Apparent detachment rates (koff) and maximum number of replicated nucleotides (Nt) measured in strand displacement bulk assays. ΔGint/M are the minimum values of the free-parameters yielded by least squared error fits of strand displacement model to pause-free velocity data. K(0) and d are the values of free-parameters of Eq. 1 obtained upon fitting the tension dependent average residence times in pause state data (Tp(f)) with the least mean squared error. Tp(f), average residence times at pause state at the indicated tensions (superscript). In all cases, uncertainties are shown as standard errors
| . | koff (s−1) (bulk) . | Nt (bulk) . | ΔGint(kBT)/M . | K(0) . | d(nm) . | Tp(f) (s nt−1) . |
|---|---|---|---|---|---|---|
| Polγ | (6.26 ± 0.05) × 10−3 | 37 ± 15 | 0.90 ± 0.10 /1 | 24 ± 3 | 1.4 ± 0.4 | 3.10 ± 0.500pN |
| Polγ mtSSB | (8.21 ± 0.14) × 10−3 | 108 ± 42 | 1.40 ± 0.04 /1 | 10 ± 3 | 1.2 ± 0.3 | 1.23 ± 0.420pN |
| Polγ EcoSSB | (3.28 ± 0.04) × 10−3 | ≥150 | 1.38 ± 0.11 /1 | N.A. | N.A. | 0.4 ± 0.13pN |
| Polγexo- | (7.78 ± 0.29) × 10−3 | 69 ± 17 | 1.00 ± 0.10 /1 | 13 ± 2 | 1.2 ± 0.4 | 1.22 ± 0.280pN |
| Polγexo- mtSSB | (8.80 ± 0.50) × 10−3 | ≥150 | 1.23 ± 0.05 /1 | N.A. | N.A. | 0.23 ± 0.032pN |
| Polγexo-EcoSSB | (4.04 ± 0.05) × 10−3 | ≥150 | 1.39 ± 0.06 /1 | N.A. | N.A. | 0.20 ± 0.042pN |
| . | koff (s−1) (bulk) . | Nt (bulk) . | ΔGint(kBT)/M . | K(0) . | d(nm) . | Tp(f) (s nt−1) . |
|---|---|---|---|---|---|---|
| Polγ | (6.26 ± 0.05) × 10−3 | 37 ± 15 | 0.90 ± 0.10 /1 | 24 ± 3 | 1.4 ± 0.4 | 3.10 ± 0.500pN |
| Polγ mtSSB | (8.21 ± 0.14) × 10−3 | 108 ± 42 | 1.40 ± 0.04 /1 | 10 ± 3 | 1.2 ± 0.3 | 1.23 ± 0.420pN |
| Polγ EcoSSB | (3.28 ± 0.04) × 10−3 | ≥150 | 1.38 ± 0.11 /1 | N.A. | N.A. | 0.4 ± 0.13pN |
| Polγexo- | (7.78 ± 0.29) × 10−3 | 69 ± 17 | 1.00 ± 0.10 /1 | 13 ± 2 | 1.2 ± 0.4 | 1.22 ± 0.280pN |
| Polγexo- mtSSB | (8.80 ± 0.50) × 10−3 | ≥150 | 1.23 ± 0.05 /1 | N.A. | N.A. | 0.23 ± 0.032pN |
| Polγexo-EcoSSB | (4.04 ± 0.05) × 10−3 | ≥150 | 1.39 ± 0.06 /1 | N.A. | N.A. | 0.20 ± 0.042pN |
Apparent detachment rates (koff) and maximum number of replicated nucleotides (Nt) measured in strand displacement bulk assays. ΔGint/M are the minimum values of the free-parameters yielded by least squared error fits of strand displacement model to pause-free velocity data. K(0) and d are the values of free-parameters of Eq. 1 obtained upon fitting the tension dependent average residence times in pause state data (Tp(f)) with the least mean squared error. Tp(f), average residence times at pause state at the indicated tensions (superscript). In all cases, uncertainties are shown as standard errors
| . | koff (s−1) (bulk) . | Nt (bulk) . | ΔGint(kBT)/M . | K(0) . | d(nm) . | Tp(f) (s nt−1) . |
|---|---|---|---|---|---|---|
| Polγ | (6.26 ± 0.05) × 10−3 | 37 ± 15 | 0.90 ± 0.10 /1 | 24 ± 3 | 1.4 ± 0.4 | 3.10 ± 0.500pN |
| Polγ mtSSB | (8.21 ± 0.14) × 10−3 | 108 ± 42 | 1.40 ± 0.04 /1 | 10 ± 3 | 1.2 ± 0.3 | 1.23 ± 0.420pN |
| Polγ EcoSSB | (3.28 ± 0.04) × 10−3 | ≥150 | 1.38 ± 0.11 /1 | N.A. | N.A. | 0.4 ± 0.13pN |
| Polγexo- | (7.78 ± 0.29) × 10−3 | 69 ± 17 | 1.00 ± 0.10 /1 | 13 ± 2 | 1.2 ± 0.4 | 1.22 ± 0.280pN |
| Polγexo- mtSSB | (8.80 ± 0.50) × 10−3 | ≥150 | 1.23 ± 0.05 /1 | N.A. | N.A. | 0.23 ± 0.032pN |
| Polγexo-EcoSSB | (4.04 ± 0.05) × 10−3 | ≥150 | 1.39 ± 0.06 /1 | N.A. | N.A. | 0.20 ± 0.042pN |
| . | koff (s−1) (bulk) . | Nt (bulk) . | ΔGint(kBT)/M . | K(0) . | d(nm) . | Tp(f) (s nt−1) . |
|---|---|---|---|---|---|---|
| Polγ | (6.26 ± 0.05) × 10−3 | 37 ± 15 | 0.90 ± 0.10 /1 | 24 ± 3 | 1.4 ± 0.4 | 3.10 ± 0.500pN |
| Polγ mtSSB | (8.21 ± 0.14) × 10−3 | 108 ± 42 | 1.40 ± 0.04 /1 | 10 ± 3 | 1.2 ± 0.3 | 1.23 ± 0.420pN |
| Polγ EcoSSB | (3.28 ± 0.04) × 10−3 | ≥150 | 1.38 ± 0.11 /1 | N.A. | N.A. | 0.4 ± 0.13pN |
| Polγexo- | (7.78 ± 0.29) × 10−3 | 69 ± 17 | 1.00 ± 0.10 /1 | 13 ± 2 | 1.2 ± 0.4 | 1.22 ± 0.280pN |
| Polγexo- mtSSB | (8.80 ± 0.50) × 10−3 | ≥150 | 1.23 ± 0.05 /1 | N.A. | N.A. | 0.23 ± 0.032pN |
| Polγexo-EcoSSB | (4.04 ± 0.05) × 10−3 | ≥150 | 1.39 ± 0.06 /1 | N.A. | N.A. | 0.20 ± 0.042pN |
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