Rates of spontaneous lacI mutations in recA730 lexA51(Def) strains carrying plasmid-expressed genes encoding wild-type pol V or pol V_Y11A mutant
| Genotype . | lacI orientation . | Vector . | wt pol V . | pol V_Y11A . |
|---|---|---|---|---|
| rnhB+ | lacL | 3.77 (3.03–4.59) | 38.1 (34.2–42.0) | 11.3 (9.6–13.2) |
| lacR | 4.78 (3.86–5.78) | 56.4 (50.8–61.9) | 13.0 (10.9–15.2) | |
| ΔrnhB | lacL | 4.18 (3.40–5.03) | 38.2 (33.8–42.5) | 55.2 (48.9–61.3) |
| lacR | 5.22 (4.33–6.19) | 47.0 (40.8–53.2) | 54.4 (48.7–60.0) |
| Genotype . | lacI orientation . | Vector . | wt pol V . | pol V_Y11A . |
|---|---|---|---|---|
| rnhB+ | lacL | 3.77 (3.03–4.59) | 38.1 (34.2–42.0) | 11.3 (9.6–13.2) |
| lacR | 4.78 (3.86–5.78) | 56.4 (50.8–61.9) | 13.0 (10.9–15.2) | |
| ΔrnhB | lacL | 4.18 (3.40–5.03) | 38.2 (33.8–42.5) | 55.2 (48.9–61.3) |
| lacR | 5.22 (4.33–6.19) | 47.0 (40.8–53.2) | 54.4 (48.7–60.0) |
Mutation rate (×108) per lacI locus per cell division along with 95% confidence intervals in brackets was calculated as described in Materials and Methods using n = 36 cultures per strain.
Rates of spontaneous lacI mutations in recA730 lexA51(Def) strains carrying plasmid-expressed genes encoding wild-type pol V or pol V_Y11A mutant
| Genotype . | lacI orientation . | Vector . | wt pol V . | pol V_Y11A . |
|---|---|---|---|---|
| rnhB+ | lacL | 3.77 (3.03–4.59) | 38.1 (34.2–42.0) | 11.3 (9.6–13.2) |
| lacR | 4.78 (3.86–5.78) | 56.4 (50.8–61.9) | 13.0 (10.9–15.2) | |
| ΔrnhB | lacL | 4.18 (3.40–5.03) | 38.2 (33.8–42.5) | 55.2 (48.9–61.3) |
| lacR | 5.22 (4.33–6.19) | 47.0 (40.8–53.2) | 54.4 (48.7–60.0) |
| Genotype . | lacI orientation . | Vector . | wt pol V . | pol V_Y11A . |
|---|---|---|---|---|
| rnhB+ | lacL | 3.77 (3.03–4.59) | 38.1 (34.2–42.0) | 11.3 (9.6–13.2) |
| lacR | 4.78 (3.86–5.78) | 56.4 (50.8–61.9) | 13.0 (10.9–15.2) | |
| ΔrnhB | lacL | 4.18 (3.40–5.03) | 38.2 (33.8–42.5) | 55.2 (48.9–61.3) |
| lacR | 5.22 (4.33–6.19) | 47.0 (40.8–53.2) | 54.4 (48.7–60.0) |
Mutation rate (×108) per lacI locus per cell division along with 95% confidence intervals in brackets was calculated as described in Materials and Methods using n = 36 cultures per strain.
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