4A-T 3C-G3I 3M 6Vm≤3 bp 1; >3 bp 1
Sg1-CC8A 4C 4T 3G2A-T 1C-G3I 3T 7Vm≤3 bp 1; >3 bp 1
Sg2-DD6A 3C 4T 1G2A-T 2C-G2I 2M 6Vm≤3 bp 1; >3 bp 1

Vm, multiple mutation; I, SNP insertion; M, SNP mutation. AA, BB, CC, and DD indicate the four BnaCIPK9 alleles.

Table 1

The editing formats and frequencies of the two SgRNAs in rapeseed T0 plants

GenotypeEditing format
InsertionMutationChimeraDeletion
Sg1-AABB7A 3C 2T 1G2A-T 1C-G2I 1M 6Vm>3 bp 1
Sg2-AABB9A 3C 4T 1G4A-T 3C-G3I 3M 6Vm≤3 bp 1; >3 bp 1
Sg1-CC8A 4C 4T 3G2A-T 1C-G3I 3T 7Vm≤3 bp 1; >3 bp 1
Sg2-DD6A 3C 4T 1G2A-T 2C-G2I 2M 6Vm≤3 bp 1; >3 bp 1
GenotypeEditing format
InsertionMutationChimeraDeletion
Sg1-AABB7A 3C 2T 1G2A-T 1C-G2I 1M 6Vm>3 bp 1
Sg2-AABB9A 3C 4T 1G4A-T 3C-G3I 3M 6Vm≤3 bp 1; >3 bp 1
Sg1-CC8A 4C 4T 3G2A-T 1C-G3I 3T 7Vm≤3 bp 1; >3 bp 1
Sg2-DD6A 3C 4T 1G2A-T 2C-G2I 2M 6Vm≤3 bp 1; >3 bp 1

Vm, multiple mutation; I, SNP insertion; M, SNP mutation. AA, BB, CC, and DD indicate the four BnaCIPK9 alleles.

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